scholarly journals Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis

2009 ◽  
Vol 22 (4) ◽  
pp. 456-468 ◽  
Author(s):  
E. Jourdan ◽  
G. Henry ◽  
F. Duby ◽  
J. Dommes ◽  
J. P. Barthélemy ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cyclic Peptide ◽  
Induced Defense ◽  
Plant Cells ◽  
Defense Responses ◽  
Growth Potential ◽  
Systemic Resistance ◽  
Extracellular Medium ◽  
Molecular Events ◽  
Bacillus Spp

Multiple strains of Bacillus subtilis were demonstrated to stimulate plant defense responses, and cyclic lipopeptides may be involved in the elicitation of this induced systemic resistance phenomenon. Here, we further investigated molecular events underlying the interaction between such lipopeptides and plant cells. Addition of surfactin but not fengycin or iturin in the micromolar range to tobacco cell suspensions induced defense-related early events such as extracellular medium alkalinization coupled with ion fluxes and reactive oxygen species production. Surfactin also stimulated the defense enzymes phenylalanine ammonia lyase and lipoxygenase and modified the pattern of phenolics produced by the elicited cells. The occurrence of these surfactin-elicited early events is closely related to Ca2+ influx and dynamic changes in protein phosphorylation but is not associated with any marked phytotoxicity or adverse effect on the integrity and growth potential of the treated tobacco cells. Reduced activity of some homologues also indicates that surfactin perception is dictated by structural clues in both the acyl moiety and cyclic peptide part. Our results suggest that these molecules could interact without irreversible pore formation but in a way sufficient to induce disturbance or transient channeling in the plasma membrane that can, in turn, activate a biochemical cascade of molecular events leading to defensive responses. The present study sheds new light not only on defense-related events induced following recognition of amphiphilic lipopeptides from Bacillus spp. but also more globally on the way elicitors from beneficial bacteria can be perceived by host plant cells.

scholarly journals Bacillus subtilis and Pseudomonas fluorescens Trigger Common and Distinct Systemic Immune Responses in Arabidopsis thaliana Depending on the Pathogen Lifestyle

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 503
Author(s):  
Ngoc Huu Nguyen ◽  
Patricia Trotel-Aziz ◽  
Sandra Villaume ◽  
Fanja Rabenoelina ◽  
Adrian Schwarzenberg ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Immune Responses ◽  
Signaling Pathways ◽  
Pseudomonas Syringae ◽  
Systemic Resistance ◽  
Functional Study ◽  
Marker Genes ◽  
Beneficial Bacteria ◽  
Bacillus Spp ◽  
Immune Pathways

Plants harbor various beneficial bacteria that modulate their innate immunity, resulting in induced systemic resistance (ISR) against various pathogens. However, the immune mechanisms underlying ISR triggered by Bacillus spp. and Pseudomonas spp. against pathogens with different lifestyles are not yet clearly elucidated. Here, we show that root drenching of Arabidopsis plants with Pseudomonas fluorescensPTA-CT2 and Bacillus subtilis PTA-271 can induce ISR against the necrotrophic fungus B. cinerea and the hemibiotrophic bacterium Pseudomonas syringae Pst DC3000. In the absence of pathogen infection, both beneficial bacteria do not induce any consistent change in systemic immune responses. However, ISR relies on priming faster and robust expression of marker genes for the salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) signaling pathways upon pathogen challenge. These responses are also associated with increased levels of SA, JA, and abscisic acid (ABA) in the leaves of bacterized plants after infection. The functional study also points at priming of the JA/ET and NPR1-dependent defenses as prioritized immune pathways in ISR induced by both beneficial bacteria against B. cinerea. However, B. subtilis-triggered ISR against Pst DC3000 is dependent on SA, JA/ET, and NPR1 pathways, whereas P. fluorescens-induced ISR requires JA/ET and NPR1 signaling pathways. The use of ABA-insensitive mutants also pointed out the crucial role of ABA signaling, but not ABA concentration, along with JA/ET signaling in primed systemic immunity by beneficial bacteria against Pst DC3000, but not against B. cinerea. These results clearly indicate that ISR is linked to priming plants for enhanced common and distinct immune pathways depending on the beneficial strain and the pathogen lifestyle.

scholarly journals Sulfated Fucan Oligosaccharides Elicit Defense Responses in Tobacco and Local and Systemic Resistance Against Tobacco Mosaic Virus

