The Protective Role of Heme Oxygenase-1 (HO-1) in Chronic Subdural Hematoma

Abstract INTRODUCTION Many studies have revealed that angiogenesis and inflammation are closely linked to the pathophysiology of chronic subdural hematoma (CSDH). The development of CSDH is an inflammatory process that begins as a local inflammatory reaction of the dura meter to injury or external stimuli such as blood or CSF. This process causes neovascularization of the outer membrane of CSDH and vascular hyperpermeability. Heme oxygenase-1 (HO-1) is a key enzyme that catalyzes the degradation of heme and produces biliverdin, ferrous iron, and carbon monoxide. HO-1 expression is induced by oxidative stress, and the increasing expression seems to be protective in animal studies. We hypothesized that HO-1 has a protective impact on the severity of CSDH. METHODS This study is designed to correlate the histopathology of the outer membrane of CSDH and HO-1 level in the CSDH hematoma as an indicator of inflammatory status with the clinical presentation of patient and computed tomography (CT) radiological findings of a consecutive series of patients suffered from CSDH. This is a single-center, perspective cohort study. From 2014 to 2017, we enrolled 97 patients with CSDH, who has undergone surgical intervention (burr-hole drainage or craniotomy evacuation). We collect the clinical data, radiological information, and outer membrane and supernatant of the CSDH. We use enzyme-linked immunosorbent assay (ELISA) to measure the concentration of HO-1 in the supernatant of CSDH. RESULTS A significant negative correlation between the HO-1 and the thickness of CSDH is noted (P = .001). On increasing HO-1 by 1 ng/L, the CSDH thickness decreases by 81 mm (P < .005). CONCLUSION Our study demonstrates that HO-1, an important enzyme in angiogenesis and in the inflammation pathway, has a key impact on CSDH severity. A significant negative correlation between the HO-1 and thickness of CSDH has been proved.

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Circulating Heme Oxygenase-1: Not a Predictor of Preeclampsia but Highly Expressed in Pregnant Women Who Subsequently Develop Severe Preeclampsia

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/6035868 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 2

Author(s):

Valéria C. Sandrim ◽

Mayara Caldeira-Dias ◽

Heloisa Bettiol ◽

Marco Antonio Barbieri ◽

Viviane Cunha Cardoso ◽

...

Keyword(s):

Pregnant Women ◽

Heme Oxygenase ◽

Clinical Symptoms ◽

Severe Preeclampsia ◽

Urine Samples ◽

University Hospital ◽

Enzyme Linked Immunosorbent Assay ◽

Heme Oxygenase 1 ◽

Study Cohort ◽

Potential Biomarker

Preeclampsia is the major cause of maternal and fetal deaths worldwide. Circulating biomarker concentrations to predict preeclampsia must be determined. Therefore, the objective was to evaluate heme oxygenase-1 (HO-1) concentration in both plasma and urine samples from pregnant women before the development of preeclampsia and to identify a potential biomarker for preeclampsia development. We performed a case-control study nested in a prospective study cohort at University Hospital of the Ribeirao Preto Medical School, University of São Paulo (HCFMRP-USP), Ribeirao Preto, Brazil. Of 1400 pregnant women evaluated at 20–25 weeks of gestation, 460 delivered in hospitals outside our institution. Of 940 pregnant women who completed the protocol, 30 developed preeclampsia (cases, 14 cases of severe preeclampsia and 16 cases of mild preeclampsia). Healthy pregnant women (controls, n=90) were randomly selected from the remaining 910 participants. HO-1 concentration was evaluated in plasma/urine samples by using a commercial enzyme-linked immunosorbent assay kit. We found similar HO-1 levels in the plasma and urine for case and control groups. In the subgrouped preeclampsia, lower plasma HO-1 levels were found in mild compared with severe preeclampsia. We conclude that plasma HO-1 levels were not altered at 20–25 weeks of gestation before the manifestation of preeclampsia symptoms. Pregnant women who subsequently develop severe preeclampsia show higher expression of HO-1. This may be indicative of important underlying pathophysiologic mechanisms that differentiate between mild and severe preeclampsia and may possibly be related to a higher prooxidative status even before the development of clinical symptoms.

