Comparison of Gonadal Hormone Levels in Turkey Embryos Incubated in Long-Term Shell-Less Culture and In Ovo

ObjectiveThis study investigated the influence of ageing – in particular the decrease of gonadal hormone levels during the ageing process – on the memory and the levels of DNA damage in the hippocampus of female rats.MethodsThree groups of female Wistar rats were investigated: Group I consisted of non-ovariectomised, adult animals (6 months old); Group II consisted of non-ovariectomised, aged animals (18 months old); and Group III consisted of ovariectomised, aged animals (18 months old). The memory of the animals in these groups was examined via novel object recognition and inhibitory avoidance tests. The hippocampus tissue samples of all animals were obtained via biopsy and used to quantify the DNA damage using a Comet Assay.ResultsAccording to our findings, the process of ageing results in a change during the behavioural tests. To prevent genotoxic damage to the hippocampus caused by the ageing process, lowered hormone levels seem to be part of a protective biochemical mechanism in the body of rats. Animals that were previously submitted to an ovariectomy adapted better to these lower levels of hormones.ConclusionOur results indicate that ovariectomy can provide beneficial long-term effects on the memory. However, this could be specific to the kind of memory examined, as the aversive memory deficits caused by ageing were not affected by ovariectomy.

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Gender Differences in Depression and Sex Hormones among Patients Receiving Long-Term Opioid Treatment for Chronic Noncancer Pain in Taiwan—A Multicenter Cross-Sectional Study

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18157837 ◽

2021 ◽

Vol 18 (15) ◽

pp. 7837

Author(s):

Shung-Tai Ho ◽

Tso-Chou Lin ◽

Chun-Chang Yeh ◽

Kuang-I Cheng ◽

Wei-Zen Sun ◽

...

Keyword(s):

Sexual Function ◽

Sex Hormones ◽

Sex Hormone ◽

Cross Sectional ◽

Chronic Noncancer Pain ◽

Depression Diagnosis ◽

Opioid Treatment ◽

Hormone Levels ◽

Noncancer Pain

Background: Long-term use of opioids for chronic noncancer pain is associated with sex hormone disturbances. The interferences with sex hormones, sexual function, and depression were investigated in patients with chronic noncancer pain. Methods: A cross-sectional multicenter survey was conducted on 170 officially registered outpatients receiving long-term opioid treatment in nine medical centers in Taiwan between October 2018 and July 2019. Serum sex hormone levels were examined after the collection of self-administered questionnaires containing the Taiwanese version of the Brief Pain Inventory, depressive status, and sexual function interference. Results: Among 117 (68.8%) questionnaire responses from 170 enrolled outpatients, 38 women and 62 men completed the sex hormone tests, among whom only 23 (23%) had previously received blood hormone tests. Low serum total testosterone levels were detected in 34 (89.5%) women (<30 ng/dL) and 31 (50%) men (<300 ng/dL). Over 60% of women and men reported reduced sexual desire and function despite a nearly 50% reduction in pain intensity and daily function interference over the previous week after opioid treatment. Women generally had higher risks of a depression diagnosis (p = 0.034) and severe depressive symptoms (p = 0.003) and nonsignificantly lower opioid treatment duration (median 81 vs. 120 months) and morphine milligram equivalent (median 134 vs. 165 mg/day) compared with men. Conclusions: This survey demonstrated the high prevalence of depression diagnosis, low sex hormone levels, and reduced sexual function among Taiwanese patients with chronic noncancer pain receiving prolonged opioid therapy. Regular hypogonadal screenings are recommended for further management.

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LRF and TRF test during long-term danazol treatment: increase of the LH and FSH responses but decrease of the prolactin and TSH responses

Acta Endocrinologica ◽

10.1530/acta.0.1040001 ◽

1983 ◽

Vol 104 (1) ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

J. Leppäluoto ◽

L. Rönnberg ◽

P. Ylöstalo

Keyword(s):

