β-Hydroxy β-methylbutyrate supplementation to adult Thoroughbred geldings increases type IIA fiber content in the gluteus medius

2021 ◽  
Vol 99 (10) ◽  
Author(s):  
Nicolas I Busse ◽  
Madison L Gonzalez ◽  
Mackenzie L Krason ◽  
Sally E Johnson
Keyword(s):  
Gluteus Medius ◽  
Biceps Femoris ◽  
Biomechanical Properties ◽  
Metacarpophalangeal Joint ◽  
Type I ◽  
Median Frequency ◽  
Hind Limbs ◽  
Integrated Emg ◽  
Improved Performance ◽  
Surface Electromyograms

Abstract Consumption of β-hydroxy β-methylbutyrate (HMB) alters muscle composition and metabolism leading to strength and agility improvements in human athletes. To determine if HMB affects athletic performance and muscle function in horses, Thoroughbred geldings were fed a control (CON; n = 5) or HMB (n = 6) supplement for 6 wk prior to completing a standardized exercise test (SET). Gluteus medius (GM) muscle biopsies were obtained before the SET for fiber typing. Heart rate, biceps femoris (BF) and semitendinosus (ST) surface electromyograms (EMG), and fore and hind limbs metacarpophalangeal joint angles were captured at the gallop of the SET. Results demonstrate that HMB supplementation increased (P < 0.05) the percentage of type IIA and IIA/X muscle fibers in the GM with a corresponding decrease (P < 0.05) in type IIX fibers. The percentage of type I fibers was unaffected by diet. Supplementation with HMB did not result in any measurable effects on performance or biomechanical properties by comparison to CON. Supplementation with HMB resulted in an increase (P < 0.05) in ST median frequency at speeds of 10 m/s and greater. Increasing treadmill speed resulted in an increase (P < 0.05) in stride length and the maximal proximal forelimb fetlock angle, and a decrease (P < 0.05) in stance phase time of the gait cycle. Integrated EMG (iEMG) increased (P < 0.05) with increasing treadmill speeds for both the BF and ST with the BF exhibiting greater (P < 0.05) iEMG values than the ST. In summary, HMB increased the percentage of type IIA GM fibers, which did not translate into improved performance.

scholarly journals Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV)

2021 ◽  
Vol 12 (1) ◽  
pp. 20
Author(s):  
Rabia Nazir ◽  
Arne Bruyneel ◽  
Carolyn Carr ◽  
Jan Czernuszka
Keyword(s):  
Heart Valve ◽  
Crosslinking Density ◽  
Biomechanical Properties ◽  
Collagen Type I ◽  
Type I ◽  
Aortic Heart Valve ◽  
Tissue Engineered Heart Valve ◽  
Human Aortic Valve ◽  
Degradation Properties ◽  
Hybrid Scaffolds

In addition to biocompatibility, an ideal scaffold for the regeneration of valvular tissue should also replicate the natural heart valve extracellular matrix (ECM) in terms of biomechanical properties and structural stability. In our previous paper, we demonstrated the development of collagen type I and hyaluronic acid (HA)-based scaffolds with interlaced microstructure. Such hybrid scaffolds were found to be compatible with cardiosphere-derived cells (CDCs) to potentially regenerate the diseased aortic heart valve. This paper focused on the quantification of the effect of crosslinking density on the mechanical properties under dry and wet conditions as well as degradation resistance. Elastic moduli increased with increasing crosslinking densities, in the dry and wet state, for parent networks, whereas those of interlaced scaffolds were higher than either network alone. Compressive and storage moduli ranged from 35 ± 5 to 95 ± 5 kPa and 16 ± 2 kPa to 113 ± 6 kPa, respectively, in the dry state. Storage moduli, in the dry state, matched and exceeded those of human aortic valve leaflets (HAVL). Similarly, degradation resistance increased with increasing the crosslinking densities for collagen-only and HA-only scaffolds. Interlaced scaffolds showed partial degradation in the presence of either collagenase or hyaluronidase as compared to when exposed to both enzymes together. These results agree with our previous findings that interlaced scaffolds were composed of independent collagen and HA networks without crosslinking between them. Thus, collagen/HA interlaced scaffolds have the potential to fill in the niche for designing an ideal tissue engineered heart valve (TEHV).

