scholarly journals A Dual-Color Imaging System for Sugarcane Smut Fungus Sporisorium scitamineum

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2357-2362 ◽  
Author(s):  
Meixin Yan ◽  
Enping Cai ◽  
Jianuan Zhou ◽  
Changqing Chang ◽  
Pinggen Xi ◽  
...  
Keyword(s):  
Life Cycle ◽  
Fluorescent Protein ◽  
Imaging System ◽  
Abiotic Factors ◽  
Smut Fungus ◽  
Color Imaging ◽  
Sporisorium Scitamineum ◽  
Dual Color ◽  
Life Cycle Development ◽  
Whole Life Cycle

The life cycle of the sugarcane smut fungus Sporisorium scitamineum is a multistep process. Haploid sporidia of compatible (MAT-1 versus MAT-2) mating types fuse to generate pathogenic dikaryotic hyphae to infect the host. Within the host tissues, diploid teliospores are formed and induce a characteristic sorus that looks like a black whip. The diploid teliospores germinate to form haploid sporidia by meiosis. In order to monitor fungal development throughout the whole life cycle, we expressed the green fluorescent protein (GFP) and red fluorescent protein (RFP) in S. scitamineum MAT-1 and MAT-2 sporidia, respectively. Observation by epifluorescence microscope showed that conjugation tube formation and sporidia fusion occurred at 4 to 8 h, and formation of dikaryotic filaments was detected at 12 h after mating. The resultant teliospores, with diffused GFP and RFP, underwent meiosis as demonstrated by septated hypha with single fluorescent signal. We demonstrated that GFP- and RFP-tagged strains can be used to study the life cycle development of the fungal pathogen S. scitamineum, including the sexual mating and meiosis events. This dual-color imaging system would be a valuable tool for investigation of biotic and abiotic factors that might affect the fungal life cycle development and pathogenesis.

scholarly journals Dual-Color Imaging of Nuclear Division and Mitotic Spindle Elongation in Live Cells of Aspergillus nidulans

2004 ◽  
Vol 3 (2) ◽  
pp. 553-556 ◽  
Author(s):  
Wenqi Su ◽  
Shihe Li ◽  
Berl R. Oakley ◽  
Xin Xiang
Keyword(s):  
Aspergillus Nidulans ◽  
Fluorescent Protein ◽  
Imaging System ◽  
Live Cells ◽  
Histone H2a ◽  
Nuclear Movement ◽  
Color Imaging ◽  
Alpha Tubulin ◽  
Dual Color ◽  
Spindle Elongation

ABSTRACT We have developed a dual-color imaging system based on cyan fluorescent protein-labeled histone H2A and green fluorescent protein-labeled alpha tubulin to visualize DNA and spindles simultaneously in the same living cell of Aspergillus nidulans. This system allows new details of mitosis and nuclear movement to be revealed.

Life Cycle Development of Successful Women-owned Enterprises

2020 ◽  
Vol 3 (4) ◽  
pp. 142-152
Author(s):  
Mohammad Waliul Hasanat ◽  
Kamna Anum ◽  
Ashikul Hoque ◽  
Mahmud Hamid ◽  
Sandy Francis Peris ◽  
...  
Keyword(s):  
Life Cycle ◽  
Data Collection ◽  
Research Work ◽  
Primary Data ◽  
Small Scale ◽  
Data Collection Process ◽  
Theoretical Finding ◽  
Successful Women ◽  
Two Phases ◽  
Life Cycle Development

In developing countries, the role of women in the business sector is continuously improving. As a result, female enterprises have also been encouraged in Pakistan. This study is based on life cycle development phases from which women-owned enterprises have to go through in order to become successful. As a primary data source, face-to-face interviews with owners of successful women-owned enterprises were preferred. The data collection process was divided into two phases i.e. Phase-I and Phase-II. After data collection, qualitative analysis has been performed using NVIVO. Findings provide both generic and specific factors involved in life cycle development of women-owned enterprises. This study provides a detailed view of life cycle development model followed by successful women enterprises. The outcome of this research work is a theoretical finding which can be utilized by entrepreneurs owning small scale enterprises to improve their level of performance. Findings can also be helpful for potentially talented women interested in setting up their own business.

scholarly journals Construction of exchange integrated information chain management model leading by information nurse for large instrument and equipment in operating room

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jinghua Dai ◽  
Xiaoqiang Ren ◽  
Peng Wu ◽  
Xiangdong Wang ◽  
Jiang Li ◽  
...  
Keyword(s):  
Life Cycle ◽  
Life Cycle Management ◽  
Management Model ◽  
Control Group ◽  
Equipment Management ◽  
Observation Group ◽  
Chain Management ◽  
Information Chain ◽  
Whole Life Cycle ◽  
Running Mode

Abstract Background This study aims to explore the information chain management model of large instrument and equipment inter-working in the operating room (OR) led by information nurses. Methods Through the chain management process of large instruments and equipment in the OR, which was based on information nurses, the management model of inter-working and integrating information chain was established, the key links were controlled, and the whole life cycle management of instruments and equipment from expected procurement to scrapping treatment was realized. Using the cluster sampling method, 1562 surgical patients were selected. Among these patients, 749 patients were assigned to the control group before the running mode, and 813 patients were assigned to the observation group after the running mode. The related indexes for large instrument and equipment management in the department before and after the running mode were compared. Results In the observation group, the average time of equipment registration was (22.05 ± 2.36), the cost was reduced by 2220 yuan/year, and the satisfaction rate of the nursing staff was 97.62%. These were significantly better, when compared to the control group (P < 0.05). Furthermore, the awareness rate of the whole staff for equipment repair application was 95.12%, and the arrival time of maintenance personnel and the examination and approval time of equipment management were greatly shortened (P < 0.05). Conclusion The integrated management model of large instrument and equipment interworking in the OR based on chain flow realizes the whole life cycle management of instruments and equipment, which is essential to improve management efficiency.

