The equilibrium and stability of sessile drops

The paper gives a mathematical analysis of the equilibrium and stability of two dimensional and axisymmetric sessile drops, when controlled by their volume or by their internal pressure. Sessile drops of either form when standing on a horizontal plane with a prescribed contact angle are shown to be stable when controlled by their volume or pressure, except for the neutrally stable sideways translation, without change of shape, which can occur formally. For two dimensional drops grown on a fixed base, and controlled by their volume, plane symmetric, plane asymmetric, and three dimensional forms of instability can occur, the last named being the first one to occur, at the position of the internal pressure maximum. For the same drop when controlled by its pressure plane symmetric and three dimensional instabilities arise together at the pressure maximum. For axisymmetric drops grown on a fixed base and controlled by their volume, axisymmetric and asymmetric instabilities can arise. Asymmetric instability with azimuthal wavenumber m = 1 arises when the drop profile becomes horizontal at its base, and this instability always precedes the axisymmetric instability arising at the volume maximum. However, the same drop when controlled by its pressure becomes unstable first in the axisymmetric mode arising at the pressure maximum.

The paper gives a mathematical account of the equilibrium and the stability of equilibrium for two dimensional pendent drops hanging under the action of gravity and surface tension forces. The drops may be formed under conditions of constant volume, as when they hang from the horizontal lower edge of a vertical plate. Alternatively, equilibrium may be maintained under conditions of constant pressure, as, for example, when a drop is formed in a narrow gap between two plane parallel vertical plates when the fluid between the plates is connected to a reservoir of large free surface area. Equilibrium profiles, symmetric about a vertical centre plane, are calcu­lated for drops hanging from horizontal apertures of varying widths. It is shown that the volume of the suspended drop achieves a maximum as a function of, say, its depth, for each given aperture. For apertures of width less than a critical value π in capillary units of length, it is found that the pressure, as measured by the internal pressure at the aperture in excess of the external pressure, also achieves a positive maximum. If the volume and depth of the drop are gradually raised from zero the pressure maximum occurs before the volume maximum is reached. For an aperture of width π the pressure maximum is reduced to zero and the maximum point occurs at the initial position where the volume and depth are zero. For apertures greater than π the pressure decreases monotonically from zero as the depth is raised from zero. In the discussion of the stability we distinguish between two and three dimensional disturbances, and between disturbances which are symmetric or asymmetric about the centre plane of symmetry of the equilibrium profile. Drops maintained at constant volume are shown to have a limit point instability to two dimensional weak disturbances symmetric about the centre plane, which occurs at the point of maximum volume. Likewise, drops maintained at constant pressure in an aperture of width less than π, have a limit point instability to disturbances of the same form, which occurs at the point of maximum pressure. The analysis shows that for apertures greater than π drops cannot be formed under conditions of constant pressure, since they are unstable from the initial position of zero depth and volume. A brief discussion is given for the instability to plane asymmetric disturbances, which we find to be unimportant in the sense that these instabilities cannot occur before the equilibrium has already become unstable to disturbances of symmetric type. The most important source of instability of the two dimensional drop is the three dimensional disturbance, that is, one having a sinuous variation along the length of the drop. The stability to such displacements is studied in the last main section of the paper. It is shown there that instability of this form, with disturbances symmetric about the vertical centre plane, will begin to arise for apertures less than π when the equilibrium profile reaches the pressure maximum, for drops maintained both at constant volume and at constant pressure. For drops maintained at constant pressure this does not make any change in the position of the first onset of instability, since, then, two and three dimensional instabilities arise at the same point. However, for drops maintained at constant volume there is a marked change in behaviour. Since the pressure maximum occurs at a lower volume than the volume maximum, and at a lower depth, the drop maintained at constant volume will always become unstable to three dimensional instability first. The nature of the equilibrium for apertures greater than π is such that the three dimensional instability sets in at the outset, in this case for drops of both kinds. This is in agreement with a result of Plateau (1873) and Maxwell (1875), who studied the stability of a plane horizontal interface in a slit, and showed that for a slit of width greater than π the plane interface is not stable to small displacements which conserve the volume. Finally, we have considered three dimensional disturbances which are asymmetric about the centre plane, and we have shown that instabilities of this form cannot arise.


Author(s):  
B. Yoo ◽  
R. F. Kulak

This paper presents findings from our initial work in developing a seismic isolation system for the STAR-LM reactor design. Research and development was carried out to determine the characteristics of the isolator device. The heavy weight and small footprint presented a challenge in bearing design and bearing placement. Results are also presented from a study on the use of three-dimensional seismic isolation devices to the full-scale reactor. Both two-dimensional (i.e., one device for horizontal isolation only) and integral (i.e., one device for horizontal and vertical) concepts were explored. The seismic analysis responses of the two-dimensional and the three-dimensional isolation systems for the STAR-LM are compared with that of the conventional fixed base system. Finally, results are presented from a study on the effects of the levels of vertical and horizontal damping on the seismic response of STAR-LM.


