scholarly journals Assessment of an iPad-based Sperm Motility Analyzer for Determination of Canine Sperm Motility

2021 ◽  
Author(s):  
Evelyn Bulkeley ◽  
Christine Collins ◽  
Azarene Foutouhi ◽  
Kris Gonzales ◽  
Heather Power ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Alternative Measurement ◽  
Positive Correlations ◽  
Casa System ◽  
Progressive Assessment

Abstract The objective of this study was to evaluate the repeatability and accuracy of canine sperm motility (total and progressive) assessment with a tablet-based Canine iSperm ® instrument compared to computer-assisted sperm analysis (CASA). The experiment used fresh and frozen/thawed canine semen samples for comparisons of semen analysis parameters (concentration, total motility, and progressive motility) between a CASA system, iSperm ®, and NucleoCounter ® SP-100 ™ (concentration) instruments. Spearman’s Rho correlational analysis was used to identify significant associations between motility assessment methods. Significant positive correlations were found between CASA assessment and iSperm ® for both progressive and total motility measurements. We also determined the coefficient of variation (CV) for repeatability of sample analysis for iSperm ® and CASA for fresh sperm, wherein each sample was assessed 10 times on both devices. For fresh and frozen-thawed samples, concentration assessment by iSperm ® showed high variability (CV= 19.9 ± 1.5%). For iSperm ® assessment of total and progressive motility, the CV’s were 6.3 ± 0.5% and 10.7 ± 0.8%, respectively. The results indicate that the iSperm ® application offers an accurate and alternative measurement of motility to traditional CASA analysis, though caution should be taken when assessing concentration due to the high CV observed in this study.

162 FREEZING OF SEMEN FROM ENDANGERED ASTURIANA DE LA MONTAÑA BULLS IN ZWITTERONIC LIPIDS-BASED EXTENDERS

2008 ◽  
Vol 20 (1) ◽  
pp. 161 ◽  
Author(s):  
C. Tamargo Miguel ◽  
S. S. Pérez-Garnelo ◽  
P. Beltrán Breña ◽  
A. T. Palasz ◽  
J. De la Fuente ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Analysis ◽  
Egg Yolk ◽  
Semen Parameters ◽  
Computer Assisted ◽  
Progressive Motility ◽  
Bull Semen ◽  
Straight Line ◽  
Before And After ◽  
Arcsine Transformation

This experiment was designed to test the efficacy of 2 different preparation protocols of zwitteronic soyabean-origin lipids for the production of lipidsglycerol liposomes for use in bull semen cryopreservation. Lipids liposomes were prepared at 10% concentration in Tris buffer by 1. highpressure homogenization (Panda 2K, Parma, Italy) and then 8% glycerol were added, extender-1 (E-1); Lipids were homogenized together with glycerol, extender-2 (E-2). Bioxcell extender (E-3) was used as control. Semen was collected 3 times from 3 endangered Asturiana de la Monta�a bulls by the means of an artificial vagina. Ejaculates with at least 70% motility were processed further by a standard freezing protocol used in our AI station. Semen was diluted at 37�C with each of the 4 extenders to a concentration of 92 � 106 spermatozoa per mL, cooled to 4�C over 4 h, aspirated into 0.25-mL plastic straws (IMV Technologies, Aigle, France), frozen in a bio-freezer (IMV Technologies) in 3 steps from 4 to –140�C, and then plunged into liquid N2. Straws were thawed in a water bath at 37�C for 30 s. Sperm motility was analyzed microscopically immediately after collection, after dilution, and after 4, 24, 48, and 72 h of storage at 4�C. Post-thaw semen progressive motility was assessed microscopically, and sperm movement characteristics were analyzed by computer-assisted semen analysis (CASA) (SCA�, Microptic, Barcelona, Spain). Data were compared between extenders and bulls by 2-way ANOVA; percentages were transformed by arcsine transformation before ANOVA. Total and progressive sperm motility at 0 h after dilution ranged from 90 to 70% and was not different between extenders and bulls. There was no difference between bulls in total and progressive motility after 24 h of cold storage; however, both were significantly greater (P < 0.05) for Control (62.4 � 14.7 and 41.4 � 14.9) and E-1 (70.1 � 12 and 33.8 � 7.0) extenders than for the E-2 extender (22.5 � 17 and 1.2 � 1.3). Average post-thaw sperm motility was not different between bulls for either extender, but motility for Bioxcell (Control, 48.1 � 14.6%) and E-1 extenders (43.2 � 13.0%) were significantly greater (P < 0.05) than for E-2 extender (18.7 � 8.8%). There were no differences between bulls for all kinematic semen parameters: curvilinear (VCL), straight line (VSL), average path (VAP) velocities, linearity (LIN) and straightness (STR), evaluated by CASA before and after freezing; however, all were lower (P < 0.05) for the E-2 extender and not different between Control and E-1 extenders. Sperm movements derived from heads (VCL) and linearity of sperm(LIN), both closely related to field fertility, were in the range of 90.9 � 2.1 and 63.0 � 5.5 for E-3 (Control) extender; 99.1 � 3.4 and 49.4 � 3.5 for E-1; and 21.8 � 2.2 and 29.9 � 4.0 for E-2. In summary, zwitteronic soyabean lipid liposomes are an effective egg yolk substitute for the cryopreservation of Asturiana de la Monta�a bull semen; however, the homogenization protocol of the lipids-glycerol mixture must be improved.