scholarly journals Determining the optimal expression method for dual-color imaging

2021 ◽  
Vol 351 ◽  
pp. 109064
Author(s):  
Jacob F. Norman ◽  
Bahar Rahsepar ◽  
Jad Noueihed ◽  
John A. White
Keyword(s):  
Color Imaging ◽  
Dual Color

scholarly journals BIM-Based Tools for Managing Construction and Demolition Waste (CDW): A Scoping Review

2021 ◽  
Vol 13 (15) ◽  
pp. 8427
Author(s):  
Bahareh Nikmehr ◽  
M. Reza Hosseini ◽  
Jun Wang ◽  
Nicholas Chileshe ◽  
Raufdeen Rameezdeen
Keyword(s):  
Life Cycle ◽  
Scoping Review ◽  
Construction And Demolition Waste ◽  
Future Research ◽  
Demolition Waste ◽  
Scant Attention ◽  
Intellectual Deficiencies ◽  
Whole Life Cycle ◽  
Point Of Reference ◽  
Design And Construction

This article provides a picture of the latest developments in providing BIM-based tools for construction and demolition waste (CDW) management. The coverage and breadth of the literature on offering BIM-based tools and technologies for dealing with CDW throughout the whole life cycle of construction are investigated, and gaps are identified. Findings reveal that, although various BIM-based technologies are closely associated with CDW, much of the existing research on this area has focused on the design and construction phase; indeed, the problem of CDW in post-construction stages has received scant attention. Besides, the now available tools and technologies are lacking in cross-phase insights into project waste aspects and are weak in theoretical rigor. This article contributes to the field by identifying the intellectual deficiencies in offering BIM-based tools and technologies when dealing with CDW. So, too, it points to major priorities for future research on the topic. For practitioners, the study provides a point of reference and raises awareness in the field about the most advanced available BIM-based technologies for dealing with CDW problems.

scholarly journals Flow cytometric measurements as a proxy for sporulation intensity in the cultured macroalga Ulva (Chlorophyta)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Omri Nahor ◽  
Cristina F. Morales-Reyes ◽  
Gianmaria Califano ◽  
Thomas Wichard ◽  
Alexander Golberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Life Cycle ◽  
Abiotic Factors ◽  
Physiological Status ◽  
Seaweed Cultivation ◽  
Reproductive Growth ◽  
Biotic And Abiotic Factors ◽  
Flow Cytometric ◽  
Abiotic Stimuli ◽  
Fundamental Shift

Abstract Controlling the life cycle of the green macroalga Ulva (Chlorophyta) is essential to maintain its efficient aquaculture. A fundamental shift in cultivation occurs by transforming the thallus cells into gametangia and sporangia (sporulation), with the subsequent release of gametes and zoids. Sporulation occurrence depends on algal age and abiotic stimuli and is controlled by sporulation inhibitors. Thus, quantification of sporulation intensity is critical for identifying the biotic and abiotic factors that influence the transition to reproductive growth. Here, we propose to determine the sporulation index by measuring the number of released gametes using flow cytometry, in proportion to the total number of thallus cells present before the occurrence of the sporulation event. The flow cytometric measurements were validated by manually counting the number of released gametes. We observed a variation in the autofluorescence levels of the gametes which were released from the gametangia. High autofluorescence level correlated to phototactically active behaviour of the gametes. As autofluorescence levels varied between different groups of gametes related to their mobility, flow cytometry can also determine the physiological status of the gametes used as feedstock in seaweed cultivation.

Innovation and Corporate Social Responsibility in SMEs

2015 ◽  
Vol 808 ◽  
pp. 359-363
Author(s):  
Cristina Feniser ◽  
Florin Lungu
Keyword(s):  
Corporate Social Responsibility ◽  
Life Cycle ◽  
Open Innovation ◽  
Social Goals ◽  
New Approach ◽  
Rational Management ◽  
Corporate Social ◽  
Qualitative Exploration ◽  
Whole Life Cycle ◽  
The Relationship

So far little attention has been given the differences or the compatibilities between CSR and innovation. Few works treats CSR in combination with innovation. What exactly is the relationship between CSR and innovation? Recent phenomena such as open innovation is based on the concept that the stakeholder's dialogue that overlaps with some dimensions of CSR. Being innovative means to bring organizational and technical improvements which will translate into a better position in the market. These improvements don't just aim the product, but the process by which it is obtained and its whole life-cycle. We're talking about a new approach to innovation, namely its orientation towards sustainability. Although SMEs have many features which facilitate implementation of CSR, activities of this type in such organizations are still limited. SMEs managers often make choices that are related to rational management. This leads to divergence between economical and social goals. Through a qualitative exploration of the concepts of CSR and innovation, we wanted to find out from some managers whether there is a link between innovation and CSR in SMEs and whether the activities corresponding to the two concepts overlap in a certain measure.

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