129 Genomic Relationship Between PRRSV Wild-type Infection and PRRSV Vaccination for Antibody Response and Reproductive Performance

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 18-18
Author(s):  
Leticia P Sanglard ◽  
Felipe Hickmann ◽  
Yijian Huang ◽  
Kent A Gray ◽  
Daniel Linhares ◽  
...  
Keyword(s):  
Antibody Response ◽  
Reproductive Performance ◽  
Genetic Correlations ◽  
Clinical Signs ◽  
Respiratory Syndrome Virus ◽  
Wild Type ◽  
Large White ◽  
Selection For ◽  
Indicator Trait ◽  
Type Infection

Abstract Immunoglobulin G antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) has been proposed as an indicator trait for improved reproductive performance in PRRSV-infected purebred sows and PRRSV-vaccinated crossbred gilts. In this study, we investigated the genetic correlations (rg) of S/P ratio following a PRRSV outbreak and PRRSV-vaccination with performance in non-exposed and PRRSV-exposed sows. PRRSV outbreak phase was defined based on previously described methodologies after the detection of typical clinical signs of PRRSV infection. 541 Landrace sows had S/P ratio measured at ~54 days after the beginning of the PRRSV outbreak (S/Poutbreak), and 906 Landrace x Large White naïve F1 gilts had S/P ratio measured at ~50 days after vaccination with a commercial modified live PRRSV vaccine (S/PVx). 711 and 428 Landrace sows had reproductive performance recorded before and during the PRRSV outbreak, respectively. 811 vaccinated F1 animals had farrowing performance for up to 3 parities. All animals were genotyped for ~28K SNPs. The estimate of rg of S/Poutbreakwith S/PVx was high (rg±SE = 0.72±0.18). Estimates of rg of S/Poutbreak with reproductive performance in F1 sows were low to moderate, ranging from 0.05±0.23 (number stillborn) to 0.30±0.20 (total number born). Estimates of rg of S/PVxwith reproductive performance in non-infected purebred sows were moderate and favorable with number born alive (0.50±0.23), but low (0 to -0.11±0.23) with litter mortality traits. Estimates of rg of S/PVx were moderate and negative (-0.47±0.18) with the number of mummies in PRRSV-infected purebred sows and low with other traits (-0.29±0.18 for total number born to 0.05±0.18 for number stillborn). These results indicate that selection for antibody response following a PRRSV outbreak collected in purebred sows and to PRRSV vaccination collected in commercial crossbred gilts may increase litter size of non-infected and PRRSV-exposed purebred and commercial crossbred sows.

127 Antibody Response as an Indicator Trait for Improved Reproductive Performance During a PRRSV Outbreak: Genetic Correlations of S/P Ratio with Reproductive Traits in Landrace and Duroc Sows

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 19-20
Author(s):  
Felipe Hickmann ◽  
José Braccini Neto ◽  
Luke M Kramer ◽  
Kent A Gray ◽  
Yijian Huang ◽  
...  
Keyword(s):  
Antibody Response ◽  
Reproductive Performance ◽  
Genetic Correlations ◽  
Reproductive Traits ◽  
Respiratory Syndrome Virus ◽  
Potential Indicator ◽  
Host Genetic ◽  
Heritability Estimates ◽  
Terminal Sire ◽  
Indicator Trait

