scholarly journals Differential impacts of high fat and high Se diets on tissue lipid profiles of young adult and old wild‐type and GPX1‐/‐ mice (374.5)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Zeping Zhao ◽  
Jonggun Kim ◽  
Xin Gen Lei
Keyword(s):  
Young Adult ◽  
Lipid Profiles ◽  
Tissue Lipid ◽  
Wild Type ◽  
High Fat

High-caloric or isocaloric maternal high-fat diets differently affect young-adult offspring behavior in anxiety-related tests and offspring sensitivity to acute fluoxetine

2021 ◽  
Vol 403 ◽  
pp. 113141
Author(s):  
Carolina Cadete Lucena Cavalcanti ◽  
Raquel Da Silva Aragão ◽  
Erika Vanesa Cadena-Burbano ◽  
Thaynan Raquel dos Prazeres Oliveira ◽  
Jacqueline Maria Silva ◽  
...  
Keyword(s):  
Young Adult ◽  
Adult Offspring ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

scholarly journals Novel regulation of renal gluconeogenesis by Atp6ap2 in response to high fat diet via PGC1-α/AKT-1 pathway

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Safia Akhtar ◽  
Silas A. Culver ◽  
Helmy M. Siragy
Keyword(s):  
Protein Expression ◽  
High Fat Diet ◽  
Normal Diet ◽  
Receptor Expression ◽  
Wild Type ◽  
High Fat ◽  
Renal Gluconeogenesis ◽  
Mrna And Protein Expression ◽  
Polymerase Chain ◽  
Renal Expression

AbstractRecent studies suggested that renal gluconeogenesis is substantially stimulated in the kidney in presence of obesity. However, the mechanisms responsible for such stimulation are not well understood. Recently, our laboratory demonstrated that mice fed high fat diet (HFD) exhibited increase in renal Atp6ap2 [also known as (Pro)renin receptor] expression. We hypothesized that HFD upregulates renal gluconeogenesis via Atp6ap2-PGC-1α and AKT pathway. Using real-time polymerase chain reaction, western blot analysis and immunostaining, we evaluated renal expression of the Atp6ap2 and renal gluconeogenic enzymes, PEPCK and G6Pase, in wild type and inducible nephron specific Atp6ap2 knockout mice fed normal diet (ND, 12 kcal% fat) or a high-fat diet (HFD, 45 kcal% fat) for 8 weeks. Compared with ND, HFD mice had significantly higher body weight (23%) (P < 0.05), renal mRNA and protein expression of Atp6ap2 (39 and 35%), PEPCK (44 and 125%) and G6Pase (39 and 44%) respectively. In addition, compared to ND, HFD mice had increased renal protein expression of PGC-1α by 32% (P < 0.05) and downregulated AKT by 33% (P < 0.05) respectively in renal cortex. Atp6ap2-KO abrogated these changes in the mice fed HFD. In conclusion, we identified novel regulation of renal gluconeogenesis by Atp6ap2 in response to high fat diet via PGC1-α/AKT-1 pathway.

scholarly journals 12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response

2012 ◽  
Vol 302 (6) ◽  
pp. E654-E665 ◽  
Author(s):  
Banumathi K. Cole ◽  
Norine S. Kuhn ◽  
Shamina M. Green-Mitchell ◽  
Kendall A. Leone ◽  
Rebekah M. Raab ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Endoplasmic Reticulum ◽  
Stress Response ◽  
Er Stress ◽  
Pancreatic Islets ◽  
High Fat Diet ◽  
Wild Type ◽  
High Fat ◽  
Stress Markers ◽  
Deficient Mice

Central obesity is associated with chronic inflammation, insulin resistance, β-cell dysfunction, and endoplasmic reticulum (ER) stress. The 12/15-lipoxygenase enzyme (12/15-LO) promotes inflammation and insulin resistance in adipose and peripheral tissues. Given that obesity is associated with ER stress and 12/15-LO is expressed in adipose tissue, we determined whether 12/15-LO could mediate ER stress signals. Addition of 12/15-LO lipid products 12(S)-HETE and 12(S)-HPETE to differentiated 3T3-L1 adipocytes induced expression and activation of ER stress markers, including BiP, XBP-1, p-PERK, and p-IRE1α. The ER stress inducer, tunicamycin, upregulated ER stress markers in adipocytes with concomitant 12/15-LO activation. Addition of a 12/15-LO inhibitor, CDC, to tunicamycin-treated adipocytes attenuated the ER stress response. Furthermore, 12/15-LO-deficient adipocytes exhibited significantly decreased tunicamycin-induced ER stress. 12/15-LO action involves upregulation of interleukin-12 (IL-12) expression. Tunicamycin significantly upregulated IL-12p40 expression in adipocytes, and IL-12 addition increased ER stress gene expression; conversely, LSF, an IL-12 signaling inhibitor, and an IL-12p40-neutralizing antibody attenuated tunicamycin-induced ER stress. Isolated adipocytes and liver from 12/15-LO-deficient mice fed a high-fat diet revealed a decrease in spliced XBP-1 expression compared with wild-type C57BL/6 mice on a high-fat diet. Furthermore, pancreatic islets from 12/15-LO-deficient mice showed reduced high-fat diet-induced ER stress genes compared with wild-type mice. These data suggest that 12/15-LO activity participates in ER stress in adipocytes, pancreatic islets, and liver. Therefore, reduction of 12/15-LO activity or expression could provide a new therapeutic target to reduce ER stress and downstream inflammation linked to obesity.

