scholarly journals Molecular Typing of Bois Noir Phytoplasma Strains in the Chianti Classico Area (Tuscany, Central Italy) and Their Association with Symptom Severity in Vitis vinifera ‘Sangiovese’

2018 ◽  
Vol 108 (3) ◽  
pp. 362-373 ◽  
Author(s):  
R. Pierro ◽  
A. Passera ◽  
A. Panattoni ◽  
P. Casati ◽  
A. Luvisi ◽  
...  
Keyword(s):  
Symptom Severity ◽  
Central Italy ◽  
Mediterranean Area ◽  
Allelic Discrimination Assay ◽  
Allelic Discrimination ◽  
Bois Noir ◽  
Field Surveys ◽  
Eastern Pyrenees ◽  
Polyphagous Insect ◽  
First Time

Bois noir (BN) is the most widespread disease of the grapevine yellows complex in the Euro-Mediterranean area. BN is caused by ‘Candidatus Phytoplasma solani’ (BNp), transmitted from herbaceous plants to grapevine by polyphagous insect vectors. In this study, genetic diversity among BNp strains and their prevalence and possible association with grapevine symptom severity were investigated in a Sangiovese clone organic vineyard in the Chianti Classico area (Tuscany). Field surveys over 2 years revealed a range of symptom severity on grapevine and an increase of BN incidence. A TaqMan allelic discrimination assay detected only tufB type b among BNp strains, suggesting the prevalence of the bindweed-related ecology. Nucleotide sequence analyses of vmp1 and stamp genes identified 12 vmp1 and 16 stamp sequence variants, showing an overall positive selection for such genes. The prevalent genotype was Vm43/St10, reported for the first time in this study and closely related to strains identified only in the French Eastern Pyrenees. BNp strains identified in the examined vineyard and mostly grouped in separate bindweed-related phylogenetic clusters showed statistically significant differences in their distribution in grapevines exhibiting distinct symptom severity. These results suggest the possible occurrence of a range of virulence within BNp strain populations in the Chianti Classico area.

scholarly journals CD40Gene Polymorphisms Associated with Susceptibility and Coronary Artery Lesions of Kawasaki Disease in the Taiwanese Population

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Ho-Chang Kuo ◽  
Mei-Chyn Chao ◽  
Yu-Wen Hsu ◽  
Ying-Chi Lin ◽  
Ying-Hsien Huang ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Kawasaki Disease ◽  
Systemic Vasculitis ◽  
Recessive Model ◽  
Unknown Etiology ◽  
Nucleotide Polymorphisms ◽  
Taiwanese Population ◽  
Allelic Discrimination Assay ◽  
Allelic Discrimination ◽  
First Time

Background. Kawasaki disease (KD) is characterized by systemic vasculitis of unknown etiology. Our previous studies showed expression ofCD40ligand on CD4+ T cells correlated to the coronary artery lesion (CAL) and disease progress in KD. Other studies from Japan suggested the role ofCD40Lin the pathogenesis of CAL, and this might help explain the excessive number of males affected with KD but cannot be reproduced by Taiwanese population. This study was conducted to investigate theCD40polymorphism in KD and CAL formation.Methods. A total of 950 subjects (381 KD patients and 569 controls) were investigated to identify 2 tagging single-nucleotide polymorphisms (tSNPs) ofCD40(rs4810485 and rs1535045) by using the TaqMan allelic discrimination assay.Results. A significant association was noted with regards toCD40tSNPs (rs1535045) between controls and KD patients (P=0.0405, dominant model). In KD patients, polymorphisms ofCD40(rs4810485) showed significant association with CAL formation (P=0.0436, recessive model). Haplotype analysis did not yield more significant results between polymorphisms ofCD40and susceptibility/disease activity of KD.Conclusions. This study showed for the first time that polymorphisms ofCD40are associated with susceptibility to KD and CAL formation, in the Taiwanese population.

scholarly journals New challenges in the study of lithic raw materials in central Italy at the dawn of metal working societies: La Pietra and other radiolarite quarry-workshops in Tuscany

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Sem Scaramucci ◽  
Vanessa Volpi ◽  
Armando Costantini ◽  
Marco Giamello ◽  
Alessandro Donati ◽  
...  
Keyword(s):  
Raw Materials ◽  
Methodological Approach ◽  
Central Italy ◽  
Raw Material ◽  
Copper Age ◽  
Field Surveys ◽  
Archaeological Data ◽  
Geochemical Analyses ◽  
First Time ◽  
Non Destructive