55 EFFECT OF TREHALOSE FOR FROZEN–THAWED RAM SPERM MOTILITY PARAMETERS

2015 ◽  
Vol 27 (1) ◽  
pp. 120
Author(s):  
M. M. Toishibekov ◽  
M. T. Jazkbayev ◽  
B. B. Molzhigitov
Keyword(s):  
Sperm Motility ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Computer Assisted ◽  
Progressive Motility ◽  
Indigenous Sheep ◽  
Standard Tool ◽  
Freezing Curve ◽  
Ram Sperm

Computer-assisted sperm analysers have become the standard tool for evaluating sperm motility because they provide objective results for thousands of mammalian spermatozoa. Ram semen was collected using electro-ejaculation from 10 adult rams of Chingizskaya indigenous sheep breed. Motility was determined using computer-automated semen analysis (Hamilton Thorne Motility Analyzer, Beverly, MA, USA). Trehalose solution (0.375 M) was added to Tris-buffered saline solution to give the following trehalose extenders: 25, 50, 75, and 100% (vol:vol), and analysed for motility using computer-automated semen analysis. The sperm pellets were resuspended at 24°C in cooling extender – trehalose extenders of each concentration containing 5% egg yolk. The diluted semen was cooled to 5°C within 2 h. The semen was then further diluted 1 : 1 with freezing extender – each trehalose extender containing 1.5% glycerol to obtain a sperm concentration of 2.0 × 108 cells mL–1 – and then loaded into 0.5-mL straws. Straws were frozen using a programmable freezer with a freezing curve of 5°C to –5°C at 4°C per min, –5°C to –110°C at 25°C per min, and –110°C to –140°C at 35°C per min, and then the straws were plunged into liquid nitrogen for storage. Frozen samples were thawed in a 37°C water bath for 30 s and analysed for motility using computer-automated semen analysis. Statistical analyses were performed with a Student's test. The fresh semen samples showed the next results: motility 88.3 ± 2.4%, progressive motility 26.8 ± 6.9%, and progressive velocity 61.9 ± 4.2 μm s–1. Motility of the frozen-thawed spermatozoa was 63.6 ± 2.9% (25% trehalose), 55.6 ± 5.2% (50%), 32.4 ± 4.7% (75%), and 23.6 ± 3.2 (100%). Progressive motility was 15.6 ± 3.9% (25%), 13.7 ± 3.7% (50%), 4.5 ± 1.3% (75%), and 5.2 ± 1.3% (100%). Progressive velocity was 93.5 ± 8.3 μm s–1 (25%), 85.4 ± 8.1 μm s–1 (50%), 65.7 ± 6.1 μm s–1 (75%), 35.2 ± 3.3 μm s–1 (100%). Motility of the frozen-thawed spermatozoa significantly decreased with increasing concentrations of trehalose in the extender (P < 0.05). These preliminary studies showed that further research is needed of use trehalose for ram spermatozoa cryoconservation.

