scholarly journals Decreased Erythrocyte NA+,K+-ATPase Activity and Increased Plasma TBARS in Prehypertensive Patients

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Carlos Ricardo Maneck Malfatti ◽  
Leandro Tibiriçá Burgos ◽  
Alexandre Rieger ◽  
Cássio Luiz Rüdger ◽  
Janaína Angela Túrmina ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Atpase Activity ◽  
Cell Damage ◽  
Venous Blood ◽  
Thiobarbituric Acid ◽  
Normal Blood Pressure ◽  
Thiobarbituric Acid Reactive Substances ◽  
Normotensive Subjects ◽  
The Relationship ◽  
Membrane Cell

The essential hypertension has been associated with membrane cell damage. The aim of the present study is investigate the relationship between erythrocyte Na+,K+-ATPase and lipoperoxidation in prehypertensive patients compared to normotensive status. The present study involved the prehypertensive patients (systolic:136±7 mmHg; diastolic:86.8±6.3 mmHg;n=8) and healthy men with normal blood pressure (systolic:110±6.4 mmHg; diastolic:76.1±4.2 mmHg;n=8) who were matched for age (35±4years old). The venous blood samples of antecubital vein (5 mL) were collected into a tube containing sodium heparin as anticoagulant (1000 UI), and erythrocyte ghosts were prepared for quantifying Na+,K+-ATPase activity. The extent of the thiobarbituric acid reactive substances (TBARS) was determined in plasma. The statistical analysis was carried out by Student’st-test and Pearson’s correlation coefficient. AP<0.05was considered significant. The Na+,K+-ATPase activity was lower in prehypertensive patients compared with normotensive subjects (4.9 versus 8.0 nmol Pi/mg protein/min;P<0.05). The Na+,K+-ATPase activity correlated negatively with TBARS content (r=-0.6;P<0.05) and diastolic blood pressure (r=-0.84;P<0.05). The present study suggests that Na+,K+-ATPase activity reduction and elevation of the TBARS content may underlie the pathophysiological aspects linked to the prehypertensive status.

Effect of Superchilling Storage on Quality Characterizes and Physical Properties of Surimi from Silver Carp (Hypophthalmichthys molitrix) as Compared with Cooling Freshness Preservation

2012 ◽  
Vol 554-556 ◽  
pp. 978-984
Author(s):  
Qian Liu ◽  
Jian Chun Han ◽  
Yong Gen Zhang ◽  
Shuang Mei Li ◽  
Jing Li ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Physical Properties ◽  
Storage Time ◽  
Ph Value ◽  
Silver Carp ◽  
Thiobarbituric Acid ◽  
Raw Material ◽  
Hypophthalmichthys Molitrix ◽  
Thiobarbituric Acid Reactive Substances ◽  
Fresh Products

Quality characterizes of surimi of silver carp at two different conditions of superchilling and cooling freshness preservation. The pH value, thiobarbituric acid-reactive substances (TBARS value), protein soubility and ATPase activity were studied. The result showed that pH value and TBARS value increased with the increasing of the storage time (P < 0.05), protein soubility and both ATPase activity decreased with the increasing of the storage time (P < 0.05). In general, superchilling is a good way to preserve freshness of fresh products and the raw material before processing, and also could have great effect on improving the quality characterizes of surimi and prolong its shelf life.

Intralymphocytic Sodium in Hypertensive Patients: A Significant Correlation

1979 ◽  
Vol 57 (s5) ◽  
pp. 325s-327s ◽  
Author(s):  
E. Ambrosioni ◽  
F. Tartagni ◽  
L. Montebugnoli ◽  
B. Magnani
Keyword(s):  
Blood Pressure ◽  
Normal Subjects ◽  
Sodium Concentration ◽  
The Other ◽  
Mean Blood Pressure ◽  
Normal Blood ◽  
Normal Blood Pressure ◽  
Hypertensive Patients ◽  
Normotensive Subjects

