scholarly journals A simple, high-resolution, non-destructive method for determining the spatial gradient of the elastic modulus of insect cuticle

2018 ◽  
Vol 15 (145) ◽  
pp. 20180312 ◽  
Author(s):  
S h. Eshghi ◽  
M. Jafarpour ◽  
A. Darvizeh ◽  
S. N. Gorb ◽  
H. Rajabi
Keyword(s):  
Elastic Modulus ◽  
High Resolution ◽  
Three Dimensional ◽  
Insect Cuticle ◽  
Spatial Gradient ◽  
Engineering Systems ◽  
Small Complex ◽  
Load Carrying ◽  
Using Data ◽  
Non Destructive

Nature has evolved structures with high load-carrying capacity and long-term durability. The principles underlying the functionality of such structures, if studied systematically, can inspire the design of more efficient engineering systems. An important step in this process is to characterize the material properties of the structure under investigation. However, direct mechanical measurements on small complex-shaped biological samples involve numerous technical challenges. To overcome these challenges, we developed a method for estimation of the elastic modulus of insect cuticle, the second most abundant biological composite in nature, through simple light microscopy. In brief, we established a quantitative link between the autofluorescence of different constituent materials of insect cuticle, and the resulting mechanical properties. This approach was verified using data on cuticular structures of three different insect species. The method presented in this study allows three-dimensional visualisation of the elastic modulus, which is impossible with any other available technique. This is especially important for precise finite-element modelling of cuticle, which is known to have spatially graded properties. Considering the simplicity, ease of implementation and high-resolution of the results, our method is a crucial step towards a better understanding of material–function relationships in insect cuticle, and can potentially be adapted for other graded biological materials.

High-resolution non-destructive three-dimensional imaging of integrated circuits

Nature ◽  
2017 ◽  
Vol 543 (7645) ◽  
pp. 402-406 ◽  
Author(s):  
Mirko Holler ◽  
Manuel Guizar-Sicairos ◽  
Esther H. R. Tsai ◽  
Roberto Dinapoli ◽  
Elisabeth Müller ◽  
...  
Keyword(s):  
High Resolution ◽  
Integrated Circuits ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Non Destructive

Photo-Acoustic Based Non-Contact and Non-Destructive Evaluation for Detection of Damage Precursors in Composites

2018 ◽  
Author(s):  
Siqi Wang ◽  
Liangzhong Xiang ◽  
Yingtao Liu ◽  
Hong Liu
Keyword(s):  
High Resolution ◽  
Imaging System ◽  
Three Dimensional ◽  
Pulsed Laser ◽  
Complex Load ◽  
Pull Out ◽  
Matrix Cracks ◽  
Non Destructive Evaluation ◽  
Cfrp Composites ◽  
Non Destructive

Damage precursor in composites can lead to large structural damages, such as delamination, in carbon fiber reinforced plastic (CFRP) composites due to complex load conditions and environmental effects. In addition, multiple types of damage precursors including micro-scale matrix cracks, fiber pull-out from matrix, and fiber breakages, are extremely difficult to detect due to the limitation of resolution of current non-destructive evaluation (NDE) technologies. This paper presents a photo-acoustic based non-contact NDE system for the detection of damage precursors with extremely high resolution up to one hundred micrometers. This system consists of three major components: picoseconds pulsed laser based ultrasonic actuator, ultrasound receiver, and data processing and computing subsystem. Picoseconds pulsed laser is used to generate ultrasonic propagations in composites during the NDE process, and the ultrasound signals are recorded by the ultrasound receiver. Three-dimensional microstructure of the individual composites grid within the composite is able to be reconstructed for further analysis. The size and position of the damage precursors are evaluated with high accuracy up to 100 μm. The experimental results demonstrate that this imaging system is able to provide a novel non-contact approach with extremely high resolution for damage detection of CFRP composites. In addition, the developed NDE system has a wide industrial application in aerospace, automobile, civil, mechanical, and other key industries.

