Ultrastructure of Aortic Tissue in Copper-deficient and Control Chick Embryos

1967 ◽  
Vol 91 (3_suppl) ◽  
pp. 283-291 ◽  
Author(s):  
Charles F. Simpson ◽  
J. E. Jones ◽  
R. H. Harms
Keyword(s):  
Aortic Tissue ◽  
Chick Embryos ◽  
Control Chick ◽  
And Control

Tanshinone IIA attenuates elastase-induced AAA in rats via inhibition of MyD88-dependent TLR-4 signaling

VASA ◽  
2014 ◽  
Vol 43 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Tao Shang ◽  
Feng Ran ◽  
Qian Qiao ◽  
Zhao Liu ◽  
Chang-Jian Liu
Keyword(s):  
Nuclear Factor Κb ◽  
Tanshinone Iia ◽  
Myeloid Differentiation ◽  
Aortic Tissue ◽  
Toll Like Receptor ◽  
Sprague Dawley ◽  
Toll Like Receptor 4 ◽  
Tissue Samples ◽  
Myeloid Differentiation Factor ◽  
And Control

Background: The purpose of this study was to determine whether myeloid differentiation factor88-dependent Toll-Like Receptor-4 (TLR-4) signaling contributed to the inhibition of abdominal aortic aneurysm (AAA) by Tanshinone IIA (Tan IIA). Materials and methods: Male Sprague-Dawley rats (n = 12 / group) were randomly distributed into three groups: Tan IIA, control, and sham. The rats from Tan IIA and control groups under-went intra-aortic elastase perfusion to induce AAAs, and those in the sham group were perfused with saline. Only the Tan IIA group received Tan IIA (2 mg / rat / d). Aortic tissue samples were harvested at 24 d after perfusion and evaluated using reverse transcriptase-polymerase chain reaction, Western blot, immunohistochemistry and immunofluorescence. Results: The over-expression of Toll-Like Receptor-4 (TLR-4), Myeloid Differentiation factor 88 (MyD88), Phosphorylated Nuclear Factor κB (pNF-κB) and Phosphorylated IκBα (pIκBα) induced by elastase perfusion were significantly decreased by Tan IIA treatment. Conclusions: Tan IIA attenuates elastase-induced AAA in rats possibly via the inhibition of MyD88-dependent TLR-4 signaling, which may be one potential explanation of why Tan IIA inhibits AAA development through multiple effects.

scholarly journals Loss of potassium from embryonic chick hearts in potassium-free media with and without calcium

1938 ◽  
Vol 124 (837) ◽  
pp. 446-450
Keyword(s):  
Phosphate Buffer ◽  
Direct Evidence ◽  
Watch Glass ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
Intercellular Spaces ◽  
Experimental Solution ◽  
Free Media ◽  
And Control ◽  
Day By Day

Experiments already described (Murray 1938) led to the inference that the cells of the chick embryonic heart lose potassium in potassium-free media. The experiments here described provide direct evidence of this. The hearts were dissected out of 2 ½-3 day chick embryos and placed in the solution PC (Table I) until they had started to beat. They were then thoroughly washed, and were allowed to lie for 5 min. (2 min. in Exp. 1) in the last wash. This last wash is called control A. The solutions used for washing were from the same flasks as the experimental solution. After their passage through control A the hearts were transferred to 2 c.c. of the experimental solution in a Jena watch-glass. After various times in this the hearts were discarded and both the experimental solution and control A were collected. If the experiment extended over more than 1 day the experimental solution and control A were used over again day by day until all the hearts in the experiment had passed through them. The use of control A was necessary for two reasons: ( a ) to show that potassium was not still being washed out of the intercellular spaces at the end of washing ( b ) in experiments lasting over several days the washing solution was fresh each day, but the experimental solution was of course not changed. Hence any small amount of potassium being carried over from the last wash would accumulate in the experimental solution because of the daily increment and might seriously affect the result; but by leaving the hearts for several minutes in the last wash (control A) and by not changing it for fresh on successive days, any such increase would be detected in that solution. In addition to control A, a daily sample (control B) was taken from the same flasks as the solutions used for washing. Details of the solutions are given in Table I ; a phosphate buffer was always used.