In central-southern Tuscany radiolarite has been used as a lithic raw material throughout prehistory. During the Copper Age it was selected for the local production of leaf-shaped artefacts. In the area considered, the Copper Age record is almost totally restricted to burials and virtually no settlements have been investigated so far. Radiolarite artefacts are found mostly as refined arrow and, possibly javelin, heads used as grave goods.Within this context, the discovery and recent investigation of the large radiolarite quarry of La Pietra (Roccastrada, Grosseto) and of the related workshops is of great interest. Our aim here is to integrate the record from this site with other contemporary evidence of radiolarite exploitation. A programme of surveys has thus begun on the other radiolarite outcrops of the area in order to verify the existence of further rock quarrying or working. The discovery of a previously unknown quarry-workshop and two previously unknown workshops on radiolarite outcrops is presented here for the first time. The geological and archaeological data coming from the quarry-workshops will be used, in a future stage of research, to source the radiolarite artefacts found in Copper Age graves of Central Italy. The Copper age armatures are valuable artefacts mostly kept in museums and fully non-destructive analyses must be applied to them. To tackle these challenges, we followed a methodological approach which integrates field surveys, the individuation of petrographic markers of the most exploited radiolarite horizons and geochemical analyses. For geochemical characterization, we made use of pXRF portable spectrometer and here we present some preliminary results in the light of current methodological debate.In conclusion, even if some methodological questions remain open, we verified the feasibility of this programme of geographical, geological and geochemical characterizations and need now to increase our dataset in order to reconstruct a viable picture of Copper age lithic economy in central-southern Tuscany.

scholarly journals Identification and Characterization of New ‘Candidatus Phytoplasma solani’ Strains Associated with Bois Noir Disease in Vitis vinifera L. Cultivars Showing a Range of Symptom Severity in Georgia, the Caucasus Region

Plant Disease ◽  
2016 ◽  
Vol 100 (5) ◽  
pp. 904-915 ◽  
Author(s):  
Fabio Quaglino ◽  
David Maghradze ◽  
Paola Casati ◽  
Nona Chkhaidze ◽  
Mzagho Lobjanidze ◽  
...  
Keyword(s):  
Symptom Severity ◽  
Phylogenetic Analyses ◽  
Vitis Vinifera L ◽  
Polymorphism Analysis ◽  
South Caucasus ◽  
Bois Noir ◽  
Caucasus Region ◽  
Symptom Intensity ◽  
First Time

Evidence from a preliminary survey highlighted that ‘Candidatus Phytoplasma solani’, the etiological agent of bois noir (BN) disease of grapevine, infects grapevine varieties in Georgia, a country of the South Caucasus. In this study, field surveys were carried out to investigate the BN symptom severity in international and Georgian native varieties. ‘Ca. P. solani’ was detected and identified by polymerase chain reaction-based amplification and restriction fragment length polymorphism analysis of 16S ribosomal DNA, and further characterized by multiple gene typing analysis (vmp1 and stamp genes). Obtained data highlighted that the majority of Georgian grapevine varieties showed moderate and mild symptoms, whereas international cultivars exhibited severe symptoms. Molecular characterization of ‘Ca. P. solani’ from grapevine revealed the presence of 11 distinct phytoplasma types. Only one type (VmGe12/StGe7) was identical to a strain previously reported in periwinkle from Lebanon; the other ‘Ca. P. solani’ types are described here for the first time. Phylogenetic analyses of vmp1 and stamp gene concatenated nucleotide sequences showed that ‘Ca. P. solani’ strains in Georgia are associated mainly with the bindweed-related BN host system. Moreover, the fact that ‘Ca. P. solani’ strains are distributed in grapevine cultivars showing a range of symptom intensity suggests a different susceptibility of such local cultivars to BN.

scholarly journals Analysis of the influence of PTPN22 gene polymorphisms in systemic sclerosis

2010 ◽  
Vol 70 (3) ◽  
pp. 454-462 ◽  
Author(s):  
LM Diaz-Gallo ◽  
P Gourh ◽  
J Broen ◽  
C Simeon ◽  
V Fonollosa ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Meta Analysis ◽  
The Novel ◽  
Nucleotide Polymorphisms ◽  
Allelic Discrimination Assay ◽  
Allelic Discrimination ◽  
Single Nucleotide ◽  
Independent Replication ◽  
First Time