scholarly journals 448 Late-breaking: GWAS analysis show QTL in horses which are characterized by sperm resistance to freezing

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 119-120
Author(s):  
Andrei A Kudinov ◽  
Natalia Dementieva ◽  
Elena Nikitkina ◽  
Michail Atroshchenko ◽  
Artem Musidray
Keyword(s):  
Sperm Motility ◽  
Association Studies ◽  
Semen Analysis ◽  
Individual Variability ◽  
Mitochondrial Activity ◽  
Computer Assisted ◽  
Russian Science ◽  
Progressive Motility ◽  
Genome Wide ◽  
Selection Of

Abstract Damaging effects of low temperatures on the functionality of sperm is well known. Stallion sperm cryoresistance has a high individual variability. The genetic basis of cryoresistance and its heritability are little studied. The aim of the study was to search for genome-wide associations with sperm motility after freezing. 96 sperm samples were collected. The collected sperm was diluted to a final concentration 100 mln/ml and frozen in 0.5 ml straws or 18–20 ml tubes. The evaluation of semen was carried out no earlier than 24 hours after freezing. Sperm motility was assessed by computer-assisted semen analysis (CASA). Genomic DNA was purified form semen samples and genotyped using Affymetrix Equine HD array. Genotypes quality control and association studies were performed in Plink 1.9 and EMMAX software respectively. Evaluation of sperm motility showed high individual variability in both total and progressive motility after freezing. Total motility varied from 0.5 to 92.5%, and progressive motility from 0.5 to 70.8%. Sufficient associations of PTF with SNP’s were detected on chromosome (Chr) 1 (P &lt; 1.33e-09) and 4 (P &lt; 2.00e-09). Found SNP’s were located in genes PAS domain-containing protein 3 (Chr1) and UBAP1-MVB12 (Chr4). Expression of these genes in human body was found in cerebrum and male genitals. Suggestive SNPs were found lying nearby to genes responsible for formation of cell wall and proteins affecting mitochondrial activity. Performed studies are presenting first step of understanding genetic background of cryoresistancy of semen in horses. Found markers could be used for selection of stallions based on cryopreservation ability. Authors acknowledge financial support from Russian Science Foundation, Grant No: 18-16-00071.

scholarly journals Liquid Storage of Boar Semen Using Commercial Extenders

2018 ◽  
Vol 75 (1) ◽  
pp. 66
Author(s):  
Liviu BOGDAN ◽  
Mihai CENARIU ◽  
Mihai BORZAN ◽  
Simona CIUPE ◽  
Lehel SZABO ◽  
...  
Keyword(s):  
Semen Parameters ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Before And After ◽  
Boar Semen ◽  
Casa System ◽  
Sexually Mature ◽  
Term Storage

The research was focused on the modern evaluation of boar semen parameters, using computer assisted sperm analysis (CASA), before and after liquid storage at 15ºC. Semen was collected from 15 sexually mature boars by manual stimulation. Macroscopical and microscopical evaluation of semen was performed, followed by a detailed CASA analysis of all ejaculates. Subsequently, semen was diluted using 4 different extenders (Semtest, Androstar, MIII and Cronos) and stored at 15ºC for 24 hours. Next, evaluation of progressive motility, total motility and viability was performed, using the same CASA system. All experiments were performed in triplicates and results were statistically analyzed. The average progressive motility after 24 hours was as follows: 69.56 ± 6.38 for MIII, 65.92% ± 2.63 for Semtest, 67.07% ± 5.58 for Androstar Plus and 68.93% ± 3.40 for Cronos. The viability results after 24 hours were: 86.34% ± 1.38 for Semtest extender, 93.55% ± 3.38% for Androstrar Plus, 89.19% ± 3.42 for MIII and 91.35% ± 2.37 for Cronos. The findings of this study suggest that the use of commercial extenders for short-term storage of swine semen is important in order to increase sperm longevity with minimal sperm function deterioration.

scholarly journals Technical Note: The use of iSperm technology for on-farm measurement of equine sperm motility and concentration