Clinical Symptoms ◽

Follicular Phase ◽

Blood Samples ◽

Hormone Levels ◽

Coefficients Of Variation ◽

The Mean ◽

Thyroid Hormone Levels ◽

Low Serum

Abstract. Seven patients suffering from severe endometriosis were treated with danazol 200 mg × 3 daily for 6 months. Clinical symptoms were alleviated and menses disappeared in response to the treatment. After cessation of the treatment the menstrual bleedings returned in 1–3 months. Blood samples for determination of gonadotrophins, prolactin (Prl), oestradiol (E2), progesterone, thyroid hormones and thyrotrophin in radioimmunoassays were taken and a combined TRF and LRF test carried out in the follicular phase before treatment, at the 6th month of treatment and after reappearance of the first menses. There were no statistically significant changes in the basal levels of serum FSH, LH or TSH during the danazol treatment. Neither was there any change in episodic secretions of FSH, LH or Prl, as determined by the mean coefficients of variation of the hormone levels in seven consecutive samples taken at 20 min intervals. On the other hand, serum E2, Prl and thyroid hormone levels were significantly decreased in the 6th month of treatment. In the TRF-LRF test the responses of serum FSH and LH were significantly higher and those of serum Prl and TSH significantly lower during danazol treatment than before. Prl responses remained lowered after the treatment. It appears that low serum oestrogen levels, induced by the danazol treatment, sensitize the pituitary gonadotrophs to exogenous LRF, but make the sensitivity of thyrotrophs and lactotrophs lower to exogenous TRF. These results thus indicate that danazol does not make the pituitary gonadotrophs insensitive to LRF, but danazol may rather inhibit the secretion of hypothalamic LRF.

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The Effect of Long-Term Metabolic Control on Free Thyroid Hormone Levels in Diabetics during Insulin Treatment

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328702400507 ◽

1987 ◽

Vol 24 (5) ◽

pp. 466-469 ◽

Cited By ~ 2

Author(s):

J H Parr

Keyword(s):

Thyroid Hormone ◽

Metabolic Control ◽

Insulin Treatment ◽

Density Lipoprotein ◽

Hdl Cholesterol ◽

Hormone Levels ◽

Free Thyroid Hormone ◽

The Mean ◽

Thyroid Hormone Levels

Serum concentration of free T3 and, in female patients, FT4, were found to be lower in 20 asymptomatic, moderately-poor or well controlled, diabetics treated with insulin than in a group of non-diabetic subjects. Over a mean 3-month period of the study a significant fall occurred in HbA1 concentration in both groups of diabetics without change in free thyroid hormone levels. The mean capillary blood glucose, fasting free insulin and fasting lipid concentrations, other than high density lipoprotein (HDL) cholesterol, did not change. No correlations were found between the changes in HbA1 and free thyroid hormone concentrations. Improvement in long term metabolic control did not influence free thyroid hormone levels in well controlled and moderately-poor controlled diabetics, taking insulin.

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Long-term valproate treatment induces changes in ovarian morphology and serum sex steroid hormone levels in female Wistar rats

Seizure ◽

10.1053/seiz.1999.0342 ◽

1999 ◽

Vol 8 (8) ◽

pp. 490-493 ◽

Cited By ~ 35

Author(s):

Erik Taubøll ◽

Jouko I.T. Isojärvi ◽

Hanne Flinstad Harbo ◽

Arto J. Pakarinen ◽

Leif Gjerstad

Keyword(s):

Wistar Rats ◽

Steroid Hormone ◽

Sex Steroid ◽

Sex Steroid Hormone ◽

Hormone Levels ◽

Ovarian Morphology ◽

Female Wistar Rats

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The validation of estradiol extraction and analysis from hair

10.31234/osf.io/knuxs ◽

2020 ◽

Author(s):

Wen Wang ◽

Lotte van Dammen ◽

Shannin N. Moody ◽

Jeff Kiesner ◽

Jenae M. Neiderhiser ◽

...

Keyword(s):

