scholarly journals 226 Influence of trace mineral source on digestion, ruminal volatile fatty acid and soluble mineral on steers fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 133-134
Author(s):  
Octavio Guimaraes ◽  
John Wagner ◽  
Jerry Spears ◽  
Terry Engle
Keyword(s):  
Dairy Cow ◽  
Rumen Fluid ◽  
Adaptation Period ◽  
Trace Mineral ◽  
Specific Amino Acid ◽  
Fiber Digestion ◽  
Post Feeding ◽  
Pulse Dose ◽  
The Impact ◽  
Cu And Zn

Abstract Twelve Angus steers (BW 530.9 ± 22.7kg) fitted with ruminal cannulae were used to determine the impact of trace mineral source on digestibility, ruminal VFA, and soluble concentrations of Cu, Zn, and Mn. Steers were fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow (DM basis: 16.8% CP, 29.2 Mcal NEm, 30.3 Mcal NEl, 33.6% NDF) for 21 d. Treatments consisted of 10 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either sulfate (STM), hydroxy (HTM; IntelliBond) or organic (ORG; metal specific amino acid complexes) sources (n=4 steers/treatment). Following a 21-d adaptation period, total fecal output was collected for 5 d. On d 6, rumen fluid was collected at 0, 2, and 4 h post feeding and analyzed for VFA. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose (cannulae) of 100 mg Cu, 400 mg Mn, and 60 mg Zn from either STM, HTM, or ORG. Ruminal samples were obtained at 2-h intervals starting at -4 and ending at 24 h post dosing and analyzed for soluble Cu, Mn, and Zn. Digestibility of NDF and ADF were lesser (P < 0.05) in STM vs. HTM and ORG supplemented steers. Steers receiving HTM and ORG had greater (P < 0.05) total VFA concentrations than STM supplemented steers at 2 and 4h post feeding. Ruminal soluble Cu and Zn concentrations were greater (P < 0.001) post dosing in STM and ORG supplemented steers at 2, 4, and 6 h for Cu and 4, 6, 8, 10 and 12 h for Zn when compared to HTM supplemented steers. Results indicate that ruminal solubility of Cu and Zn differs between STM, HTM and ORG sources and trace mineral source impacts fiber digestion and ruminal VFA concentrations.

scholarly journals Trace mineral source impacts rumen trace mineral metabolism and fiber digestion in steers fed a medium-quality grass hay diet

2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Octavio Guimaraes ◽  
Sam Jalali ◽  
John J Wagner ◽  
Jerry W Spears ◽  
Terry Eugene Engle
Keyword(s):  
Mineral Metabolism ◽  
Neutral Detergent Fiber ◽  
Adaptation Period ◽  
Trace Mineral ◽  
Time Interaction ◽  
Grass Hay ◽  
Medium Quality ◽  
Mineral Concentrations ◽  
The Impact ◽  
Cu And Zn

Abstract Twelve Angus steers (BW 452.8 ± 6.1 kg) fitted with ruminal cannulae were used to determine the impact of trace mineral (TM) source on digestibility, ruminal volatile fatty acid (VFA) composition, ruminal soluble concentrations of Cu, Zn, and Mn, and relative binding strength of trace minerals located in the rumen insoluble digesta fraction. Steers were fed a medium-quality grass hay diet (DM basis: 10.8% CP, 63.1% neutral detergent fiber [NDF], 6.9 mg Cu/kg, 65.5 mg Mn/kg, and 39.4 mg Zn/kg) supplemented with protein for 21 d. Treatments consisted of either sulfate (STM) or hydroxy (HTM) sources (n = 6 steers/treatment) to provide 20, 40, and 60 mg supplemental Cu, Mn, and Zn/kg DM, respectively. Following a 21-d adaptation period, total fecal output was collected for 5 d. Dry matter (P < 0.07) and CP (P < 0.06) digestibility tended to be reduced, and NDF (P < 0.04) and acid detergent fiber (ADF) (P < 0.05) digestibility were reduced in STM- vs. HTM-supplemented steers. On day 6, ruminal fluid was collected at 0, 2, and 4 h post-feeding and analyzed for VFA. There were no treatment x time interactions for VFA. Steers receiving HTM had less (P < 0.02) molar proportions of butyric acid and greater (P < 0.05) total VFA concentrations than STM-supplemented steers. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On day 15 steers received a pulse dose of 20 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either STM or HTM (n = 6 steers/treatment). Ruminal samples were obtained at 2-h intervals starting at −4 and ending at 24 h relative to dosing. There was a treatment x time interaction (P < 0.03) for ruminal soluble Cu, Mn, and Zn concentrations. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 14 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post-dosing in STM compared with HTM-supplemented steers. Copper concentrations were greater (P < 0.05) at 12 and 24 h and Zn concentrations in ruminal solid digesta were greater at 24 h in HTM-supplemented steers. Upon dialysis against Tris-EDTA, the percent Zn released from digesta was greater (P < 0.05) at 12 h (P < 0.03) and 24 h (P < 0.05), and the percent Cu released was greater (P < 0.02) at 24 h post-dosing in HTM steers when compared with STM-supplemented steers. Results indicate that Cu and Zn from HTM have low solubility in the rumen and appear to be less tightly bound to ruminal solid digesta than Cu and Zn from STM. The lower ruminal soluble concentrations of Cu and Zn in steers given HTM were associated with greater fiber digestibility.