Spatiotemporal tomography based on scattered multiangular signals and its use for resolving evolving clouds using moving platforms

2021 ◽  
Author(s):  
Roi Ronen ◽  
Yoav Y. Schechner ◽  
Eshkol Eytan
Keyword(s):  
High Resolution ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Remote Sensing Data ◽  
Real Data ◽  
Moving Platforms ◽  
Temporal Sampling ◽  
Heterogeneous Objects ◽  
Large Numbers ◽  
Using Data

<p>The climate is strongly affected by interaction with clouds. To reduce major errors in climate predictions, this interaction requires a much finer understanding of cloud physics than current knowledge. Current knowledge is based on empirical remote sensing data that is analyzed under the assumption that the atmosphere and clouds are made of very broad and uniform layers. To help to overcome this problem, 3D scattering computed tomography (CT) has been suggested as a way to study clouds. </p><p>CT is a powerful way to recover the inner structure of three dimensional (3D) volumetric heterogeneous objects. CT has extensive use in many research and operational domains. Aside from its common usage in medicine, CT is used for sensing geophysical terrestrial structures, atmospheric pollution and fluid dynamics. CT requires imaging from multiple directions and in nearly all CT approaches, the object is considered static during image acquisition. However, in many cases, the object changes while multi-view images are acquired sequentially. Thus, an effort has been invested to expand 3D CT to four-dimensional (4D) spatiotemporal CT. This effort has been directed at linear CT modalities. Since linear CT is computationally easier to handle, it has been a popular method for medical imaging. However, these linear CT modalities do not apply to clouds: clouds constitute a scattering medium, and therefore radiative transfer is non-linear in the clouds’ content.</p><p>This work focuses on the challenge of 4D scattering CT of clouds. Scattering CT of clouds requires high-resolution multi-view images from space. There are spaceborne and high-altitude systems that may provide such data, for example AirMSPI, MAIA, HARP and AirHARP. An additional planned system is the CloudCT formation, funded by the ERC. However, these systems are costly. Deploying them in large numbers to simultaneously acquire images of the same clouds from many angles can be impractical. Therefore, the platforms are planned to move above the clouds: a sequence of images is taken, in order to span and sample a wide angular breadth. However, the clouds evolve while the angular span is sampled.</p><p>We pose conditions under which this task can be performed. These regard temporal sampling and angular breadth, in relation to the correlation time of the evolving cloud. Then, we generalize scattering CT. The generalization seeks spatiotemporal recovery of the cloud extinction field in high resolution (10m), using data taken by a small number of moving cameras. We present an optimization-based method to reach this, and then demonstrate the method both in rigorous simulations and on real data.</p>

scholarly journals MicroCT as a Useful Tool for Analysing the 3D Structure of Lichens and Quantifying Internal Cephalodia in Lobaria pulmonaria

2021 ◽  
Vol 1 (2) ◽  
pp. 189-200
Author(s):  
Julia Gerasimova ◽  
Bernhard Ruthensteiner ◽  
Andreas Beck
Keyword(s):  
High Resolution ◽  
Computer Tomography ◽  
Structural Characteristics ◽  
3D Structure ◽  
Three Dimensional ◽  
Structural Features ◽  
Lobaria Pulmonaria ◽  
X Ray ◽  
Yield Information ◽  
Non Destructive

High-resolution X-ray computer tomography (microCT) is a well-established technique to analyse three-dimensional microstructures in 3D non-destructive imaging. The non-destructive three-dimensional analysis of lichens is interesting for many reasons. The examination of hidden structural characteristics can, e.g., provide information on internal structural features (form and distribution of fungal-supporting tissue/hypha), gas-filled spaces within the thallus (important for gas exchange and, thus, physiological processes), or yield information on the symbiont composition within the lichen, e.g., the localisation and amount of additional cyanobacteria in cephalodia. Here, we present the possibilities and current limitations for applying conventional laboratory-based high-resolution X-ray computer tomography to analyse lichens. MicroCT allows the virtual 3D reconstruction of a sample from 2D X-ray projections and is helpful for the non-destructive analysis of structural characters or the symbiont composition of lichens. By means of a quantitative 3D image analysis, the volume of internal cephalodia is determined for Lobaria pulmonaria and the external cephalodia of Peltigera leucophlebia. Nevertheless, the need for higher-resolution tomography for more detailed studies is emphasised. Particular challenges are the large sizes of datasets to be analysed and the high variability of the lichen microstructures.

High Resolution Microscopy of Multi-Layered Biological Crystals

1982 ◽  
Vol 40 ◽  
pp. 80-83
Author(s):  
H.A. Cohen ◽  
T.W. Jeng ◽  
W. Chiu
Keyword(s):  
High Resolution ◽  
Low Dose ◽  
Three Dimensional ◽  
Data Interpretation ◽  
Two Dimensional ◽  
Biological Objects ◽  
Density Maps ◽  
Density Map ◽  
Complex Crystal ◽  
High Resolution Microscopy

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.

