Influence of a novel consensus bacterial 6-phytase variant on mineral digestibility and bone ash in young growing pigs fed diets with different concentrations of phytate-bound phosphorus

Author(s):  
Charmaine D Espinosa ◽  
Maryane S F Oliveira ◽  
Deepak E Velayudhan ◽  
Yueming Dersjant-Li ◽  
Hans H Stein

Abstract A 20-d experiment was conducted to test the hypothesis that phytase increases nutrient digestibility, bone ash, and growth performance of pigs fed diets containing 0.23%, 0.29%, or 0.35% phytate-bound P. Within each level of phytate, 5 diets were formulated to contain 0, 500, 1,000, 2,000, or 4,000 phytase units (FTU)/kg of a novel phytase (PhyG). Three reference diets were formulated by adding a commercial Buttiauxella phytase (PhyB) at 1,000 FTU/kg to diets containing 0.23, 0.29, or 0.35% phytate-bound P. A randomized complete block design with 144 individually-housed pigs (12.70 ± 4.01 kg), 18 diets, and 8 replicate pigs per diet was used. Pigs were adapted to diets for 15 d followed by 4 days of fecal collection. Femurs were collected on the last day of the experiment. Results indicated that diets containing 0.35% phytate-bound P had reduced (P < 0.01) digestibility of Ca, P, Mg, and K compared with diets containing less phytate-bound P. Due to increased concentration of total P in diets with high phytate, concentrations, apparent total tract digestible P and bone ash were increased by PhyG to a greater extent in diets with 0.29 or 0.35% phytate-bound P than in diets with 0.23% phytate-bound P (interaction, P < 0.05). At 1,000 FTU/kg, PhyG increased P digestibility and bone P more (P < 0.05) than PhyB. The novel phytase increased (P < 0.01) pig growth performance, and pigs fed diets containing 0.35 or 0.29% phytate-bound P performed better (P < 0.01) than pigs fed the 0.23% phytate-bound P diets. In conclusion, the novel phytase (i.e., PhyG) is effective in increasing bone ash, mineral digestibility, and growth performance of pigs regardless of dietary phytate level.

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 45-46
Author(s):  
Charmaine D Espinosa ◽  
Deepak E Velayudhan ◽  
Yueming Dersjant-Li ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that increasing levels of phytase increases mineral digestibility and bone ash by pigs fed diets containing 0.23%, 0.29%, or 0.35% phytate-P. Within each level of phytate, 5 diets were formulated based on corn, soybean meal, and canola meal to contain 0, 500, 1,000, 2,000, or 4,000 phytase units (FTU)/kg of a novel consensus bacterial 6-phytase variant (PhyG). In addition, 3 reference diets were formulated by adding a commercial Buttiauxella phytase (PhyB) at 1,000 FTU/kg to the 3 diets containing no PhyG. A randomized complete block design with 144 pigs (12.70 ± 4.01 kg), 18 diets, and 8 replicate pigs per diet was used. Pigs adapted to diets for 15 d followed by 4 d of fecal collection. Femurs were collected on the last day. Data were analyzed as a 3 × 5 factorial with 3 levels of phytate-P and 5 levels of phytase, and contrast statements were used to compare 1,000 FTU of PhyG with PhyB. Pig was the experimental unit. Diets containing 0.35% phytate-P had reduced (P < 0.01) apparent total tract digestibility (ATTD) of Ca, P, Mg, and K compared with diets containing 0.23% or 0.29% phytate-P, but inclusion of phytase increased (P < 0.01) ATTD of Ca, Na, and K (Table 1). Phytase increased ATTD of P and Mg, but to a greater extent in diets with 0.23 or 0.29% phytate-P than in diets with 0.35% phytate-P (interaction, P < 0.05). Phytase increased bone ash, but to a greater extent if there was 0.35 rather than 0.23 or 0.29% phytate-P in the diets (interaction, P < 0.05). PhyG increased ATTD of P more (P < 0.05) than PhyB. In conclusion, the novel consensus phytase is effective in increasing bone ash and ATTD of Ca, P, Na, Mg and K.


Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan

The aim of this study was to assess the effects of dietary probiotic enhanced liquid acidifier combined with mannan-rich fraction on growth performance, nutrients digestibility in growing pigs. Thirty [(Duroc×Yorkshire)×Landrace] pigs with the average initial BW of 36.75±1.57 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) T1 (basal diet+probiotic 0.1%) and 3) T2 (basal diet+probiotic+mannan rich fraction 0.2%). the data were analyzed as a randomized complete block design using of SAS University Version 4.0. The model included the effects of block (replication) and treatment. Pen served as the experimental unit. During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibility of DM and N tended to increase. To sum up, results in this experiment indicated that dietary [(probiotik×acidifier)×mannan-rich-fraction]  supplementation had a positive effect on growing pigs performance and nutrient digestibility


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 69-70
Author(s):  
Kevin S Jerez Bogota ◽  
Christian Sanchez ◽  
Jimena Ibagon ◽  
Maamer Jlali ◽  
Pierre Cozannet ◽  
...  

Abstract Objective was to determine the effects of supplementing corn-soybean meal-based diets with a multi-enzyme on growth performance and apparent total tract digestibility (ATTD) of nutrients in growing pigs. A total of 276 pigs (BW = 33.99 ± 4.3 kg) were housed by sex in 45 pens of 6 or 7 pigs and fed 5 diets (9 pens/diet) in a randomized complete block design. Diets were positive control (PC); and negative control 1 (NC1) or negative control 2 (NC2) without or with multi-enzyme. The multi-enzyme used supplied at least 1,250, 860, 4,600, and 1,000 units of xylanase, β-glucanase, arabinofuranosidase and phytase per kilogram of diet, respectively. The PC was adequate in all nutrients according to NRC (2012) recommendations and had greater digestible P content than NC1 diet or NC2 diet by 0.134 percentage points. The NC1 diet had lower NE and digestible AA content than PC diet by 3%. The NC2 diet had lower NE and digestible AA content than PC diet by 5%. The diets were fed in 4 phases based on BW; Phase 1: 34–50 kg, Phase 2: 50–75 kg, Phase 3: 75–100 kg, and Phase 4: 100–120 kg. Nutrient digestibility was determined at the end of Phase 1. Overall (34–120 kg BW), pigs fed the PC and NC1 diets did not differ in ADG and ADFI. Pigs fed NC2 diet had lower (PPPP > 0.10). Supplemental multi-enzyme increased (P = 0.047) the ATTD of GE for NC2 diet. In conclusion, the NE and digestible AA and P can be lowered by ≤ 5% in multi-enzyme-supplemented diets without effects on growth performance pigs.


2019 ◽  
Vol 97 (8) ◽  
pp. 3390-3398 ◽  
Author(s):  
Laia Blavi ◽  
Cristhiam J Muñoz ◽  
Jonathan N Broomhead ◽  
Hans H Stein

AbstractTwo experiments were conducted to test the hypothesis that a corn-expressed phytase increases growth performance, bone measurements, and nutrient digestibility by young growing pigs, if added to diets that are deficient in Ca and P. In Exp. 1, 60 pigs (initial BW: 10.78 ± 0.67 kg) were randomly allotted to 6 dietary treatments that included a positive control diet (PC; 0.70% total Ca and 0.60% total P) and a negative control diet (NC; 0.50% total Ca and 0.42% total P). Four additional diets were formulated by supplementing the NC diet with 250, 500, 1,000, or 1,500 phytase units (FTU)/kg. Diets were fed for 28 d and the individual BW of pigs on days 1 and 28 were recorded. Fecal samples were collected from days 25 to 27 to calculate apparent total tract digestibility (ATTD) of Ca and P. On the last day of the experiment, all pigs were euthanized, and the left femur was removed and analyzed for ash, Ca, and P. Results indicated that growth performance, ATTD of Ca and P, and bone ash measurements were reduced (P < 0.05) in NC fed pigs compared with PC fed pigs. However, growth performance, ATTD of Ca and P, and bone ash measurements were improved (linear and quadratic, P < 0.05) by including increasing concentrations of phytase to the NC diet. In Exp. 2, experimental procedures were similar to those used in Exp. 1. Forty-eight pigs (initial BW: 11.15 ± 0.85 kg) were randomly allotted to 6 dietary treatments in a 28-d experiment. Treatments included a PC diet, an NC diet, and 4 diets in which 500 or 1,000 FTU/kg of either the corn-expressed phytase or a commercial microbial phytase were added to the NC diet. Pigs fed the NC diet had reduced (P < 0.01) final BW, ADG, G:F, and bone ash concentrations compared with pigs fed the PC diet. When 500 FTU/kg phytase was fed, no differences were observed in growth performance or bone ash measurements between phytase sources, and there were no differences in growth performance among pigs fed 1,000 FTU/kg of either phytase source or the PC diet. However, regardless of concentration or source of phytase, pigs fed the PC diet had greater (P < 0.001) amount of bone ash, bone Ca, and bone P compared with pigs fed phytase diets. In conclusion, the corn-expressed phytase is effective in improving growth performance, Ca and P digestibility, and bone measurements in pigs fed diets that are deficient in Ca and P.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 375
Author(s):  
Sheena Kim ◽  
Jin Ho Cho ◽  
Younghoon Kim ◽  
Hyeun Bum Kim ◽  
Minho Song