1. Intralymphocytic sodium concentration was measured in 50 patients with essential stable hypertension, 44 patients with labile hypertension and 40 subjects with normal blood pressure. 2. Intralymphocytic sodium concentration in normotensive subjects was significantly lower than in the other two groups. 3. The concentration was significantly correlated with mean blood pressure in the group as a whole and in the groups with stable and with labile hypertension. No correlation was found in normal subjects.

scholarly journals Relationship between Coping Mechanisms to Psychosocial Stress with Blood Pressure in Young Adults: A Pilot Study

2018 ◽  
Vol 17 (3) ◽  
pp. 439-445
Author(s):  
Sithu Aung ◽  
Ramli Musa ◽  
Jamalludin Rahman A ◽  
Azarisman Shah M ◽  
Aszrin Abdullah
Keyword(s):  
Blood Pressure ◽  
Young Adults ◽  
Pilot Study ◽  
Body Mass ◽  
Psychosocial Stress ◽  
Plasma Cortisol ◽  
Normal Blood ◽  
Normal Blood Pressure ◽  
Cortisol Levels ◽  
The Relationship

Background: Hypertension is a major risk factor for cardiovascular diseases, one of the leading causes of death worldwide. Prehypertension is a precursor of hypertension, with high prevalence in young adults. Psychosocial stress can be one of the modifiable risk factors for hypertension. The objective of the study is to assess the relationship between coping mechanism to psychosocial stress and the blood pressure of young adults.Methods: A comparative crosssectional pilot study was conducted in 36 young adults aged less than 45 years. The pulse rate, blood pressure and body mass index of the respondents were recorded. Psychosocial stress was measured by plasma cortisol following an acute mental stress test; and the level of stress and the coping strategies to stress were assessed using validated Malay Version questionnaires: DASS- 21 and Brief R-COPE questionnaires.Results: Forty four percent of subjects had normotension and fifty six percent had above-normal blood pressure (prehypertension and mild hypertension). Subjects with above-normal blood pressure were associated with high plasma cortisol levels (p = 0.032), and high body mass indexes (BMI) (p = 0.004). Maladaptive coping strategy was found to be associated with high stress scores (p = 0.019). The relationship between stress and cortisol was not significant, though a higher cortisol level was noted in the high blood pressure group.Conclusion: In conclusion, higher cortisol levels and greater BMIs were associated with above-normal blood pressure in young adults. These findings are useful for future research in this area, and the continuation of this study will hopefully yield a more significant relationship.Bangladesh Journal of Medical Science Vol.17(3) 2018 p.439-445

scholarly journals Protective Effect of Gynura Bicolor Alcohol Extracts on Rat Kidney Epithelial NRK-52E Cells Induced by Tert-Butyl Hydroperoxide

2020 ◽  
Author(s):  
Shu-Ling Hsieh ◽  
Chia-Hsuan Wu ◽  
Jyh-Jye Wang ◽  
Chien-Chun Li ◽  
Chih-Chung Wu
Keyword(s):  
Cell Damage ◽  
Rat Kidney ◽  
Thiobarbituric Acid ◽  
Experimental Models ◽  
Thiobarbituric Acid Reactive Substances ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Tnf Α ◽  
Gynura Bicolor

Abstract Background: Gynura bicolor is a common vegetable in the Asia and has many physiological effects. We aimed to investigate the renoprotective effects of G. bicolor alcohol extracts (GBAEs). Methods: Rat kidney epithelial NRK-52E cell culture and kidney damage induction by tert-butyl hydroperoxide (t-BHP) were used as experimental models. In this study, the antioxidative and anti-inflammatory activities, the levels of apoptosis and apoptosis-related molecules were analyzed in NRK-52E cells following t-BHP-induced injury. Results: The results showed the levels of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) decreased by levels by 61-71% and 41-59%, respectively, in NRK-52E cells treated with 0.5, 1, or 5 mg/ml GBAE combined with t-BHP compared with t-BHP-induced cells (p<0.05). The GSH levels and GSH-to-GSSG ratio were increased in NRK-52E cells treated with 0.5, 1, and 5 mg/ml GBAE combined with t-BHP compared to t-BHT-induced cells (p<0.05). The nitrite (NO), prostaglandin E2 (PGE2), IL-6, and TNF-α levels of NRK-52E cells treated with 0.5, 1, and 5 mg/ml GBAE combined with t-BHP were decreased compared with t-BHP-induced cells (p<0.05). In addition, Bcl-2 levels were increased after treatment with 5 mg/ml GBAE combined with t-BHP, and the levels of Bax and poly (ADP-ribose) polymerase (PARP) and the apoptosis percentage were decreased after treatment with 1 or 5 mg/ml GBAE combined with t-BHP induction compared to after t-BHP induction alone (p<0.05). Conclusions: These data showed that 5 mg/ml GBAE can protect against kidney cell damage induced by t-BHP. The mechanism involves reducing cell damage and apoptosis through decreasing oxidative stress and inflammation.

