scholarly journals The Potential of Lipolytic Filamentous Fungi Isolated from Landfill Soil as Poly-β-Hydroxybutirate (PHB) Bioplastic Degrader

2019 ◽  
Author(s):  
Nur Arfa Yanti ◽  
Endang Sriwahyuni ◽  
Nur Rayani La Omi ◽  
Nurhayani H. Muhiddin ◽  
Sitti Wirdhana Ahmad
Keyword(s):  
Aspergillus Terreus ◽  
Agar Medium ◽  
Clear Zone ◽  
Ability Test ◽  
Spore Form ◽  
Phenotypic Characters ◽  
Asexual Spore ◽  
Spore Mass ◽  
Two Stages

AbstractThe present study was investigated the potential of lipolytic fungi (molds) isolated from landfill soil in degrading Poly-β-hydroxybutyrate (PHB). Screening of PHB-degrading lipolytic molds was done in two stages, such as screening of lipolytic molds which was identified by the formation of orange fluorescent halos around the colony on rhodamine B agar medium and the degradation PHB ability test was identified by the formation of clear zone around colony on PHB emulsion medium. Characterization of isolates was done based on phenotypic characters and the identification was done by numerical-phenetic analysis. Three lipolytic mold isolates that have ability in degrading PHB bioplastic i.e isolate KC1, KE1 and KE6. These molds have asexual spore form conidia, foot cell, septate hyphae, unbranched conidiophore, and spore mass located at the apex of phialid. The identification results showed that isolate KC1 is identic to Aspergillus terreus, KE1 is identic to Aspergillus niger and KE6 is identic to Aspergillus fumigatus.

scholarly journals Production and Characterization of Extracellular Protease from Bacillus sp. 140-B Isolated from Pineapple Plantation in Lampung, Indonesia

2017 ◽  
Vol 3 (4) ◽  
pp. 170
Author(s):  
Nur Laili ◽  
Sarjiya Antonius
Keyword(s):  
Protease Activity ◽  
Extracellular Enzymes ◽  
Agar Medium ◽  
Skim Milk ◽  
Bacillus Sp ◽  
Enzyme Properties ◽  
Clear Zone ◽  
Optimum Ph ◽  
Effects Of Temperature

<p class="Els-Abstract-text"><em>Bacillus </em>sp<em>.</em> being industrially important organisms produces a wide variety of extracellular enzymes including protease. <em>Bacillus </em>sp. 140-B isolated from rhizosphere area of pineapple plantation in Lampung Province, Indonesia was tested for production of protease on skim milk agar medium. The aims of this study were to characterize and investigate some properties of  protease activity from <em>Bacillus </em>sp<em>. </em>140-B. <em>Bacillus </em>sp<em>. </em>140-B showed  protease activity qualitatively by clear zone diameter of 15.0 mm and the highest protease activity was 35.02 Unit · mg<strong><sup>–1</sup></strong> protein at 14 h after incubation. Some properties of protease activities from <em>Bacillus </em>sp<em>. </em>140-B including effects of temperature, pH and several metals were observed in this experiment. The protease activity from <em>Bacillus </em>sp<em>. </em>140-B had optimum pH of 7,0 and the optimum temperature was 60 °C. Several metals which were evaluated on protease activity showed that Mn could increase protease activity, while other metals (Ca, Co, and Hg) showed decreasing protease activity of <em>Bacillus </em>sp<em>. </em>140-B.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords: </strong><em>Bacillus sp.</em> 140-B; enzyme activity; enzyme properties; protease</p></div>

Growth mechanism and characterization of ZnO nano-tubes synthesized using the hydrothermal-etching method

2009 ◽  
Vol 63 (6) ◽  
Author(s):  
Yan Li ◽  
Chuan-Sheng Liu ◽  
Yun-Ling Zou
Keyword(s):  
Room Temperature ◽  
Morphological Analysis ◽  
Diffraction Field ◽  
X Ray Diffraction ◽  
X Ray ◽  
Room Temperature Photoluminescence ◽  
Etching Method ◽  
Nano Rods ◽  
Two Stages

AbstractZnO nano-tubes (ZNTs) have been successfully synthesized via a simple hydrothermal-etching method, and characterized by X-ray diffraction, field emission scanning electron microscopy and room temperature photoluminescence measurement. The as-synthesized ZNTs have a diameter of 500 nm, wall thickness of 20–30 nm, and length of 5 µm. Intensity of the plane (0002) diffraction peak, compared with that of plane (10$$ \bar 1 $$0) of ZNTs, is obviously lower than that of ZnO nano-rods. This phenomenon can be caused by the smaller cross section of plane (0002) of the nano-tubes compared with that of other morphologies. On basis of the morphological analysis, the formation process of nano-tubes can be proposed in two stages: hydrothermal growth and reaction etching process.