The present study was conducted to evaluate the effects of replacing corn with brown rice on growth performance, nutrient digestibility, carcass characteristics, and gut microbiota of growing and finishing pigs. A total of 100 growing pigs (23.80 ± 2.96 kg BW; 10 weeks of age) were randomly allotted to 4 dietary treatments (5 pigs/pen; 5 replicates/treatment) in a randomized complete block design (block = BW) as follows: corn-soybean meal basal diet (CON) and replacing corn with 50% (GBR50), 75% (GBR75), and 100% (GBR100) of ground brown rice. Each trial phase was for 6 weeks. During the growing period, there were no differences on growth performance and nutrient digestibility among dietary treatments. Similarly, no differences were found on growth performance, nutrient digestibility, and carcass characteristics of pigs during the finishing period among dietary treatments. As a result of the beta diversity analysis, microbial populations were not clustered between CON and GBR100 during the growing phase, but clustered into two distinct groups of CON and GBR100 during the finishing phase. In conclusion, brown rice can be added to the diets of growing-finishing pigs by replacing corn up to 100% without negatively affecting growth performance of the pigs; additionally, this may have an effect on changes in pig intestinal microbiota if continued for a long time.


2020 ◽  
Vol 4 (2) ◽  
pp. 602-615
Author(s):  
Kevin Jerez-Bogota ◽  
Cristian Sánchez ◽  
Jimena Ibagon ◽  
Maamer Jlali ◽  
Pierre Cozannet ◽  
...  

Abstract A study was conducted to determine the effects of supplementing corn–soybean meal-based diets with a multienzyme on growth performance, bone mineralization, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients of growing pigs. A total of 276 pigs (body weight [BW] = 33.99 ± 4.3 kg) were housed by sex in 45 pens of 6 or 7 pigs and fed 5 diets (9 pens/diet) in a randomized complete block design. Diets were positive control (PC); and negative control 1 (NC1) or negative control 2 (NC2) without or with multienzyme. The multienzyme used supplied at least 1,800, 1,244, 6,600, and 1,000 units of xylanase, β-glucanase, arabinofuranosidase, and phytase per kilogram of diet, respectively. The PC diet was adequate in all nutrients according to NRC recommendations and had greater digestible P content than NC1 or NC2 diet by 0.134 percentage points. The PC diet had greater net energy (NE) and standardized ileal digestible amino acids (AA) content than NC1 diet by 3%, and than NC2 diet by 5%. The diets were fed in 4 phases based on BW: Phase 1: 34–50 kg; Phase 2: 50–75 kg; Phase 3: 75–100 kg; and Phase 4: 100–120 kg. Nutrient digestibility and bone mineralization were determined at the end of Phase 1. Overall (34–120 kg BW), pigs fed the PC and NC1 diets did not differ in average daily gain (ADG) and average daily feed intake. Pigs fed NC2 diet had lower (P &lt; 0.05) ADG and gain-to-feed ratio (G:F) than those fed PC diet. Pigs fed PC diet had greater (P &lt; 0.05) bone ash content and ATTD of P than those fed NC1 diet. The ATTD of GE for PC diet was greater (P &lt; 0.05) than that for NC2 diet, and tended to be greater (P &lt; 0.10) than that for NC1 diet. Multienzyme interacted (P &lt; 0.05) with negative control diet type on overall ADG and AID of GE such that multienzyme did not affect overall ADG and AID of GE for the NC1 diet, but increased (P &lt; 0.05) overall ADG and AID of GE for NC2 diet by 5.09 and 8.74%, respectively. Multienzyme did not interact with negative control diet type on overall G:F, bone ash content, AID of AA, and ATTD of nutrients. Multienzyme increased (P &lt; 0.05) overall G:F, AID of methionine, ATTD of GE and P, and tended to increase (P = 0.056) bone ash content. The ADG, bone ash content, and ATTD of GE and P for the multienzyme-supplemented diets were similar to (P &gt; 0.10) PC diet. Thus, NE and digestible AA and P can be lowered by ≤5% in multienzyme-supplemented diets without effects on growth performance and bone ash of pigs.


