Dynamic, non-contact 3D sample rotation for microscopy

Mapping Intimacies ◽

10.1101/177733 ◽

2017 ◽

Author(s):

Frederic Berndt ◽

Gopi Shah ◽

Jan Brugués ◽

Jan Huisken

Keyword(s):

Magnetic Field ◽

Magnetic Beads ◽

Light Sheet ◽

Optimal Performance ◽

Fluorescence Microscope ◽

Dynamic Adaptation ◽

Sample Rotation ◽

Sample Orientation

AbstractIn vivo imaging of growing and developing samples requires a dynamic adaptation of the sample orientation to continuously achieve optimal performance. Here, we present how, after the injection of magnetic beads, a sample can be freely positioned by applying a magnetic field. We demonstrate its performance for zebrafish on an epi-fluorescence microscope and on a light sheet system for superior multi-view acquisition.

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Spectroscopic imaging of human disease

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166889 ◽

1996 ◽

Vol 54 ◽

pp. 884-885

Author(s):

D.J. Meyerhoff

Keyword(s):

Magnetic Field ◽

Magnetic Resonance ◽

Field Gradient ◽

Human Disease ◽

Morphological Changes ◽

Magnetic Field Gradient ◽

Spectroscopic Imaging ◽

Resonance Spectroscopy ◽

Tissue Water

Magnetic Resonance Imaging (MRI) observes tissue water in the presence of a magnetic field gradient to study morphological changes such as tissue volume loss and signal hyperintensities in human disease. These changes are mostly non-specific and do not appear to be correlated with the range of severity of a certain disease. In contrast, Magnetic Resonance Spectroscopy (MRS), which measures many different chemicals and tissue metabolites in the millimolar concentration range in the absence of a magnetic field gradient, has been shown to reveal characteristic metabolite patterns which are often correlated with the severity of a disease. In-vivo MRS studies are performed on widely available MRI scanners without any “sample preparation” or invasive procedures and are therefore widely used in clinical research. Hydrogen (H) MRS and MR Spectroscopic Imaging (MRSI, conceptionally a combination of MRI and MRS) measure N-acetylaspartate (a putative marker of neurons), creatine-containing metabolites (involved in energy processes in the cell), choline-containing metabolites (involved in membrane metabolism and, possibly, inflammatory processes),

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Intense Static Magnetic Field Induced Bone Growth in Vivo

Electromagnetic Biology and Medicine ◽

10.3109/15368378409035968 ◽

1984 ◽

Vol 3 (1) ◽

pp. 223-234

Author(s):

Frank Papatheofanis ◽

Bill Fapatheofanls ◽

Robert Ray

Keyword(s):

Magnetic Field ◽

Static Magnetic Field ◽

Bone Growth

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Magnetic Nanodiscs—A New Promising Tool for Microsurgery of Malignant Neoplasms

Nanomaterials ◽

10.3390/nano11061459 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1459

Author(s):

Tatiana N. Zamay ◽

Vladimir S. Prokopenko ◽

Sergey S. Zamay ◽

Kirill A. Lukyanenko ◽

Olga S. Kolovskaya ◽

...

Keyword(s):

Magnetic Field ◽

Remote Control ◽

Tumor Cells ◽

Biological Effects ◽

Malignant Neoplasms ◽

Rotating Magnetic Fields ◽

Magnetic Structures ◽

Promising Tool

Magnetomechanical therapy is one of the most perspective directions in tumor microsurgery. According to the analysis of recent publications, it can be concluded that a nanoscalpel could become an instrument sufficient for cancer microsurgery. It should possess the following properties: (1) nano- or microsized; (2) affinity and specificity to the targets on tumor cells; (3) remote control. This nano- or microscalpel should include at least two components: (1) a physical nanostructure (particle, disc, plates) with the ability to transform the magnetic moment to mechanical torque; (2) a ligand—a molecule (antibody, aptamer, etc.) allowing the scalpel precisely target tumor cells. Literature analysis revealed that the most suitable nanoscalpel structures are anisotropic, magnetic micro- or nanodiscs with high-saturation magnetization and the absence of remanence, facilitating scalpel remote control via the magnetic field. Additionally, anisotropy enhances the transmigration of the discs to the tumor. To date, four types of magnetic microdiscs have been used for tumor destruction: synthetic antiferromagnetic P-SAF (perpendicular) and SAF (in-plane), vortex Py, and three-layer non-magnetic–ferromagnet–non-magnetic systems with flat quasi-dipole magnetic structures. In the current review, we discuss the biological effects of magnetic discs, the mechanisms of action, and the toxicity in alternating or rotating magnetic fields in vitro and in vivo. Based on the experimental data presented in the literature, we conclude that the targeted and remotely controlled magnetic field nanoscalpel is an effective and safe instrument for cancer therapy or theranostics.

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In-vivo 3D imaging of Zebrafish’s intersegmental vessel development by a bi-directional light-sheet illumination microscope

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2021.03.160 ◽

2021 ◽

Vol 557 ◽

pp. 8-13

Author(s):

Xiaofei Qin ◽

Chong Chen ◽

Linbo Wang ◽

Xiaohu Chen ◽

Yong Liang ◽

...

