163 Relative Bioavailability of Bis-glycinate Bound Copper in Beef Steers Fed a High Antagonist Growing Diet

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 121-122
Author(s):  
Erin L Deters ◽  
Allison VanDerWal ◽  
Katherine VanValin ◽  
Stephanie L Hansen
Keyword(s):  
Body Weight ◽  
Repeated Measures ◽  
Dry Matter ◽  
Random Effect ◽  
Relative Bioavailability ◽  
Fixed Effect ◽  
Experimental Unit ◽  
Beef Steers ◽  
Lower Solubility ◽  
High Concentrations

Abstract To assess relative bioavailability of an organic Cu source, 90 Angus-cross steers (265 ± 21 kg) were blocked by body weight to pens with GrowSafe bunks and randomly assigned to dietary Cu treatments (14–18 steers/treatment): 0 mg Cu/kg dry matter (DM; CON), 5 or 10 mg Cu/kg DM as Cu sulfate (CS5; CS10) or chelated bis-glycinate Cu (GLY5; GLY10). Steers were fed a common high antagonist growing diet (0.48% S; 5.3 mg Mo/kg DM; 4.9 mg Cu/kg DM). Blood was collected from all steers on d 0, 28, 56, 84 and 124. Liver samples were collected at trial beginning (d -3/-2) and end (d 125/126). Data were analyzed using ProcMixed of SAS (experimental unit = steer; fixed effect = treatment; random effect = block). Plasma Cu was analyzed as repeated measures (repeated effect = day). Plasma and liver Cu concentrations were regressed against Cu intake using ProcGLM to calculate relative bioavailability of GLY. Initial liver Cu did not differ between treatments (P ≥ 0.63), but final liver Cu was lesser in CON versus steers supplemented 5 or 10 mg Cu/kg DM from either source (P ≤ 0.09). Final liver Cu was greater for CS versus GLY (P < 0.01). Plasma Cu for all treatments decreased through d 28; final plasma Cu was greatest for steers supplemented either source at 10 mg Cu/kg DM (treatment × day P < 0.01). Relative bioavailability of GLY was 82% compared to CS (P < 0.01) based on liver Cu but did not differ based on plasma Cu (P = 0.60). High concentrations of dietary antagonists and lower solubility of GLY (68.9% relative to CS) in rumen-like conditions (pH 5.2) may have resulted in free thiomolybdate absorption across the rumen wall and subsequent depletion of liver Cu stores in GLY supplemented steers.

303 Effects of Supplementing Plexomin Zn™ on Post-transit Growth Performance and Plasma Zn and Serum Lactate Concentrations of Beef Steers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 163-163
Author(s):  
Katie J Heiderscheit ◽  
Stephanie L Hansen
Keyword(s):  
Body Weight ◽  
North America ◽  
Repeated Measures ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Serum Lactate ◽  
Dry Matter Intake ◽  
Experimental Unit ◽  
Beef Steers ◽  
Daily Gain

Abstract To determine effects of supplemental Zn on post-transit growth and plasma Zn and serum lactate concentrations, 54 Angus-cross steers (297 kg ± 12) were stratified by body weight to nine GrowSafe-equipped pens. Treatments began 25 d prior to transit and included: control (CON; no supplemental Zn), industry (IND; 70 mg supplemental Zn/kg DM), and supranutritional Zn (SUPZN; 120 mg supplemental Zn/kg DM). Supplemental Zn was provided as bis-glycinate bound Zn (Plexomin Zn; Phytobiotics North America, Cary, NC). On d 0, steers were loaded into a commercial trailer and transported 18 h (1,822 km). Individual BW were recorded on d -26, -25, -1, 0 (pre-transit), 1 (post-transit), 6, 27, and 28. Blood was collected on d -1, 1, 6, and 28. Data were analyzed using Proc Mixed of SAS with fixed effect of diet and steer as the experimental unit (n = 18 steers per treatment). Blood measures were analyzed as repeated measures with the repeated effect of day. Percent of pre-transit dry matter intake (DMI) was calculated relative to DMI for the 5 days prior to transit. Plasma Zn concentrations were greater in IND and SUPZN than CON (P = 0.01) and were greater on d 1 than d -1, 6, and 27 (P < 0.01). By d 2, both IND and SUPZN returned to pre-transit DMI levels while CON did not (P = 0.01). Further, DMI from d 1 to 28 was greatest in IND, intermediate in SUPZN, and least in CON (P = 0.04). Average daily gain from d 6 to 28 linearly improved with increasing Zn supplementation (P = 0.02). Regardless of treatment, serum lactate concentrations were greater post-transit compared to pre-transit values (P = 0.02). Steers supplemented dietary Zn recovered DMI faster and had greater ADG after a transit event.