2019 ◽  
Vol 3 (4) ◽  
pp. 1513-1520
Author(s):  
Christa R Moraes ◽  
Erin E Runcan ◽  
Bryan Blawut ◽  
Marco A Coutinho da Silva
Keyword(s):  
Sperm Motility ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Technical Note ◽  
Computer Assisted ◽  
Analysis Tool ◽  
Velocity Measurements ◽  
Perfect Agreement ◽  
Sperm Analysis ◽  
On Farm

Abstract The iSperm is a newly released semen analysis tool from Aidmics Biotechnology Co. LTD, which allows an iPad Mini to be transformed into a handheld microscope with objective semen analysis software for equine available through the Apple Store (version 4.5.2). The aim of this study was to compare iSperm values for sperm motility and sperm concentration to current acceptable methods for semen analysis and to determine the agreement with these methods using statistical methods. Two ejaculates from each of five Standardbred stallions were used to compare sperm motility (computer-assisted semen analysis [CASA] vs. iSperm) and concentration (NucleoCounter SP-100 [NC] vs. hemocytometer vs. iSperm). Data were analyzed by first testing for the differences between the means of each method using a linear mixed-effects model. The agreement between the two continuous measurements for each method was then investigated by computing Lin’s concordance correlation coefficient (CCC), with a value of 1 indicating perfect agreement between methods. Results are reported as the CCC with the associated 95% confidence interval in parentheses. Means for both total motility (TM) and progressive motility (PM) were equal between CASA and iSperm values (P = 0.0741 and P = 0.725, respectively). However, means for all velocity measurements were significantly different between CASA and iSperm readings (P &lt; 0.001). For concentration, means were equal between NC and iSperm values (P = 0.748) and for hemocytometer and iSperm values (P = 0.953). The CCC for TM was 0.871 (0.788, 0.923) and for PM was 0.916 (0.847, 0.955) indicating good agreement between methods. Low levels of agreement were observed for all velocity measurements. Finally, the CCC for concentration compared by iSperm and NC was 0.970 (0.949, 0.982) and for iSperm and hemocytometer it was 0.962 (0.934, 0.978), both close to the line of perfect concordance. Although more work is needed to improve the iSperm software for velocity measurements to be acceptable by research standards, in its present form the iSperm will introduce a low-cost and affordable method for on-farm semen analysis (TM, PM, concentration) for breeders and veterinarians. As a result, more farms will have access to accurate sperm analysis tools which will help to standardize semen processing procedures leading to better overall quality of semen used for artificial insemination.

76 COMPUTER-ASSISTED SPERM ANALYSIS OF FROZEN SPERM MOTILITY AFTER DIFFERENT TIMES OF EXPOSURE AT 15°C

2008 ◽  
Vol 20 (1) ◽  
pp. 119
Author(s):  
A. Garcia Guerra ◽  
M. P. Etcheverry ◽  
D. Rodriguez ◽  
G. Larraburu ◽  
G. M. Brogliatti
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Room Temperature ◽  
Cell Damage ◽  
Semen Analysis ◽  
Beat Frequency ◽  
Computer Assisted ◽  
Frozen Semen ◽  
Significant Difference ◽  
Casa System

