Characterization of Cannabidiol in Alternative Biological Specimens and Urine, after Consumption of an Oral Capsule

2020 ◽  
Author(s):  
Alice Ameline ◽  
Jean-Sébastien Raul ◽  
Pascal Kintz
Keyword(s):  
Oral Fluid ◽  
Cannabis Sativa ◽  
Grocery Store ◽  
Exhaled Breath ◽  
The Usa ◽  
Few Data ◽  
Sweat Patch ◽  
Urine Specimens ◽  
Oral Capsule

Abstract Among the hundred cannabinoids present in cannabis sativa indica, cannabidiol (CBD) is a phytocannabinoid discovered in 1940, which can account for up to 40% of the plant’s extract. Medically, it has been proposed to treat convulsions, inflammation, anxiety and nausea. Contrary to the hallucinogenic ingredient of the plant, delta-9-tetrahydrocannabinol, CBD does not seem to have a sedative effect, which can increase its popularity among users. The identification of CBD in blood and urine has been widely described in the scientific literature for several years. Only few data have been reported regarding CBD identification in alternative specimens, such as oral fluid, sweat, exhaled breath and hair. CBD capsules were purchased in the USA from a grocery store and a green capsule containing 22 mg of CBD was orally administered to a 59-year-old healthy man. Oral fluid was collected over 8 h using the NeoSal™ device. Sweat was collected using PharmCheck™ sweat patch technology over 7 days. Exhaled breath was collected with the ExaBreath® DrugTrap device over 8 h. Beard hair was collected 7 and 14 days after administration. Finally, urine specimens were collected over 48 h in plastic tubes without preservative. CBD was only detected in oral fluid at 15 min, at 20 pg/mL. Increasing concentrations, up to 96 pg/patch of CBD, were detected in the sweat patches. CBD was detected for 45 minutes in exhaled breath (Cmax of 302 pg/filter at 30 min). CBD produced a very low but significant chromatographic signal in beard hair, with concentrations <1 pg/mg. Finally, CBD tested positive in urine after enzymatic hydrolysis with a Cmax at 70 ng/mL, after 6 h.

Disability, the ‘good family’ and discrimination in the dismissal of a Presbyterian seminary professor

2018 ◽  
Vol 2 (2) ◽  
pp. 133-161 ◽  
Author(s):  
Valerie Hobbs
Keyword(s):  
Religious Institutions ◽  
Employment Practices ◽  
Conservative Christianity ◽  
Employment Termination ◽  
Good Family ◽  
The Supreme Court ◽  
The Usa ◽  
Witness Statements ◽  
Religious Higher Education

Religious institutions in the USA, under the First Amendment, exhibit great strength in employment termination, given freedom by the Supreme Court to conduct their labour and employment practices with limited scrutiny. This article examines ways in which a Presbyterian seminary board report, justifying its decision not to renew a professor's contract, demonstrates discrimination in its use of the 'good family' ideal prominent within conservative Christianity. Focusing on intertextuality and representation of the professor's wife, a disabled woman, analysis presents evidence of an overall strategy of exclusion. The report consistently demonstrates support for negative witness statements about the professor and his wife while undermining the professor's accounts. The report's characterization of the professor's wife subsumes her identity under her husband's and assumes moral reasons for her disability and chronic illness, consistent with a nouthetic counselling ethos. Findings support the discriminatory potential of the 'good family' ideal, underscoring employees' unique vulnerability within religious higher education institutions. 

Characterization of a new type of mead fermented with Cannabis sativa L. (hemp)

2021 ◽  
Author(s):  
Raffaele Romano ◽  
Alessandra Aiello ◽  
Lucia De Luca ◽  
Rosario Sica ◽  
Emilio Caprio ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
New Type

Optimization and characterization of flavonoids extracted from Cannabis sativa fibers

2021 ◽  
pp. 004051752110277
Author(s):  
Qilu Cui ◽  
Jiawei Li ◽  
Chongwen Yu
Keyword(s):  
Chemical Structure ◽  
Cannabis Sativa ◽  
Ethanol Concentration ◽  
Extraction Process ◽  
Test Results ◽  
Hemp Fiber ◽  
Hemp Fibers ◽  
Structure Surface ◽  
Degumming Process

In this paper, the extraction process of flavonoids from hemp fibers was studied. Response surface methodology (RSM) analysis of the extraction parameters indicated that optimized results would be ethanol concentration 76 vol.%, bath ratio 1:50, and reaction time 139 min; therefore, an optimal extraction rate of flavonoids of 0.2275% can be obtained. The chemical structure, surface morphology and element composition of flavonoid extracts were analyzed. The test results indicated that hemp extract contains flavonoids, which can be used to extract flavonoids from hemp fiber, so as to comprehensively develop hemp fiber and reduce the discharge of waste liquid in the traditional degumming process.

scholarly journals Morphophysiological and molecular characterization of millet (Panicum miliaceum L.) varieties for crop improvement in Western Europe

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Massimo Vischi ◽  
Nicola Zorzin ◽  
Maria Bernhart ◽  
Johanna Winkler ◽  
Dipak Santra ◽  
...  
Keyword(s):  
Western Europe ◽  
Crop Improvement ◽  
Genetic Distances ◽  
Phenotypic Traits ◽  
Panicum Miliaceum ◽  
Novel Foods ◽  
Proso Millet ◽  
Ne Italy ◽  
The Usa

