scholarly journals PSVII-29 Validation of prediction equations for digestibility of energy and amino acids in commercial pigs using mini pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 358-359
Author(s):  
Bokyung Hong ◽  
John K Htoo ◽  
Caroline Gonzalez-Vega ◽  
Beob G Kim

Abstract The objective was to validate the prediction equations for digestibility of gross energy and amino acids in commercial pigs using mini pigs. Five commercial crossbred barrows (Landrace × Yorkshire) with initial BW of 62.6 ± 6.1 kg and 5 mini pigs selected from Jeju Island native pigs (M-Pig; Cronex Co., Ltd., Hwaseong, Republic of Korea) with initial BW of 53.4 ± 7.4 kg were used. All pigs were fitted with a T-cannula at the distal ileum, and 5 animals of each breed were randomly allotted to a 5 × 10 Latin square design with 5 diets and 10 periods, respectively. A basal diet contained 98% corn and 4 experimental diets contained 20 or 40% rapeseed meal or wheat distillers dried grains with solubles at the expense of corn. Chromic oxide was included at 0.5% as an indigestible index. The accuracy of previously developed prediction equations for nutrient digestibility of commercial pigs was assessed by regressing measured values from commercial pigs minus predicted values from mini pigs on the predicted values centered to the mean. Comparison of measured and predicted apparent total tract digestibility of gross energy showed that the intercept (0.02 ± 0.81; P = 0.985) indicating mean bias and the slope (0.08 ± 0.15; P = 0.599) indicating linear bias were not different from 0. The equation for standardized ileal digestibility of lysine and arginine did not have mean bias or linear bias (P > 0.05). However, the equations for standardized ileal digestibility of other amino acids had mean bias or linear bias (P < 0.05). In conclusion, energy digestibility of commercial pigs may be predicted by previously developed prediction equations for commercial pigs using mini pigs.

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 85-85
Author(s):  
Charlotte Heyer ◽  
L F Wang ◽  
R T Zijlstra

Abstract Fermentable fiber may increase endogenous losses of P and AA, thereby reducing apparent nutrient digestibility. Acacia gum fiber with medium-to-high fermentability and low viscosity was used to investigate its effect on apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients, and standardized total tract digestibility (STTD) of P in grower pigs. A P-free basal diet (49% corn starch; 18% bovine plasma protein) was formulated to measure basal endogenous P losses (EPL). Three diets were formulated to include 2.5, 5.0, or 7.5% acacia gum fiber at the expense of corn starch in the P-free basal diet. Diets contained 16.1–17.4% CP and 0.31–0.33% total P (DM-basis). The 4 diets were fed to 8 ileal-cannulated barrows (initial BW, 54.6 kg) for four 9-d periods in a double 4 × 4 Latin square. Apparent hindgut fermentation (AHF) was ATTD minus AID. Increasing inclusion of acacia gum quadratically decreased (P < 0.01) AID of DM (∆ 11.1%), linearly decreased (P < 0.05) ATTD of DM (∆ 1.7%) CP (∆ 1.2%), and quadratically increased (P < 0.05) AHF of DM (∆ 9.4%). Basal EPL were 391 and 377 mg/kg DM intake (DMI) for ileum and total tract, respectively. Increasing inclusion of fiber linearly increased (P < 0.05) ileal EPL (∆ 184 mg/kg DMI), and tended to linearly increase (P < 0.10) EPL for total tract (∆ 243 mg/kg DMI). Dietary inclusion of acacia gum tended to linearly decrease (P < 0.10) AID of P, but did not affect (P > 0.10) ATTD, or STTD of P. In conclusion, increasing inclusion of fermentable, low viscous acacia gum fiber decreased diet digestibility of DM and ATTD of CP, but did not affect total tract P digestibility, indicating that increasing fermentable fiber did not increase specific endogenous losses of P in the total tract.