Adult Women ◽

Hormone Levels ◽

The Past ◽

Extraction Protocol ◽

Hair Samples ◽

Double Extraction ◽

Emerging Adult Women ◽

Estradiol Levels ◽

Hormonal Levels

Background. Saliva is a popular biospecimen for the measurement of hormones, yet fluctuations in hormone levels limit the extent to which saliva can address focusing on basal or long-term levels. Hair steroid assays return basal hormonal levels by collapsing across short-term hormonal variability, including menstrual cyclicity. Here we sought to validate a hair bioassay methodology that can capture stable estradiol levels from both human and monkey hair samples. Methods. Three projects were involved to examine hair-saliva correspondence and estradiol stability in hair. Project 1. Saliva samples were collected once per week for two cycles in 11 emerging adult women. Hair samples were collected at the end of each menstrual cycle and were segmented by 1 cm for the first 4 cm to reflect the past four serial months’ hormone levels. Project 2. Hair samples collected from 23 adolescent participants (Mage = 14.1, 56.5% female) were cut to three 1.5 cm segments from the scalp end. Project 3. Two hair samples were collected from two adjacent skin areas on each monkey (N = 8, 75% males). Whole hair samples were sheared and used for assay without segmentation. Hair biospecimens were processed using a double-extraction protocol validated in this study, then assayed using commercially-available enzyme-immuno-assays for estradiol. Results. Project 1. Hair estradiol concentrations were significantly associated with averaged saliva estradiol levels (r = 0.77, p< .05). Estradiol levels in two consecutive segments were significantly associated (1st vs. 2nd: r = .63, p < .01; 2nd vs. 3rd: r = .49, p < .05; 3rd vs. 4th: r = .53, p < .05). Project 2. Estradiol concentrations were significantly correlated between the first two successive hair segments from the scalp end (r = .69, p < .01). Project 3. Estradiol levels in the two hair samples from each monkey were significantly correlated (r = .66, p < .05).Discussion. Results suggest hair captures valid and reliable average estradiol concentrations using a double-extraction protocol that is applicable for both human and monkey hair. The measurement of hair provides valuable information on individual differences in average estradiol levels across months. Results also indicate that it is feasible and reliable to collect the first 2-3 centimeters of studies in which basal estradiol levels in the past 2 to 3 months are of interest as a stable hormonal index for different species.

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In Ovo Effects of Two Organophosphate Flame Retardants—TCPP and TDCPP—on Pipping Success, Development, mRNA Expression, and Thyroid Hormone Levels in Chicken Embryos

Toxicological Sciences ◽

10.1093/toxsci/kft100 ◽

2013 ◽

Vol 134 (1) ◽

pp. 92-102 ◽

Cited By ~ 126

Author(s):

Amani Farhat ◽

Doug Crump ◽

Suzanne Chiu ◽

Kim L. Williams ◽

Robert J. Letcher ◽

...

Keyword(s):

Thyroid Hormone ◽

Mrna Expression ◽

Flame Retardants ◽

In Ovo ◽

Chicken Embryos ◽

Hormone Levels ◽

Thyroid Hormone Levels

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Secretome of Senescent Adipose-Derived Mesenchymal Stem Cells Negatively Regulates Angiogenesis

International Journal of Molecular Sciences ◽

10.3390/ijms21051802 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1802 ◽

Cited By ~ 5

Author(s):

Andrey Ratushnyy ◽

Mariia Ezdakova ◽

Ludmila Buravkova

Keyword(s):

Replicative Senescence ◽

Umbilical Vein ◽

Chorioallantoic Membrane ◽

Dot Blot ◽

In Ovo ◽

Angiogenic Potential ◽

Associated Proteins ◽

Migration Capacity ◽

Umbilical Vein Endothelial Cells

Nowadays, paracrine regulation is considered as a major tool of mesenchymal stem cell (MSC) involvement in tissue repair and renewal in adults. Aging results in alteration of tissue homeostasis including neovascularization. In this study, we examined the influence of replicative senescence on the angiogenic potential of adipose-derived MSCs (ASCs). Angiogenic activity of conditioned medium (CM) from senescent and “young” ASCs was evaluated in chorioallantoic membrane (CAM) assay in ovo using Japanese quail embryos. Also, the formation of capillary-like tubes by human umbilical vein endothelial cells (HUVECs) in 3D basement membrane matrix “Matrigel” and HUVEC migration capacity were analyzed. Multiplex, dot-blot and gene expression analysis were performed to characterize transcription and production of about 100 angiogenesis-associated proteins. The results point to decreased angiogenic potential of senescent ASC secretome in ovo. A number of angiogenesis-associated proteins demonstrated elevation in CM after long-term cultivation. Meanwhile, VEGF (key positive regulator of angiogenesis) did not change transcription level and concentration in CM. Increasing both pro- (FGF-2, uPA, IL-6, IL-8 etc.) and antiangiogenic (IL-4, IP-10, PF4, Activin A, DPPIV etc.) factors was observed. Some proangiogenic genes were downregulated (IGF1, MMP1, TGFB3, PDGFRB, PGF). Senescence-associated secretory phenotype (SASP) modifications after long-term cultivation lead to attenuation of angiogenic potential of ASC.

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