scholarly journals 414 The influence of trace mineral source on fiber digestion, rumen fermentation characteristics, and mineral solubility in beef cattle fed a low-quality forage diet

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 167-167 ◽  
Author(s):  
Octavio Guimaraes ◽  
Sam Jalali ◽  
John Wagner ◽  
Jerry Spears ◽  
Terry Engle
Keyword(s):  
Rumen Fluid ◽  
Neutral Detergent Fiber ◽  
Adaptation Period ◽  
Ruminal Fermentation ◽  
Trace Mineral ◽  
Mineral Solubility ◽  
Dry Matter Digestibility ◽  
Grass Hay ◽  
Mineral Concentrations ◽  
The Impact

Abstract Twelve Angus steers (BW 452.8 ±21.8 kg) fitted with ruminal cannulae were used to determine the impact of trace mineral source on neutral detergent fiber (NDF) digestibility, short chain fatty acid (SCFA) production, and ruminal solubility of Cu, Mn, and Zn. Steers were fed a low-quality grass hay diet (10.8% CP, 63.1% NDF, 6.9 mg Cu/kg, 65.5 mg Mn/kg, and 39.4 mg Zn/kg) supplemented with protein for 21 d. Treatments consisted of 20, 40, and 60 mg supplemental Cu, Mn, and Zn/kg DM, respectively from either sulfate (STM) or hydroxy (HTM) sources (n = 6 steers/treatment). Following a 21-d adaptation period total fecal output was collected for 5 d. Dry matter digestibility tended (P < 0.07) to be reduced (51.9 vs. 53.4 ±0.52%) and NDF digestibility was reduced (P < 0.04; 40.4 vs. 42.7 ±0.67%) in STM vs. HTM steers. On d 6, rumen fluid was collected at 0, 2, and 4h post feeding. Steers receiving HTM had lesser (P < 0.02) butyric acid and greater (P < 0.05) total SCFA than STM steers. Steers were then fed the same grass hay diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose of 20, 40, and 60 mg Cu, Mn, and Zn/kg DM, respectively from either STM or HTM. Ruminal samples were obtained at 2h intervals starting at -4 and ending at 24h relative to dosing. Ruminal soluble mineral concentrations were greater (P < 0.05) for Cu at 4, 6, 8, 10, 12, and 14h; for Mn at 4 and 6h; and for Zn at 4, 6, and 8h post dosing in STM compared to HTM steers. Results indicate that ruminal solubility of Cu, Mn, and Zn differs between STM and HTM sources and rumen Cu, Mn, and Zn solubility may impact ruminal fermentation.