2003 ◽  
Vol 16 (2) ◽  
pp. 115-122 ◽  
Author(s):  
Olivier Klarzynski ◽  
Valérie Descamps ◽  
Bertrand Plesse ◽  
Jean-Claude Yvin ◽  
Bernard Kloareg ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Extracellular Medium ◽  
Sulfated Fucan ◽  
Strong Stimulation ◽  
Local And Systemic Resistance

Sulfated fucans are common structural components of the cell walls of marine brown algae. Using a fucan-degrading hydrolase isolated from a marine bacterium, we prepared sulfated fucan oligosaccharides made of mono- and disulfated fucose units alternatively bound by α-1,4 and α-1,3 glycosidic linkages, respectively. Here, we report on the elicitor activity of such fucan oligosaccharide preparations in tobacco. In suspension cell cultures, oligofucans at the dose of 200 μg ml−1 rapidly induced a marked alkalinization of the extracellular medium and the release of hydrogen peroxide. This was followed within a few hours by a strong stimulation of phenylalanine ammonia-lyase and lipoxygenase activities. Tobacco leaves treated with oligofucans locally accumulated salicylic acid (SA) and the phytoalexin scopoletin and expressed several pathogenesis-related (PR) proteins, but they displayed no symptoms of cell death. Fucan oligosaccharides also induced the systemic accumulation of SA and the acidic PR protein PR-1, two markers of systemic acquired resistance (SAR). Consistently, fucan oligosaccharides strongly stimulated both local and systemic resistance to tobacco mosaic virus (TMV). The use of transgenic plants unable to accumulate SA indicated that, as in the SAR primed by TMV, SA is required for the establishment of oligofucan-induced resistance.

scholarly journals Arabidopsis RETICULON-LIKE4 (RTNLB4) Protein Participates in Agrobacterium Infection and VirB2 Peptide-Induced Plant Defense Response

2020 ◽  
Vol 21 (5) ◽  
pp. 1722 ◽  
Author(s):  
Fan-Chen Huang ◽  
Hau-Hsuan Hwang
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Induced Defense ◽  
Plant Cells ◽  
Defense Responses ◽  
Infection Process ◽  
Wild Type ◽  
Type Iv ◽  
Induced Defense Responses ◽  
Peptide Treatment

Agrobacterium tumefaciens uses the type IV secretion system, which consists of VirB1-B11 and VirD4 proteins, to deliver effectors into plant cells. The effectors manipulate plant proteins to assist in T-DNA transfer, integration, and expression in plant cells. The Arabidopsis reticulon-like (RTNLB) proteins are located in the endoplasmic reticulum and are involved in endomembrane trafficking in plant cells. The rtnlb4 mutants were recalcitrant to A. tumefaciens infection, but overexpression of RTNLB4 in transgenic plants resulted in hypersusceptibility to A. tumefaciens transformation, which suggests the involvement of RTNLB4 in A. tumefaciens infection. The expression of defense-related genes, including FRK1, PR1, WRKY22, and WRKY29, were less induced in RTNLB4 overexpression (O/E) transgenic plants after A. tumefaciens elf18 peptide treatment. Pretreatment with elf18 peptide decreased Agrobacterium-mediated transient expression efficiency more in wild-type seedlings than RTNLB4 O/E transgenic plants, which suggests that the induced defense responses in RTNLB4 O/E transgenic plants might be affected after bacterial elicitor treatments. Similarly, A. tumefaciens VirB2 peptide pretreatment reduced transient T-DNA expression in wild-type seedlings to a greater extent than in RTNLB4 O/E transgenic seedlings. Furthermore, the VirB2 peptides induced FRK1, WRKY22, and WRKY29 gene expression in wild-type seedlings but not efr-1 and bak1 mutants. The induced defense-related gene expression was lower in RTNLB4 O/E transgenic plants than wild-type seedlings after VirB2 peptide treatment. These data suggest that RTNLB4 may participate in elf18 and VirB2 peptide-induced defense responses and may therefore affect the A. tumefaciens infection process.

scholarly journals Early Responses of Tobacco Suspension Cells to Rhizobacterial Elicitors of Induced Systemic Resistance

2008 ◽  
Vol 21 (12) ◽  
pp. 1609-1621 ◽  
Author(s):  
Leendert C. van Loon ◽  
Peter A. H. M. Bakker ◽  
Walter H. W. van der Heijdt ◽  
David Wendehenne ◽  
Alain Pugin
Keyword(s):  
Plant Species ◽  
Cultured Cells ◽  
Induced Systemic Resistance ◽  
Active Oxygen Species ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Suspension Cells ◽  
Extracellular Medium ◽  
Tobacco Suspension Cells ◽  
Whole Plants

