Optimum grape pomace proportion in feedlot cattle diets: ruminal fermentation, total tract nutrient digestibility, nitrogen utilization, and blood metabolites

Abstract Because of its high content of polyphenolic compounds, the dietary inclusion of grape pomace (GP) in ruminant diets can reduce reactive nitrogen (N) and methane emissions and enhance the shelf life and beneficial fatty acids (FAs) content of meat. However, the dietary inclusion of GP beyond a threshold that is still to be determined for feedlot cattle can also compromise nutrient supply and, thus, growth performance. This study investigated the optimum proportion of GP in finishing cattle diets. Nutrient intake and apparent total tract digestion, ruminal pH and fermentation, estimated microbial protein synthesis, route of N excretion, and blood metabolites were measured. Six ruminally fistulated crossbred beef heifers (mean initial body weight ± SD: 714 ± 50.7 kg) were used in a replicated 3 × 3 Latin square with 21-d periods. Dietary treatments were 0%, 15%, and 30% of dietary dry matter (DM) as GP, with diets containing 84%, 69%, and 54% dry-rolled barley grain, respectively. There was a linear increase (P = 0.07) in DM intake and quadratic change (P ≤ 0.01) in neutral detergent fiber (NDF) intake. There was a quadratic change (P ≤ 0.04) in apparent total tract DM, NDF, and crude protein digestibility as dietary GP content increased. However, there were no treatment effects (P ≥ 0.18) on total ruminal short-chain FA concentration and duration and area pH < 6.2, 5.8, and 5.5. Although N intake did not differ (269, 262, 253 g/d; P = 0.33) across dietary treatments, feeding GP led to a tendency for a quadratic change (P ≤ 0.07) in ruminal ammonia-N and plasma urea-N concentrations. Total N excretion also changed (quadratic, P = 0.03) because of changes (quadratic, P = 0.02) in fecal N excretion as urinary excretion of N and urea-N did not differ (P ≥ 0.15) across treatments. Feeding GP led to quadratic changes (P ≤ 0.01) in fecal excretion of fiber-bound N. Microbial N flow and apparent N retention also changed (quadratic, P ≤ 0.04) as dietary GP proportion increased. In conclusion, responses to dietary GP proportion were mostly quadratic with indications that nutrient supply as reflected by changes in apparent total tract nutrient digestibility, microbial N supply, and apparent N retention could be compromised beyond a 15% dietary inclusion level.

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Feeding either palm oil or sunflower oil on nutrient digestibility and blood metabolites in crossbred Thai native x Brahman bull

Indian Journal of Animal Research ◽

10.18805/ijar.8419 ◽

2016 ◽

Author(s):

Wantanee Polviset ◽

Nattiya Prakobsaeng

Keyword(s):

Beef Cattle ◽

Palm Oil ◽

Sunflower Oil ◽

Low Density Lipoprotein ◽

Latin Square ◽

Density Lipoprotein ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Blood Metabolites ◽

Dietary Treatments

This experiment was conducted to investigate the effect of palm oil and sunflower oil supplementations on digestibility and blood metabolites in crossbred Thai native x brahman bulls fed on TMR used rice straw as a roughage source. Four, 2 year old crossbred Thai native x Brahman bulls were randomly assigned in a 2x2 factorial in 4 x 4 latin square design. Each period of feeding lasted for 21 days to receive four dietary treatments; 3% palm oil, 6% palm oil, 3% sunflower oil and 6% sunflower oil. All animals were fed with TMR ad-libitum. It was found that supplementation of palm oil and sunflower oil had no effect on digestibility of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF) and crude protein (CP). Supplementation resulted in significant (P<0.05) lower acid detergent fiber (ADF) digestibility when feeding with palm oil on the other hand feeding beef cattle with 6% from palm oil and sunflower oil were higher Ether extract (EE) digestibility than 3% from twice oils (P<0.05). Blood glucose, blood urea nitrogen, cholesterol, triglyceride, high density lipoprotein and low density lipoprotein were not significant different among treatments. Based on this study, supplementing TMR sunflower oil in diets was suitable in beef cattle without any effect ruminal digestibility and blood metabolites.