143 Effect of Supplementary Selenium Source on Dairy Cow Performance, Antioxidant Status, and Apparent Absorption and Retention

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 80-80
Author(s):  
Keira M Cruickshank ◽  
Bayissa Hatew ◽  
Amanda M Gehman ◽  
Karen M Koenig ◽  
Eduardo S Ribeiro ◽  
...  
Keyword(s):  
Dairy Cow ◽  
Fixed Effects ◽  
Antioxidant Status ◽  
Treatment Time ◽  
Rumen Fluid ◽  
Random Effect ◽  
Fecal Excretion ◽  
Adaptation Period ◽  
Apparent Absorption ◽  
Cow Performance

Abstract The objective of this experiment was to determine how source of selenium (Se) affects dairy cow performance, antioxidant status, and apparent absorption and retention. Multiparous Holstein cows (n = 24; 597 ± 49 kg BW) were blocked by days in milk (161 ± 18) and randomly assigned to receive 0.3 mg/kg of either: 1) organic Se (selenized yeast; ORG; Sel-Plex-Alltech); or, 2) inorganic Se (sodium selenite; INO) premix, top dressed and mixed into a ration daily. After an 11-week adaptation period (blood and milk sampled monthly), cows received an intraruminal dose of Se77 (either Se77 yeast or NaSe77O3) followed by a 4-day period of blood and rumen fluid sampling, and total collection of feces, urine, and milk. Data were analyzed using PROC GLIMMIX in SAS with fixed effects of treatment, time, and their interaction, and random effect of block. Daily DMI (23 ± 0.6 kg), milk yield (35 ± 1.2 kg), plasma glutathione peroxidase (64 ± 4.2 U), and serum Se (0.11 ± 0.003 µg/g) were not different between treatments during the adaptation period. Serum Se77 maximum concentration and area under the curve (AUC) were not different between treatments for 72 hours following infusion, but rumen fluid Se77 AUC was higher (P = 0.02) for ORG cows. Apparent absorption (64 ± 1.3%) and retention (44 ± 1.5%) of the Se77 dose did not differ between treatments. The ORG cows had lower urinary excretion (13 vs. 17 ± 0.6%; P < 0.01), higher milk excretion (6 vs. 2 ± 0.3%; P < 0.01), and similar fecal excretion (36 ± 1.3%; P = 0.9) of Se77 compared to INO cows. These results indicate that ORG Se increased Se content of milk and decreased Se excretion in urine, but did not alter performance, antioxidant status, and apparent absorption and retention of Se in mid-lactation cows.

Increasing magnesium intakes in relation to magnesium absorption in dry cows

2004 ◽  
Vol 71 (3) ◽  
pp. 297-303 ◽  
Author(s):  
Surasak Jittakhot ◽  
J Thomas Schonewille ◽  
Hugo Wouterse ◽  
Anton WJ Uijttewaal ◽  
Chalermpon Yuangklang ◽  
...  
Keyword(s):  
Dairy Cow ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Basal Diet ◽  
Apparent Absorption ◽  
Post Feeding ◽  
Saturation Kinetics ◽  
Kinetics Of

Earlier studies with temporarily isolated rumen of heifers show saturation kinetics of Mg efflux across the rumen wall. Therefore, we hypothesized that high Mg intakes would not further increase the rate of Mg absorption in cows. To test our hypothesis, six ruminally fistulated non-pregnant dry cows were given diets with different Mg concentrations in a 6×6 Latin square design. Desired concentrations of Mg were attained by adding MgO to the basal diet and the Mg concentrations in the total rations were 3·8, 6·4, 9·1, 11·8, 14·1 and 17·3 g Mg/kg dry matter, which provided Mg intakes of 27·1, 44·6, 64·6, 83·5, 100·4 and 124·3 g/d, respectively. Increasing Mg intakes were associated with increased (P<0·001) faecal Mg excretion. However, apparent Mg absorption expressed as g/d was not significantly different for Mg intakes from 100·4 to 124·3 g/d while Mg absorption expressed as a proportion of intake was not significantly different for Mg intakes ranging from 64·6 to 124·3 g/d. Mg concentrations in rumen fluid after feeding increased (P<0·001) with increasing Mg intakes. Apparent absorption of Mg appeared to become saturated at a ruminal Mg concentration of 17·5 mM (Mg intake of 83·5 g/d). Group-mean post-feeding concentrations of Mg and Na in rumen fluid were significantly correlated (Pearson's r=−0·96; P=0·003, n=6). This study showed that under conditions of practical dairy cow feeding, Mg absorption was maximal at Mg intakes [ges ]84 g/d.

scholarly journals PSXII-13 The influence of trace mineral source on copper, manganese, and zinc binding strength to rumen digesta in cattle fed a high forage-based diet