scholarly journals EFFECT OF SODIUM MONOFLUOROACETATE ON THE MULTIPLICATION OF INFLUENZA VIRUSES, MUMPS VIRUS, AND PNEUMONIA VIRUS OF MICE (PVM)

1952 ◽  
Vol 96 (6) ◽  
pp. 531-548 ◽  
Author(s):  
William J. Mogabgab ◽  
Frank L. Horsfall
Keyword(s):  
Chick Embryo ◽  
Influenza A ◽  
Virus Titer ◽  
Mumps Virus ◽  
Influenza Viruses ◽  
Mouse Lung ◽  
Influenza B Virus ◽  
Influenza B ◽  
Chick Embryos ◽  
And Control

Sodium fluoroacetate, given after virus inoculation in doses of 3 to 4 mg. per kg. in mice or 2 mg. in chick embryos, caused only a slight delay in the multiplication of the PR8 strain of influenza A virus in the mouse lung and of PR8 or the Lee strain of influenza B virus in the allantoic sac. The quantities of the compound used were sufficient to cause approximately 10 to 20 per cent mortality in mice and 100 per cent in chick embryos. The use of small virus inocula did not markedly increase the effect of sodium fluoroacetate on the multiplication of PR8 or Lee in the chick embryo and maximal titers were obtained in all cases. In contrast to the findings in the chick embryo, sodium fluoroacetate caused a definite delay in the multiplication of Lee virus in the mouse lung but did not affect the final virus titer. Sodium fluoroacetate in like amounts caused only a minimal delay in the multiplication of pneumonia virus of mice (PVM) in the mouse lung or of mumps virus in the chick embryo. With both PVM and mumps virus, maximal titers were obtained almost simultaneously in fluoroacetate and control animals. When three daily injections of the compound were given to mice infected previously with PVM, a definite diminution in the virus titer was demonstrable. However, pretreatment with three daily injections of the compound caused no alteration in the capacity of mice to support the multiplication of PVM. From the results of these experiments, it appears that the cellular metabolic processes blocked by sodium fluoroacetate are not essential for the multiplication of influenza viruses, mumps virus, or pneumonia virus of mice (PVM).

Vitamin B12 in developing chick embryos from vitamin B12-deficient and control eggs

1960 ◽  
Vol 90 (2) ◽  
pp. 250-253 ◽  
Author(s):  
Louise J. Daniel ◽  
Carol A. Hilary ◽  
David W. Yesair
Keyword(s):  
Vitamin B12 ◽  
Chick Embryos ◽  
And Control

Analyse expérimentale de la régulation du nombre des cellules germinales après déficience gonadique chez le poulet

Development ◽  
1974 ◽  
Vol 32 (3) ◽  
pp. 619-635
Author(s):  
Eliane Didier ◽  
Nöel Fargeix ◽  
Yves Bergeaud
Keyword(s):  
Experimental Study ◽  
Germ Cells ◽  
Surgical Excision ◽  
Chick Embryos ◽  
Cellules Germinales ◽  
Control Chick ◽  
The Asymmetry

Experimental study of the regulation of the number of germ cells following gonadial deficiency in the chick Colonization of the genital ridges by germ cells was quantitatively studied in control chick embryos killed at stages 25–29, and in embryos in which a surgical excision of the gonad presumptive area was made previously on the second day. In operated embryos which show a more or less perfect agenesis of one gonad, the number of germ cells counted in genital ridges is lower than the number of germ cells estimated in the same stages of control embryos. The deficit is greater for left gonadic agenesis. The decrease in the total number of germ cells is essentially due to a reduction in the cells colonizing the deficient gonad. There is no excess of germ cells observed in the control gonad. Accordingly, a right side operation strengthens the asymmetry of germ cells distribution, whereas a left side one reduces it. Thus, in birds the regulation of the number of germ cells and the quantitative control of colonisation of the gonads is at the gonad level.