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Upregulation of Heme Oxygenase-1 by Hemin Alleviates Sepsis-Induced Muscle Wasting in Mice

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/8927104 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Xiongwei Yu ◽

Wenjun Han ◽

Changli Wang ◽

Daming Sui ◽

Jinjun Bian ◽

...

Keyword(s):

Skeletal Muscle ◽

Heme Oxygenase ◽

Muscle Atrophy ◽

Muscle Wasting ◽

Cecal Ligation ◽

Skeletal Muscle Atrophy ◽

Enzyme Linked Immunosorbent Assay ◽

Heme Oxygenase 1 ◽

Protective Effects ◽

Sod Activity

Hemin, an inducer of heme oxygenase-1 (HO-1), can enhance the activation of HO-1. HO-1 exhibits a variety of activities, such as anti-inflammatory, antioxidative, and antiapoptotic functions. The objective of this study was to investigate the effects of hemin on sepsis-induced skeletal muscle wasting and to explore the mechanisms by which hemin exerts its effects. Cecal ligation and perforation (CLP) was performed to create a sepsis mouse model. Mice were randomly divided into four groups: control, CLP, CLP plus group, and CLP-hemin-ZnPP (a HO-1 inhibitor). The weight of the solei from the mice was measured, and histopathology was examined. Cytokines were measured by enzyme-linked immunosorbent assay (ELISA). Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting were used to assess the expression levels of HO-1 and atrogin-1. Furthermore, we investigated the antioxidative effects of HO-1 by detecting malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity. CLP led to dramatic skeletal muscle weakness and atrophy, but pretreatment with hemin protected mice against CLP-mediated muscle atrophy. Hemin also induced high HO-1 expression, which resulted in suppressed proinflammatory cytokine and reactive oxygen species (ROS) production. The expression of MuRF1 and atrogin-1, two ubiquitin ligases of the ubiquitin-proteasome system- (UPS-) mediated proteolysis, was also inhibited by increased HO-1 levels. Hemin-mediated increases in HO-1 expression exert protective effects on sepsis-induced skeletal muscle atrophy at least partly by inhibiting the expression of proinflammatory cytokines, UPS-mediated proteolysis, and ROS activation. Therefore, hemin might be a new treatment target against sepsis-induced skeletal muscle atrophy.

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Serological response of ascarid-free dogs to Toxocara canis infection

Parasitology ◽

10.1017/s0031182000066919 ◽

1981 ◽

Vol 82 (3) ◽

pp. 383-387 ◽

Cited By ~ 13

Author(s):

L. T. Glickman ◽

J. P. Dubey ◽

L. J. Winslow

Keyword(s):

Immunosorbent Assay ◽

Negative Correlation ◽

Significant Negative Correlation ◽

Toxocara Canis ◽

Enzyme Linked Immunosorbent Assay ◽

Serological Response ◽

Eggs And Larvae

SUMMARYAntibodies to Toxocara were measured by enzyme-linked immunosorbent assay (ELISA) in sera of ascarid-free dogs which had been fed 0,100 or 10000 embryonated T. canis eggs. Dogs fed 10000 eggs developed higher titres than those given 100 eggs and larvae failed to complete their migration to the gut. In addition, in dogs given 100 eggs, there was a significant negative correlation between the number of worms in the gut and granulomatous foci in the lung. The data suggest that the ELISA is sensitive and specific for Toxocara infection in dogs.

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Chronic Subdural Hematomas: To Drain or Not to Drain?