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The Inclusion of Black Cumin Meal Improves Growth Performance of Growing Awassi Lambs

Veterinary Sciences ◽

10.3390/vetsci7020040 ◽

2020 ◽

Vol 7 (2) ◽

pp. 40

Author(s):

Belal S. Obeidat

Keyword(s):

Growth Performance ◽

Nigella Sativa ◽

Average Daily Gain ◽

Nutrient Digestibility ◽

Metabolizable Energy ◽

Neutral Detergent Fiber ◽

Black Cumin ◽

Daily Body Weight ◽

Dietary Treatments ◽

Awassi Lambs

Twenty-four Awassi lambs were randomly divided into two dietary treatments to assess the influence of black cumin meal (BCM; Nigella sativa L.) feeding on growth performance. Diets were no BCM (CON) or 150 g BCM/kg DM (BCM150)). Lambs were fed the experimental diets for 80 days. Lambs were housed randomly in individual pens that were fitted with water and feed containers. During the study, nutrient intake was measured daily. Body weight (BW) and average daily gain (ADG) were evaluated biweekly. Nutrient digestibility and nitrogen (N) balance were evaluated on days 49–59. Intakes of dry matter, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), ether extract (EE) and metabolizable energy were greater (p ≤ 0.05) in the BCM150 diet than in the CON diet. The digestibility of DM, CP and EE (p ≤ 0.04) was improved in the BCM150 diet compared to the CON diet. However, NDF and ADF digestibility did not differ between the two diets. Nitrogen retained (g/d; p = 0.01) and N retention (%; p = 0.05) was greater in the BCM150 compared with CON diet. Final BW, ADG, and feed efficiency (DM intake: kg of gain) was greater (p ≤ 0.002) in BCM150 lambs than the CON lambs. However, cost/kg of BW gain was lower in the BCM150 diet than in the CON diet. In conclusion, the inclusion of black cumin meal improved the growth performance and profits in diets of growing lambs. Therefore, it could be used as an alternative to soybean meal and barley as a protein and energy supplement, respectively.

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Substituting corn silage with reconstituted forage or nonforage fiber sources in the starter diets of Holstein calves: effects on performance, ruminal fermentation, and blood metabolites

Journal of Animal Science ◽

10.1093/jas/skz180 ◽

2019 ◽

Vol 97 (7) ◽

pp. 3046-3055 ◽

Cited By ~ 2

Author(s):

Shahryar Kargar ◽

Meysam Kanani ◽

Marzia Albenzio ◽

Mariangela Caroprese

Keyword(s):

Growth Performance ◽

Nutrient Intake ◽

Rumen Fluid ◽

Nutrient Digestibility ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Daily Weight Gain ◽

Blood Metabolites ◽

Albumin Concentration ◽

Volatile Fatty Acid Concentration

Abstract We examined the effects of replacing corn silage (CS) with reconstituted alfalfa hay (AH) or beet pulp (BP) in the starter diet on the nutrient intake and digestibility, growth performance, rumen fermentation characteristics, selected blood metabolites, and health status in Holstein dairy calves. Newborn female calves (n = 54; 3 d of age; 39.8 ± 1.36 kg BW) were assigned randomly to 3 groups receiving starter diets containing CS [10% dry matter (DM) basis; CS diet) and reconstituted AH (10% DM, RAH diet) or BP (10% DM; RBP diet). The starter diets had the same nutrient composition and DM content. The calves were weaned on day 50 and the study continued until day 70. Nutrient intake, body weight (at weaning and at the end of the study), daily weight gain, feed efficiency, and body measurements (including heart girth, withers height, body length, body barrel, hip height, and hip width) were not affected by the diet (P > 0.05). Health-related variables including rectal temperature, fecal score, and general appearance score were not influenced by the diets (P > 0.05). During the postweaning period, apparent total tract digestibility of DM, organic matter, and crude protein were higher for RBP (P = 0.001); however, digestibility of neutral detergent fiber was lower in RAH compared with CS or RBP (P = 0.001). Daily amount of nutrient digestibility did not change across the diets (P > 0.05). Rumen fluid pH and total volatile fatty acid concentration and profile were not different across the diets after weaning (P > 0.05). Calves fed RAH or RBP had higher blood concentration of β-hydroxy butyric acid compared with CS only before weaning (P = 0.03). Blood albumin concentration was higher for RBP compared with CS or RAH during the preweaning (P = 0.006) and overall (P = 0.005) periods; however, it was lower for CS compared with RBP after weaning (P = 0.03). Concentration of other blood variables including glucose, blood urea N, total protein, and globulin did not change across the diets (P > 0.05). Calves, in general, were healthy, and replacing CS with RAH or RBP in the starter diet had no beneficial effect on their feed intake or growth performance indicating that CS and reconstituted AH or BP can be used interchangeably in dairy calf starter diets until 70 d of age, allowing dairy producers more choices in selecting the feed ingredients.