scholarly journals Relative bioavailability of organic bis-glycinate bound copper relative to inorganic copper sulfate in beef steers fed a high antagonist growing diet

2021 ◽  
Author(s):  
Erin L Deters ◽  
Allison J VanDerWal ◽  
Katherine R VanValin ◽  
Stephanie L Hansen
Keyword(s):  
Repeated Measures ◽  
Average Daily Gain ◽  
Relative Bioavailability ◽  
Feedlot Cattle ◽  
Experimental Unit ◽  
Beef Steers ◽  
High Concentration ◽  
Ruminal Ph ◽  
Lower Solubility ◽  
Dietary Treatments

Abstract To assess the relative bioavailability of bis-glycinate bound Cu, 90 Angus-cross steers (265 ± 21 kg) were blocked by body weight (BW) to pens with GrowSafe bunks and randomly assigned to dietary treatments (14-18 steers/treatment): 0 mg supplemental Cu/kg dry matter (DM; CON), 5 or 10 mg supplemental Cu/kg DM as Cu sulfate (CS5; CS10) or bis-glycinate bound Cu (GLY5; GLY10). Steers received a high antagonist growing diet (analyzed 4.9 mg Cu/kg DM, 0.48% S, and 5.3 mg Mo/kg DM). Steers were weighed at the beginning (d -1 and 0) and end (d 125 and 126) of the trial to determine average daily gain (ADG) and gain:feed (G:F). Blood was collected from all steers on d 0, 28, 56, 84, and 126. Liver samples were collected on d -3 or -2 and d 123 or 124. Data were analyzed using ProcMixed of SAS (experimental unit = steer; fixed effect = treatment and block). Plasma Cu was analyzed as repeated measures (repeated effect = day). Plasma and liver Cu concentrations were regressed against total Cu intake using ProcGLM to calculate relative bioavailability of GLY. Final BW and overall ADG were greatest for CS5 and CS10 and least for CON and GLY5 (P = 0.01). Overall DMI was not affected by treatment (P = 0.14), but overall G:F tended to be greatest for CS5, CS10, and GLY5 and least for CON (P = 0.08). Total and supplemental Cu intake was greatest for steers supplemented either source at 10 mg Cu/kg DM and least for CON (P < 0.01). However, total and supplemental Cu intake was greater for CS5 than GLY5 (P < 0.01). Final liver Cu concentrations were greatest for CS10, least for CON, CS5, and CS10, and intermediate for GLY10 (P < 0.01). Final plasma Cu was greatest for steers supplemented either source at 10 mg Cu/kg DM (P < 0.01). Relative bioavailability of GLY was 82% compared to CS based on liver Cu (P < 0.01) but did not differ based on plasma Cu (P = 0.60). The lesser bioavailability of GLY relative to CS could be due to a high concentration of dietary antagonists and lower solubility of GLY (68.9% relative to CS) in pH conditions (5.2) similar to the ruminal pH of a beef animal consuming a high concentrate diet. Future studies should examine the effects of bis-glycinate bound Cu fed in blended combination with inorganic Cu sulfate to determine the most effective blend of sources for feedlot cattle experiencing varying amounts of dietary Cu antagonists.

305 Methane Emissions and Apparent Total Tract Nutrient Digestibility of Feedlot Beef Steers Under Intensive Management with or Without an Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  
Keyword(s):  
Treatment Period ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Methane Emissions ◽  
Nutrient Digestibility ◽  
Experimental Unit ◽  
Beef Steers ◽  
Ch4 Production

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.

scholarly journals 230 Effects of encapsulated calcium-ammonium nitrate on performance and methane production of beef steers grazing mixed winter forage

