ETHANOL AS A MODIFIER OF RADIATION SENSITIVITY OF LIVING CELLS AGAINST UV-C RADIATION

2019 ◽  
Vol 186 (2-3) ◽  
pp. 191-195
Author(s):  
Barbora Neužilová ◽  
Lukáš Ondrák ◽  
Václav Čuba ◽  
Viliam Múčka
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Dose Rate ◽  
Ethanol Concentration ◽  
Radiation Sensitivity ◽  
Living Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Escherichia Coli Bacteria ◽  
Uv C

ABSTRACT The protection of Escherichia coli bacteria and the yeast Saccharomyces cerevisiae against UV-C radiation by ethanol was studied. It was found that the fraction of surviving cells increases with increasing ethanol concentration. The specific protection depends on the dose rate, concentration range of ethanol, and it is higher for yeast compared to the bacteria.

scholarly journals Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry

LWT ◽  
2011 ◽  
Vol 44 (1) ◽  
pp. 191-198 ◽  
Author(s):  
Marcela Schenk ◽  
Silvia Raffellini ◽  
Sandra Guerrero ◽  
Guillermo A. Blanco ◽  
Stella Maris Alzamora
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Flow Cytometry ◽  
Cell Injury ◽  
Listeria Innocua ◽  
Uv C

scholarly journals Composition of the λ plasmid heritable replicationcomplex

2002 ◽  
Vol 364 (3) ◽  
pp. 857-862 ◽  
Author(s):  
Katarzyna POTRYKUS ◽  
Sylwia BARAŃSKA ◽  
Alicja WĘGRZYN ◽  
Grzegorz WĘGRZYN
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Protein Synthesis ◽  
Plasmid Dna ◽  
Pcr Analysis ◽  
Cross Linking ◽  
Bacteriophage Λ ◽  
Yeast Saccharomyces Cerevisiae ◽  
Replication Complex

Previous studies indicated during replication of plasmids derived from bacteriophage λ (the so-called λ plasmids), that, once assembled, replication complex can be inherited by one of the two daughter plasmid copies after each replication round, and may function in subsequent replication rounds. It seems that similar processes occur during replication of other DNA molecules, including chromosomes of the yeast Saccharomyces cerevisiae. However, apart from some suggestions based on genetic experiments, composition of the λ heritable replication complex remains unknown. In amino acid-starved Escherichia coli relA mutants, replication of λ plasmid DNA is carried out exclusively by the heritable replication complex as assembly of new complexes is impaired due to inhibition of protein synthesis. Here, using a procedure based on in vivo cross-linking, cell lysis, immunoprecipitation with specific sera, de-cross-linking and PCR analysis, we demonstrate that the λ heritable replication complex consists of O, P, DnaB and, perhaps surprisingly, DnaK proteins.

scholarly journals Fermentation of irradiated sugarcane must

2003 ◽  
Vol 60 (4) ◽  
pp. 677-681 ◽  
Author(s):  
André Ricardo Alcarde ◽  
Júlio Marcos Melges Walder ◽  
Jorge Horii
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gamma Radiation ◽  
Dose Rate ◽  
Ethanol Yield ◽  
Ethanolic Fermentation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Treatment ◽  
Yeast Viability ◽  
Volatile Acidity ◽  
Lethal Doses

Bacillus and Lactobacillus are bacteria that usually contaminate the ethanolic fermentation by yeasts and may influence yeast viability. As microorganisms can be killed by ionizing radiation, the efficacy of gamma radiation in reducing the population of certain contaminating bacteria from sugarcane must was examined and, as a consequence, the beneficial effect of lethal doses of radiation on some parameters of yeast-based ethanolic fermentation was verified. Must from sugarcane juice was inoculated with bacteria of the genera Bacillus and Lactobacillus. The contaminated must was irradiated with 2.0, 4.0, 6.0, 8.0 and 10.0 kGy of gamma radiation. After ethanolic fermentation by the yeast (Saccharomyces cerevisiae) the total and volatile acidity produced during the process were evaluated; yeast viability and ethanol yield were also recorded. Treatments of gamma radiation reduced the population of the contaminating bacteria in the sugarcane must. The acidity produced during the fermentation decreased as the dose rate of radiation increased. Conversely, the yeast viability increased as the dose rate of radiation increased. Gamma irradiation was an efficient treatment to decontaminate the must and improved its parameters related to ethanolic fermentation, including ethanol yield, which increased 1.9%.

scholarly journals A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53.

1996 ◽  
Vol 16 (12) ◽  
pp. 7133-7143 ◽  
Author(s):  
D S Lim ◽  
P Hasty
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Cell Death ◽  
Double Mutant ◽  
Radiation Sensitivity ◽  
Chromosome Loss ◽  
Recombinational Repair ◽  
Embryonic Lethal

RecA in Escherichia coli and its homolog, ScRad51 in Saccharomyces cerevisiae, are known to be essential for recombinational repair. The homolog of RecA and ScRad51 in mice, MmRad51, was mutated to determine its function. Mutant embryos arrested early during development. A decrease in cell proliferation, followed by programmed cell death and chromosome loss, was observed. Radiation sensitivity was demonstrated in trophectoderm-derived cells. Interestingly, embryonic development progressed further in a p53 null background; however, fibroblasts derived from double-mutant embryos failed to proliferate in tissue culture.