Author(s):  
S.A. Muhammad ◽  
B.R. Gandi ◽  
Y. Ibrahim

Background: A study was conducted to investigate effects of broiler litter (BL) on growth performance, nutrient utilization, blood haematology and serum biochemistry of West Africa Dwarf (WAD) goats. Methods: Five complete diets were formulated using BL to replace 40% composition of cottonseed cake at 0 (control), 25, 50, 75 and 100%. Thirty WAD bucks weighing 10.57±0.42 kg were randomly allotted to the five treatments in a randomized complete block design (RCBD). Result: The results revealed that daily feed intake (g) and final weight gain (kg) were statistically similar (p greater than 0.05), but daily feed intake cost (N) and feed cost per kg gain (N/kg) decreased (p less than 0.05) across the treatments. Dry matter and organic matter digestibility increased (p less than 0.05), while neutral detergent fibre and acid detergent fibre digestibility decreased (p less than 0.05) across the diets. There was no statistical difference (p greater than 0.05) in N intake, faecal N, urine N and N retained (as % N intake). The serum biochemistry and haematological parameters were statistically similar (p greater than 0.05). Therefore, replacing 100% of CSC with BL maintained body growth; reduced cost of feed; improved nutrient digestibility and maintained normal blood haematology and serum biochemistry.


Fermentation ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 3
Author(s):  
Waewaree Boontiam ◽  
Sumetee Kitipongpysan ◽  
Chalong Wachirapakorn ◽  
Jinsu Hong ◽  
Somsamorn Gawborisut ◽  
...  

This study investigated the growth performance, nutrient digestibility, blood profiles and gut integrity of growing pigs, in response to an increase in pickled freshwater fish residue (PFR) intake following a decrease in salt content. Ninety-six crossbred growing pigs [(Landrace × Large White) × Duroc] with a body weight of 23.65 ± 0.24 kg were randomly assigned to one of four treatments (6 pens/treatment, 4 pigs/pen) in a randomized complete block design. The treatments included: a corn-soybean meal based diet without PFR inclusion (CON) or with PFR addition at 5 (PFR5), 10 (PFR10), and 15% (PFR15), respectively. Desalting via soaking and stirring caused a positive reduction in the salt present on the surface of PFR. Over the 42-day feeding period, an increased level of PFR quadratically increased gain:feed ratio such that gain:feed ratio for PFR10 was greater than that for CON (p < 0.05). Pigs fed PFR10 had greater crude protein digestibility, and lower aspartate aminotransferase and crypt depth than those fed the CON diet (p < 0.05). The linear improvements in crude protein and ether extract digestibility, duodenal villus height, and villus:crypt ratio were observed as the PFR content increased (p < 0.05). However, there were no significant effects on average daily feed intake, dry matter and ash digestibility, blood metabolites (total Ca, P, creatinine, and alkaline phosphatase). Altogether, up to 10% PFR can be included in corn-soybean meal diet without impairing protein digestibility and hepatic enzyme alteration. In fact, administering PFR with lower salt content to growing pigs ultimately promotes their growth performance and gut integrity.