2020 ◽  
Vol 100 (4) ◽  
pp. 729-738
Author(s):  
Peili Li ◽  
Zhiqian Lyu ◽  
Lu Wang ◽  
Bingbing Huang ◽  
Changhua Lai

Two experiments were conducted to evaluate the effects of adding multi-enzyme on the available energy concentration and standardized ileal digestibility (SID) of amino acids of double-low rapeseed expellers (RSE) and rapeseed meal (RSM) fed to growing pigs. In Experiment 1, a total of 36 growing barrows were fed six diets in a replicated 3 × 2 factorial design. Diets included a corn–soybean meal basal diet, RSE diet, and RSM diet with or without multi-enzyme (1000 U g−1 of cellulase, 10 000 U g−1 of xylanase, 2000 U g−1 of glucanase, and 10 000 U g−1 of protease). In Experiment 2, a total of 30 crossbred pigs were randomly allotted to five diets consisting of a nitrogen-free diet and four cornstarch-based diets containing RSE or RSM with or without multi-enzyme. Multi-enzyme supplementation significantly improved (P < 0.05) the metabolizable energy (ME) value and apparent total tract digestibility (ATTD) of ether extract, neutral detergent fiber, and acid detergent fiber, and it tended to increase (P < 0.10) the ATTD of gross energy and digestible energy in ingredients. With the exception of phenylalanine, multi-enzyme supplementation increased (P < 0.05) the SID of crude protein (CP) and all amino acids (AA) in ingredients. Multi-enzyme supplementation enhanced fiber degradation and increased the ME value and SID of CP as well as most AA in double-low rapeseed co-products fed to pigs.


Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1774
Author(s):  
Zhengke Wu ◽  
Jiao Liu ◽  
Jiang Chen ◽  
Shoaib Ahmed Pirzado ◽  
Yang Li ◽  
...  

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.


2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Maryane S F Oliveira ◽  
Markus K Wiltafsky-Martin ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that both the degree of heating and the time that heat is applied will affect the concentration of DE and ME, and the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of amino acids (AA) in 00-rapeseed meal (00-RSM) fed to growing pigs. The nine treatments were prepared using a conventional 00-RSM that was either not autoclaved or autoclaved at 110 °C for 15 or 30 min or at 150 °C for 3, 6, 9, 12, 15, or 18 min. In experiment 1, 20 growing barrows with an average initial BW of 21.2 ± 1.2 kg were randomly allotted to the 10 diets in a replicated 10 × 4 Youden square with 10 diets and four periods in each square. A corn-based basal diet and nine diets containing corn and each source of 00-RSM were formulated. Urine and fecal samples were collected for 5 d after 7 d of adaptation. In experiment 2, nine diets contained one of the nine sources of 00-RSM as the sole source of AA, and an N-free diet that was used to measure basal endogenous losses of AA and CP was formulated. Twenty growing barrows with an initial BW of 69.8 ± 5.7 kg had a T-cannula installed in the distal ileum and were allotted to a 10 × 7 Youden square design with 10 diets and 7 periods. Ileal digesta were collected on days 6 and 7 of each 7-d period. Results from the experiments indicated that there were no effects of autoclaving at 110 °C on DE and ME or on AID and SID of AA in 00-RSM, but DE and ME, and AID and SID of AA were less (P &lt; 0.01) if 00-RSM was autoclaved at 150 °C compared with 110 °C. At 150 °C, there were decreases (quadratic, P &lt; 0.05) in DE and ME, and in AID and SID of AA as heating time increased. In conclusion, autoclaving at 110 °C did not affect ME or SID of AA in 00-RSM, but autoclaving at 150 °C had negative effects on ME and SID of AA and the negative effects increased as heating time increased.