High proportion of type I fibres in the gluteus medius muscle of young Norwegian–Swedish coldblooded trotters

2010 ◽  
Vol 7 (02) ◽  
pp. 97-101 ◽  
Author(s):  
Carl Fredrik Ihler ◽  
Tobias Revold ◽  
Stig Larsen ◽  
Birgitta Essén-Gustavsson
Keyword(s):  
Gluteus Medius ◽  
Type I ◽  
Gluteus Medius Muscle

Reciprocal Coactivation Patterns of the Lateral and Medial Quadriceps and Hamstrings during Low- and Moderate-Speed Isokinetic Movement

1998 ◽  
Vol 7 (3) ◽  
pp. 182-196 ◽  
Author(s):  
Ronald V. Croce ◽  
John P. Miller ◽  
Robert Confessore ◽  
James C. Vailas
Keyword(s):  
Knee Joint ◽  
Female Athletes ◽  
Vastus Lateralis ◽  
Biceps Femoris ◽  
Median Frequency ◽  
Vastus Medialis ◽  
Mean Square ◽  
Joint Movement ◽  
Surface Electrodes ◽  
Emg Patterns

The purpose of this study was to examine coactivation patterns of the lateral and medial quadriceps and the lateral and medial hamstrings during low- and moderate-speed isokinetic movements. Twelve female athletes performed isokinetic knee assessments at 60 and 180°/s. Root mean square electromyographic (rmsEMG) activity and the median frequency of the EMG (mfEMG) were determined by placing bipolar surface electrodes on the vastus lateralis (VL), vastus medialis (VM), biceps femoris (BF), and medial hamstrings (MH). Results of rmsEMG indicated that the VM showed almost twice the coactivation of the VL (p< .05), and that the BF showed almost four times the coactivation of the MH (p <.05). Finally, differences were noted in the mfEMG (p< .05), with the VM displaying different recruitment patterns at 180°/s as an agonist compared to that as an antagonist. Results indicated that when acting as antagonists, the VM and BE display the greatest EMG patterns during isokinetic knee joint movement.

scholarly journals A Locomotor Disorder Clinically Similar to Spastic Paresis in an Adult Friesian Bull

1980 ◽  
Vol 17 (3) ◽  
pp. 305-315 ◽  
Author(s):  
R. Bradley ◽  
W.V.S. Wueratne
Keyword(s):  
Skeletal Muscles ◽  
Hind Limb ◽  
Type I ◽  
Type Ii ◽  
Hind Limbs ◽  
Type Ii Cell ◽  
Spastic Paresis ◽  
Flexor Digitorum ◽  
I Cells ◽  
Severe Type

A 5-year-old Friesian stud bull developed a progressive locomotor disorder on return to stud after a period of rest. He had defects in conformation exacerbated by poor condition. The hind limbs were excessively straight. When he stood or moved, the Achilles tendons and their associated muscles were rigid. The disorder clinically resembled spastic paresis of calves. Necropsy showed a degenerative arthropathy in all hind limb joints below the hip. Lesions were also in tendons and skeletal muscles. The M. flexor digitorum superficialis had severe type II cell atrophy with many ring. lobulated and moth-eaten type I cells.

scholarly journals Are skeletally mature female rats a suitable model to study osteoporosis?

2012 ◽  
Vol 56 (4) ◽  
pp. 259-264 ◽  
Author(s):  
Claudia Cardoso Netto ◽  
Vivian Cristine Correia Vieira ◽  
Lizanka Paola Figueiredo Marinheiro ◽  
Sherry Agellon ◽  
Hope Weiler ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Type I Collagen ◽  
Biomechanical Properties ◽  
Mature Female ◽  
Female Rats ◽  
Suitable Model ◽  
Type I ◽  
Age Related ◽  
Female Wistar Rats ◽  
Old Rats

OBJECTIVE: To analyze if female Wistar rats at 56 weeks of age are a suitable model to study osteoporosis. MATERIALS AND METHODS: Female rats with 6 and 36 weeks of age (n = 8 per group) were kept over a 20-week period and fed a diet for mature rodents complete in terms of Ca, phosphorous, and vitamin D. Excised femurs were measured for bone mass using dual-energy x-ray absorptiometry, morphometry, and biomechanical properties. The following serum mar-kers of bone metabolism were analyzed: parathyroid hormone (PTH), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor Κappa B ligand (RANKL), C-terminal peptides of type I collagen (CTX-I), total calcium, and alkaline phosphatase (ALP) activity. RESULTS: Rats at 56 weeks of age showed important bone metabolism differences when compared with the younger group, such as, highest diaphysis energy to failure, lowest levels of OC, CTX-I, and ALP, and elevated PTH, even with adequate dietary Ca. CONCLUSION: Rats at 26-week-old rats may be too young to study age-related bone loss, whereas the 56-week-old rats may be good models to represent the early stages of age-related changes in bone metabolism.