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Replacing Soybean Meal with Sesame Meal in the Diets of Lactating Awassi Ewes Suckling Single Lambs: Nutrient Digestibility, Milk Production, and Lamb Growth

Animals ◽

10.3390/ani9040157 ◽

2019 ◽

Vol 9 (4) ◽

pp. 157 ◽

Cited By ~ 5

Author(s):

Belal S. Obeidat ◽

Rami T. Kridli ◽

Kamel Z. Mahmoud ◽

Mohammed D. Obeidat ◽

Serhan G. Haddad ◽

...

Keyword(s):

Milk Production ◽

Soybean Meal ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

N Balance ◽

Sesame Meal ◽

Ewe Lambs ◽

Diet Cost ◽

Dietary Treatments ◽

Lamb Growth

Two experiments were conducted to assess the influence of sesame meal (SM) feeding on nutrient digestibility, N balance, milk production and composition, ewes’ body weight change, and growth performance of lambs. In experiment 1, 18 ewe lambs were randomly distributed into three diets to evaluate the effects of soybean meal replacement with SM on nutrient intake, digestibility, and N balance. Treatments were no SM (SM0), 7.5% SM (SM7.5), or 15% SM (SM15) of the dietary dry matter (DM). Aside from intake and digestibility of ether extract (EE), which was greater in the SM-containing diets compared with SM0, intake and digestibility of the remaining nutrients was similar among dietary treatments. In experiment 2, 30 ewes suckling single lambs were randomly assigned to the same diets used in experiment 1. Intakes of DM, crude protein, neutral detergent fiber, and acid detergent fiber were unaffected by treatment. Milk yield was greater in SM diets than in the SM0 diet. Cost/kg of milk production decreased while feed efficiency improved in the SM-diets compared to the SM0 diet. In conclusion, results of the current studies demonstrate the possibility of replacing soybean meal with sesame meal in diets of lactating Awassi ewes.

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Effects of Partial Replacement of Conventional with Alternative Feeds on Nutrient Intake, Digestibility, Milk Yield and Composition of Awassi Ewes and Lambs

Animals ◽

10.3390/ani9090684 ◽

2019 ◽

Vol 9 (9) ◽

pp. 684 ◽

Cited By ~ 2

Author(s):

Mohammad K. Aloueedat ◽

Belal S. Obeidat ◽

Mofleh S. Awawdeh

Keyword(s):

Milk Yield ◽

Dietary Treatment ◽

Milk Composition ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Partial Replacement ◽