scholarly journals DISCOVER: A facile structure-based screening method for vinyl compound producing microbes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuji Aso ◽  
Mei Sano ◽  
Hikari Kuroda ◽  
Hitomi Ohara ◽  
Hiroshi Ando ◽  
...  
Keyword(s):  
Heck Reaction ◽  
Aspergillus Terreus ◽  
Agar Medium ◽  
Screening Method ◽  
Coupling Reactions ◽  
Ene Reaction ◽  
Vinyl Compound ◽  
Novel Structure ◽  
Iodine Test ◽  
Microbial Screening

Abstract Here we report a novel structure-based microbial screening method for vinyl compound discovery, DISCOVER (direct screening method based on coupling reactions for vinyl compound producers). Through a two-step screening procedure based on selective coupling reactions of terminal alkenes, the thiol-ene reaction (1st step of screening) and Mizoroki-Heck reaction, followed by iodine test (2nd step of screening), microbes producing vinyl compounds like itaconic acid (IA) can be isolated from soil samples. In the 1st step of screening, soil sources are plated on agar medium supplemented with an antimicrobial agent, α-thioglycerol (TG), and a radical initiator, VA-044 (VA). In the 2nd step of screening, vinyl compounds produced in the cultures are labelled with iodobenzene via the Mizoroki-Heck reaction, followed by an iodine test, leading to the detection and characterisation of labelled products. We evaluated the validity of DISCOVER using IA and its producer Aspergillus terreus. Experimental data supported our hypothesis that IA reacts with TG in the medium via the thiol-ene reaction and consequently, A. terreus rapidly forms colonies on the agar medium because of the loss of the antimicrobial activity of TG. Using DISCOVER, high throughput and selective isolation of A. terreus strains producing IA was possible from soils.

Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus

2018 ◽  
Vol 42 (5) ◽  
pp. e12551 ◽  
Author(s):  
Maíra N. de Almeida ◽  
Valéria M. Guimarães ◽  
Daniel L. Falkoski ◽  
Brenda R. de Camargo ◽  
Gizele C. Fontes-Sant'ana ◽  
...  
Keyword(s):  
Aspergillus Terreus ◽  
Purification And Characterization

scholarly journals Production and Characterization of Activated Carbon from Coal for Gold Adsorption in Cyanide Solutions

2020 ◽  
Vol 40 (1) ◽  
pp. 34-44
Author(s):  
Karen L. Martínez-Mendoza ◽  
Juan Manuel Barraza Burgos ◽  
Nilson Marriaga-Cabrales ◽  
Fiderman Machuca-Martinez ◽  
Mariber Barajas ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Activated Carbons ◽  
Physical Activation ◽  
Adsorption Model ◽  
Waste Solution ◽  
Synthetic Solution ◽  
Cyanide Solutions ◽  
Two Stages ◽  
Characterization Of Activated Carbon

In this work, activated carbons were produced using coal as raw matter from seven Colombian carboniferous zones. Physical activation was performed in two stages: a carbonization stage with Nitrogen at a temperature of 850 °C and a residence time of 2 h, followed by an activation stage using steam at temperatures of 700 and 850 °C with residence times of 1,5 h and 2,5 h. From the pore volume characterization for the adsorption of gold, two activated carbons from Cundinamarca, obtained at 850 °C (1,5 h), 850 °C (2,5 h), and a commercial carbon (GRC 22) were selected. Gold adsorption tests were performed with those three activated carbons using synthetic aurocyanide solutions and a gold waste solution. The data of the adsorption isotherms were adjusted using the Freundlich adsorption model for the synthetic solution, as well as Langmuir for the waste solution. The results showed that, using a solution of 1 ppm, the activated carbons C-850-2.5 and C- 850-1.5 produced the higher maximum gold loading capacities in the equilibrium (8,7 and 9,3 mg Au/g, respectively) in comparison to the commercial activated carbon (4,7 mg Au/g).  Gold adsorption test using a waste solution (21 ppm of gold) showed that the activated carbon C-850-1.5 had the highest value of adsorption capacity (4,58 mg Au/g) compared to C-850-2.5 (2,95 mgAu /g).

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