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 443-443
Author(s):  
Octavio Guimaraes ◽  
Huey Yi Loh ◽  
Meghan P Thorndyke ◽  
Nicole M Tillquist ◽  
Jerry W Spears ◽  
...  
Keyword(s):  
Insoluble Fraction ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Binding Strength ◽  
Pulse Dose ◽  
Copper Manganese ◽  
High Forage ◽  
Mineral Concentrations ◽  
Cu And Zn ◽  
Mn Concentrations

Abstract Twelve crossbred Angus steers fitted with rumen cannulae were used to determine the influence of trace mineral source on ruminal soluble mineral concentrations and relative binding strength of trace minerals in the ruminal insoluble digesta fraction. Steers were adjusted to a low-quality hay (chopped) diet balanced to meet the nutrient requirements for growing steers. For 7 d prior to initiation of the study, steers were fed the diet without supplemental Cu, Mn, and Zn. On day 8 steers received a pulse dose (2 x NRC requirement) of Cu, Mn, and, Zn from sulfate (STM) or hydroxy trace minerals (HTM; Micronutrients LLC, USA). Ruminal samples were obtained at 2 h intervals until 24 h post-dosing for soluble (fraction obtained after centrifugation) Cu, Mn, and Zn concentrations. Binding strength of Cu, Mn, and Zn to ruminal solid digesta (post centrifugation) was estimated at 0, 12, and 24h post-dosing using dialysis against 0.05 M Tris-EDTA. Ruminal soluble mineral concentrations were greater (P &lt; 0.05) for Cu at 4, 6, 8, 10, 12, and 16 h; for Mn at 4 and 6 h; and for Zn at 4, 6, and 8 h post dosing in STM compared to HTM steers. Concentration of Cu, Mn, and Zn remaining in the insoluble fraction were greater (P &lt; 0.05) at 12 h post dosing in HTM vs STM supplemented steers. At 24 h post dosing, Mn concentrations were lesser (P &lt; 0.03) and Zn concentrations were greater (P &lt; 0.001) in HTM vs STM supplemented steers. Following dialysis, the % Cu, and Zn released from the insoluble fraction was greater (P &lt; 0.001) at 12 h and 24h in HTM vs. STM supplemented steers. Results indicate that a greater proportion of Cu and Zn HTM remained loosely bound to the insoluble fraction within the rumen.

scholarly journals 393 Awardee Talk: Effect of trace mineral source on rumen fermentation and trace mineral distribution in the rumen

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 171-172
Author(s):  
Terry E Engle
Keyword(s):  
Small Intestine ◽  
Insoluble Fraction ◽  
Absorption Efficiency ◽  
Future Research ◽  
Microbial Fermentation ◽  
Trace Mineral ◽  
Passage Rate ◽  
Rumen Microorganisms ◽  
Fiber Digestion ◽  
Cu And Zn

Abstract The role that Cu and Zn play in rumen microbial fermentation is not well understood. Microorganisms use small proportions of dietary Cu and Zn for catalytic, structural, and stabilizing functions. In order for microorganisms to acquire Cu and Zn, the elements must be soluble in the rumen environment. Data would indicate that practical diets fed to ruminants without Cu and Zn supplementation are able to meet the microbial Cu and Zn requirements. Therefore, rumen solubility of supplemental Cu and Zn can impact rumen microbial fermentation characteristics. Numerous factors can impact rumen solubility of minerals, such as the pH of the rumen, the concentration of dietary antagonists (Mo, Fe, S, fiber, etc.), and mineral source. Earlier research has indicated that high soluble concentrations of Cu and Zn in the rumen environment can become toxic to certain rumen microorganisms and decrease fiber digestion. Recent research from our laboratory has indicated that hydroxy trace mineral (HTM) forms of Cu and Zn are relatively insoluble in the rumen and that a greater proportion of HTM remained loosely bound to the insoluble fraction within the rumen when compared to sulfate trace mineral (STM) sources of Cu and Zn. We have also reported that supplemental sources of Cu and Zn that are highly soluble in the rumen environment (STM) can decrease fiber digestion, reduce total VFA concentrations, and can become more tightly bound to rumen solid digesta than HTM sources of Cu and Zn. The stronger binding of STM relative to HTM to the solid rumen digesta fraction may reduce absorption in the small intestine. Future research investigating the flow and passage rate of different trace mineral sources through the abomasum into the duodenum and duodenal absorption efficiency of Cu and Zn from different TM sources is needed.