2019 ◽  
Vol 3 (2) ◽  
pp. 662-675 ◽  
Author(s):  
Charmaine D Espinosa ◽  
Su A Lee ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that digestibility of amino acids (AA), gross energy (GE), acid hydrolyzed ether extract (AEE), and neutral detergent fiber (NDF), and values for metabolizable energy (ME) in low-oil distillers dried grains with solubles (DDGS) vary among suppliers. In Exp. 1, the apparent total tract digestibility (ATTD) of GE, AEE, and NDF, and concentration of ME were determined in eight sources of DDGS (sources A, B, C, D, E, G, H, and I). A corn-based basal diet and eight diets containing corn and each source of DDGS were fed to 72 barrows (initial body weight = 18.1 ± 1.3 kg) with eight pigs per diet. Feces and urine were collected for 5 d after 7 d of adaptation. The ME did not differ among the eight sources of DDGS with the exception that DDGS source E contained less (P &lt; 0.05) ME than DDGS source D. The ATTD of GE did also not differ among the eight sources of DDGS, but ME and ATTD of GE in corn were greater (P &lt; 0.05) than in the eight sources of DDGS. However, the ATTD of AEE in corn and the eight sources of DDGS was not different, but the ATTD of AEE in DDGS source E was greater (P &lt; 0.05) than in DDGS source A. The ATTD of NDF in DDGS source D was also greater (P &lt; 0.05) than in DDGS sources E, G, and H, but ATTD of NDF did not differ between corn and the eight sources of DDGS. In Exp. 2, standardized ileal digestibility (SID) of AA was determined in seven sources of DDGS (sources A, B, C, D, E, G, and H). Twenty-four barrows (initial body weight = 63.4 ± 3.4 kg) that had a T-cannula installed in the distal ileum were allotted to a two-period incomplete Latin square design with eight diets. Seven diets were formulated to contain each of the seven sources of DDGS and an N-free diet was also used. Ileal digesta were collected for 2 d after 5 d of adaptation. There were no differences between pigs fed DDGS sources A and B in SID of AA, and the SID of Lys, Met, and Trp did not differ among DDGS sources A, B, and E. However, SID of most indispensable and dispensable AA except Gly were greater (P &lt; 0.05) in DDGS source B than in DDGS sources C, D, E, G, and H. In conclusion, variability in SID of AA, ATTD of NDF and AEE, and ME were observed among the sources of DDGS used in this experiment.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 107-107
Author(s):  
Kevin Jerez-Bogota ◽  
Tofuko A Woyengo

Abstract A study was conducted to determine standardized ileal digestibility (SID) of amino acids (AA) and net energy value (NE) for pigs of heat-pretreated or enzyme-predigested corn whole stillage (WS; slurry material that is dried into DDGS). Ten ileal-cannulated barrows (initial BW = 65.6 ± 3.5 kg) were fed 5 diets in a replicated 5 × 5 Latin square design. The diets were cornstarch-based, containing corn DDGS, untreated WS (C-WS), heat-pretreated WS (Heat-WS) or enzyme-predigested WS (Predigested-WS), and N-free diet. Digestibility of AA in feedstuffs was determined by the direct method. Energy digestibility in feedstuffs was determined by difference from the N-free diet. The WS was heat pretreated at 140 °C and 70 psi for 15 min. Predigestion of the WS was achieved by incubating with multienzyme that supplied xylanase, celullase, α-galactosidase at 2.4, 2.0 and 2.3 mg per gram of WS, respectively, for 12 h at 55 °C. On DM basis, DDGS, C-WS, Heat-WS, Predigested-WS contained 32.8, 30.8, 28.18, and 39.7% CP, 39.8, 51.0, 52.2 and 53.8% NDF, and 4.5, 4.6, 5.7 and 4.5% EE, respectively. The SID of Lys for C-WS (75.5%) was greater (P &lt; 0.05) than that for C-DDGS (67.4%) and Heat-WS (53.9%), but lower (P &lt; 0.05) than for Predigested-WS (84.1%). The NE value for C-WS (2,793 kcal/kg) did not differ from that of C-DDGS (2,668 kcal/kg DM). The NE value for C-WS was greater (P &lt; 0.05) than that for Heat-WS (1,834 kcal/kg DM) and lower than that for Predigested-WS (2,814 kcal/kg DM). In conclusion, enzymatic predigestion of WS increased its SID of Lys and NE value, and hence it can be an attractive technology to increase the nutritive value of corn DDGS for pigs. Heat pretreatment reduced SID of AA and NE values of the WS.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 85-86
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
Mary B Muckey ◽  
Cassandra K Jones ◽  
Hans H Stein