The effect of developing hypertension on the synthesis and accumulation of elastin in the aorta of the rat

1986 ◽  
Vol 64 (1) ◽  
pp. 38-43 ◽  
Author(s):  
F. W. Keeley ◽  
D. J. Johnson
Keyword(s):  
Blood Pressure ◽  
Drinking Water ◽  
Thoracic Aorta ◽  
Male Rats ◽  
Aortic Tissue ◽  
Deoxycorticosterone Acetate ◽  
Young Rats ◽  
Highly Sensitive ◽  
Blood Pressures ◽  
And Control

This report describes an investigation of the effects of developing hypertension on the synthesis and accumulation of insoluble elastin in the thoracic aorta of young rats. Uninephrectomized male rats were made hypertensive by administration of deoxycorticosterone acetate and addition of 1% NaCl to their drinking water. Divergence of systolic blood pressures between treated and control animals and hypertrophy of the vessel began after about 2 weeks of treatment. Coincident with the appearance of hypertrophy, there was an increased accumulation of insoluble elastin in the aorta and a large increase in the capacity of the aortic tissue to synthesize elastin. However, in spite of continued increases in blood pressure and vessel hypertrophy, this effect on elastin synthesis and accumulation was transient. The results of this study suggest that synthesis of elastin in aortic tissue of young rats is highly sensitive to alterations in blood pressure.

Effect of chronic verapamil treatment on ventricular function and growth in chick embryos

1991 ◽  
Vol 261 (1) ◽  
pp. H166-H171 ◽  
Author(s):  
E. B. Clark ◽  
N. Hu ◽  
D. R. Turner ◽  
J. E. Litter ◽  
J. Hansen
Keyword(s):  
Stroke Volume ◽  
Work Load ◽  
Ventricular Pressure ◽  
Saline Control ◽  
Aortic Blood Flow ◽  
Chick Embryos ◽  
Protein Assay ◽  
Null System ◽  
And Function ◽  
And Control

Adjustment of myocardial mass to work load is a fundamental characteristic of the heart. We studied the effect of verapamil, a calcium channel blocker, on growth and function of chick embryonic ventricle. We treated stage 18 chick embryos with verapamil delivered to the extraembryonic vascular bed by a miniosmotic pump and compared them with saline-treated control and untreated embryos. At stages 24, 27, and 29, we measured ventricular pressure and dP/dt by a servo-null system, dorsal aortic stroke volume and dV/dt by pulsed-Doppler, and ventricular and embryo wet weights. Mean myocyte profile area was measured by digital planimetry technique, and cell growth response by DNA and protein assay. Verapamil treatment decreased ventricular pressure in experimental (P less than 0.05) compared with saline control and normal embryos; at stage 27, 1.59 +/- 0.21 vs. 2.17 +/- 0.05 and 2.35 +/- 0.08 (SE) mmHg, respectively. Mean dorsal aortic blood flow decreased in experimental (P less than 0.05) vs. control and normal embryos; at stage 27, 0.98 +/- 0.07 vs. 1.54 +/- 0.10 and 1.56 +/- 0.07 mm3/s, respectively. Stroke volume remained the same in all experimental, normal, and control embryos except at stage 29. Ventricular weight decreased in experimental (P less than 0.05) vs. control and normal embryos; at stage 27, 1.09 +/- 0.07 vs. 1.51 +/- 0.08 and 1.54 +/- 0.11 mg, respectively. Embryo weights, myocyte size, and cytoplasmic fractional volume were similar in all groups. Morphology of ventricles was normal. DNA was lower in experimental (P less than 0.05) compared with control and normal embryos.(ABSTRACT TRUNCATED AT 250 WORDS)

Effect of experimentally induced calcium deficiency on development, metabolism and liver morphogenesis of the chick embryo

Development ◽  
1986 ◽  
Vol 92 (1) ◽  
pp. 207-222
Author(s):  
Tamao Ono ◽  
Rocky S Tuan
Keyword(s):  
Growth And Development ◽  
Serum Proteins ◽  
Serum Levels ◽  
Electrophoretic Analysis ◽  
Calcium Deficiency ◽  
Chick Embryos ◽  
In Ovo ◽  
And Control ◽  
Severe Hypocalcaemia

To study the effects of systemic calcium deficiency on embryonic development, chick embryos maintained in long-term shell-less cultures were compared to control embryos incubated in ovo with respect to various parameters of metabolism and growth. After incubation day 14, retarded growth and development were apparent in shell-less embryos which exhibited severe hypocalcaemia and hyperphosphataemia. A development-specific necrosis of the liver tissues was observed in both shell-less and control embryos, but the frequency and extent of tissue abnormality were significantly greater in the former. Serum levels of lactate dehydrogenase and alkaline phosphatase were considerably elevated in shell-less embryos. Electrophoretic analysis revealed that the relative levels of two major serum proteins were also altered in shell-less embryos.