scholarly journals Effects of hormonal growth promotants (HGP) on growth, carcass characteristics, the palatability of different muscles in the beef carcass and their interaction with aging

2008 ◽  
Vol 48 (11) ◽  
pp. 1405 ◽  
Author(s):  
J. M. Thompson ◽  
B. M. McIntyre ◽  
G. D. Tudor ◽  
D. W. Pethick ◽  
R. Polkinghorne ◽  
...  
Keyword(s):  
Carcass Characteristics ◽  
Gluteus Medius ◽  
Biceps Femoris ◽  
Sensory Testing ◽  
Fat Percentage ◽  
Triceps Brachii ◽  
Trenbolone Acetate ◽  
Aging Rates ◽  
Growth Promotants ◽  
Feed Samples

Effects of hormonal growth promotant (HGP) implantation on liveweight, carcass and meat quality measurements were examined using 80 Angus yearling cattle. After entry to the feedlot, 40 steers and 40 heifers were implanted with Revalor-S (28 mg oestradiol and 140 mg trenbolone acetate) and Revalor-H (20 mg oestradiol, 200 mg trenbolone acetate), respectively. Cattle were slaughtered after 55 and 65 days on feed. Samples from the Mm. longissimus dorsi, biceps femoris (the cap and body portions), gluteus medius (the eye and D portions), infraspinatus and triceps brachii were prepared for sensory testing after aging for 5 and 21 days after slaughter. A total of 854 muscle samples were cooked by grill (601) or roast (253) methods and served to consumers using the Meat Standards Australia taste panel protocols. When adjusted to the same initial liveweight, implantation with Revalor-H and Revalor-S resulted in a 4 and 7% increase in slaughter weight, respectively. Implantation resulted in an increased ossification score in steers (P < 0.05), but not in heifers. There was a significant interaction (P < 0.05) between HGP implantation and days aged for shear force. There was a small effect of HGP implants on compression (P < 0.05), but not on cook loss and intramuscular fat percentage. Muscles differed in their response to HGP implantation (P < 0.05) for tenderness, overall liking and palatability scores. Muscles also differed in their aging rates after slaughter (P < 0.05). The greatest response in sensory scores to HGP implantation was found in those muscles that had the highest aging rates. Possible mechanisms by which muscles differed in their response to HGP implantation are discussed.

Relationship Between Speed of Performing Leg Extension With 30 RM Load and the Selected EMG Variables of Selected Quadricep Muscles

2021 ◽  
Vol 10 (1) ◽  
pp. 61-76
Author(s):  
Dhananjoy Shaw ◽  
Deepak Singh ◽  
Umesh Kumar Ahlawat ◽  
Manvinder Kaur ◽  
Dinesh Bhatia
Keyword(s):  
Significant Relationship ◽  
Rank Order ◽  
Vastus Lateralis ◽  
Emg Activity ◽  
Median Frequency ◽  
Healthy Male ◽  
Leg Extension ◽  
Integrated Emg ◽  
Male Subjects ◽  
New Criteria

The aim of the study was to assess the effects of speed of leg extension exercise with 30 RM load on the EMG activity of Vastus Medialis (VM) and Vastus Lateralis (VL). Seventeen healthy male subjects performed 30 repetition of leg extension exercise with 30 RM load in minimum time possible and EMG activity from VM and VL of right lower extremity was recorded simultaneously. Two new criteria, namely gross load speed of movement (GLSM) and relative load speed of movement (RLSM) were developed as the variables of speed of exercise. Selected variables of EMG activity were median frequency (MDF), mean frequency (MNF), root mean square (RMS), and integrated EMG (IEMG). Product moment and rank order correlation were calculated between the selected variables of EMG activity and selected variables of speed of exercise. The selected EMG variables, namely MDF and MNF, reported a negative significant relationship (p<0.05) with selected variables of speed of exercise whereas a selected EMG variables, namely RMS reported a positive significant relationship (p<0.05) with selected with the selected variables of speed of exercise.

scholarly journals Kinematic and EMG Comparison Between Variations of Unilateral Squats Under Different Stabilities

2020 ◽  
Vol 4 (02) ◽  
pp. E59-E66
Author(s):  
Roland van den Tillaar ◽  
Stian Larsen
Keyword(s):  
Muscle Activity ◽  
Rectus Femoris ◽  
Gluteus Medius ◽  
Biceps Femoris ◽  
Peak Force ◽  
Erector Spinae ◽  
Trunk Muscles ◽  
Training Experience ◽  
Free Weight ◽  
Weight Condition