Neurosurgery ◽

10.1227/00006123-198502000-00010 ◽

1985 ◽

Vol 16 (2) ◽

pp. 185-188 ◽

Cited By ~ 76

Author(s):

Thomas Marc Markwalder ◽

Rolf W. Seiler

Keyword(s):

Clinical Improvement ◽

Closed System ◽

Subdural Hematoma ◽

Drainage System ◽

Chronic Subdural Hematoma ◽

Burr Hole ◽

Postoperative Phase ◽

Consecutive Series ◽

Neurological Improvement ◽

Burr Hole Evacuation

Abstract A consecutive series of 21 adult patients with chronic subdural hematoma was studied in respect to postoperative resolution of subdural collections and clinical improvement after burr hole evacuation without subdural drainage. This series was compared to a previously studied series of patients with chronic subdural hematoma in whom postoperative closed system drainage had been installed. Using the identical protocol for treatment and postoperative follow-up, we obtained identical results with respect to time-related neurological improvement and persistence of subdural collections in the undrained and drained series, except that the steadily progressive clinical improvement during the early postoperative phase (24 hours) in all cases of the drained series was not universal in the undrained cases. Our study suggests that, to avoid the possibility of early postoperative clinical deterioration, burr hole craniostomy and closed system drainage is advisable. We think that subdural drainage is not necessary when the installation of the drainage system seems to be technically difficult, as it may be in cases with considerable perioperative cortical expansion.

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INCREASED URINARY 8-OXO-7,8-DIHYDRO-2′-DEOXYGUANOSINE EXCRETION IN A SAMPLE OF EGYPTIAN CHILDREN WITH BETA THALASSEMIA MAJOR: MARKER FOR LIPID PEROXIDATION-INDUCED DNA DAMAGE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21045 ◽

2017 ◽

Vol 10 (12) ◽

pp. 171

Author(s):

Mona A Elabd ◽

Dina Abu Zeid ◽

Marwa A Elhady ◽

Maged A El Wakeel ◽

Ghada M El-kassas ◽

...

Keyword(s):

Lipid Peroxidation ◽

Dna Damage ◽

Urinary Excretion ◽

Serum Ferritin ◽

Negative Correlation ◽

Significant Negative Correlation ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Increased Risk ◽

Egyptian Children

Objective: The objective of this research was to evaluate oxidative stress status in children with β-thalassemia major.Methods: Our study was conducted in children with β-thalassemia aged from 5 to 15 years. Investigate the urinary excretion of human 8-oxo-7,8- dihydro-2′-deoxyguanosine, which will be analyzed by enzyme-linked immunosorbent assay. To investigate serum levels of antioxidant enzymes include glutathione s-transferase (GST) and catalase (CAT).Results: We found a significant elevation of the urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine level with p=0.001 compared to control group, a significant reduction of both GST and CAT p=0.05 and 0.03, respectively, compared to control group. There was a significant negative correlation between urinary 8-oxo-7,8-dihydro-2′-deoxyguanisine and CAT level, r=−0.378, p=0.016, hemoglobin - r=−0.610, p=0.001, hematocrit (%) - r=−0.478, p=0.002, while a significant positive correlation between urinary 8-oxo-7,8-dihydro-2′-deoxyguanisine and alanine aminotransferase - r=0.547, p=0.001, and serum ferritin - r=0.391, p=0.013. There was a significant negative correlation between CAT and serum ferritin - r=−0.320, p=0.44.Conclusion: We conclude that the strongly increased urinary excretion 8-oxo-7,8=dihydro-2′-deoxyguanisine indicates elevated lipid peroxidation induced DNA damage in internal organs such as the liver. These highly pro mutagenic lesions may contribute to the increased risk of thalassemia patients to develop hepatocellular carcinoma.

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Gradation density hematoma is a predictor of chronic subdural hematoma recurrence associated with inflammation of the outer membrane

Clinical Neurology and Neurosurgery ◽

10.1016/j.clineuro.2020.105839 ◽

2020 ◽

Vol 194 ◽

pp. 105839

Author(s):

Yu Shimizu ◽

Cheho Park ◽

Kazuhiko Tokuda

Keyword(s):

Outer Membrane ◽

Subdural Hematoma ◽

Chronic Subdural Hematoma

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Subdural Hyperintense Band on Diffusion-Weighted Imaging of Chronic Subdural Hematoma Indicates Bleeding From the Outer Membrane

Neurologia medico-chirurgica ◽

10.2176/nmc.45.125 ◽

2005 ◽

Vol 45 (3) ◽

pp. 125-131 ◽

Cited By ~ 19

Author(s):

Seikou KUWAHARA ◽

Masaaki FUKUOKA ◽

Yoko KOAN ◽

Hirohisa MIYAKE ◽

Yuko ONO ◽

...