Abstract Previous studies proposed the use of antibody response to Porcine Reproductive and Respiratory Syndrome virus (PRRSV), measured as sample-to-positive (S/P) ratio, as a potential indicator trait to improve the reproductive performance of PRRSV-infected Landrace sows. However, this indicator trait has not yet been validated in Landrace sows or evaluated in a terminal sire line, such as Duroc. The main objective of this work was to perform host-genetic analyses of S/P ratio and reproductive traits during a PRRSV outbreak in maternal and terminal breeds. The data consisted of 690 Duroc and 541 Landrace multiparous sows (1.9±1.2 and 2.3±1.5, respectively) with S/P ratio collected at approximately 54 days after the predicted beginning of the outbreak. Of these, 644 Duroc and 528 Landrace sows also had reproductive data recorded during the PRRSV outbreak comprising number of piglets born alive (NBA), stillborn piglets (NSB), mummified piglets (NBM), number born dead (NBD; sum of NSB and NBM), total number born (TNB; sum of NBA and NBD), and number weaned (NW). All animals had genotype data on ~30K SNPs common across both breeds. Heritability estimates (± standard error) of S/P ratio during the PRRSV outbreak were moderate, with 0.33±0.06 for Duroc and 0.28±0.07 for Landrace. Reproductive traits during the PRRSV outbreak had overall low heritability estimates (≤0.18). Favorable genetic correlations of S/P ratio with NBA (0.65±0.33), in accordance with previous studies, and NBD (-0.33±0.28) were observed for Landrace sows only. Estimates of genetic correlation with other traits were -0.21±0.30 (NBM), -0.12±0.29 (NSB), 0.10±0.38 (NW), and 0.54±1.29 (TNB) for Landrace. For Duroc, these estimates were weaker: -0.33±0.40 (NBA), 0.26±0.27 (NBA), and 0.28±0.30 (NW), with convergence issues for mortality traits. These results further support the use of S/P ratio as an indicator trait for improved reproductive performance during a PRRSV outbreak in Landrace sows.

scholarly journals 27 Genomic relationship between antibody response to porcine reproductive and respiratory syndrome virus vaccination and reproductive performance in commercial sows

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 20-21
Author(s):  
Leticia P Sanglard ◽  
Rohan Fernando ◽  
Kent A Gray ◽  
Daniel C Linhares ◽  
Jack C Dekkers ◽  
...  
Keyword(s):  
Antibody Response ◽  
Reproductive Performance ◽  
Association Studies ◽  
Genetic Correlations ◽  
Reproductive Traits ◽  
Respiratory Syndrome Virus ◽  
Genome Wide Association Studies ◽  
Prrs Virus ◽  
Positive Ratio ◽  
Commercial Farms

Abstract Antibody response, measured as sample-to-positive ratio (S/P), to porcine reproductive and respiratory syndrome (PRRS) after PRRS outbreaks has been proposed as an indicator trait to improve reproductive performance in PRRS-infected sows. However, waiting for a PRRS outbreak to occur and having different relationships in healthy pigs may limit the use of this trait. Thus, we proposed to investigate if this relationship also occurs between S/P to PRRS vaccination and reproductive performance in sows without exposure to PRRS. Nine hundred six F1 replacement gilts (139□17 days old) from two commercial farms were vaccinated with a commercial modified live PRRS virus vaccine. Blood samples were collected at 52 days after vaccination to measure S/P to PRRS and SNP genotyping. Reproductive performance included: number born alive (NBA), number of piglets weaned, number born mummified (MUM), number stillborn (NSB), and pre-weaning mortality (PWM) at parities (P) 1 to 3. Average performance was calculated for each trait per sow per year (PSY). Farrowing rate and age at first service were also analyzed. BayesC0 was used to estimate genetic correlations between S/P and reproductive performance. Bivariate genome-wide association studies of antibody response and reproductive traits were performed using BayesB. High genetic correlations between S/P and farrowing performance were identified for NBA_P1 (0.61±0.16), PWM_P2 (-0.64±0.15), PWM_P2 (-0.63±0.20), NSB_P3 (-0.84±0.05), MUM_P3 (-0.83±0.11), and NSB_PSY (-0.90±0.05). A QTL was identified on chromosome 7 (MHC region) for these reproductive traits and for S/P, explaining from 1.2% (PWM_P2) to 22.4% (S/P) of the genetic variance. SNP H3GA0020505 explained most of the variance in this region for these traits. Heterozygote animals for this SNP had overall better performance: greater S/P (P = 0.001), greater (P = 0.06) NBA_P1, and lesser (P = 0.06) MUM_P3 than other genotypes. These results suggest that antibody response to PRRS vaccination may be used as a genetic indicator to improve reproductive performance in commercial pigs.

scholarly journals Host Genetics of Response to Porcine Reproductive and Respiratory Syndrome in Sows: Antibody Response as an Indicator Trait for Improved Reproductive Performance