Biochemical Correlates of the Increase in Blood Pressure with Age

1978 ◽  
Vol 55 (s4) ◽  
pp. 377s-379s ◽  
Author(s):  
C. E. Grim ◽  
M. H. Weinberger ◽  
D. P. Henry ◽  
F. C. Luft ◽  
N. S. Fineberg
Keyword(s):  
Blood Pressure ◽  
Plasma Renin Activity ◽  
Sodium Intake ◽  
Plasma Renin ◽  
Normal Subjects ◽  
Renin Activity ◽  
Multiple Correlation Analysis ◽  
Normotensive Subjects ◽  
Relationship Of ◽  
The Relationship

1. Blood pressure, plasma renin activity, plasma aldosterone, urinary noradrenaline during sleep (UNA-S) and several estimates of sodium intake were determined in 379 normotensive subjects (age 13–70) to investigate the relationship of these variables to blood pressure. 2. Blood pressure was correlated with age, weight, plasma renin activity UNA-S, and estimates of sodium intake. These variables were frequently intercorrelated. 3. Multiple-correlation analysis revealed that after removal of the effects of age, blood pressure was related to weight, plasma renin activity, UNA-S and estimates of sodium intake. 4. However, multiple-regression analysis failed to demonstrate an effect of plasma renin activity, UNA-S, or estimates of sodium intake on blood pressure when the effects of age, weight, race and sex were removed. 5. Careful matching of subjects by age, weight, race and sex in studies of blood pressure and biochemical factors in normal subjects is crucial to proper interpretation of such data.

The Correlation of Anxiety, Dyslipidemia and Oxidative stress in Prehypertensive and Hypertensive patients

2020 ◽  
Vol 11 (2) ◽  
pp. 2614-2619
Author(s):  
Elsa Mathew ◽  
Mukkadan J. K.
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Trait Anxiety ◽  
Normal Blood ◽  
Control Group ◽  
National Committee ◽  
Normal Blood Pressure ◽  
Hypertensive Patients ◽  
Normotensive Subjects ◽  
And Oxidative Stress

Studies have evaluated that high blood pressure kills nine million people annually Persistent. Psychological factors can be considered as a primary threat to the increase of hypertension. It may lead to cardiovascular disease, stroke and kidney disease. The current work was conducted to analyze the disparity of anxiety, dyslipidemia and oxidative stress in pre-hypertensive and hypertensive subjects. This was a cross-sectional study conducted among 180 subjects. Based on the Joint National Committee 8 Criteria, participants were divided into hypertensive patients (n₌60) and pre-hypertensive patients (n₌63). Fifty-seven healthy subjects with normal blood pressure were served as the control group. Anthropometric measurements and blood pressure were measured using the standard procedure. The biochemical parameters for measuring oxidative stress, blood glucose levels, and lipid profile were estimated. Anxiety level was assessed with the State-trait anxiety inventory (STAI) questionnaire. It is observed that the serum MalonDiAldehyde (MDA) levels (nmol/ml) were significantly higher in pre-hypertensive (3.74±0.33) and hypertensive (4.7±0.38) compared to normotensive subjects (3.05±0.38). The Superoxide Dismutase (SOD) activity (U/ml) was higher in subjects with normal blood pressure (12.67±2.31) than pre-hypertensive (11.16±2.43) and hypertensive subjects (8.98±2.32). The MDA had a significant positive correlation, and SOD had a negative association with waist-hip ratio, systolic blood pressure, diastolic blood pressure, fasting blood sugar, high-density lipoprotein, and state and trait anxiety. The present study confirmed that pre-hypertensive and hypertensive subjects suffered from more oxidative stress than normotensive subjects.