Colonization of roots by selected strains of fluorescent Pseudomonas spp. can trigger induced systemic resistance (ISR) against foliar pathogens in a plant species-specific manner. It has been suggested that early responses in cell suspension cultures in response to rhizobacterial elicitors, such as generation of active oxygen species (AOS) and extracellular medium alkalinization (MA), are linked to the development of ISR in whole plants. Perception of flagellin was demonstrated to elicit ISR in Arabidopsis, and bacterial lipopolysaccharides (LPS) have been shown to elicit several defense responses and to act as bacterial determinants of ISR in various plant species. In the present study, the LPS-containing cell walls, the pyoverdine siderophores, and the flagella of Pseudomonas putida WCS358, P. fluorescens WCS374, and P. fluorescens WCS417, which are all known to act as elicitors of ISR in selected plant species, were tested for their effects on the production of AOS, MA, elevation of cytoplasmic Ca2+ ([Ca2+]cyt), and defense-related gene expression in tobacco suspension cells. The LPS of all three strains, the siderophore of WCS374, and the flagella of WCS358 induced a single, transient, early burst of AOS, whereas the siderophores of WCS358 and WCS417 and the flagella of WCS374 and WCS417 did not. None of the compounds caused cell death. Once stimulated by the active compounds, the cells became refractory to further stimulation by any of the active elicitors, but not to the elicitor cryptogein from the oomycete Phytophthora cryptogea, indicating that signaling upon perception of the different rhizobacterial compounds rapidly converges into a common response pathway. Of all compounds tested, only the siderophores of WCS358 and WCS417 did not induce MA; the flagella of WCS374 and WCS417, although not active as elicitors of AOS, did induce MA. These results were corroborated by using preparations from relevant bacterial mutants. The active rhizobacterial elicitors led to a rapid increase in [Ca2+]cyt, peaking at 6 min, whereas the inactive siderophores of WCS358 and WCS417 elicited a single spike at 1 min. Elicitation of the cells by cell-wall LPS of WCS358 or the siderophore of WCS374 induced a weak, transient expression of several defense-related genes, including PAL and GST. The spectrum of early responses of the suspension cells was not matched by the expression of ISR in whole tobacco plants against Erwinia carotovora pv. carotovora. Of the live bacterial strains, only WCS358 elicited significant ISR, but application of the LPS or the siderophore of all three strains also elicited ISR. Notably, the absence of elicitation of AOS and MA in suspension-cultured cells but induction of ISR in whole plants by the siderophore of WCS358, which was lost upon treatment with the siderophore-minus mutant of WCS358, indicates that the early responses in suspension cells are not predictive of the ability to induce ISR in whole plants. Possible explanations for these discrepancies are discussed.

scholarly journals Arabidopsis thaliana Seedlings Influence Bacillus subtilis Spore Formation

2019 ◽  
Vol 32 (9) ◽  
pp. 1188-1195 ◽  
Author(s):  
Vincent Charron-Lamoureux ◽  
Pascale B. Beauregard
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacillus Subtilis ◽  
Systemic Resistance ◽  
Soil System ◽  
Beneficial Effects ◽  
Bacillus Subtilis Spore ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus subtilis is a Gram-positive plant-growth-promoting rhizobacterium exerting many beneficial effects on plant health. Because they secrete antimicrobial compounds and elicit induced systemic resistance, B. subtilis and phylogenetically related species are of particular interest as antifungals in organic agriculture. These bacteria are also known for their capacity to differentiate phenotypically into endospores able to withstand many environmental stresses. However, although B. subtilis is often inoculated on plants as spores, dynamics of germination and sporulation on roots remain unexplored. Using a hydroponic culture system and a soil system for Arabidopsis thaliana, we observed that B. subtilis spores germinate rapidly on contact with plants. However, the vegetative cells are abundant on roots for only a few days before reversing back to spores. We observed that the germinant receptor GerK and sporulation kinases KinA and KinB identified in vitro control sporulation dynamics on plants. Surprisingly, when plants are inoculated with B. subtilis, free-living cells sporulate more rapidly than plant-associated cells. However, direct contact between plant and bacteria is required for the induction of sporulation in the surrounding B. subtilis. This study has fundamental implications for our understanding of interactions between Bacillus spp. and plants, and particularly for a more efficient usage of B. subtilis as a biofertilizer or biofungicide.