Fiber Intake ◽

N Retention ◽

Distillers Grains With Solubles ◽

Alternative Feeds

Two experiments were done to assess the effects of alternative feeds (AF; dried distillers grains with solubles, carob pods, olive cake, and bread by-product) on lactating performance of ewes and digestibility and nitrogen (N) retention of lambs. Diets were: no AF (CON); 200 g/kg AF (AF200); and 400 g/kg AF (AF400). In Experiment 1, 27 Awassi ewes were randomly distributed into three groups, and each was fed one of the diets described before (9 ewes/diet). Evaluation of milk composition and yield was performed at the beginning of the experiment and on days 18, 36, and 54. In Experiment 2, 18 Awassi lambs were allocated to the same diets (6 ewe lambs/diet) during a 21-day trial (14 days housed individually in shaded pens and 7 days in metabolic cages). In Experiment 1, no differences in body weight (BW) of the ewes and their lambs were detected. With the exception of neutral detergent fiber intake, which was lower in the AF-containing diets compared with CON, dry matter, crude protein, and acid detergent fiber intake were not affected by dietary treatment. Milk yield and composition was comparable among diets, and the cost of milk production was lower in AF compared to CON diet. In Experiment 2, nutrient digestibility and N retention were not affected by the diet. Results showed the possibility of including different AF in ewe and lamb diets to mitigate production cost without negatively affecting intake, milk yield, and composition, digestibility, and animal welfare and health.

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Effect of Physical Form and Level of Wheat Straw Inclusion on Growth Performance and Blood Metabolites of Fattening Goat

Animals ◽

10.3390/ani10101861 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1861

Author(s):

Muhammad I. Malik ◽

Muhammad A. Rashid ◽

Muhammad S. Yousaf ◽

Saima Naveed ◽

Khalid Javed ◽

...

Keyword(s):

Growth Performance ◽

Wheat Straw ◽

Hematological Parameters ◽

Average Daily Gain ◽

Neutral Detergent Fiber ◽

Blood Metabolites ◽

Ruminal Acidosis ◽

Physical Form ◽

Dietary Treatments ◽

Daily Gain

The inclusion of straw in high concentrate total mixed rations (TMRs) of male fattening goats can provide the necessary fiber to prevent ruminal acidosis and maintain growth. The objective of this study was to evaluate the effects of the physical form (PF) of the diet (pelleted vs. conventional) and the straw level (SL) of wheat straw (WS) (15% versus 25%) in total mixed rations on feed intake, growth, total tract digestibility, and blood metabolites of fattening goats. Thirty-two male Beetal goats (27.4 ± 0.28 kg body weight (BW)) were divided randomly into the following four dietary treatments with a 2 × 2 factorial arrangement (n = 8/treatment): (1) CTMR15 (conventional TMR containing 15% WS), (2) CTMR25 (conventional TMR containing 25% WS), (3) PTMR15 (pelleted TMR containing 15% WS), and (4) PTMR25 (pelleted TMR containing 25% WS). Both conventional and pelleted 15% WS TMR had 33.7% neutral detergent fiber (NDF) and 19.3% acid detergent fiber (ADF), whereas in 25% WS TMR the NDF and ADF contents were 38.7% and 22.9%, respectively. The experimental diets were formulated to be iso-nitrogenous (crude protein (CP) = 15%). The dry matter intake (DMI) (1.265 vs. 1.044 kg/day) and average daily gain (ADG) (0.176 vs. 0.143 kg/day) were higher (p < 0.05) in pelleted vs. conventional TMR-fed goats. Irrespective of the PF of the TMR, the 15% WS-fed animals had greater (p < 0.05) DMI (1.206 vs. 1.102 kg/day) and ADG (0.172 vs. 0.144 kg) when compared to those fed on 25% WS diets. Furthermore, feed-to-gain ratio (F:G) was higher (p < 0.05) in the 25% WS-fed goats when compared with the 15% WS-fed animals. Digestibility coefficients, nitrogen balancing, hepatic enzymes, blood metabolites, and hematological parameters were similar (p > 0.05) across all treatments. In conclusion, feeding pelleted TMR with WS improved DMI and growth performance as compared to those fed conventional TMR, and 15% WS performed better than 25% WS without exerting any adverse effects on blood metabolites, liver enzymes, or hematological parameters.