AbstractThe purpose of the study was to compare kinematics and muscle activity between two variations of unilateral squats under different stability conditions. Twelve male volunteers (age: 23±5 years, mass: 80±17 kg, height: 1.81±0.11 m, strength-training experience: 4.3±1.9 years) performed four repetitions with the same external load (≈4RM). Two variations (with the non-stance leg forwards vs. backwards) were performed in a Smith-machine and free-weight condition. The variables were barbell velocity, lifting time and surface electromyography activity of the lower extremity and trunk muscles during the descending and ascending phase. The main findings were 1) peak force was higher when performing the unilateral squats in the Smith machine; 2) peak ascending barbell velocity increased from repetition 3–4 with free weight; and 3) muscle activity from the rectus femoris, vastus lateral, biceps femoris, gluteus medius, and erector spinae increased with repetitions, whereas gluteus, and medial vastus and shank muscles were affected by the conditions. It was concluded that more peak force could be produced because of increased stability. However, peak barbell velocity increased from repetition to repetition in free-weight unilateral squats, which was probably because the participants grew more comfortable. Furthermore, increased instability causes more gluteus and vastus medial activation and foot variations mainly affected the calf muscles.

The Relationship between Integrated EMG and Oxygen Uptake during Treadmill and Bicycle Exercise

1976 ◽  
Vol 20 (23) ◽  
pp. 548-551
Author(s):  
T. Fukunaga ◽  
K. Yuasa ◽  
M. Kobayashi ◽  
T. Miyagawa ◽  
H. Fujimatsu ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Correlation Coefficient ◽  
Rectus Femoris ◽  
Biceps Femoris ◽  
Treadmill Walking ◽  
Emg Activity ◽  
Bicycle Exercise ◽  
Leg Muscles ◽  
Integrated Emg ◽  
Muscle Groups

The aim of this study is to measure the integrated EMG in relation to the oxygen uptake during submaximal treadmill and bicycle exercises. Seven healthy adult subjects performed five minute exercise at three different submaximal work intensities on the same day. The EMG activity in right thigh and leg muscles was measured from m. rectus femoris, m. biceps femoris, m. tibialis anterior and m. gastrocnemius by means of four pairs of surface electrodes sealed with collodion to the skin at a distance of 3 cm apart over the belly of muscles. The EMG activity was not likely modified by the possible fatigue during 5 minutes submaximal exercise in this experiment. In the treadmill walking, there was a rectilinear relationship between integrated EMG activity from four muscle groups and percent of VO2max. On the bicycle exercise the correlation coefficient between them was generally lower than that on the treadmill walking. The product of integrated EMG and volume of the same muscle groups was considerably linearly related to oxygen uptake during treadmill and bicycle exercise (the correlation coefficient was 0.945, p < 0.001 in treadmill and 0.710, p < 0.001 in bicycle).

scholarly journals Observations on Normal Skeletal Muscle of Mature Dogs: A Cytochemical, Histochemical, and Morphometric Study

1982 ◽  
Vol 19 (6) ◽  
pp. 577-595 ◽  
Author(s):  
K. G. Braund ◽  
J. A. Mcguire ◽  
C. E. Lincoln
Keyword(s):  
Skeletal Muscle ◽  
Size Variation ◽  
Biceps Femoris ◽  
Morphometric Study ◽  
Type I ◽  
Triceps Brachii ◽  
Diameter Range ◽  
Age Related ◽  
Mean Diameter ◽  
Fiber Size

Cytochemical, histochemical, and morphometric features of skeletal muscle were evaluated in 140 specimens from five appendicular muscles of 28 dogs which were free of neuromuscular disease. Dogs ranged from six months to 15 years of age. Evidence of degeneration and regeneration, cell reactions and architectural change was found but with an incidence of less than 1% of the total myofiber population in any muscle. The number of fibers with internal nuclei never exceeded 1%. The only changes that appeared to be age-related were fiber size variation associated with the presence of small angular type IIA fibers in muscles of dogs seven years of age and older. Percentages of type I, type IIA, and type IIC fibers remained relatively constant in the various muscles in the age range examined. Type I fibers predominated in medial head of triceps brachii and superficial digital flexor muscles, whereas type IIA fibers were more numerous in biceps femoris and long head of triceps brachii muscles. An approximately equal ratio was found in gastrocranius muscles. In any given muscle, type IIC fibers represented less than 2% of the fibers. Statistically significant differences were found in values for fiber size between groups of dogs weighing more than (mean diameter range = 40 to 50 μm) and less than (mean diameter range = 30 to 40 μm) 15 kilograms.

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