scholarly journals P096 Analysis of lung tissue samples from the FinnishIPF cohort study

QJM ◽  
2016 ◽  
Keyword(s):  
Cohort Study ◽  
Lung Tissue ◽  
Tissue Samples

In situ microprobe quantitation of inorganic particle burden in paraffin sections of lung tissue

1988 ◽  
Vol 46 ◽  
pp. 990-991
Author(s):  
Jerrold L. Abraham
Keyword(s):  
Lung Tissue ◽  
Quantitative Information ◽  
Particulate Material ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Qualitative And Quantitative ◽  
The Past ◽  
Quantitative Analyses ◽  
Data Result

Inorganic particulate material of diverse types is present in the ambient and occupational environment, and exposure to such materials is a well recognized cause of some lung disease. To investigate the interaction of inhaled inorganic particulates with the lung it is necessary to obtain quantitative information on the particulate burden of lung tissue in a wide variety of situations. The vast majority of diagnostic and experimental tissue samples (biopsies and autopsies) are fixed with formaldehyde solutions, dehydrated with organic solvents and embedded in paraffin wax. Over the past 16 years, I have attempted to obtain maximal analytical use of such tissue with minimal preparative steps. Unique diagnostic and research data result from both qualitative and quantitative analyses of sections. Most of the data has been related to inhaled inorganic particulates in lungs, but the basic methods are applicable to any tissues. The preparations are primarily designed for SEM use, but they are stable for storage and transport to other laboratories and several other instruments (e.g., for SIMS techniques).

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

scholarly journals Gut Lymph Purification in Rats With Acute Lung Injury Caused By Gut Ischemia Reperfusion Injury

2021 ◽  
Author(s):  
Can Jin ◽  
Shucheng Zhang ◽  
Linlin Wu ◽  
Bohan Li ◽  
Meimei Shi ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Reperfusion Injury ◽  
Lung Tissue ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Mononuclear Phagocyte ◽  
Alveolar Epithelial Cells ◽  
Expression Level ◽  
Tissue Samples

Abstract Rationale: It is unclear whether removing the danger-associated molecular patterns (DAMPs) of gut lymph (GL) in the rats of gut ischemia-reperfusion injury (GIRI) model may reduce the distant organ lung injury.Objective: To determine whether oXiris gut lymph purification (GLP) may remove the DAMPs of GL in the rats’ model of acute lung injury (ALI) caused by GIRI.Methods: The experimental rats were divided into four groups: Sham group, GIRI group, GIRI + gut lymph drainage (GLD) group, and GIRI + GLP group. After successful modeling, the lung tissue samples of rats in each group were taken for hematoxylin-eosin (HE) staining and detection of expression levels of apoptotic indexes. The level of DAMPs was detected in blood and lymph. We observed its microstructure of type II alveolar epithelial cells (AECⅡ), and detected the expression level of apoptosis indexes.Measurements and Main Results: GIRI-induced destruction of alveolar structure, thickened alveolar walls, inflammatory cell infiltration emerged in the GIRI group, HMGB-1 and IL-6 levels significantly increased, and HSP70 and IL-10 levels reduced in lymph and serum. Compared with GIRI group, the lung tissue damage in GIRI + GLP group significantly improved, the expression level of HMGB-1 and IL-6 in the lymph and serum reduced, and HSP70 and IL-10 increased. The organelle structure of AECII in GIRI + GLP group was significantly improved compared with the GIRI group. Conclusions: oXiris GLP blocks the key link between DAMPs and mononuclear phagocyte system to inhibit inflammation and cell apoptosis, thereby reducing ALI induced by GIRI.

Lung tissue volume changes induced by hypertonic NaCl: morphometric evaluation

1981 ◽  
Vol 51 (6) ◽  
pp. 1443-1450 ◽  
Author(s):  
D. Wangensteen ◽  
H. Bachofen ◽  
E. R. Weibel
Keyword(s):  
Lung Tissue ◽  
Alveolar Epithelium ◽  
Cell Types ◽  
Ringer Solution ◽  
Capillary Endothelium ◽  
Vascular Perfusion ◽  
Tissue Samples ◽  
Membrane Area ◽  
Capillary Lumen ◽  
Osmotic Effect

Lung tissue was examined to determine how the volumes of alveolar septum components change when NaCl is added to the vascular perfusate, increasing the osmolarity by 70 mosM. Isolated rabbit lungs were perfused with Ringer solution containing dextran, either with or without added NaCl, and fixed by vascular perfusion. Tissue samples from both “control” and “hypertonic” lungs, prepared for electron microscopy, were examined using established morphometric procedures. Volumes of septal cells, interstitial space, capillary lumen, surface-lining layer, and endothelial and epithelial areas were measured, all normalized against the endothelium basement-membrane area. Results showed that hypertonic NaCl caused a reduction in total cell and surface-lining layer volumes but no change in interstitial or capillary lumen volumes. This supports the hypothesis that small molecules have no osmotic effect across the pulmonary capillary endothelium but do cause a fluid flux from cells and across the alveolar epithelium. Areas and volume measurements for different septal cell types suggest a heterogeneous response: epithelial cells showed significant decreases and endothelial cells changed little, if at all.

scholarly journals Application and Field Validation of a PCR Assay for the Detection ofMycoplasma Hyopneumoniaefrom Swine Lung Tissue Samples