ObjectiveTwo functional single nucleotide polymorphisms (SNP) in the PTPN22 gene (rs24746601 and rs33996649) have been associated with autoimmunity. The aim of this study was to investigate the role of the R263Q SNP for the first time and to re-evaluate the role of the R620W SNP in the genetic predisposition to systemic sclerosis (SSc) susceptibility and clinical phenotypes.Methods3422 SSc patients (2020 with limited cutaneous SSc and 1208 with diffuse cutaneous SSc) and 3638 healthy controls of Caucasian ancestry from an initial case--control set of Spain and seven additional independent replication cohorts were included in our study. Both rs33996649 and rs2476601 PTPN22 polymorphisms were genotyped by TaqMan allelic discrimination assay. A meta-analysis was performed to test the overall effect of these PTPN22 polymorphisms in SSc.ResultsThe meta-analysis revealed evidence of association of the rs2476601 T allele with SSc susceptibility (pFDRcorrected=0.03 pooled, OR 1.15, 95% CI 1.03 to 1.28). In addition, the rs2476601 T allele was significantly associated with anticentromere-positive status (pFDRcorrected=0.02 pooled, OR 1.22, 95% CI 1.05 to 1.42). Although the rs33996649 A allele was significantly associated with SSc in the Spanish population (pFDRcorrected=0.04, OR 0.58, 95% CI 0.36 to 0.92), this association was not confirmed in the meta-analysis (p=0.36 pooled, OR 0.89, 95% CI 0.72 to 1.1).ConclusionThe study suggests that the PTPN22 R620W polymorphism influences SSc genetic susceptibility but the novel R263Q genetic variant does not. These data strengthen evidence that the R620W mutation is a common risk factor in autoimmune diseases.

scholarly journals Bioactive Compounds in Salicornia patula Duval-Jouve: A Mediterranean Edible Euhalophyte

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 410
Author(s):  
Irene Sánchez-Gavilán ◽  
Esteban Ramírez ◽  
Vicenta de la Fuente
Keyword(s):  
Fatty Acids ◽  
Bioactive Compounds ◽  
Mediterranean Area ◽  
Biological Properties ◽  
Total Phenolic ◽  
Tinto River ◽  
New Findings ◽  
High Concentrations ◽  
Salicornia Patula ◽  
First Time

Many halophytes have great nutritional and functional potential, providing chemical compounds with biological properties. Salicornia patula Duval-Jouve is a common euhalophyte from saline Mediterranean territories (Spain, Portugal, France, and Italy). In the present work we quantified for the first time the bioactive compounds in S. patula (total phenolic compounds and fatty acids), from Iberian Peninsula localities: littoral-coastal Tinto River basin areas (southwest Spain, the Huelva province), and mainland continental territories (northwest and central Spain, the Valladolid and Madrid provinces). Five phenolic acids including caffeic, coumaric, veratric, salicylic, and transcinnamic have been found with differences between mainland and coastal saltmarshes. S. patula contain four flavonoids: quercetin-3-O-rutinoside, kaempferol/luteolin, apigenin 7-glucoside, and pelargonidin-3-O-rutinoside. These last two glycosylated compounds are described for the first time in this genus of Chenopodiaceae. The fatty acid profile described in S. patula stems contains palmitic, oleic, and linoleic acids in high concentrations, while stearic and long-chain fatty acids were detected in low amounts. These new findings confirm that S. patula is a valuable source of bioactive compounds from Mediterranean area.

scholarly journals Environmental evolution, faunal and human occupation since 2 Ma in the Anagni basin, central Italy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fabio Florindo ◽  
Fabrizio Marra ◽  
Diego E. Angelucci ◽  
Italo Biddittu ◽  
Luciano Bruni ◽  
...  
Keyword(s):  
Central Italy ◽  
Time Frame ◽  
Archaeological Sites ◽  
Time Interval ◽  
Human Occupation ◽  
Environmental Evolution ◽  
Entire Interval ◽  
Field Surveys ◽  
Sedimentary Succession ◽  
Favorable Conditions