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio Doblado ◽  
Sebastian E Mejia-Turcios ◽  
Miranda K Stotz ◽  
David Vargas ◽  
Rafael Canonenco de Araujo ◽  
...  
Keyword(s):  
Ammonium Nitrate ◽  
Methane Production ◽  
Repeated Measures ◽  
Block Design ◽  
Random Effect ◽  
Experimental Unit ◽  
Beef Steers ◽  
Adaptation Period ◽  
Calcium Ammonium Nitrate ◽  
Supplement Intake

Abstract The effects of encapsulated calcium-ammonium nitrate (eCAN) on in vivo methane production and performance were evaluated. A generalized randomized block design was used with beef steers grazing a mixed winter forage (Triticum aestivum, Triticosecale rimpaui, and Secale cereale) for 49 d. Thirty-six Angus-crossbred steers (332 ± 53 kg) were blocked by BW and randomly assigned to 1 of 3 treatments: 1) winter pasture + corn (WC), 2) WC + 328 mg/kg of BW eCAN (WCN) and 3) WC + 124 mg/kg of BW UREA (WCU), all supplemented with corn (0.3% BW treatments). Treatments WCN and WCU were isonitrogenous. A 14d adaptation period was used to adapt cattle to treatments. Methane emissions were measured using the sulfur hexafluoride tracer technique. Blood samples and BW were taken at d 0, 24, 35, and 49. Supplemental corn and NPN orts were collected daily. Data were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effect of pasture (block); BUN, supplement intake and CP intake were analyzed with repeated measures. Steer was considered the experimental unit. Methane production was not different (P > 0.05) considering g/d, g/kg of BW, g/kg of MBW, or g/kg of ADG. Treatments did not affect ADG (P = 0.941). Supplement intake was affected by treatment (P < 0.001), with WC (0.979 kg) being greater compared to WCU (0.887 kg) and WCN (0.706 kg). Total CP intake increased (P < 0.001) with WCU (0.155 kg) and WCN (0.116 kg), compared to WC (0.074 kg), which did not have a non-protein source. Blood urea nitrogen was affected by day, with d 24 (18.598 mg/dL) being greater compared to d 0 (8.215 mg/dL), 35 (10.549 mg/dL) and 49 (14.5744 mg/dL). The eCAN did not effectively replace urea as a NPN source to mitigate enteric methane.

scholarly journals 191 Effects of injectable vitamin C at weaning and prior to transit on growth performance and immune function of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 152-153
Author(s):  
Aubree M Beenken ◽  
Erin L Deters ◽  
Colten W Dornbach ◽  
Stephanie L Hansen ◽  
Joshua C McCann ◽  
...  
Keyword(s):  
Vitamin C ◽  
Growth Performance ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Average Daily Gain ◽  
Experimental Unit ◽  
Beef Steers ◽  
Diarrhea Virus ◽  
Plasma Ascorbate ◽  
Antibody Titers

Abstract Ninety-one early-weaned (65 ± 11 d) Angus steers (92 ± 4 kg) were blocked by age to a 2 × 2 factorial examining effects of injectable vitamin C (VC) at weaning and/or prior to transport to the feedlot on antibody titers and growth performance. Injections (20 mL/steer) of VC (250 mg sodium ascorbate/mL) or saline (SAL) were given at time of weaning on d 0 (WEAN) and/or prior to a 6 hr trucking event to a feedlot on d 49 (TRANS). Steers were given booster vaccinations on d 0. Steers were weighed on d 0, 1, 14, 48, 49, 64, 106, and 107. Blood was collected (12 steers/treatment) on d 0, 1, 2, 14, 49 (pre- and post-transit), 50, and 51. Data were analyzed via Proc-Mixed of SAS (experimental unit = steer; n = 22–23/treatment) with fixed effects of block, WEAN, TRANS, and WEAN × TRANS. Plasma ascorbate concentrations for weaning (d 0, 1, and 2) and transit (d 49-pre-trucking, 49-post-trucking, 50, and 51) were analyzed as repeated measures (repeated effect = day). Plasma ascorbate concentrations were greater on d 1 and 2 for steers that received VC at weaning (VC = 19.6, SAL = 8.8 ± 1.26 µM; WEAN × day P < 0.01). Similarly, ascorbate concentrations were greater on d 49 post-trucking, 50, and 51 for steers that received VC pre-transit (TRANS × day P = 0.01). Treatments did not affect bodyweight or average daily gain throughout the trial (P > 0.32). There were no effects of treatment on serum Bovine Viral Diarrhea Virus type 2 antibody titers on d 14 or 51 (P > 0.33). An injection of VC administered to early weaned beef steers at weaning or pre-transit increases plasma ascorbate concentrations but does not improve growth performance or antibody response to vaccination booster.