Keyword(s):

3D Imaging ◽

Light Sheet ◽

Vessel Development

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Enhancement and Segmentation Workflow for the Developing Zebrafish Vasculature

Journal of Imaging ◽

10.3390/jimaging5010014 ◽

2019 ◽

Vol 5 (1) ◽

pp. 14 ◽

Cited By ~ 1

Author(s):

Elisabeth Kugler ◽

Karen Plant ◽

Timothy Chico ◽

Paul Armitage

Keyword(s):

Open Source Software ◽

Light Sheet ◽

Visual Assessment ◽

Cardiovascular Development ◽

Fluorescent Reporter ◽

Enhancement Method ◽

Vertebrate Model ◽

Vascular Enhancement ◽

Light Sheet Fluorescence Microscopy

Zebrafish have become an established in vivo vertebrate model to study cardiovascular development and disease. However, most published studies of the zebrafish vascular architecture rely on subjective visual assessment, rather than objective quantification. In this paper, we used state-of-the-art light sheet fluorescence microscopy to visualize the vasculature in transgenic fluorescent reporter zebrafish. Analysis of image quality, vascular enhancement methods, and segmentation approaches were performed in the framework of the open-source software Fiji to allow dissemination and reproducibility. Here, we build on a previously developed image processing pipeline; evaluate its applicability to a wider range of data; apply and evaluate an alternative vascular enhancement method; and, finally, suggest a work-flow for successful segmentation of the embryonic zebrafish vasculature.

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Magnetic targeting with superparamagnetic iron oxide nanoparticles for in vivo glioma

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0101 ◽

2017 ◽

Vol 6 (5) ◽

pp. 449-472 ◽

Cited By ~ 4

Author(s):

Marina Fontes de Paula Aguiar ◽

Javier Bustamante Mamani ◽

Taylla Klei Felix ◽

Rafael Ferreira dos Reis ◽

Helio Rodrigues da Silva ◽

...

Keyword(s):

Magnetic Field ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Superparamagnetic Iron Oxide ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Process Efficiency ◽

Cochrane Library ◽

Oxide Nanoparticles ◽

Magnetic Targeting

AbstractThe purpose of this study was to review the use of the magnetic targeting technique, characterized by magnetic driving compounds based on superparamagnetic iron oxide nanoparticles (SPIONs), as drug delivery for a specific brain locus in gliomas. We reviewed a process mediated by the application of an external static magnetic field for targeting SPIONs in gliomas. A search of PubMed, Cochrane Library, Scopus, and Web of Science databases identified 228 studies, 23 of which were selected based on inclusion criteria and predetermined exclusion criteria. The articles were analyzed by physicochemical characteristics of SPIONs used, cell types used for tumor induction, characteristics of experimental glioma models, magnetic targeting technical parameters, and analysis method of process efficiency. The study shows the highlights and importance of magnetic targeting to optimize the magnetic targeting process as a therapeutic strategy for gliomas. Regardless of the intensity of the patterned magnetic field, the time of application of the field, and nanoparticle used (commercial or synthesized), all studies showed a vast advantage in the use of magnetic targeting, either alone or in combination with other techniques, for optimized glioma therapy. Therefore, this review elucidates the preclinical and therapeutic applications of magnetic targeting in glioma, an innovative nanobiotechnological method.

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R154: Plateforme de microscopie à feuille de lumière (Light Sheet based Fluorescence Microscope) : une nouvelle méthode pour explorer le vivant en 3D

Bulletin du Cancer ◽

10.1016/s0007-4551(15)31075-4 ◽

2010 ◽

Vol 97 (4) ◽

pp. S77

Author(s):

C. Lorenzo ◽

V. Lobjois ◽

G. Gay ◽

F. DeVielleville ◽

A. Maandhui ◽

...

Keyword(s):

Light Sheet ◽

Fluorescence Microscope

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Effect of Magnetic Field on Bone Healing around Endosseous Implants – An In-vivo Study

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2016/21509.8666 ◽

2016 ◽

Cited By ~ 1

Author(s):

Mahalakshmi Gujjalapudi

Keyword(s):

Magnetic Field ◽

Bone Healing ◽

In Vivo Study ◽

Endosseous Implants

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The Research of a New Method for Manipulating Magnetic Beads through Multi-Layered Flat Micro-Coils Coupled with Permanent Magnet

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.753-755.1571 ◽

2013 ◽

Vol 753-755 ◽

pp. 1571-1575

Author(s):

Zhi Hua Liu ◽

Yu Feng Huang ◽

Jian Peng Li ◽

Xin Wei Xu

Keyword(s):

Magnetic Field ◽

Permanent Magnet ◽

Flow Velocity ◽

Magnetic Beads ◽

Magnetic Bead ◽

New Method ◽

Viscous Resistance ◽

Main Factors ◽

The Magnetic Field

Magnetic bead droplet's non-contacted manipulation can be realized in Electromagnetic MEMS, but how to achieve magnetic beads manipulation is the major problem. A new method of multi-layered flat coils coupled with permanent magnet was proposed. Firstly, the theory of magnetic bead manipulation was analyzed and the main factors affected the magnetic beads manipulation was identified; then the magnetic field of multi-layered flat coils and Stokes viscous resistance of magnetic beads were analyzed and simulated quantificationally; finally the magnetic bead capture area was got under different flow velocity. Consequently the feasibility and correctness of this method was verified.

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