2021 ◽  
Vol 12 ◽  
Author(s):  
Felipe M. W. Hickmann ◽  
José Braccini Neto ◽  
Luke M. Kramer ◽  
Yijian Huang ◽  
Kent A. Gray ◽  
...  
Keyword(s):  
Antibody Response ◽  
Reproductive Performance ◽  
Association Studies ◽  
Genetic Correlations ◽  
Genome Wide Association Studies ◽  
Nucleotide Polymorphisms ◽  
Ratio Measurement ◽  
Prrs Virus ◽  
Trait Locus ◽  
Indicator Trait

Antibody response to porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) infection, measured as sample-to-positive (S/P) ratio, has been proposed as an indicator trait for improved reproductive performance during a PRRS outbreak in Landrace sows. However, this result has not yet been validated in Landrace sows or evaluated in terminal sire lines. The main objectives of this work were to validate the use of S/P ratio as an indicator trait to select pigs during a PRRS outbreak and to explore the genetic basis of antibody response to PRRSV. Farrowing data included 2,546 and 2,522 litters from 894 Duroc and 813 Landrace sows, respectively, split into pre-PRRS, PRRS, and post-PRRS phases. Blood samples were taken from 1,231 purebred sows (541 Landrace and 690 Duroc) following a PRRS outbreak for subsequent PRRSV ELISA analysis for S/P ratio measurement. All animals had high-density genotype data available (29,799 single nucleotide polymorphisms; SNPs). Genetic parameters and genome-wide association studies (GWAS) for S/P ratio were performed for each breed separately. Heritability estimates (± standard error) of S/P ratio during the PRRS outbreak were moderate, with 0.35 ± 0.08 for Duroc and 0.34 ± 0.09 for Landrace. During the PRRS outbreak, favorable genetic correlations of S/P ratio with the number of piglets born alive (0.61 ± 0.34), number of piglets born dead (−0.33 ± 0.32), and number of stillborn piglets (−0.27 ± 0.31) were observed for Landrace sows. For Duroc, the GWAS identified a major quantitative trait locus (QTL) on chromosome (Chr) 7 (24-15 megabases; Mb) explaining 15% of the total genetic variance accounted for by markers (TGVM), and another one on Chr 8 (25 Mb) explaining 2.4% of TGVM. For Landrace, QTL on Chr 7 (24–25 Mb) and Chr 7 (108–109 Mb), explaining 31% and 2.2% of TGVM, respectively, were identified. Some of the SNPs identified in these regions for S/P ratio were associated with reproductive performance but not during the PRRS outbreak. Genomic prediction accuracies for S/P ratio were moderate to high for the within-breed analysis. For the between-breed analysis, these were overall low. These results further support the use of S/P ratio as an indicator trait for improved reproductive performance during a PRRS outbreak in Landrace sows.

scholarly journals Genomics of response to PRRSV in purebred and crossbred sows: antibody response and performance following natural infection versus vaccination

2021 ◽  
Author(s):  
Leticia P Sanglard ◽  
Felipe M W Hickmann ◽  
Yijian Huang ◽  
Kent A Gray ◽  
Daniel C L Linhares ◽  
...  
Keyword(s):  
Antibody Response ◽  
Genetic Correlation ◽  
Reproductive Performance ◽  
Genetic Relationships ◽  
Natural Infection ◽  
Respiratory Syndrome Virus ◽  
Large White ◽  
And Performance ◽  
Genomic Regions ◽  
Indicator Traits