Effects of roughage type on particle separation, rumination, fiber mat characteristics, in situ degradation, and ruminal fermentation parameters in beef steers

2021 ◽  
Author(s):  
Catherine L Lockard ◽  
Caleb G Lockard ◽  
Wyatt N Smith ◽  
Kendall J Karr ◽  
Ben P Holland ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Fluid Collection ◽  
Latin Square ◽  
Experimental Unit ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
In Situ Degradation ◽  
Fermentation Parameters

Abstract Six ruminally cannulated steers (average BW = 791 + 71 kg) were used in a replicated 3 × 3 Latin square experiment to determine the effects of roughage type on rumination, fiber mat characteristics, and rumen fermentation variables. Three roughages were included at 7% (DM basis) in a steam flaked corn-based diet; cotton burrs (CB), wheat silage (WS), or corn stalks (CS). Steers were fitted with a sensory collar to record rumination behaviors in 2-h intervals at the beginning of the experiment. Each 30-d period consisted of a 7-d of recovery, 14-d of diet adaptation, 7-d of rumination data collection (daily and bi-hourly average rumination), 1-d of rumen fluid collection, and 1-d of rumen evacuations. In situ degradation of individual roughages was determined for 4-d after period 3 evacuations. During rumen evacuations, ruminal contents were removed; the rumen fiber mat (RF) was separated from the liquid portion with a 2 mm sieve, weighed, and a subsample was dried. Data were analyzed using the MIXED procedure of SAS with steer as the experimental unit and roughage (CB, WS, and CS) as the main effect. Dry matter intake (DMI) was not different for CB and WS (P = 0.25) and greatest for steers consuming CS diet (P  < 0.01). Roughage type did not influence the weight of the RF dry matter (%; DM; P = 0.92), RF weight (P = 0.69), or RF:DMI ratio (P = 0.29). Daily rumination (min/d) did not differ among roughages (P = 0.40), but min of rumination/kg of DMI was greatest for CS (18.0 min), min/kg of NDF was greatest for WS (89.8 min; P = 0.02), and min/kg of peNDF was greatest for CS (132.4 min; P  < 0.01). Wheat silage had the greatest percentage of soluble DM and CB-R and CS-R (P  < 0.01) had the greatest ruminal degraded DM fraction. Rumen fiber mat did not differ for roughages, although rumination min/kg of DMI and peNDF was greatest for steers consuming CS and WS. In situ degradation determined that CB-R and CS-R had the greatest percentage of ruminal degraded DM. Based on the objective of the experiment, roughage type did not influence daily rumination or fiber mat characteristics.

scholarly journals Processing Characteristics and Sensory Attributes of Bacon Manufactured From Seven Value-Added Cuts of Beef

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
S. Chalupa-Krebzdak ◽  
B. M. Bohrer
Keyword(s):  
Repeated Measures ◽  
Random Effect ◽  
Value Added ◽  
Storage Period ◽  
Sensory Attributes ◽  
Fixed Effect ◽  
Sensory Properties ◽  
Cooking Loss ◽  
Great Opportunity ◽  
Short Plate