PHÂN LẬP VÀ TUYỂN CHỌN CÁC CHỦNG BACILLUS ỨC CHẾ VI KHUẨN VIBRIO PARAHAEMOLYTICUS GÂY BỆNH TÔM CHẾT SỚM Ở TỈNH THỪA THIÊN HUẾ

2015 ◽  
Vol 100 (1) ◽  
Author(s):  
Nguyễn Thị Bích Đào ◽  
Trần Quang Khánh Vân ◽  
Nguyễn Văn Khanh ◽  
Nguyễn Quang Linh
Keyword(s):  
Bacillus Subtilis ◽  
Vibrio Parahaemolyticus ◽  
Bacillus Amyloliquefaciens ◽  
Bacillus Spp ◽  
Bacillus Subtilis B1

Khi tình hình bệnh hội chứng tôm chết sớm (EMS) đã gây thiệt hại vô cùng to lớn đối với Nuôi trồng thủy sản thì các giải pháp được đề nghị và áp dụng nhằm hạn chế dịch bệnh. Trong đó, việc tìm hiểu và đưa vi khuẩn có lợi để cạnh tranh và ức chế loài vi khuẩn gây bệnh rất được quan tâm, được cho là giải pháp có nhiều triển vọng phù hợp với điều kiện môi trường, đảm bảo sức khỏe cho con người, cũng như hạn chế được dịch bệnh. Đặc biệt, đưa vi khuẩn Bacillus spp. qua đường tiêu hóa của tôm ngay từ khi mới thả đã hạn chế được mật độ vi khuẩn Vibrio. Nghiên cứu này đã phân lập được các chủng Bacillus subtilis B1, Bacillus subtilis B2, Bacillus amyloliquefaciens B4và thử khả năng đối kháng với vi khuẩn Vibrio parahaemolyticus V1 ở các nồng độ 103, 104, 105, 106 CFU theo dõi ở các thời điểm 6h, 12h, 24h, 48h và 72h. Kết quả cho thấy cả ba chủng vi khuẩn Bacillus trên phân lập được đều có khả năng ức chế tốt vi khuẩn Vibrio parahaemolyticus V1, trong đó vi khuẩn Bacillus amyloliquefaciens B4 làtốt nhất với đường kính vòng kháng khuẩn 52,67 ± 4,31mm ở thời điểm 48h; hai chủng Bacillus subtilis B1, Bacillus subtilis B2 lầnlượt là  49,67 ± 3,15 mm, 44,07 ± 5,19 mm, với mức sai số có ý nghĩa thống kê p < 0,05.

scholarly journals An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mari Kurokawa ◽  
Masataka Nakano ◽  
Nobutaka Kitahata ◽  
Kazuyuki Kuchitsu ◽  
Toshiki Furuya
Keyword(s):  
Immune Responses ◽  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Large Scale ◽  
Plant Cells ◽  
Defense Responses ◽  
Root Tip ◽  
General Strategy ◽  
Plant Defense Responses ◽  
Microbe Interactions

AbstractMicroorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.

scholarly journals Induction of cell wall phenolic monomers as part of direct defense response in maize to pink stem borer (Sesamia inferens Walker) and non-insect interactions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
P. Lakshmi Soujanya ◽  
J. C. Sekhar ◽  
C. V. Ratnavathi ◽  
Chikkappa G. Karjagi ◽  
E. Shobha ◽  
...  
Keyword(s):  
Induced Defense ◽  
Stem Borer ◽  
Defense Responses ◽  
Short Term ◽  
Mechanical Wounding ◽  
Leaf Tissues ◽  
Short And Long Term ◽  
Insect Interactions ◽  
Induced Defense Responses