Author(s):  
H.A. Cohen ◽  
T.W. Jeng ◽  
W. Chiu

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.


Author(s):  
B. Ralph ◽  
A.R. Jones

In all fields of microscopy there is an increasing interest in the quantification of microstructure. This interest may stem from a desire to establish quality control parameters or may have a more fundamental requirement involving the derivation of parameters which partially or completely define the three dimensional nature of the microstructure. This latter categorey of study may arise from an interest in the evolution of microstructure or from a desire to generate detailed property/microstructure relationships. In the more fundamental studies some convolution of two-dimensional data into the third dimension (stereological analysis) will be necessary.In some cases the two-dimensional data may be acquired relatively easily without recourse to automatic data collection and further, it may prove possible to perform the data reduction and analysis relatively easily. In such cases the only recourse to machines may well be in establishing the statistical confidence of the resultant data. Such relatively straightforward studies tend to result from acquiring data on the whole assemblage of features making up the microstructure. In this field data mode, when parameters such as phase volume fraction, mean size etc. are sought, the main case for resorting to automation is in order to perform repetitive analyses since each analysis is relatively easily performed.


Author(s):  
Yu Liu

The image obtained in a transmission electron microscope is the two-dimensional projection of a three-dimensional (3D) object. The 3D reconstruction of the object can be calculated from a series of projections by back-projection, but this algorithm assumes that the image is linearly related to a line integral of the object function. However, there are two kinds of contrast in electron microscopy, scattering and phase contrast, of which only the latter is linear with the optical density (OD) in the micrograph. Therefore the OD can be used as a measure of the projection only for thin specimens where phase contrast dominates the image. For thick specimens, where scattering contrast predominates, an exponential absorption law holds, and a logarithm of OD must be used. However, for large thicknesses, the simple exponential law might break down due to multiple and inelastic scattering.


Author(s):  
D. E. Johnson

Increased specimen penetration; the principle advantage of high voltage microscopy, is accompanied by an increased need to utilize information on three dimensional specimen structure available in the form of two dimensional projections (i.e. micrographs). We are engaged in a program to develop methods which allow the maximum use of information contained in a through tilt series of micrographs to determine three dimensional speciman structure.In general, we are dealing with structures lacking in symmetry and with projections available from only a limited span of angles (±60°). For these reasons, we must make maximum use of any prior information available about the specimen. To do this in the most efficient manner, we have concentrated on iterative, real space methods rather than Fourier methods of reconstruction. The particular iterative algorithm we have developed is given in detail in ref. 3. A block diagram of the complete reconstruction system is shown in fig. 1.


Author(s):  
A.M. Jones ◽  
A. Max Fiskin

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.


Author(s):  
Douglas L. Dorset ◽  
Andrew K. Massalski

Matrix porin, the ompF gene product of E. coli, has been the object of a electron crystallographic study of its pore geometry in an attempt to understand its function as a membrane molecular sieve. Three polymorphic forms have been found for two-dimensional crystals reconstituted in phospholipid, two hexagonal forms with different lipid content and an orthorhombic form coexisting with and similar to the hexagonal form found after lipid loss. In projection these have been shown to retain the same three-fold pore triplet geometry and analyses of three-dimensional data reveal that the small hexagonal and orthorhombic polymorphs have similar structure as well as unit cell spacings.


Author(s):  
Jeffry A. Reidler ◽  
John P. Robinson

We have prepared two-dimensional (2D) crystals of tetanus toxin using procedures developed by Uzgiris and Kornberg for the directed production of 2D crystals of monoclonal antibodies at an antigen-phospholipid monolayer interface. The tetanus toxin crystals were formed using a small mole fraction of the natural receptor, GT1, incorporated into phosphatidyl choline monolayers. The crystals formed at low concentration overnight. Two dimensional crystals of this type are particularly useful for structure determination using electron microscopy and computer image refinement. Three dimensional (3D) structural information can be derived from these crystals by computer reconstruction of photographs of toxin crystals taken at different tilt angles. Such 3D reconstructions may help elucidate the mechanism of entry of the enzymatic subunit of toxins into cells, particularly since these crystals form directly on a membrane interface at similar concentrations of ganglioside GT1 to the natural cellular receptors.


Author(s):  
José L. Carrascosa ◽  
José M. Valpuesta ◽  
Hisao Fujisawa

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.


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