Electron crystallographic determination of the 3-D structure of staurolite at atomic resolution

1991 ◽  
Vol 49 ◽  
pp. 540-541
Author(s):  
Kenneth H. Downing ◽  
Hu Meisheng ◽  
Hans-Rudolf Went ◽  
Michael A. O'Keefe
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Electron Microscopy ◽  
Atomic Resolution ◽  
Dimensional Structure ◽  
Structure Information ◽  
Resolution Electron ◽  
Scattering Effects ◽  
High Resolution Images ◽  
Effects Of Radiation

With current advances in electron microscope design, high resolution electron microscopy has become routine, and point resolutions of better than 2Å have been obtained in images of many inorganic crystals. Although this resolution is sufficient to resolve interatomic spacings, interpretation generally requires comparison of experimental images with calculations. Since the images are two-dimensional representations of projections of the full three-dimensional structure, information is invariably lost in the overlapping images of atoms at various heights. The technique of electron crystallography, in which information from several views of a crystal is combined, has been developed to obtain three-dimensional information on proteins. The resolution in images of proteins is severely limited by effects of radiation damage. In principle, atomic-resolution, 3D reconstructions should be obtainable from specimens that are resistant to damage. The most serious problem would appear to be in obtaining high-resolution images from areas that are thin enough that dynamical scattering effects can be ignored.

Three-Dimensional Immunoelectron Microscopy of Yeast Cells by a New High-Resolution Replica Method

1985 ◽  
Vol 43 ◽  
pp. 562-563
Author(s):  
Hirano T. ◽  
M. Yamaguchi ◽  
M. Hayashi ◽  
Y. Sekiguchi ◽  
A. Tanaka
Keyword(s):  
High Resolution ◽  
Plasma Polymerization ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Replica Method ◽  
Yeast Cells ◽  
Dynamic Aspect ◽  
Replica Technique ◽  
Gaseous Hydrocarbon ◽  
Transmission Electron

A plasma polymerization film replica method is a new high resolution replica technique devised by Tanaka et al. in 1978. It has been developed for investigation of the three dimensional ultrastructure in biological or nonbiological specimens with the transmission electron microscope. This method is based on direct observation of the single-stage replica film, which was obtained by directly coating on the specimen surface. A plasma polymerization film was deposited by gaseous hydrocarbon monomer in a glow discharge.The present study further developed the freeze fracture method by means of a plasma polymerization film produces a three dimensional replica of chemically untreated cells and provides a clear evidence of fine structure of the yeast plasma membrane, especially the dynamic aspect of the structure of invagination (Figure 1).

Pavement Damage Due to Conventional and New Generation of Wide-Base Super Single Tires

2005 ◽  
Vol 33 (4) ◽  
pp. 210-226 ◽  
Author(s):  
I. L. Al-Qadi ◽  
M. A. Elseifi ◽  
P. J. Yoo ◽  
I. Janajreh
Keyword(s):  
Carrying Capacity ◽  
Material Properties ◽  
Three Dimensional ◽  
Fe Analysis ◽  
Load Carrying Capacity ◽  
Inflation Pressure ◽  
3D Finite Element ◽  
Load Carrying ◽  
Pavement Damage ◽  
New Generation

Abstract The objective of this study was to quantify pavement damage due to a conventional (385/65R22.5) and a new generation of wide-base (445/50R22.5) tires using three-dimensional (3D) finite element (FE) analysis. The investigated new generation of wide-base tires has wider treads and greater load-carrying capacity than the conventional wide-base tire. In addition, the contact patch is less sensitive to loading and is especially designed to operate at 690kPa inflation pressure at 121km/hr speed for full load of 151kN tandem axle. The developed FE models simulated the tread sizes and applicable contact pressure for each tread and utilized laboratory-measured pavement material properties. In addition, the models were calibrated and properly validated using field-measured stresses and strains. Comparison was established between the two wide-base tire types and the dual-tire assembly. Results indicated that the 445/50R22.5 wide-base tire would cause more fatigue damage, approximately the same rutting damage and less surface-initiated top-down cracking than the conventional dual-tire assembly. On the other hand, the conventional 385/65R22.5 wide-base tire, which was introduced more than two decades ago, caused the most damage.

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