HGG-31. EVALUATE THE ANTI-CANCER OF NOVEL CHELATOR DPC ON GLIOBLASTOMA MULTIFORME CELLS

The translocator protein (TSPO) is an 18-kDa five-transmembrane protein, which is primarily found in the outer mitochondrial membrane. Levels of this protein are up-regulated in the most aggressive and common glioma, glioblastoma multiforme (GM). Levels of TSPO also correlate with GM clinical outcome, suggesting that TSPO may be a novel GM diagnostic imaging agent. Therapeutically, targeting the TSPO may provide a mechanism to abrogate the apoptotic-resistant, invasive and aggressive nature of GM and may also provide a way of targeting other anti-cancer treatments to GM sites. This review highlights recent progress in research on TSPO-based diagnostic imaging and therapeutics for GM.

Download Full-text

Abstract 5415: Targeted nanoparticle based therapy for the treatment of glioblastoma multiforme: Unprecedented anti-cancer activity in highly drug resistant/refractory mouse models of glioblastoma with novel apoptotic nanoparticles which hone to a cell-surface protein f

10.1158/1538-7445.am2014-5415 ◽

2014 ◽

Author(s):

Sarah Hudson ◽

Lilach Agemy ◽

Venkata Ramana Kotamraju ◽

Shweta Sharma ◽

Erkki Ruoslahti ◽

...

Keyword(s):

Glioblastoma Multiforme ◽

Cell Surface ◽

Mouse Models ◽

Surface Protein ◽

Drug Resistant ◽

Cell Surface Protein ◽

Resistant Refractory ◽

Anti Cancer ◽

A Cell ◽

Cancer Activity

Download Full-text

Examining the Role of Specialized DNA Polymerases in the Development of Temozolomide Resistance in Glioblastoma Multiforme

OBM Neurobiology ◽

10.21926/obm.neurobiol.2102096 ◽

2021 ◽

Vol 05 (02) ◽

pp. 1-1

Author(s):

Anthony J. Berdis ◽

Keyword(s):

Glioblastoma Multiforme ◽

Dna Polymerases ◽

Primary Brain Tumor ◽

Dna Lesions ◽

Current Standard ◽

Cellular Mechanisms ◽

Dna Lesion ◽

Anti Cancer ◽

Post Operative Survival

Glioblastoma multiforme (GBM) is an extremely malignant type of primary brain tumor that exhibits a high mortality rate. Current standard therapy involves surgery followed by radiation and treatment with the DNA-alkylating agent, temozolomide (TMZ). While TMZ treatment can extend post-operative survival, most patients develop resistance to TMZ which leads to a significant increase in mortality. At the molecular level, TMZ produces a variety of different DNA lesions including N7-methylguanine, N3-methyladenine, and O6-methylguanine. Although each DNA lesion possesses a unique molecular structure, they all elicit cytostatic and cytotoxic effects against GBM cells. This review article describes the molecular and cellular mechanisms accounting for the anti-cancer activity of TMZ as well as the mechanisms responsible for both inherent and TMZ-induced drug resistance. Special emphasis is placed on understanding the roles that various DNA polymerases play toward the initiation and progression of GBM in addition to mediating resistance to TMZ. This review concludes with discussions on several new approaches that show promise in combating TMZ-resistance, specifically using small molecules to block the replication of DNA lesions catalyzed by various DNA polymerases.

Download Full-text

Baicalein-Enriched Fraction Extracted from Oroxylum indicum (L.) Benth. ex Kurz Leaves Exerts Antioxidant and Inhibitory Effects Against Glioblastoma Multiforme

Processes ◽

10.3390/pr7120963 ◽

2019 ◽

Vol 7 (12) ◽

pp. 963 ◽

Cited By ~ 2

Author(s):

In Nee Kang ◽

Nik Nur Hakimah Nik Salleh ◽

Wan Jie Chung ◽

Chong Yew Lee ◽

Suat Cheng Tan

Keyword(s):

Glioblastoma Multiforme ◽

Crude Extract ◽

Therapeutic Potential ◽

Antioxidant Properties ◽

Extraction Procedure ◽

Inhibitory Effect ◽

Extraction System ◽

Oroxylum Indicum ◽

Fractionation Procedure ◽

Anti Cancer

Glioblastoma multiforme (GBM) is the most malignant subtype of primary brain cancer. To date, standard clinical treatment for GBM is limited in effectiveness and could impose additional side effects. Recently, numerous bioactive compounds isolated from natural plants appear to have beneficial anti-cancer properties. Here, the GBM inhibitory effect of baicalein, a bioactive flavonoid extracted from Oroxylum indicum (L.) Benth. ex Kurz, was evaluated. Firstly, three solvents were used to extract the baicalein. We found that the binary extraction system, using a combination of petroleum ether and methanol (PM), yielded the highest amount of baicalein (15%) compared to the mono extraction system using methanol (13%) or aqueous (0.04%) only. In order to further enhance the baicalein yield in PM crude extract, it was subjected to an enrichment fractionation procedure, which successfully increased the baicalein by nearly two-fold from the initial crude extract (15%) to the enriched fraction 5 (F5) (29%). The enriched F5 not only showed significantly higher (~2.5-fold) antioxidant properties as compared to the crude extract, it was also found to significantly suppress GBM cell proliferation ~2.5-fold better than the crude extract. In conclusion, this study successfully optimized an extraction procedure for increased yield of baicalein metabolite from O. indicum leaves and enhanced its therapeutic potential for GBM treatment.

Download Full-text