Abstract Antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) following a PRRSV-outbreak (S/POutbreak) in a purebred nucleus and following a PRRSV-vaccination (S/PVx) in commercial crossbred herds have been proposed as genetic indicator traits for improved reproductive performance in PRRSV-infected purebred and PRRSV-vaccinated crossbred sows, respectively. In this study, we investigated the genetic relationships of S/POutbreak and S/PVx with performance at the commercial (vaccinated crossbred sows) and nucleus level (non-infected and PRRSV-infected purebred sows), respectively, and tested the effect of previously identified SNP for these indicator traits. Antibody response was measured on 541 Landrace sows approximately 54 days after the start of a PRRSV outbreak, and on 906 F1 (Landrace x Large White) gilts approximately 50 days after vaccination with a commercial PRRSV vaccine. Reproductive performance was recorded for 711 and 428 Landrace sows before and during the PRRSV outbreak, respectively, and for 811 vaccinated F1 animals. The estimate of the genetic correlation (rg) of S/POutbreak with S/PVx was 0.72±0.18. The estimates of rg of S/POutbreak with reproductive performance in vaccinated crossbred sows were low to moderate, ranging from 0.05±0.23 to 0.30±0.20. The estimate of rg of S/PVx with reproductive performance in non-infected purebred sows was moderate and favorable with number born alive (0.50±0.23) but low (0±0.23 to -0.11±0.23) with piglet mortality traits. The estimates of rg of S/PVx were moderate and negative (-0.38±0.21) with number of mummies in PRRSV-infected purebred sows and low with other traits (-0.30±0.18 to 0.05±0.18). Several significant associations (P0 > 0.90) of previously reported SNP for S/P ratio (ASGA0032063 and H3GA0020505) were identified for S/P ratio and performance in non-infected purebred and PRRSV-exposed purebred and crossbred sows. Genomic regions harboring the major histocompatibility complex class II region significantly contributed to the genetic correlation of antibody response to PRRSV with most of the traits analyzed. These results indicate that selection for antibody response in purebred sows following a PRRSV outbreak in the nucleus and for antibody response to PRRSV vaccination measured in commercial crossbred sows are expected to increase litter size in purebred and commercial sows.

scholarly journals Host Genetics of Response to Porcine Reproductive and Respiratory Syndrome in Sows: Reproductive Performance

2021 ◽  
Vol 12 ◽  
Author(s):  
Felipe M. W. Hickmann ◽  
José Braccini Neto ◽  
Luke M. Kramer ◽  
Yijian Huang ◽  
Kent A. Gray ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Additive Genetic Variance ◽  
Genetic Correlations ◽  
Reproductive Traits ◽  
The Other ◽  
Nucleotide Polymorphisms ◽  
Host Genetics ◽  
Other Hand ◽  
Selection For ◽  
Heritability Estimates

Porcine Reproductive and Respiratory Syndrome (PRRS) is historically the most economically important swine disease worldwide that severely affects the reproductive performance of sows. However, little is still known about the genetic basis of reproductive performance in purebred herds during a PRRS outbreak through the comparison of maternal and terminal breeds. Thus, the objective of this work was to explore the host genetics of response to PRRS in purebred sows from two breeds. Reproductive data included 2546 Duroc and 2522 Landrace litters from 894 and 813 purebred sows, respectively, which had high-density genotype data available (29,799 single nucleotide polymorphisms; SNPs). The data were split into pre-PRRS, PRRS, and post-PRRS phases based on standardized farrow-year-week estimates. Heritability estimates for reproductive traits were low to moderate (≤0.20) for Duroc and Landrace across PRRS phases. On the other hand, genetic correlations of reproductive traits between PRRS phases were overall moderate to high for both breeds. Several associations between MARC0034894, a candidate SNP for response to PRRS, with reproductive performance were identified (P-value < 0.05). Genomic analyses detected few QTL for reproductive performance across all phases, most explaining a small percentage of the additive genetic variance (≤8.2%, averaging 2.1%), indicating that these traits are highly polygenic. None of the identified QTL within a breed and trait overlapped between PRRS phases. Overall, our results indicate that Duroc sows are phenotypically more resilient to PRRS than Landrace sows, with a similar return to PRRS-free performance between breeds for most reproductive traits. Genomic prediction results indicate that genomic selection for improved reproductive performance under a PRRS outbreak is possible, especially in Landrace sows, by training markers using data from PRRS-challenged sows. On the other hand, the high genetic correlations with reproductive traits between PRRS phases suggest that selection for improved reproductive performance in a clean environment could improve performance during PRRS, but with limited efficiency due to their low heritability estimates. Thus, we hypothesize that an indicator trait that could be indirectly selected to increase the response to selection for these traits would be desirable and would also improve the reproductive performance of sows during a PRRS outbreak.

scholarly journals Resilience Effects of SGK1 and TAP1 DNA Markers during PRRSV Outbreaks in Reproductive Sows