PSX-A-12 Late-Breaking: Effects of Renergy™ on ruminal fermentation in steers fed a high-grain diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 375-375
Author(s):  
Jeff S Heldt ◽  
Harrison Hallmark ◽  
Huey Yi Loh ◽  
Sara Crane ◽  
Briana V Tangredi ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Rumen Fluid ◽  
Lactate Production ◽  
Experimental Unit ◽  
Adaptation Period ◽  
Ruminal Fermentation ◽  
Acetate Production ◽  
Ruminal Ph ◽  
Post Feeding ◽  
Dietary Treatments

Abstract Renergy™ is a proprietary blend of organic acids with a proposed mode of action of increasing ruminal propionate production. Little is known about the efficacy of Renergy™ supplementation in modifying ruminal fermentation in beef cattle consuming high-grain diets. Therefore, eight Angus steers (BW 531.7 ± 20.4 kg) fitted with ruminal cannulae were used to determine the effects of Renergy™ on ruminal fermentation characteristics. Steers were fed a high concentrate diet (DM basis: 13.6% CP, 1.38 Mcal/kg NEg, and 2.02 Mcal/kg NEm) with no monensin sodium or tylosin phosphate added to the diet for 30 d prior to the initiation of the experiment. Treatments consisted of control (CON; no supplemental Renergy™) and Renergy (REN) fed at 27.6 g.animal-1.d-1 (n = 4 steers/treatment; experimental unit = animal). Following the 30 d diet adaptation period, dietary treatments were initiated for 28-d. On day 28, rumen fluid was collected at 3 h post feeding and analyzed for VFA, pH, and NH3. Ruminal pH (P = 0.62) and NH3 (P = 0.56) were unaffected by treatment. However, total VFA (P = 0.05) and propionate (P = 0.03) production were increased by Renergy, 13.3% and 25.7% respectively. There was a tendency (P = 0.14) for acetate production to be increased 10.9% in steers supplemented with Renergy™. Butyrate was unaffected (P = 0.51) by treatment. However, isobutyrate production was lower (P &lt; 0.01) in steers receiving Renergy™. Feeding Renergy™ also resulted in 25% less (P = 0.07) L-lactate production. Under conditions of this experiment, results indicate that supplementing Renergy™ for 28d to beef cattle consuming high concentrate diets impacts ruminal fermentation 3 h post feeding.

Advancing dairy farming profitability through research

2001 ◽  
pp. 17-22
Author(s):  
J.R. Caradus ◽  
D.A. Clark
Keyword(s):  
Research And Development ◽  
Dairy Cow ◽  
Low Cost ◽  
Pasture Management ◽  
Nitrogen Fertiliser ◽  
Grass Endophyte ◽  
Forage Species ◽  
Development Outcomes ◽  
Dairy Cow Nutrition ◽  
The Impact

The New Zealand dairy industry recognises that to remain competitive it must continue to invest in research and development. Outcomes from research have ensured year-round provision of low-cost feed from pasture while improving productivity. Some of these advances, discussed in this paper, include the use of white clover in pasture, understanding the impacts of grass endophyte, improved dairy cow nutrition, the use of alternative forage species and nitrogen fertiliser to improve productivity, demonstration of the impact of days-in-milk on profitability, and the use of feed budgeting and appropriate pasture management. Keywords: dairy, profitability, research and development

scholarly journals The influence of xylanase on the fermentability, digestibility, and physiochemical properties of insoluble corn-based fiber along the gastrointestinal tract of growing pigs

2021 ◽  
Author(s):  
Amy L Petry ◽  
Nichole F Huntley ◽  
Michael R Bedford ◽  
John F Patience
Keyword(s):  
Mixed Model ◽  
Linear Mixed Model ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Neutral Detergent Fiber ◽  
Adaptation Period ◽  
High Fiber ◽  
Gross Energy ◽  
Digesta Viscosity ◽  
The Impact