Abstract Background Global warming and issues in favour of a more sustainable agriculture suggest a reconsideration of minor cereals in European agrosystems. Compared to other summer crops, proso millet has a remarkable drought resistance and could be used to improve crop rotation and biodiversity. Proso millet is also increasingly sought by industry to produce novel foods such as those designed for coeliac patients. In this study, a thorough characterization of 11, commercially available, proso millet (Panicum miliaceum L.) varieties was carried out as a preliminary step for crop reintroduction and breeding in Western Europe. Methods The cultivars under evaluation were introduced from Austria, Poland, Russia, and the USA (University of Nebraska–Lincoln). Plants were grown at Udine (NE Italy) and Gleisdorf (Styria, Austria), under greenhouse and field conditions, respectively. Yield components and a range of morphophysiological characters were recorded in both locations. In parallel, 85 SSR markers were tested on DNA samples extracted from randomly chosen plants of each variety and the 12 responsive markers used to genotype the whole variety set. Results Morphometric analyses showed that varieties have several diverging phenotypic traits and architectures. In all instances, yields recorded at field level were much lower than potential yields. In this respect, US selections were comparable to earlier developed European varieties, suggesting that breeding for an increased adaptation is the keystone for a stable reintroduction of millet in Western Europe. Molecular analyses uncovered remarkably low genetic differences and heterozygosity levels within cultivars, confirming millet as an essentially autogamous species; in contrast, large genetic distances were noted among cultivars selected in different environments. Results of SSR genotyping combined with those originating from phenotypic analyses indicated possible crosses to source the genetic variability necessary for selection. Conclusions This study enabled the identification of cultivars that could be used to revitalize the crop in Western Europe and to produce genetically variable hybrid progenies exploitable by breeding.

Experimental Characterization of FSW T-Joints of Light Alloys

2007 ◽  
Vol 344 ◽  
pp. 751-758 ◽  
Author(s):  
Livan Fratini ◽  
Fabrizio Micari ◽  
Antonio Squillace ◽  
G. Giorleo
Keyword(s):  
Rolling Direction ◽  
Mechanical Analysis ◽  
T Joints ◽  
Light Alloys ◽  
Friction Stir ◽  
Relevant Role ◽  
Numerous Data ◽  
Few Data ◽  
Welding Direction

Welding is playing a growing role in transport industry due to relevant advantages it allows. Friction Stir Welding is considered one of the most promising joining technologies, especially when it is applied to light alloys. Focusing attention on FSW of T-joints, several parameters have to be considered, and due to thermo-mechanical features of process, T joints need a dedicated approach. A set of previously developed experiments has shown that the tilt angle plays a relevant role in the joint strength. Furthermore it should be observed that T-joints are very often utilized in aerospace industries since the produced structures are composed of joined skins and stingers. Numerous data are reported in literature about FSW of butt joints, very few data, to authors’ knowledge, exists on T joints. In this paper a micro structural and mechanical analysis has been developed on FSW T-joints of AA 6082 T6 rolled plates, realized setting welding direction both parallel and perpendicular to rolling direction. The obtained results can be considered as a further acquired knowledge in the comprehension and the design of FSW processes.

scholarly journals Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)

2012 ◽  
Vol 93 (10) ◽  
pp. 2171-2182 ◽  
Author(s):  
Ákos Boros ◽  
Csaba Nemes ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
Eric Delwart ◽  
...  
Keyword(s):  
Amino Acid ◽  
Complete Genome ◽  
Bird Species ◽  
Entry Site ◽  
Pathogenic Potential ◽  
Genome Characterization ◽  
Genome Region ◽  
Faecal Samples ◽  
The Usa

Members of the family Picornaviridae are important pathogens of humans and animals, although compared with the thousands of known bird species (>10 000), only a few (n = 11) picornaviruses have been identified from avian sources. This study reports the metagenomic detection and complete genome characterization of a novel turkey picornavirus from faecal samples collected from eight turkey farms in Hungary. Using RT-PCR, both healthy (two of three) and affected (seven of eight) commercial turkeys with enteric and/or stunting syndrome were shown to be shedding viruses in seven (88 %) of the eight farms. The viral genome sequence (turkey/M176/2011/HUN; GenBank accession no. JQ691613) shows a high degree of amino acid sequence identity (96 %) to the partial P3 genome region of a picornavirus reported recently in turkey and chickens from the USA and probably belongs to the same species. In the P1 and P2 regions, turkey/M176/2011/HUN is related most closely to, but distinct from, the kobuviruses and turdivirus 1. Complete genome analysis revealed the presence of characteristic picornaviral amino acid motifs, a potential type II-like 5′ UTR internal ribosome entry site (first identified among avian-origin picornaviruses) and a conserved, 48 nt long ‘barbell-like’ structure found at the 3′ UTR of turkey/M176/2011/HUN and members of the picornavirus genera Avihepatovirus and Kobuvirus. The general presence of turkey picornavirus – a novel picornavirus species – in faecal samples from healthy and affected turkeys in Hungary and in the USA suggests the worldwide occurrence and endemic circulation of this virus in turkey farms. Further studies are needed to investigate the aetiological role and pathogenic potential of this picornavirus in food animals.

Investigating oral fluid and exhaled breath as alternative matrices for anti-doping testing: Analysis of 521 matched samples

2019 ◽  
Vol 176 ◽  
pp. 112810
Author(s):  
Geoffrey D. Miller ◽  
Ryan M. Van Wagoner ◽  
Benjamin J. Bruno ◽  
Jacob D. Husk ◽  
Matthew N. Fedoruk ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Exhaled Breath ◽  
Alternative Matrices ◽  
Matched Samples

scholarly journals Widespread detection and characterization of porcine parainfluenza virus 1 in pigs in the USA

2016 ◽  
Vol 97 (2) ◽  
pp. 281-286 ◽  
Author(s):  
Rachel M. Palinski ◽  
Zhenhai Chen ◽  
Jamie N. Henningson ◽  
Yuekun Lang ◽  
Raymond R. R. Rowland ◽  
...  
Keyword(s):  
Parainfluenza Virus ◽  
The Usa
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