AbstractWe present the study of a composite, yet continuous sedimentary succession covering the time interval spanning 2.6–0.36 Ma in the intramontane basin of Anagni (central Italy) through a dedicated borecore, field surveys, and the review of previous data at the three palaeontological and archaeological sites of Colle Marino, Coste San Giacomo and Fontana Ranuccio. By combining the magneto- and chronostratigraphic data with sedimentologic and biostratigraphic analysis, we describe the palaeogeographic and tectonic evolution of this region during this entire interval. In this time frame, starting from 0.8 Ma, the progressive shallowing and temporary emersion of the large lacustrine basins and alluvial plains created favorable conditions for early hominin occupation of the area, as attested by abundant tool industry occurrences and fossils. This study provides new constraints to better interpret the hominin migratory dynamics and the factors that influenced the location and spatial distribution during the early occupation of this region.

scholarly journals An Italian Research Culture Collection of Wood Decay Fungi

Diversity ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 58 ◽  
Author(s):  
Carolina Elena Girometta ◽  
Annarosa Bernicchia ◽  
Rebecca Michela Baiguera ◽  
Francesco Bracco ◽  
Simone Buratti ◽  
...  
Keyword(s):  
Central Italy ◽  
Wood Decay ◽  
Mediterranean Area ◽  
Culture Collection ◽  
White Rot ◽  
Temperate Climate ◽  
Research Culture ◽  
Decay Fungi ◽  
Mn Peroxidase ◽  
The Mediterranean

One of the main aims of the University of Pavia mycology laboratory was to collect wood decay fungal (WDF) strains in order to deepen taxonomic studies, species distribution, officinal properties or to investigate potential applications such as biocomposite material production based on fungi. The Italian Alps, Apennines and wood plains were investigated to collect Basidiomycota basidiomata from living or dead trees. The purpose of this study was to investigate the wood decay strains of the Mediterranean area, selecting sampling sites in North and Central Italy, including forests near the Ligurian and Adriatic seas, or near the Lombardy lakes. The isolation of mycelia in pure culture was performed according to the current methodology and the identity of the strains was confirmed by molecular analyses. The strains are maintained in the Research Culture Collection MicUNIPV of Pavia University (Italy). Among the 500 WDF strains in the collection, the most interesting isolates from the Mediterranean area are: Dichomitus squalens (basidioma collected from Pinus pinea), Hericium erinaceus (medicinal mushroom), Inocutis tamaricis (white-rot agent on Tamarix trees), Perenniporia meridionalis (wood degrader through Mn peroxidase) and P. ochroleuca. In addition, strains of species related to the Mediterranean climate (e.g., Fomitiporia mediterranea and Cellulariella warnieri) were obtained from sites with a continental-temperate climate.

scholarly journals Assessment of the myostatin Q204X allele using an allelic discrimination assay

2006 ◽  
Vol 29 (3) ◽  
pp. 496-497
Author(s):  
Ana M. Sifuentes-Rincón ◽  
Herlinda E. Puentes-Montiel ◽  
Víctor R. Moreno-Medina ◽  
Xóchitl F. de la Rosa-Reyna
Keyword(s):  
Allelic Discrimination Assay ◽  
Allelic Discrimination

scholarly journals Parietaria Allergy: An Intriguing Challenge for the Allergist

Medicina ◽  
2018 ◽  
Vol 54 (6) ◽  
pp. 106
Author(s):  
Giorgio Ciprandi ◽  
Paola Puccinelli ◽  
Cristoforo Incorvaia ◽  
Simonetta Masieri
Keyword(s):  
Clinical Practice ◽  
Symptom Severity ◽  
Pollen Season ◽  
Clinical Characteristics ◽  
Allergen Immunotherapy ◽  
Correct Diagnosis ◽  
Mediterranean Area ◽  
Pollen Exposure ◽  
Inflammatory Changes ◽  
The Impact

Parietaria pollen is the most important cause of pollen allergies in the Mediterranean area, as Parietaria is widespread in this region. Many issues are associated with Parietaria allergy, including the duration of the pollen season (many doctors in fact believe that it lasts throughout the year), pollen load (which seems to be increasing over time), the impact of age (on IgE production and symptom severity), inflammatory changes (after pollen exposure), and the choice of allergen immunotherapy (AIT). In addition, molecular diagnostics allows for the defining of a correct diagnosis, differentiating between mere sensitization and true allergy. This review considers these topics and will hopefully help the allergist in clinical practice. Parietaria allergy is an intriguing challenge for the allergist in clinical practice, but it may be adequately managed by knowing the peculiarities of respective territories and the clinical characteristics of each patient.

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