Keyword(s):

Outer Membrane ◽

Subdural Hematoma ◽

Diffusion Weighted Imaging ◽

Chronic Subdural Hematoma ◽

Diffusion Weighted

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Correlation between Serum Osteopontin and miR-181a Levels in Allergic Rhinitis Children

Mediators of Inflammation ◽

10.1155/2016/9471215 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Wenlong Liu ◽

Qingxiang Zeng ◽

Renzhong Luo

Keyword(s):

Allergic Rhinitis ◽

Negative Correlation ◽

Quantitative Polymerase Chain Reaction ◽

Allergic Airway Inflammation ◽

Serum Levels ◽

Significant Negative Correlation ◽

Enzyme Linked Immunosorbent Assay ◽

Th2 Cytokine ◽

Th2 Immune Response ◽

Th1 Cytokine

Background. Osteopontin (OPN) has been proved to be associated with allergic airway inflammation. However, the roles of OPN and its regulation in childhood allergic rhinitis (AR) are poorly understood.Objective. This study aims to evaluate the expression of OPN and miR-181a in children with AR and their association with Th1/Th2 immune response.Methods. Children who suffered from AR were included along with control subjects. Serum was collected to examine the level of OPN and Th1/Th2 cytokines by enzyme-linked immunosorbent assay (ELISA) and the level of miR-181a by quantitative polymerase chain reaction (qPCR).Results. Children with AR had significantly higher serum levels of OPN and lower serum levels of miR-181a than healthy controls. Furthermore, serum levels of OPN were positively correlated with Th2 cytokine and negatively correlated with Th1 cytokine. On the contrary, miR-181a level had a negative correlation with IL-4/IL-5 and positive correlation with IFN-γ/IL-12. More importantly, there was also significant negative correlation between OPN and miR-181a.Conclusion. The OPN protein and miR-181a levels may serve as predictors of disease severity in childhood AR and appear to be promising targets for modulating AR.

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Role of outer membrane histopathology and comparison of clinico-radiological aspects of chronic subdural hematoma in different age groups: a retrospective study

International Surgery Journal ◽

10.18203/2349-2902.isj20172539 ◽

2017 ◽

Vol 4 (7) ◽

pp. 2164

Author(s):

Nilesh Vishnu Potdar ◽

Suresh Kumar S. ◽

Bhavadasan Kaplinghat

Keyword(s):

Outer Membrane ◽

Old Age ◽

Subdural Hematoma ◽

Chronic Subdural Hematoma ◽

Young Age ◽

Histopathological Study ◽

Age Group ◽

Group Patient ◽

Membrane Type

Background: The incidence of chronic subdural hematoma (CSDH) has been found to increasing in younger patients. This study was aimed to evaluate the role of outer membrane histopathology and comparison with the clinic-radiological aspects of chronic subdural hematoma in different age groups.Methods: Cases of CSDH admitted to the Neurosurgery department during January 2014 and December 2016 were included in the study. They were analyzed clinically, radiologically like site, size, thickness in computed tomography, the attenuation value and midline shift. Histopathological features were also recorded. Cases of acute and chronic sub dural hematoma which were managed conservatively irrespective of age and sex were excluded from the study.Results: Total 196 patients were included with median age of 66 yrs. The most common histopathological type of membrane was the scar inflammatory membrane (Type IV) in 43% of cases followed by hemorrhagic inflammatory membrane (Type III) in 31% of cases while the scar inflammatory type of membrane (Type II) was in 26% of cases. Young age group patient having less thickness of hematoma (<2cm) and having hyper density on CT scan. Old age group had more thickness(3.2cm) and mixed density with multiple layering.Conclusions: Young age group patient having less thickness of hematoma and hyper density compared to old age group. Recurrence and bilateral disease were more common in old age group associated with brain atrophy. Histopathological study completes the spectrum of CSDH in terms of severity of disease and overall prognosis of patient.

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