Effects of Low Frequency Magnetic Fields on Chick Embryos. Dependence on Incubation Temperature and Storage of the Eggs

1986 ◽  
Vol 41 (11-12) ◽  
pp. 1111-1116 ◽  
Author(s):  
Jukka P. Juutilainen
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Incubation Temperature ◽  
Low Frequency ◽  
Chick Embryos ◽  
The Difference ◽  
Incubation Temperatures ◽  
And Control ◽  
And Storage ◽  
The Magnetic Field

Abstract Chick embryos were exposed to sinusoidally oscillating 100 Hz magnetic fields during their first two days of development. The magnetic field strength was 1 A/m. Incubation temperatures of 36.3, 37.0, 38.0 and 38.5 °C were used and the duration of the storage of the eggs before incuba­tion was varied from 1 hour to 4 days. After the incubation, the embryos were examined for abnormalities. When the temperature was 36.3 or 37.0 °C and the eggs were stored for one day or less, the effect of the magnetic field was statistically significant. In these conditions, the percent­ age of abnormal control embryos was low, 8% in 36.3 °C and 5% in 37.0 °C. In the exposed groups the corresponding percentages were 23% (36.3 °C) and 25% (37.0 °C). However, higher temperature and storage of the eggs for 3 to 4 days increased the percentage of abnormal embryos in both the exposed and control groups. The difference between the exposed and control embryos was not significant in these conditions. The results demonstrate the importance of the handling of the eggs in this kind of experiments.

scholarly journals Immunogenicity of experimental trachoma vaccines in baboons: I. Experimental methods, and preliminary tests with vaccines prepared in chick embryos and in HeLa cells

1966 ◽  
Vol 64 (4) ◽  
pp. 513-528 ◽  
Author(s):  
L. H. Collier ◽  
W. A. Blyth
Keyword(s):  
Hela Cells ◽  
Aqueous Suspension ◽  
Research Unit ◽  
Yolk Sac ◽  
Chick Embryos ◽  
Statistical Research ◽  
Degree Of Protection ◽  
Homologous Strain ◽  
Severe Infections ◽  
And Control

Parallel titrations of a strain of trachoma (MRC–221) and one of inclusion conjunctivitis (MRC–4) in the baboon conjunctiva and in chick embryos suggest that ten to twenty 50% egg infective doses are equivalent to one 50% baboon infective dose; but that at least 1000 egg infective doses are needed to induce moderate or severe infections in all of a given number of baboons.For vaccine experiments in baboons, a system of scoring physical signs and presence of inclusion bodies was devised; the significance of differences in vaccinated and control animals in their response to conjunctival challenge was determined by analysis of variance. An aqueous suspension of live MRC–4 grown in the yolk sac was given as two subcutaneous doses and one intravenous dose at weekly intervals, and protected all of six baboons challenged with the homologous strain; three similarly spaced subcutaneous doses were less effective. The immunity induced by this vaccine waned considerably during the ensuing 15 months. Vaccine prepared from a live ‘fast-killing’ variant of MRC–4 grown in HeLa cells was less effective than MRC–4 itself in protecting baboons against infection with the parent strain.Although both yolk sac and HeLa cell vaccines induced the formation of antibody fixing complement with trachoma group antigen, the serum titres in individual animals at the time of challenge were unrelated to the degree of protection; during a 15 month observation period there were pronounced falls in the titres of antibody induced either by vaccination or by challenge with egg-grown TRIC agent.We wish to thank Dr I. Sutherland (M.R.C. Statistical Research Unit) for his helpful advice during the early stages of this work. We are also greatly indebted to Mr P. Avis (Pfizer Ltd.) for his advice and for undertaking the statistical computations; and to Miss Anne Smith and Miss Pay Storey (M.R.C. Trachoma Research Unit) for doing the complement fixation tests.

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