2019 ◽  
Vol 97 (9) ◽  
pp. 3898-3906 ◽  
Author(s):  
Hang Lu ◽  
Aaron J Cowieson ◽  
Jonathan W Wilson ◽  
Kolapo M Ajuwon ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to determine the effects of myo-inositol and phytase on growth performance, plasma metabolites, and nutrient digestibility of growing pigs. In experiment 1, 96 growing pigs with average initial body weight (BW) of 26.2 kg were used in a 25-d growth performance study. Pigs were assigned to four dietary treatments with three pigs per pen and eight replicate pens per treatment in a randomized complete block design. The four treatments were control diet (CD); CD + 2 g/kg inositol; CD + 1,000 FYT/kg phytase and CD + 3,000 FYT/kg phytase. Pigs were weighed individually every week. On day 25, blood sample was collected from one pig per pen to measure plasma metabolites concentrations. In experiment 2, 16 barrows (initial BW 34.8 ± 8.2 kg) were surgically fitted with T-cannulas. Pigs were allotted to four blocks based on BW and assigned to a quadruplicate 4 × 2 incomplete Latin square design with same four dietary treatments and two periods. Ileal digesta samples were collected from each pig on days 6 and 7 of each period to determine apparent ileal digestibility (AID) of nutrients. Phytase supplementation increased final BW and average daily gain (ADG) compared with CD (P < 0.05) with no effects on average daily feed intake (ADFI) and gain to feed (G:F) was higher in 3,000 FYT/kg phytase (P < 0.05). Inositol supplementation had no effects on growth performance. Plasma myo-inositol concentration was increased by inositol supplementation, and 3,000 FYT/kg phytase increased myo-inositol in the plasma by 97.2% (P < 0.05). Plasma P concentration was increased by 1,000 or 3,000 FYT/kg phytase with no effects on alkaline phosphatase (ALP), glucose, triglycerides (TAG), calcium (Ca), and urea concentrations. Phytase supplementation reduced (P < 0.05) the phytate-P concentration in the ileal digesta and increased the digestibility of phytate-P and total P with no effects on the AID of dry matter (DM), gross energy (GE), nitrogen (N), and Ca. In conclusion, the beneficial effects of 3,000 FYT/kg phytase on feed efficiency may due to the increased release of both myo-inositol and phosphorus (P), and may not be solely due to myo-inositol release by this level of phytase.


2020 ◽  
Vol 33 (4) ◽  
pp. 597-604 ◽  
Author(s):  
Yang Jiao ◽  
Xinran Li ◽  
In Ho Kim

Objective: This study was conducted to investigate the effect of zinc aspartic acid chelate (Zn-ASP) on growth performance, nutrient digestibility, blood profiles, fecal microbial and fecal gas emission in growing pigs.Methods: A total of 160 crossbred ([Landrace×Yorkshire]×Duroc) growing pigs with an initial body weight (BW) of 25.56±2.22 kg were used in a 6-wk trial. Pigs were randomly allocated into 1 of 4 treatments according to their sex and BW (8 replicates with 2 gilts and 3 barrows per replication pen). Treatments were as follows: i) CON, basal diet, ii) TRT1, CON+0.1% Zn-ASP, iii) TRT2, CON+0.2% Zn-ASP, and iv) TRT3, CON+0.3% Zn-ASP. Pens were assigned in a randomized complete block design to compensate for known position effects in the experimental facility.Results: In the current study, BW, average daily gain, and gain:feed ratio showed significant improvement as dietary Zn-ASP increased (p<0.05) in growing pigs. Apparent total tract digestibility (ATTD) of dry matter was increased linearly (p<0.05) in pigs fed with Zn-ASP diets. A linear effect (p<0.05) was detected for the Zn concentration in blood with the increasing levels of Zn-ASP supplementation. Lactic acid bacteria and coliform bacteria were affected linearly (p<0.05) in pigs fed with Zn-ASP diets. However, no significant differences were observed in the ATTD of nitrogen, energy and Zn. And dietary Zn-ASP supplementation did not affect fecal ammonia, hydrogen sulfide and total mercaptans emissions in growing pigs.Conclusion: In conclusion, dietary supplementation with Zn-ASP of diet exerted beneficial effects on the growth performance, nutrient digestibility, blood profiles and fecal microbes in growing pigs.


Sign in / Sign up

Export Citation Format

Share Document