scholarly journals CD40L controls obesity-associated vascular inflammation, oxidative stress, and endothelial dysfunction in high fat diet-treated and db/db mice

2017 ◽  
Vol 114 (2) ◽  
pp. 312-323 ◽  
Author(s):  
Sebastian Steven ◽  
Mobin Dib ◽  
Michael Hausding ◽  
Fatemeh Kashani ◽  
Matthias Oelze ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
High Fat Diet ◽  
Immune Cell ◽  
Vascular Inflammation ◽  
Human Study ◽  
Isometric Tension ◽  
Wild Type ◽  
High Fat ◽  
Aorta Ascendens

Abstract Aims CD40 ligand (CD40L) signaling controls vascular oxidative stress and related dysfunction in angiotensin-II-induced arterial hypertension by regulating vascular immune cell recruitment and platelet activation. Here we investigated the role of CD40L in experimental hyperlipidemia. Methods and results Male wild type and CD40L−/− mice (C57BL/6 background) were subjected to high fat diet for sixteen weeks. Weight, cholesterol, HDL, and LDL levels, endothelial function (isometric tension recording), oxidative stress (NADPH oxidase expression, dihydroethidium fluorescence) and inflammatory parameters (inducible nitric oxide synthase, interleukin-6 expression) were assessed. CD40L expression, weight, leptin and lipids were increased, and endothelial dysfunction, oxidative stress and inflammation were more pronounced in wild type mice on a high fat diet, all of which was almost normalized by CD40L deficiency. Similar results were obtained in diabetic db/db mice with CD40/TRAF6 inhibitor (6877002) therapy. In a small human study higher serum sCD40L levels and an inflammatory phenotype were detected in the blood and Aorta ascendens of obese patients (body mass index > 35) that underwent by-pass surgery. Conclusion CD40L controls obesity-associated vascular inflammation, oxidative stress and endothelial dysfunction in mice and potentially humans. Thus, CD40L represents a therapeutic target in lipid metabolic disorders which is a leading cause in cardiovascular disease.

scholarly journals Ameliorative Effects of Aloe Vera Gel Extract on Circulatory and Tissue Lipid Profile Status in Streptozotocin-Induced Diabetic Rats

2018 ◽  
Vol 9 (1) ◽  
pp. 11-26
Author(s):  
RN Ugbaja ◽  
DI Akinloye ◽  
AJ Akamo ◽  
EI Ugwor ◽  
OO IIesanmi ◽  
...  
Keyword(s):  
Aloe Vera ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipid Profiles ◽  
Albino Rats ◽  
Tissue Lipid ◽  
Rbc Membrane ◽  
Aloe Vera Gel ◽  
Lipid Parameters ◽  
Scientific Rationale

Diabetes is characterized by hyperglycaemia with alterations of lipid parameters. The effects of Aloe vera (A.V) gel extract on lipid profiles of streptozotocin-induced diabetic rats were investigated. Seventy-two male albino rats were randomly divided into six groups of 12 rats each; control, diabetic control, 4 ml/kg and 8 ml/kg body weight (b.w.) A.V, 10 mg/kg b.w. glibenclimide and non-diabetic 8 ml/kg b.w. A.V groups. They were treated for 14 and 28 days. The plasma and HDL lipid profiles (triacylglycerols, cholesterol and phospholipids) of the diabetic rats were significantly (p<0.05) increased, while those of the VLDL+LDL, RBC, RBC membrane and organs were observed to be significantly (p<0.05) reduced, when compared to the control after 14 and 28 days of treatment. A.V gel extract significantly (p<0.05) ameliorated the disruptions observed in the lipid profiles of the diabetic and thus provide a scientific rationale for the use of Aloe vera as a therapeutic agent.Bangladesh J Med Biochem 2016; 9(1): 11-26

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