Abstract In theory, supplementing xylanase in corn-based swine diets should improve nutrient and energy digestibility and fiber fermentability, but its efficacy is inconsistent. The experimental objective was to investigate the impact of xylanase on energy and nutrient digestibility, digesta viscosity, and fermentation when pigs are fed a diet high in insoluble fiber (&gt;20% neutral detergent fiber; NDF) and given a 46-d dietary adaptation period. Three replicates of 20 growing gilts were blocked by initial body weight, individually housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF) with 7.5% NDF, a 30% corn bran high-fiber control (HF; 21.9% NDF), HF+100 mg xylanase/kg [HF+XY, (Econase XT 25P; AB Vista, Marlborough, UK)] providing 16,000 birch xylan units/kg; and HF+50 mg arabinoxylan-oligosaccharide (AXOS) product/kg [HF+AX, (XOS 35A; Shandong Longlive Biotechnology, Shandong, China)] providing AXOS with 3-7 degrees of polymerization. Gilts were allowed ad libitum access to fed for 36-d. On d 36, pigs were housed in metabolism crates for a 10-d period, limit fed, and feces were collected. On d 46, pigs were euthanized and ileal, cecal, and colonic digesta were collected. Data were analyzed as a linear mixed model with block and replication as random effects, and treatment as a fixed effect. Compared with LF, HF reduced the apparent ileal digestibility (AID), apparent cecal digestibility (ACED), apparent colonic digestibility (ACOD), and apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), crude protein (CP), acid detergent fiber (ADF), NDF, and hemicellulose (P&lt;0.01). Relative to HF, HF+XY improved the AID of GE, CP, and NDF (P&lt;0.05), and improved the ACED, ACOD, and ATTD of DM, GE, CP, NDF, ADF, and hemicellulose (P&lt;0.05). Among treatments, pigs fed HF had increased hindgut DM disappearance (P=0.031). Relative to HF, HF+XY improved cecal disappearance of DM (162 vs. 98g; P=0.008) and NDF (44 vs. 13g; P&lt;0.01). Pigs fed xylanase had a greater proportion of acetate in cecal digesta and butyrate in colonic digesta among treatments (P&lt;0.05). Compared with LF, HF increased ileal, cecal, and colonic viscosity, but HF+XY decreased ileal viscosity compared with HF (P&lt;0.001). In conclusion, increased insoluble corn-based fiber decreases digestibility, reduces cecal fermentation, and increases digesta viscosity, but supplementing xylanase partially mitigated that effect.

scholarly journals 129 Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on growth performance, nutrient digestibility, and colonic pH in diets fed to weanling pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 101-102
Author(s):  
Jaron R Lewton ◽  
Adrienne Woodward ◽  
Ronny Moser ◽  
Kyan M Thelen ◽  
Adam J Moeser ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Distal Colon ◽  
Nutrient Digestibility ◽  
Nursery Pigs ◽  
Fiber Digestion ◽  
Males And Females ◽  
Dietary Treatments ◽  
The Impact

Abstract A study was conducted to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on apparent digestibility and colonic pH of nursery pigs. Eighty pigs, of equal number of barrows and gilts (initial BW: 6.99 ± 1.67 kg), were weaned at 21 ± 1 d and randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a control plus DFM (DFM). Both diets were corn, soybean meal, and distillers dried grains based, formulated to meet all or exceed all nutritional requirements, and manufactured on site. Diets were fed for 42 days. Performance measures were recorded weekly. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Digestibility of specific nutrients was evaluated within the duodenum, jejunum, ileum, ascending and distal colon. There were no overall differences in growth performance. Overall means ± SD were 0.51 ± 0.05 kg/d, 0.79 ± 0.05 kg/d and 0.66 ± 0.05 for ADG, ADFI, and G:F, respectively. Digestibility of tryptophan within the jejunum tended (P = 0.06) to increase with addition of DFM, as did cysteine (P = 0.12) and methionine (P = 0.10). The analysis also suggested that the impact of the DFM on the digestibility of amino acids may be early in the nursery phase. The pH of contents in ascending colon, a possible indicator of varied fiber digestion, did not differ. Likewise, no differences were observed between treatment in apparent total tract nitrogen and energy digestibility (analysis of distal colon contents). The addition of a multi-strain Bacillus subtilis-based DFM appears to impact digestibility of select amino acids depending upon location in the gastrointestinal tract.

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