Abstract Two experiments were conducted to determine the effects of extrusion on energy and nutrient digestibility in corn, wheat, and sorghum fed to growing pigs. In Exp. 1, 6 diets based on raw or extruded sources of each grain and an N-free diet were formulated. Seven barrows (14.2 ± 0.9 kg) had a T-cannula installed in the distal ileum and were allotted to a 7 × 7 Latin square. Ileal digesta were collected for 2 d after 5 d of adaptation. Results indicated that extruded grains had greater (P < 0.001) apparent ileal digestibility (AID) of starch than non-extruded grains. Extrusion increased standardized ileal digestibility (SID) of all AA except Pro in corn, but SID of AA in wheat and sorghum was not affected by extrusion (Table 1). In Exp. 2, 48 barrows (15.1 ± 3.7 kg) were allotted to 6 diets based on each of the 6 grains. Pigs were housed individually in metabolism crates and feces and urine were collected quantitatively for 5 d after 5 d of adaptation. The apparent total tract digestibility (ATTD) of GE increased by extrusion of corn or sorghum, but that was not the case for wheat (interaction, P < 0.001). The ATTD of NDF was reduced by extrusion of wheat, but not of corn or sorghum (interaction, P < 0.001). Extrusion reduced (P < 0.05) the ATTD of ADF in all grains. Values for ME were greater in extruded corn and sorghum compared with non-extruded grains, but ME in wheat was not affected by extrusion (interaction, P < 0.001). In conclusion, extrusion of corn, wheat, and sorghum increased AID of starch and SID of all indispensable AA. The ME in corn and sorghum was also increased.


2020 ◽  
Vol 9 (2) ◽  
pp. 41
Author(s):  
Enkhjargal Darambazar ◽  
Daalkhaijav Damiran ◽  
Denise Beaulieu

The objective of this study was to evaluate the effects of dietary inclusion of hydrothermal, phytase, and organic acid pretreated canola meal (CM) on nutrient digestibility in swine. A basal diet barley, wheat, and CM based, was formulated. Four diets containing 30% hydrothermal pretreated CM with 50% moisture and 40&deg;C, phytase (1000 FTU/kg activity), and either citric acid (CA), malic acid (MA), or lactic acid (LA), and a control containing pretreated CM without phytase or organic acid were fed to 12 ileal-cannulated finisher pigs (initial average BW 105.3 &plusmn; 2.7 kg) in a completely randomized design over two periods of 9 days per period. Nutrient composition and phytate content of the diets, ileal digesta, and feces were analyzed and apparent ileal and total tract digestibilities were determined. The inclusion of hydrothermal, phytase, and CA or MA pretreated CM in the diet decreased phytate P (by up to 38.6%) (p &lt; 0.05), consequently increasing available P (by up to 55.6%). Apparent ileal digestibility (AID) was improved for P by 19.9 ‒ 35.1% units and apparent total tract digestibility of DM by 10.3 ‒ 14.8% units, of protein by 6.6 ‒ 12.1% units, and of gross energy by 12 ‒ 17% units across the treatments (p &lt; 0.05), while AID of CP for MA treatment was up by 4.7% units (p &lt; 0.05) relative to the control, indicating improved diet utilization, thus reduced excretion to the environment.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 116-117
Author(s):  
Jinsu Hong ◽  
Jung Wook Lee ◽  
Dan Pettersson ◽  
Tofuko A Woyengo