2007 ◽  
Vol 19 (1) ◽  
pp. 91-95 ◽  
Author(s):  
Hugh Y. Cai ◽  
Tony van Dreumel ◽  
Beverly McEwen ◽  
Geoff Hornby ◽  
Patricia Bell-Rogers ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Field Validation ◽  
Pcr Assay ◽  
Tissue Samples

scholarly journals Impact of different stabilization methods on RT-qPCR results using human lung tissue samples

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Margalida Esteva-Socias ◽  
Fernando Gómez-Romano ◽  
José Antonio Carrillo-Ávila ◽  
Alicia Loreto Sánchez-Navarro ◽  
Cristina Villena
Keyword(s):  
Lung Tissue ◽  
Human Lung ◽  
Human Lung Tissue ◽  
Tissue Samples ◽  
Stabilization Methods

Attenuation of hyperoxic lung injury by the 21-aminosteroid U-74389G

1995 ◽  
Vol 78 (5) ◽  
pp. 1635-1641 ◽  
Author(s):  
S. Tasaka ◽  
A. Ishizaka ◽  
T. Urano ◽  
K. Sayama ◽  
F. Sakamaki ◽  
...  
Keyword(s):  
Lung Injury ◽  
Lung Tissue ◽  
Guinea Pigs ◽  
Weight Ratio ◽  
Dry Weight ◽  
Endothelial Damage ◽  
Lung Water ◽  
Tissue Samples ◽  
Cell Accumulation ◽  
Hyperoxic Lung Injury

Hyperoxic lung injury is attributable to oxygen radicals produced under hyperoxic conditions. The 21-aminosteroid (AS), U-74389G, is a potent antioxidant. We examined the effect of U-74389G on lung injury in guinea pigs during exposure to 90% O2 for 48 h. We injected either vehicle or 10 mg/kg of U-74389G 30 min before the O2 exposure and injected the same dose 12, 24, and 36 h later. We performed two series of experiments after exposure. In the first series, we measured the clearance rate of 99mTc-labeled dialdehyde starch (DAS) from the lungs as an index of pulmonary epithelial damage in three experimental groups consisting of 1) control (n = 6) O2 alone (n = 6), and 3) O2 + AS (n = 6). In the second series, pulmonary endothelial injury was estimated by using 28 guinea pigs divided into four experimental groups consisting of 1) control (n = 8), 2) AS alone (n = 5), 3) O2 alone (n = 6), and 4) O2 + AS (n = 9). In the second series, we measured the wet-to-dry weight ratio (W/D) as an index of lung water and the concentration ratio of 125I-labeled albumin in lung tissue and bronchoalveolar lavage (BAL) fluid compared with plasma (T/P and BAL/P, respectively) as indexes of pulmonary endothelial damage. Cell accumulation in BAL fluid and lung tissue samples was also assessed in the second series.(ABSTRACT TRUNCATED AT 250 WORDS)

Rapid Detection of Mycobacterium Tuberculosis in Lung Tissue and Analysis of Drug Resistance

2019 ◽  
Vol 11 (5) ◽  
pp. 728-735
Author(s):  
Junlin Chen ◽  
Fei Huang ◽  
Feifan Xu ◽  
Mei Qu ◽  
Delin Gu ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Dna Sequencing ◽  
Lung Tissue ◽  
Culture System ◽  
Melting Curve ◽  
Gyra Gene ◽  
Tissue Samples ◽  
Curve Method ◽  
Acid Fast Staining

This study investigated the methods for optimizing the workflow for improving the diagnostic efficiency for detection of Mycobacterium tuberculosis (MTB) in lung tissue specimens. A total of 278 specimens were used in this study. M. tuberculosis in fresh lung tissue samples was detected by BACTEC MGIT 960 culture system, culturing L-form MTB, rifampicin (RFP) and levofloxacin (LVFX) susceptibility test, and ribonucleic acid (RNA) simultaneous amplification and testing (SAT). Specimen samples were embedded in paraffin and serially sectioned. The sections were subjected to Ziehl-Neelsen staining and Intensified Kinyoun (IK) acid-fast staining. The suspected MTB or L-form MTB specimens were further investigated by deoxyribonucleic acid (DNA) sequencing and fluorescence polymerase chain reaction (PCR) melting curve method to detect the mutations in rpoB gene and gyrA gene. Thirteen specimens were suspected as MTB positive, and 37 specimens were suspected to be L-form MTB positive by Ziehl-Neelsen staining and IK acid-fast staining. Among the 50 specimens, the number of MTB positive specimens detected by SAT, DNA sequencing, and fluorescence PCR melting curve method was 43, 44, and 45, respectively. Only 11 MTB positive specimens were detected by BACTEC MGIT 960 culture system or by culturing L-form MTB. Mutations detected in rpoB gene and gyrA gene by fluorescence PCR melting curve method were similar to those detected by DNA sequencing. Some specimens, detected by melting curve method, exhibited varied drug resistance to RFP, suggesting heterogeneous resistance. Among the remaining 228 specimens, there was no MTB or L-form MTB detected by BACTEC MGIT 960 culture system. However, 5 specimens were detected to be MTB positive by the SAT method. The fluorescent PCR melting curve method, which has a high level of automation and high sensitivity and specificity, could effectively detect heterozygous drug resistance of MTB in lung tissue samples, which is important for clinicians to effectively formulate a therapeutic strategy.

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