One of the key factors for successful long-term cryopreservation in liquid nitrogen is maintaining the samples at –130°C or lower at all times to avoid cell damage (Barth 1991 Proc. 10th Ann. Conv. Am. Embr. Transf. Assoc., 20–26). Previous data indicated that exposure of the semen straw to ambient temperature for more than 15 s can raise the temperature above –130°C and reduce sperm motility, as determined by subjective evaluation (Berndtson et al. 1976 Proc. 6th NAAB Tech. Conf. Artif. Insem. Reprod., 51–60). The computer-assisted semen analysis (CASA) system provides an opportunity to assess multiple motility characteristics on a semen sample objectively and with high repeatability. An experiment was designed to evaluate the effect of exposing frozen semen in 0.5-mL straws to room temperature for 15, 30, 60, or 120 s on motility characteristics assessed by CASA system. Twenty-eight ejaculates from different bulls (19 Angus, 7 Hereford, 1 Brangus, 1 Shorthorn) were diluted using a chemically semi-defined media (Andromed, Minitüb, Tiefenbach, Germany) and frozen in an automatic freezer (Digicool, IMV, Paillette Crista, France). Five frozen straws per bull were used, one for each time of exposure and one as control (0 s = 0 time). Straws were exposed to room temperature (15°C ± 0.78) for different times and then placed back into liquid nitrogen. Semen thawing was conducted in a water bath at 37°C for 1 min. Motility characteristics were evaluated by the IVOS SpermAnalyzer (Hamilton Thorne Research, Beverly, MA, USA). Two chambers of 20-μm depth and 5 fields per chamber were analyzed (30 frames/0.5 s for each field). Seven motility parameters were evaluated: motile sperm (%), progressive sperm (%), VAP (path velocity, μm s–1), VCL (track speed, μm s–1), ALH (lateral amplitude, μm), BCF (beat frequency, Hz), and LIN (Linearity, %). The Kruskal–Wallis test was used to compare variables among groups, and results are shown in Table 1. There is a significant difference (P < 0.05) in the % of motile and progressive sperm when time of exposure was increased. There was a drastic and significant reduction in the percentage of motile and progressive sperm when exposure to 15°C was longer than 30 s. The live cells had similar motile characteristics: VAP, VCL, ALH, BCF, and LIN. In conclusion, sperm motility would be affected if straws are exposed for more than 30 s. More research should be done to test higher room temperatures, detect viability effects, and determine pregnancy rates after AI. Table 1. CASA of frozen sperm motility characteristics at different times of exposure at 15°C This research was supported by Centro Genetico Bovino Eolia S.A.

39 General motility and mitochondrial cytochemical activity of post-thawed semen of pasture-fed Nelore bulls supplemented with palm and soybean oils

2019 ◽  
Vol 31 (1) ◽  
pp. 145
Author(s):  
P. P. Tsuneda ◽  
L. K. Hatamoto-Zervoudakis ◽  
T. F. Motheo ◽  
J. T. Zervoudakis ◽  
M. Nichi
Keyword(s):  
Sperm Motility ◽  
Lateral Displacement ◽  
Young Male ◽  
Mitochondrial Activity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Activity Class ◽  
Sperm Analysis ◽  
Soybean Oils ◽  
Progressive Motility

This study aimed to evaluate general motility and mitochondrial cytochemical activity of post-thawed semen of pasture-fed Nelore bulls supplemented with palm and soybean oils (protected by calcium salts). Twenty-four young male Nellore bulls were randomly assigned to 2 groups: control (CO; corn supplement and distillery grains without the addition of protected fat) and palm and soybean oils association (OP+OS; control supplement+145g of protected soybean oil+145g of protected palm). The experiment lasted 84 days, and semen was collected for cryopreservation. Post-thawed general sperm motility was assessed by computer-assisted sperm analysis using a computerized analyser (HTR Ivos II; Hamilton Thorne, Beverly, MA, USA). Motility (%), progressive motility (%), velocity average path (μm s−1), linearity (%), and lateral displacement of sperm head (μm) were analysed. Additionally, mitochondrial cytochemical activity was evaluated by co-incubation with 3,3′-diaminobenzidine (DAB; 1mg mL−1 of PBS) at 37°C (water bath) for 1h under reduced lighting conditions. Sperm cells were classified based on the mitochondrial activity of their midpiece in a 4-class scale: class 1 (DAB 1; all mitochondria are active; sperm cells with midpiece completely stained), class 2 (DAB 2; sperm cells with active and inactive segments but with prevalence of active stained segments, indicating average to high mitochondrial activity), class 3 (DAB 3; spermatozoa with less than half of the mitochondrial sheath active), and class 4 (DAB 4; spermatozoa totally inactive, with midpiece completely discolored). Data were analysed using SAS statistical program (SAS 9.3, SAS Institute Inc., Cary, NC, USA), and Tukey’s test was used to identify treatments. Study design was completely randomized, and effects were declared significant at P&lt;0.05. Increased percentage of sperm motility (CO: 30.27±5.44v. OP+OS: 38.90±4.68) and progressive motility (CO: 17.27±3.84v. OP+OS: 22.90±3.88) were noticed in post-thawed samples after supplementation. Nevertheless, the addition of palm and soybean oils to the feed promoted a decrease in the percentage of DAB 3 sperm cells (CO: 4.77±1.03v. OP+OS: 2.06±0.51; P=0.0267). Therefore, supplementation with palm and soybean oils improves sperm kinetics in post-thawed semen of grazing Nellore bulls.