Treatment of Hypertension Induces a Fall in Platelet Basal Cytoplasmic Calcium Concentration without Influencing Platelet Aggregation

1992 ◽  
Vol 68 (06) ◽  
pp. 683-686 ◽  
Author(s):  
J Wilson ◽  
M A Orchard ◽  
C R M Prentice ◽  
A D Lees ◽  
M W Baig ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Platelet Aggregation ◽  
Calcium Antagonist ◽  
Calcium Concentration ◽  
Venous Blood ◽  
Treatment Phase ◽  
Cytoplasmic Calcium ◽  
Hypertensive Patients ◽  
Treatment Of Hypertension ◽  
Normotensive Subjects

SummaryThe relationship between blood pressure and platelet basal cytoplasmic calcium concentration ([Ca2+]i) and platelet sensitivity to aggregating agents in hypertension has been investigated in hypertensive patients and normotensive subjects. Ten severely hypertensive patients whose blood pressures were poorly controlled with metoprolol, were given calcium antagonist (either nifedipine or felodipine) as a second line agent. Venous blood samples were collected at each treatment phase for measurement, in whole blood, of platelet aggregation in response to ADP and collagen, and of basal [Ca2+]i using fura-2. Control of blood pressure by the combination of metroprolol and a calcium antagonist induced a significant decrease in median [Ca2+]i from 116 (76–181) to 73 (60–83) nM, which was similar to the median value of 70 (61–80) nM obtained in 14 normotensive subjects. Overall [Ca2+]i correlated with mean blood pressure (r = 0.51). Treatment of hypertension with calcium antagonist did not change the response of platelets to collagen or ADP. The results confirm that effective treatment of hypertension significantly reduced basal [Ca2+]i in platelets but raise doubts whether elevated basal [Ca2+]i is necessarily the sole mechanism by which the sensitivity of platelets to aggregatory agents is increased in hypertension.

scholarly journals Redox State and Lysosomal Activity in Women with Ovarian Cancer with Tumor Recurrence and Multiorgan Metastasis

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4039
Author(s):  
Paweł Sutkowy ◽  
Jolanta Czuczejko ◽  
Bogdan Małkowski ◽  
Karolina Szewczyk-Golec ◽  
Rita Łopatto ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cathepsin D ◽  
Venous Blood ◽  
Plasma Concentrations ◽  
Prostaglandin F2α ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Arylsulfatase Activity ◽  
Total Antioxidant ◽  
Cathepsin D Activity

The aim of the study is to evaluate oxidant–antioxidant balance as well as lysosomal and anti-protease activities in ovarian cancer since it has been emphasized that the crucial inducing factor of carcinogenesis may be reactive oxygen/nitrogen species or, more precisely, oxidative stress-induced inflammation. The study involved 15 women with ovarian cancer, aged 59.9 ± 7.8 years, and 9 healthy women aged 56.3 ± 4.3 years (controls). The study material was venous blood collected from fasting subjects. In erythrocytes, the activities of superoxide dismutase, glutathione peroxidase, and catalase, as well as concentrations of conjugated dienes (CDs) and thiobarbituric acid reactive substances (TBARS), were investigated. CD, TBARS, and vitamins A and E plasma concentrations were also determined. Moreover, total antioxidant capacity and concentrations of 4-hydroxynonenal adducts and 8-iso-prostaglandin F2α, as well as activities of acid phosphatase, arylsulfatase, cathepsin D, and α1-antitrypsin, were studied in serum. The vitamin E and 8-iso-prostaglandin F2α concentrations as well as arylsulfatase activity were lower in the women with cancer compared to the controls (p = 0.006, p = 0.03, p = 0.001, respectively). In contrast, cathepsin D activity was lower in the controls (p = 0.04). In the peripheral blood of the women with cancer, oxidant–antioxidant and lysosomal disturbances were observed.

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