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 902
Author(s):  
Marina Laplana ◽  
Joan Estany ◽  
Lorenzo José Fraile ◽  
Ramona Natacha Pena
Keyword(s):  
Candidate Genes ◽  
Reproductive Performance ◽  
Dna Markers ◽  
Health Problems ◽  
Respiratory Syndrome Virus ◽  
Large White ◽  
Genome Wide ◽  
Stable Performance ◽  
Potential Use

The porcine reproductive and respiratory syndrome virus (PRRSV) is a major infectious stressor that causes serious health problems and productivity drops. Based on previous genome-wide analyses, we selected SGK1 and TAP1 as candidate genes for resilience, and genotyped three mutations, including a 3′UTR variant SGK1_rs338508371 and two synonymous variants TAP1_rs1109026889 and TAP1_rs80928141 in 305 Landrace × Large White sows. All polymorphisms affected the reproductive performance in the outbreak, but not during the endemic phase, thereby indicating a potential use of these markers for resilience. Moreover, some genotypes were associated with a stable performance across PRRSV phases. Thus, in the outbreak, the SGK1_rs338508371 AA sows had less piglets born alive (p < 0.0001) and more stillborns (p < 0.05) while other sows were able to keep their productivity. During the outbreak, TAP1_rs80928141 GG sows had less piglets born alive (p < 0.05) and both TAP1 polymorphisms influenced the number of mummies in an additive manner (p < 0.05). Remarkably, TAP1_rs80928141 AA sows had around one mummy more than GG sows (p < 0.01). Resilience to PRRSV could be improved by including the SGK1 and TAP1 markers in crossbreeding and/or selection schemes, as they contribute to maintaining a stable number of piglets born alive and lost, particularly mummies, despite the outbreak.

Effect of selection for leanness on overall reproductive performance in Large White sows

1995 ◽  
Vol 61 (3) ◽  
pp. 561-564 ◽  
Author(s):  
J. B. Gaughan ◽  
R. D. A. Cameron ◽  
G. McL. Dryden ◽  
M. J. Josey
Keyword(s):  
Birth Weight ◽  
Reproductive Performance ◽  
Live Weight ◽  
The Other ◽  
Large White ◽  
Weaning Weight ◽  
Weaned Piglet ◽  
Selection For ◽  
Weight Number ◽  
M 14

AbstractReproductive records from 1072 Large White sows (3589 litters) were used to examine the effect ofbackfat depth (Bd) and live weight (Lw) at selection on first litter and lifetime reproductive performance. The variables investigated included mating age, total piglets born, total born alive, piglet birth weight, number weaned, piglet weaning weight, weaning to remating period and number of litters produced. Using backfat depth, sows were categorized into three groups: L, 9 to 13 mm; M, 14 to 16 mm; and F, ≥17 mm. Bd had no significant effect (P > 0·05) on the measured traits for the sows grouped by fat at first parity. When lifetime reproductive data were analysed, the L group had fewer litters (P < 0·05), and weaned fewer pigs (P < 0·01) than either the M or F group. The birth weight for piglets from L sows were significantly higher (P < 0·05) than for piglets from the other groups. The data suggest that the reproductive performance of L sows is not as good as that of M or F sows.

scholarly journals Effects of dietary arginine supplementation on reproductive performance and immunity of sows

2013 ◽  
Vol 58 (No. 4) ◽  
pp. 167-175 ◽  
Author(s):  
L. Che ◽  
P. Yang ◽  
Z. Fang ◽  
Y. Lin ◽  
D. Wu
Keyword(s):  
Immune Response ◽  
Fetal Growth ◽  
Reproductive Performance ◽  
Control Diet ◽  
Late Gestation ◽  
Respiratory Syndrome Virus ◽  
Control Group ◽  
Placental Development ◽  
Large White ◽  
Immunological Parameters