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Processing index of barley grain and dietary undigested neutral detergent fiber concentration affected chewing behavior, ruminal pH, and total tract nutrient digestibility of heifers fed a high-grain diet

Journal of Animal Science ◽

10.1093/jas/skab011 ◽

2021 ◽

Vol 99 (1) ◽

Author(s):

Tao Ran ◽

Atef M Saleem ◽

Karen A Beauchemin ◽

Gregory B Penner ◽

Wenzhu Yang

Keyword(s):

Barrier Function ◽

Latin Square ◽

Barley Grain ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Blood Metabolites ◽

Fiber Concentration ◽

Finishing Cattle ◽

Ruminal Ph ◽

Grain Processing

Abstract The objective of this study was to investigate the effects of processing index (PI) of barley grain and dietary undigested neutral detergent fiber (uNDF) concentration on dry matter (DM) intake, chewing activity, ruminal pH and fermentation characteristics, total tract digestibility, gastrointestinal barrier function, and blood metabolites of finishing beef heifers. The PI was measured as the density after processing expressed as a percentage of the density before processing, and a smaller PI equates to a more extensively processed. Six ruminally cannulated heifers (average body weight, 715 ± 29 kg) were used in a 6 × 6 Latin square design with three PI (65%, 75%, and 85%) × 2 uNDF concentration (low and high; 4.6% vs. 5.6% of DM) factorial arrangement. The heifers were fed ad libitum a total mixed ration consisting of 10% barley silage (low uNDF), or 5% silage and 5% straw (high uNDF), 87% dry-rolled barley grain, and 3% mineral and vitamin supplements. Interactions (P < 0.01) of PI × uNDF were observed for DM intake, ruminating and total chewing time, and DM digestibility in the total digestive tract. Intake of DM, organic matter (OM), starch, and crude protein (CP) did not differ (P > 0.14) between low and high uNDF diets, but intakes of NDF and acid detergent fiber were greater (P = 0.01) for high uNDF diets regardless of barley PI. Heifers fed high uNDF diets had longer (P = 0.05) eating times (min/d or min/kg DM) and tended (P = 0.10) to have longer total chewing times (min/kg DM) than those fed low uNDF diets. Additionally, heifers sorted (P = 0.01) against long particles (>19 mm) for high uNDF diets but not for low uNDF diets. Altering PI of barley grain did not affect (P > 0.12) total volatile fatty acid (VFA) concentration, molar percentages of individual VFA, or duration of ruminal pH < 5.8 and <5.6. Total VFA concentration was less (P = 0.01), acetate percentage was greater (P = 0.01), and duration of ruminal pH < 5.8 and <5.6 was less (P = 0.05) for high compared with low uNDF diets. Digestibility of DM, OM, and CP was greater (P = 0.02) for low vs. high uNDF diets with PI of 65% and 75%, with no difference between low and high uNDF diets at PI of 85%. Blood metabolites and gastrointestinal tract barrier function were not affected (P ≥ 0.10) by the treatments. These results suggest that increasing dietary uNDF concentration is an effective strategy to improve ruminal pH status in finishing cattle, regardless of the extent of grain processing, whereas manipulating the extent of barley processing did not reduce the risk of ruminal acidosis.

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Apparent total tract digestibility, ruminal fermentation, and blood metabolites in beef steers fed green-chopped cool-season forages

Journal of Animal Science ◽

10.1093/jas/skaa175 ◽

2020 ◽

Vol 98 (6) ◽

Author(s):

Tessa M Schulmeister ◽

Martin Ruiz-Moreno ◽

Mariana E Garcia-Ascolani ◽

Francine M Ciriaco ◽

Darren D Henry ◽

...