ObjectivesThere is great opportunity for the beef industry to add value to cuts that are currently marketed as low value cuts (i.e., cuts from the chuck, round, and flank/plate). The objective was to evaluate the processing characteristics and sensory attributes of bacon manufactured from seven different cuts of beef.Materials and MethodsThe seven cuts evaluated included the brisket (IMPS#120), the clod heart (IMPS#114E; divided horizontally into two halves; referenced as the wide half or silverskin side and the narrow half or non-silverskin side), the flank (IMPS#193), the outside flat (IMPS#171B), and the short plate (IMPS#121A; broken down into the deboned short rib half and the navel half). The cuts were injected using a standard commercial bacon cure (water, salt, corn syrup solids, sodium phosphate, sodium erythorbate, sodium nitrate, sodium bicarbonate, and glycerin; Herman Laue Spice Company Inc.; Uxbridge, Ontario, Canada) to a targeted rested pump uptake of approximately 20% (± 3%). The injected cuts were cooked to an internal temperature of 62°C in a smokehouse (ScottPec, Guelph, Ontario). Following cooking, cuts were cooled to 4°C and then sliced into 4.0 mm slices using a deli slicer. Slices were vacuum packaged, boxed, and stored at 4°C for zero, thirty, sixty, or ninety days. Following the allotted storage period, slices were stored at –20°C until evaluation of sensory attributes and cooking loss. Slices were cooked at 204°C for 15 min in a convection oven. Processing characteristics were conducted in six or seven replications for each cut. Sensory evaluation was conducted on three randomly selected samples for each cut at each of the four storage times (the same samples within each cut was used at each storage time). Processing data were analyzed using PROC GLIMMIX of SAS (v9.4) with fixed effect of cut and random effect of replication. A trained descriptive sensory panel of 6–8 panelists evaluated the differences in oxidative flavor and aroma (using a 4-pt nominal scale), and differences in beef flavor intensity, muscle fiber toughness, and connective tissue amount (using magnitude estimation). Sensory data were analyzed as repeated measures using PROC GLIMMIX of SAS (v9.4) with fixed effect of cut, storage day, and their interaction, and random effects of session, panelist, and replication.ResultsAs expected, dimensions and processing weights differed (P < 0.01) among cuts. Rested pump uptake was not different (P = 0.29) among cuts. Smokehouse yield was greater (P < 0.05) for the brisket, outside flat, and short plate (both halves) compared with the clod heart (both halves) and flank. Bacon slice cooking loss and sensory characteristics are presented in Table 4.ConclusionOverall, this research indicated that a variety of beef cuts can be used to manufacture beef bacon. The differences in sensory properties that were quantified in this study, allow manufacturers to tailor their cut selection to the sensory properties most valued by their consumers. All cuts exhibited oxidative stability when stored up to 90 d.Table 4Bacon slice cooking loss and sensory analysis of bacon manufactured with different beef cuts. Main effects of cut after 0, 30, 60, and 90 d of storage

scholarly journals PSIV-16 Effect of a 1-monoglyceride, 1,2-diglyceride of butyric-, caproic- and caprilic acid, and Avilamycin on performance and intestinal E. coli when fed to piglets challenged with E. coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 188-189
Author(s):  
Michael Coelho ◽  
Peter Ader
Keyword(s):  
Caprylic Acid ◽  
Block Design ◽  
Random Effect ◽  
Fixed Effect ◽  
Experimental Unit ◽  
Infection Model ◽  
E Coli ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
T1 And T2

Abstract This study examined the effect of 1-monoglycerides and 1,2-diglycerides of butyric-, caproic- and caprylic acid (SILOHealth™ 104L [monoglycerides]) and avilamycin on day 14 and 21 post weaning performance of E. coli (GN-Ent and O157:H7) day 5 challenged piglets fed corn/soy diets that met or exceeded current NRC (2012) recommendations. A total of 96, Choice Genetics CG32, piglets were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 piglets/pen x 3 treatments x 8 replications). Pigs were blocked by sex and weight and were orally dosed with 5 ml (1x109 CFU/g, strain GN-Ent and O157:H7) E. coli d 5 after weaning (Ec). The treatment were: NC with Ec; T1=NC + 3 kg/MT monoglycerides; T2=NC+ 80 ppm avilamycin. Least significant difference was used to compare means of treatment. Mortalities and pen removals were weighed and recorded. The E. coli infection model was effective in causing E. coli mortality of 25% at d14 post weaning and 33% at d21 post weaning in NC. T1 and T2 reduced mortality versus NC at d14 (25, 3 and 0%, P < 0.05) and d21 (33, 4 and 0%, P < 0.05), increased ADG at d14 (203, 255 and 259 g, P < 0.05) and d21 (456, 511 and 515 g, P < 0.05), decreased FCR at d21 (1.90, 1.85 and 1.83, P < 0.05), and decreased intestinal E. coli, log 10 of CFU/g, versus NC (4.1, 2.2 and 2.1, P < 0.05). Monoglycerides and avilamycin performance, mortality and intestinal E. coli log 10 were not significantly different at P < 0.05. In conclusion, the use of monoglycerides improved ADG, reduced FCR, reduced mortality and reduced intestinal E. coli log 10 at day 14 and 21 post weaning versus NC and monoglycerides were not significant different (P < 0.05) than avilamycin.