AbstractPink stem borer (PSB) causes considerable yield losses to maize. Plant–insect interactions have significant implications for sustainable pest management. The present study demonstrated that PSB feeding, mechanical wounding, a combination of mechanical wounding and PSB regurgitation and exogenous application of methyl jasmonate have induced phenolic compound mediated defense responses both at short term (within 2 days of treatment) and long term (in 15 days of treatment) in leaf and stalk tissues of maize. The quantification of two major defense related phenolic compounds namely p-Coumaric acid (p-CA) and ferulic acid (FA) was carried out through ultra-fast liquid chromatography (UFLC) at 2 and 15 days after imposing the above treatments. The p-CA content induced in leaf tissues of maize genotypes were intrinsically higher when challenged by PSB attack at V3 and V6 stages in short- and long-term responses. Higher p-CA content was observed in stalk tissues upon wounding and regurgitation in short- and long-term responses at V3 and V6 stages. Significant accumulation of FA content was also observed in leaf tissues in response to PSB feeding at V3 stage in long-term response while at V6 stage it was observed both in short- and long-term responses. In stalk tissues, methyl jasmonate induced higher FA content in short-term response at V3 stage. However, at V6 stage PSB feeding induced FA accumulation in the short-term while, wounding and regurgitation treatment-induced defense responses in the long-term. In general, the resistant (DMRE 63, CM 500) and moderately resistant genotypes (WNZ ExoticPool) accumulated significantly higher contents of p-CA and FA content than susceptible ones (CM 202, BML 6) in most of the cases. The study indicates that phenolic mediated defense responses in maize are induced by PSB attack followed by wounding and regurgitation compared to the other induced treatments. Furthermore, the study confirmed that induced defense responses vary with plant genotype, stage of crop growth, plant tissue and short and long-term responses. The results of the study suggested that the Phenolic acids i.e. p-CA and FA may contribute to maize resistance mechanisms in the maize-PSB interaction system.

scholarly journals Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance

2021 ◽  
Vol 9 (7) ◽  
pp. 1441
Author(s):  
Van Bach Lam ◽  
Thibault Meyer ◽  
Anthony Arguelles Arias ◽  
Marc Ongena ◽  
Feyisara Eyiwumi Oni ◽  
...  
Keyword(s):  
Rice Blast ◽  
Induced Systemic Resistance ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Systemic Resistance ◽  
Acid Sulfate Soils ◽  
Acid Sulfate ◽  
Wide Range ◽  
Bacillus Spp ◽  
Sulfate Soils

Rice monoculture in acid sulfate soils (ASSs) is affected by a wide range of abiotic and biotic constraints, including rice blast caused by Pyricularia oryzae. To progress towards a more sustainable agriculture, our research aimed to screen the biocontrol potential of indigenous Bacillus spp. against blast disease by triggering induced systemic resistance (ISR) via root application and direct antagonism. Strains belonging to the B. altitudinis and B. velezensis group could protect rice against blast disease by ISR. UPLC–MS and marker gene replacement methods were used to detect cyclic lipopeptide (CLiP) production and construct CLiPs deficient mutants of B. velezensis, respectively. Here we show that the CLiPs fengycin and iturin are both needed to elicit ISR against rice blast in potting soil and ASS conditions. The CLiPs surfactin, iturin and fengycin completely suppressed P. oryzae spore germination resulting in disease severity reduction when co-applied on rice leaves. In vitro microscopic assays revealed that iturin and fengycin inhibited the mycelial growth of the fungus P. oryzae, while surfactin had no effect. The capacity of indigenous Bacillus spp. to reduce rice blast by direct and indirect antagonism in ASS conditions provides an opportunity to explore their usage for rice blast control in the field.

scholarly journals Caterpillar salivary enzymes: "eliciting" a response

2004 ◽  
Vol 85 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Magali Merkx-Jacques ◽  
Jacqueline C. Bede
Keyword(s):  
Artificial Diet ◽  
Spodoptera Exigua ◽  
Induced Defense ◽  
Defense Responses ◽  
Beet Armyworm ◽  
Herbivorous Insect ◽  
Plant Responses ◽  
Plant Defense Responses ◽  
Induced Defense Responses ◽  
Bertha Armyworm

Abstract Plants exhibit remarkable plasticity in their ability to differentiate between herbivorous insect species and subtly adjust their defense responses to target distinct pests. One key mechanism used by plants to recognize herbivorous caterpillars is elicitors present in their oral secretions; however, these elicitors not only cause the induction of plant defenses but recent evidence suggests that they may also suppress plant responses. The absence of “expected changes” in induced defense responses of insect-infested plants has been attributed to hydrogen peroxide produced by caterpillar salivary glucose oxidase (GOX). Activity of this enzyme is variable among caterpillar species; it was detected in two generalist caterpillars, the beet armyworm (Spodoptera exigua) and the bertha armyworm (Mamestra configurata), but not in other generalist or specialist caterpillar species tested. In the beet armyworm, GOX activity fluctuated over larval development with high activity associated with the salivary glands of fourth instars. Larval salivary GOX activity of the beet armyworm and the bertha armyworm was observed to be significantly higher in caterpillars reared on artificial diet as compared with those reared on Medicago truncatula plants. This implies that a factor in the diet is involved in the regulation of caterpillar salivary enzyme activity. Therefore, plant diet may be regulating caterpillar oral elicitors that are involved in the regulation of plant defense responses: our goal is to understand these two processes.

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