Keyword(s):

Lolium Multiflorum ◽

Nutrient Digestibility ◽

Neutral Detergent Fiber ◽

Blood Metabolites ◽

Beef Steers ◽

Annual Ryegrass ◽

Ruminal Fermentation ◽

Cool Season ◽

Plasma Urea ◽

Ruminal Ph

Abstract An experiment was conducted during the winter of two consecutive years to evaluate the effects of feeding green-chopped cool-season forages on digestibility, ruminal fermentation, and blood parameters in beef steers. Nine ruminally cannulated Angus crossbred steers (year 1: 359 ± 79 kg; year 2: 481 ± 105 kg) received ad libitum green-chopped forages from pastures planted with one of the following mixtures: 1) OAT = Horizon 201 oats (Avena sativa L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively; 2) RYE = FL401 cereal rye (Secale cereale L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 78 and 17 kg/ha, respectively; or 3) TRIT = Trical 342 triticale (X Triticosecale spp.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively. Intake was measured using the GrowSafe system and orts were discarded prior to subsequent feeding. After a 14-d adaptation, feed and fecal samples were collected twice daily for 4 d to determine apparent total tract nutrient digestibility using indigestible neutral detergent fiber (NDF) as an internal marker. On day 19, blood and ruminal fluid samples were collected every 3 h during a 24-h period to analyze plasma urea nitrogen (PUN) and glucose, ruminal pH, and concentration of ruminal ammonia nitrogen (NH3-N) and volatile fatty acids (VFA). Data were analyzed as a generalized randomized block design with repeated measures using the PROC MIX of SAS. No effect of treatment (P > 0.05) was observed for intake of dry matter, organic matter (OM), crude protein, NDF, or acid detergent fiber. Apparent total tract digestibility of nutrients was greater (P < 0.05) for OAT and TRIT when compared with RYE, with OM digestibility being 82.7%, 79.6%, and 69.5%, respectively. An effect of time (P < 0.01) was observed for ruminal pH. Plasma concentration of glucose was greater (P < 0.01) in steers consuming OAT, whereas steers fed RYE had greater (P < 0.05) concentrations of ruminal NH3-N and PUN, and the least concentration of total ruminal VFA (P < 0.05), despite having the greatest (P > 0.05) molar proportion of acetate, branched-chain VFA, and acetate:propionate. Increased nutrient digestibility and favorable ruminal fermentation and blood metabolites of OAT and TRIT are potentially conducive to enhanced growth performance when compared with RYE.

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Effects of Replacement of Wheat Straw with Corn Stover Based TMR on Growth Performance, Behavioural Characteristics, selected Blood Metabolites and Nutrient Digestibility in Beetal Bucks.

10.21203/rs.3.rs-1068651/v1 ◽

2021 ◽

Author(s):

Faisal Majeed ◽

Saeed Ahmed ◽

Muhammad Afzal Rashid ◽

Muhammad Qamer Shahid ◽

Imran Mohsin

Keyword(s):

Growth Performance ◽

Wheat Straw ◽

Corn Stover ◽

Average Daily Gain ◽

Experimental Period ◽

Nutrient Digestibility ◽

Blood Metabolites ◽

Treatment Groups ◽

Behavioural Characteristics ◽

Dietary Treatments

Abstract An experiment was executed to determine the effects of replacing wheat straw with corn stover on growth performance, behavioural characteristics, blood metabolites and nutrient digestibility in Beetal bucks. A total of twenty four Beetal bucks were assigned randomly to one of three treatment groups, having eight animals each, for 15 weeks experimental period. The dietary treatments included conventional (25% wheat straw and 75% concentrate), corn stover 50 (Fifty percent of wheat straw (12.5%) was replaced with corn stover, and corn stover 100 (Wheat straw was completely replaced with corn stover). Dietary replacement of wheat straw with corn stover has resulted in an increased dry matter intake and average daily gain by 10 and 26%, respectively in the bucks. Rumen pH and fecal score, however, remained unaffected by dietary replacement of wheat straw with corn stover in the bucks. The replacement of wheat straw with corn stover has resulted in an increased feeding and rumination time, improved lying time and length and decreased number of bouts in the bucks. Blood glucose, urea, bilirubin and calcium levels were remained unaffected by replacing wheat straw with corn stover. The blood phosphorous level, however, was decreased in bucks fed corn stover based ration. The bucks fed corn stover based ration has resulted in an increased digestibility of nutrients including organic matter, crude protein, neutral and acid detergent fiber compared to those fed wheat straw based total mixed ration.

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