Abstract Objective of the study was to determine the effect of supplementing a combination of phytase and multi-carbohydrase on standardized ileal digestibility (SID) of amino acid (AA) and net energy (NE) value of carinata meal for growing pigs. Ten ileal-cannulated barrows (initial body weight = 53.9 ± 4.76 kg) were fed 4 diets in a replicated 4 × 4 Latin square design with 2 additional columns to give 10 replicates per diet. Diets included a corn-soybean meal (SBM)-based basal diet, the basal diet with 25% carinata meal, basal diet with 25% carinata meal plus phytase at 2,000 FTU/kg and multi-carbohydrase at 0.2g/kg, and a nitrogen-free diet. The multi-carbohydrase supplied 4 units of xylanase, 10 units of β-glucanase, and 1000 units of pectinase per kilogram of diet. The ratio of corn to soybean meal and soybean oil in carinata meal- containing diets was identical to that in the corn-soybean meal based basal diet to allow calculation of AA and energy digestibility of carinata meal by the difference method. On a DM basis, carinata meal contained 50.2% CP, 0.88% ether extract, 15.37% ADF, 1.82% Lys, 0.96% Met, 1.89% Thr, and 0.64% Trp, respectively. The SID of Lys, Met, Thr, and Trp for carinata meal were 51.4%, 82.2%, 65.9%, and 85.9%, respectively. Supplementation of a combination of phytase and multi-carbohydrase did not affect the SID of AA for the corn-SBM-carinata meal-based diet, and for the carinata meal. However, supplementation of a combination of phytase and multi-carbohydrase improved (P = 0.015) the apparent total tract digestibility of gross energy, and DE and NE values for carinata meal by 9.4, 9.5, and 12.4% respectively. In conclusion, the enzymes used in the current study could be added in carinata meal-based diets for pigs to improve the energy value of the carinata meal.


2014 ◽  
Vol 59 (No. 2) ◽  
pp. 69-83 ◽  
Author(s):  
D.W. Liu ◽  
J.J. Zang ◽  
L. Liu ◽  
N.W. Jaworski ◽  
Z.J. Fan ◽  
...  

Three experiments were conducted to compare raw corn, extruded corn, and dehulled-extruded corn in piglets&rsquo; feeding. In Experiment (Exp.) 1, 8 barrows (19.9 &plusmn; 0.6 kg) were fed experimental diets containing one out of three corn samples and a rice-soybean meal basal diet in a double 4 &times; 4 Latin square design to determine the digestible energy (DE) and metabolizable energy (ME) in the corn samples using the difference method. The DE content in extruded (14.29 MJ/kg) and dehulled-extruded (14.42 MJ/kg) corn was greater (P &lt; 0.05) than in raw corn (13.57 MJ/kg). In Exp. 2, 5 barrows (26.2 &plusmn; 1.3 kg) were fitted with ileal T-cannulas and used in a 5 &times; 5 Latin square design to determine the apparent (AID) and standardized (SID) ileal digestibility of amino acids (AA). The diets comprised the basal diet, the three corn diets from Exp. 1, as well as a nitrogen-free diet to estimate basal endogenous losses of AA. The AID and SID of isoleucine, leucine, lysine, threonine, and valine in dehulled-extruded corn was lower (P &lt; 0.05) than in raw or extruded corn. In Exp. 3, 108 weaned 35&nbsp;days old piglets (8.4 &plusmn; 1.2 kg) were allotted to one of the three diets based on corn type. Weaned pigs fed diets containing extruded or dehulled-extruded corn exhibited reduced (P &lt; 0.05) weight gain and feed intake than pigs fed diets containing raw corn. The diet containing dehulled-extruded corn resulted in a higher incidence of diarrhoea. In summary, extrusion of corn did not result in improvements in digestibility and dehulling corn prior to extrusion appeared to result in heat damage which reduced ileal digestibility of AA. Substitution of raw corn with extruded or dehulled-extruded corn in starter diets formulated to equal quantities of ME and SID AA content did not improve the performance of weaned pigs. &nbsp;


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