Arginine (Arg) is considered to have beneficial effects on placental development and function, as well as reproductive performance. The well-developed placenta is highly required in late gestation for rapid fetal growth, however, it is unknown if there is a crucial role of Arg in late gestation. Likewise, the immunological response of sows to Arg needs to be determined. Therefore, this study is designed to investigate the effects of dietary Arg supplementation on reproductive performance and immunity of sows. At day 30 of gestation, sixty sows (Landrace &times; Large White) were allocated to 3 groups receiving corn and soybean-based control diet (control group, n = 20), control diet supplemented with 1% l-arginine HCl until day 90 of gestation (Arg90 group, n = 20), and control diet supplemented with 1% l-arginine HCl until day 114 of gestation (Arg114 group, n = 20), respectively. Litter performance was recorded at parturition. Blood samples (n = 6) collected at days 30, 90, and 110 of gestation were measured for metabolic and immunological parameters. At parturition, total litter size was not affected by dietary Arg supplementation. As a result of less pigs born dead, however, sows in Arg114 group had more pigs born alive than sows in control group (+1.6 pigs, P &lt; 0.05), total and live litter weights were increased (+1.6~2.1 kg, P &lt; 0.05) in Arg114 group relative to both control and Arg90 groups. Compared with control group, dietary Arg supplementation increased (+12~110%, P &lt; 0.05) plasma levels of ornithine, proline, and arginine at either day 90 (Arg90 and Arg110 groups) or day 110 of gestation (Arg110 group). Moreover, immune response was enhanced in Arg-supplemented sows, as indicated by the increased levels of serum immunoglobulin and porcine reproductive and respiratory syndrome virus (PRRSV) antibody. These findings indicate dietary Arg supplementation can improve litter performance and immune response, and the beneficial effect of Arg on fetal growth is evident in late gestation.

scholarly journals Genetic Analysis of Feet and Leg Conformation and Proportion of Crushed Piglets in Austrian Large White and Landrace Sows

2019 ◽  
Vol 67 (5) ◽  
pp. 1213-1219
Author(s):  
Christina Pfeiffer ◽  
Birgit Fuerst-Waltl ◽  
Katharina Schodl ◽  
Peter Knapp
Keyword(s):  
Mortality Rate ◽  
Animal Welfare ◽  
Genetic Correlations ◽  
Large White ◽  
Breeding Values ◽  
Piglet Mortality ◽  
Selection For ◽  
Estimated Breeding Values ◽  
Highly Correlated ◽  
Almost All

Selection for feet and leg traits can increase longevity as well as several performance traits in sows. Moreover, good feet and leg quality contributes improving animal welfare not only for sows, but also for piglets. Sows with feet and leg problems have a higher risk of crushing their piglets when lying down. Therefore, a feet and leg scoring scheme was derived and applied by trained assessors. Furthermore, piglet mortality rate by crushing was recorded. Genetic parameters as well as breeding values were estimated and the effect of the traits on piglet mortality rate by crushing was investigated. Data of 993 Large White and 299 Landrace sows of 23 nucleus farms were analyzed. Heritabilities ranged from 0.03 to 0.16 for piglet mortality rate by crushing and pastern fore legs. Some genetic correlations between feet and leg traits were significantly highly correlated (0.69 to 0.79). Sows with favorable scores for almost all feet and leg scores had higher estimated breeding values for piglet mortality rate. Higher values indicate less crushed piglets and are thus favorable. An introduction of a feet and leg scoring scheme into routine genetic evaluation may thus contribute to animal welfare improvement for sows and piglets.

The effect of age of purebred large white and landrace boars at time of service on herd performance

1990 ◽  
Vol 1990 ◽  
pp. 59-59
Author(s):  
M. J. H. Francis ◽  
J. T. Mercer
Keyword(s):  
Reproductive Performance ◽  
Breeding Performance ◽  
Large White ◽  
The Past ◽  
Selection For ◽  
The Relationship ◽  
Effect Of Age

Practically, the effect of selection for growth and efficiency on breeding performance has been largely ignored in the past, despite changes in the age and weight of animals at puberty. It has been demonstrated that it is beneficial to delay service in gilts, both to improve their condition and allow them to complete more oestrous cycles prior to service. It seems likely that performance of boars might also be enhanced if they were worked later.This analysis was designed to examine the relationship between age and reproductive performance in the boar, with a view to developing guidelines for young boar management.

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