scholarly journals 349 The use of low antioxidant mineral concentrations in the growing diet as a method to increase marbling deposition in feedlot cattle

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 141-142
Author(s):  
Jerad R Jaborek ◽  
Alejandro E Relling
Keyword(s):  
Control Diet ◽  
Random Effect ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Feedlot Cattle ◽  
Experimental Unit ◽  
Beef Steers ◽  
Feedlot Performance ◽  
Dietary Treatments

Abstract The presence of reactive oxygen species has been associated with the differentiation of pre-adipocytes into lipid filled mature adipocytes in vitro. We hypothesized offering a diet without supplemental antioxidant minerals (Cu, Mg, Zn, and Se) to steers during the growing phase, a time when intramuscular fat cells are believed to be proliferating, would promote differentiation of pre-adipocytes, leading to more desirable marbling scores compared with steers supplemented to meet their mineral requirements. After adaptation to the feedlot, 168 Sim-Angus steers were divided into four weight blocks, placed in one of twenty-four pens, and randomly assigned a dietary treatment. Dietary treatments were: 1) no supplemental (Cu, Mg, Zn, and Se) minerals; 2) control diet with supplemented minerals to meet the mineral requirements of growing beef steers (NRC, 2016); 3) Cu, Mg, Zn, and Se concentrations supplemented at twice the concentration of the control diet. After dietary treatments were applied for the 4-wk long growing phase, steers were offered a common finishing diet until reaching a similar backfat thickness until harvest. Feedlot performance and carcass data were analyzed in SAS with pen as the experimental unit in the following model: Yij = μ+Di+wj+eij, where Di was the fixed effect of diet, wj was the random effect of weight block, and eij was the random error. No significant (P > 0.35) treatment effects were found for feedlot performance and carcass measurements. The feedstuffs in the basal diet may have contained sufficient concentrations of antioxidant minerals to meet the mineral requirements of the steers and mask differences in marbling. Additionally, offering a similar diet during the finishing period may have resulted in compensatory marbling growth, which offset marbling differences after the growing phase and resulted in uniform marbling accumulation across dietary treatments.

scholarly journals 126 Energy (ME)-releasing efficacy of graded levels of 6-phytase, Natuphos® E, and a NSPase, Natugrain® TS, in 35 kg pigs fed corn/soy diets

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 70-70
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks
Keyword(s):  
Phytic Acid ◽  
Block Design ◽  
Random Effect ◽  
Fixed Effect ◽  
Experimental Unit ◽  
Fecal Samples ◽  
Randomized Complete Block Design ◽  
Feed Costs

Abstract This study evaluated the metabolizable (ME) release of graded levels of phytase when fed to 35 kg pigs on mash corn/soy diets formulated to meet or exceed current NRC (2012) recommendations. A total of 280 male (PIC 337 x Camborough) pigs were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 pig/pen x 8 treatments x 10 replications). Pigs were blocked by sex and weight. Treatments groups consisted of PC, NC1=PC- 0.17% Ca, - 0.15% aP, -0.02% Na, -200 kcal/kg, T1=NC +1000 FTU/kg phytase+0.15 g/kg NSPase, T2=NC+1500 FTU/kg phytase+0.15 g/kg NSPase, T3=NC+2000 FTU/kg phytase+0.15 g/kg NSPase, T4=NC+2500 FTU/kg phytase+0.15 g/kg NSPase and T5=NC+3000 FTU/kg phytase +0.15 g/kg NSPase. TiO2 was used as the undigestible marker. Feed, rectum grabbed fecal samples were collected at 35 kg pigs. ME was 3407, 3213, 3301, 3329, 3345, 3354 and 3358 Kcal/kg, P < 0.05, respectively. The NC reduced the ME by 194 Kcal/kg versus the PC. 1000, 1500, 2000, 2500 and 3000 FTU/kg released 87, 116, 132, 141 and 145 Kcal/kg, respectively. Least cost formulation reduced net feed costs by $8.63, $9.22, $11.10, $11.90 and $12.39/ton, respectively. In conclusion, Natuphos E by breaking down phytic acid and Natugrain TS by breaking down NSPs released up to 145 Kcal/kg ME in pigs.

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