scholarly journals Saccharomyces cerevisiae displays an increased growth rate and an extended replicative lifespan when grown under respiratory conditions in the presence of bacteria

2017 ◽  
Vol 63 (9) ◽  
pp. 806-810
Author(s):  
Paul A. Kirchman ◽  
Nicholas Van Zee
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Volatile Compound ◽  
Lifespan Extension ◽  
Yeast Saccharomyces Cerevisiae ◽  
Replicative Lifespan ◽  
Solid Media ◽  
Respiratory Conditions ◽  
Ferment Glucose

Individual cells of the budding yeast Saccharomyces cerevisiae have a limited replicative potential, referred to as the replicative lifespan. We have found that both the growth rate and average replicative lifespan of S. cerevisiae cells are greatly increased in the presence of a variety of bacteria. The growth and lifespan effects are not observable when yeast are allowed to ferment glucose but are only notable on solid media when yeast are forced to respire due to the lack of a fermentable carbon source. Growth near strains of Escherichia coli containing deletions of genes needed for the production of compounds used for quorum sensing or for the production of the siderophore enterobactin also still induced the lifespan extension in yeast. Furthermore, the bacterially induced increases in growth rate and lifespan occur even across gaps in the growth medium, indicating that the bacteria are influencing the yeast through the action of a volatile compound.

Isolation of Saccharomyces cerevisiae YDJ05 from the Spontaneous Fermentation Pear Wine and Study of the Yeast Growth Dynamics during the Association Fermentation

2010 ◽  
Vol 156-157 ◽  
pp. 266-271
Author(s):  
Da Wei Zhang ◽  
Wenbin Dong ◽  
Lei Jin ◽  
Jie Zhang ◽  
Yuan Chang Jin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Growth Dynamics ◽  
Yeast Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Fermentation ◽  
Potential Source ◽  
Yeast Strains ◽  
Better Than

Five preponderant yeast strains (YDJ01, YDJ02, YDJ03, YDJ04 and YDJ05) were isolated from the spontaneous fermentation pear wine as source of yeast for wine making from pear. Ethanol yield of YDJ05 was the highest and its using rapidity of the sugar was the most quickly. YDJ05 was identified as Saccharomyces cerevisiae and named Saccharomyces cerevisiae YDJ05. In addition, the fermentation dynamics of three yeast strains (Saccharomyces cerevisiae YDJ05, “Angle” yeast and Saccharomyces cerevisiae GIM2.39) were studied including single fermentation and associated fermentation. The fermentative behavior of three strains changed in association fermentations (Saccharomyces cerevisiae YDJ05 and “Angle” yeast, Saccharomyces cerevisiae YDJ05 and Saccharomyces cerevisiae GIM2.39). Results indicated that the qualities of pear wines made from association fermentations were better than that of single fermentations. The pear wine fermented associated by Saccharomyces cerevisiae YDJ05 and Saccharomyces cerevisiae GIM2.39 was the best in quality by sensory evaluation among all pear wines whose ethanol concentration was 10.3% (v/v). Saccharomyces cerevisiae YDJ05 and mai could be excellent potential source of strains.

scholarly journals PRION PROTEIN AND AMYLOID BETA PEPTIDE INTERACT IN THE YEAST SACCHAROMYCES CEREVISIAE

2012 ◽  
Vol 10 (1) ◽  
pp. 74-80
Author(s):  
Aleksander A. Rubel ◽  
Viktoria V. Korzhova ◽  
Alsu F. Saifitdinova ◽  
Kirill S. Antonez ◽  
Sergey G. Inge-Vechtomov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescence Microscopy ◽  
Prion Protein ◽  
Amyloid Beta ◽  
Living Cells ◽  
Yeast Cells ◽  
Amyloid Beta Peptide ◽  
Yeast Saccharomyces Cerevisiae ◽  
3D Microscopy ◽  
Beta Peptide

SUMMARY: The possibility of interaction between Prion Protein and amyloid beta peptide in living cells of yeast Saccharomyces cerevisiae have been investigated by fluorescence 3D microscopy. Using the FR ET technique, it was shown that amyloid beta peptide and PrP interact in yeast cells. In the future, the yeast model can be used for investigation of the fine mechanisms of this interaction by fluorescence microscopy.

scholarly journals Research of the inactivation of pathogens in water under exposure to low temperature plasma

2019 ◽  
Vol 95 (6) ◽  
pp. 588-592 ◽  
Author(s):  
V. A. Kolesnikov ◽  
Roman Yakushin ◽  
V. A. Brodsky ◽  
E. S. Babusenko ◽  
A. V. Chistolinov
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Bacillus Subtilis ◽  
Low Temperature ◽  
Detrimental Effect ◽  
Coliform Bacteria ◽  
Low Temperature Plasma ◽  
Yeast Saccharomyces Cerevisiae ◽  
Temperature Plasma ◽  
General Decline

There was investigated the effect of barrier and spark discharge low temperature plasma on water containing the cells of Escherichia coli (Escherichia coli), hay bacillus (Bacillus subtilis) and yeast (Saccharomyces cerevisiae). There was shown a general decline in the concentration of viable microbial cells after the treatment of suspensions. There was especially marked the detrimental effect of the method on the viability of sanitary-indicative coliform bacteria in the water.

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