scholarly journals Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele

2016 ◽  
Vol 88 (4) ◽  
pp. 279 ◽  
Author(s):  
Filomena Scarselli ◽  
Anna Maria Lobascio ◽  
Mario Terribile ◽  
Valentina Casciani ◽  
Pierfrancesco Greco ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
High Viscosity ◽  
Control Group ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Spermatozoa Motility ◽  
Progressive Motility ◽  
Significant Difference ◽  
Casa System ◽  
Phosphate Buffered Saline

The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients’ ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration &gt; 2 million/ml and progressive motility &lt; 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS). Afterwards, the sperm progressive motility was assessed using Computer Assisted Sperm Analysis (CASA system). Incubation with MYO-Inositol improved sperm progressive motility in high viscosity samples compared to control group (38.9% ± 3.0 vs 24.35% ± 2.41, respectively; p ≤ 0.0001). Conversely, no statistically significant difference was observed in total sperm progressive motility in varicocele samples compared with control group (22.7% ± 2.07 vs 26.7% ± 3.31, respectively; p = 0.085). The MYO-Inositol positive effect on spermatozoa motility may depend on the type of sperm damage: heavy structural and biochemical defects which typically affects patients with varicocele are not restored by Inositol. On the contrary, MYOInositol is able to improve sperm motility in semen samples with high viscosity, since those samples show no substantial structural sperm defects.

scholarly journals Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

2021 ◽  
Vol 50 (5) ◽  
Author(s):  
Ngcauzele Ngcauzele ◽  
G. Van der Horst ◽  
A. Kotze ◽  
T. Jonker ◽  
L. Maree
Keyword(s):  
Sperm Motility ◽  
Animal Species ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Procaine Hydrochloride ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Sperm Morphometry ◽  
Fertility Potential ◽  
Phosphate Buffered Saline

Computer-assisted semen analysis (CASA) is an automated and objective method of evaluating structural (e.g. morphology) and functional sperm parameters (e.g. motility and hyperactivation). Sperm hyperactivation is essential for successful fertilization and is thus an important aspect in determining the fertility potential of a male. In the current study, CASA was used for standard semen analysis and for comparison of the ability of phosphate buffered saline (PBS), BO sperm wash (10 mM caffeine), 4% lignocaine, and 5 mM procaine hydrochloride to induce hyperactivation in Tankwa goat spermatozoa. Twenty-nine ejaculates were collected from randomly selected male goats by electroejaculation. Although none of the four media affected percentage total sperm motility, lignocaine caused a significant decrease (P >0.05) in percentage progressive motility. Exposure to procaine resulted in an increase in swimming speed (P ≤0.05) and star-spin motility tracks, which are typical of sperm hyperactivation. Using PBS and procaine motility data from individually selected spermatozoa, receiver operator characteristic curves were constructed to distinguish the kinematic parameters employed as cut-off values for sperm hyperactivation. PBS and BO sperm wash did not induce hyperactivation (0.1 + 0.2% and 0.04  0.2% respectively), while lignocaine induced little hyperactivation (3.4 + 3.0%) and procaine hydrochloride had the highest percentage hyperactivation (25.3 + 13.6%). The large variation in hyperactivation (0–54.5%) may reflect inter-individual differences in sperm quality among these males. This study indicated procaine hydrochloride was the most promising hyperactivation-inducing medium for Tankwa goat spermatozoa and should be considered for similar assessments in other animal species Keywords: computer-aided sperm analysis, procaine hydrochloride, sperm kinematics, sperm morphometry, sperm motility

Sign in / Sign up

Export Citation Format

Share Document