Preparation of cationized albumin nanoparticles loaded indirubin by high pressure hemogenizer

Indirubin can be applied as an anti-cancer drug for inhibition of brain tumors. However, its performance is reduced due to hydrophobicity. In this study, we synthesized cationic human serum albumin (CHSA) nanoparticle by a new hybrid approach for improvement the surface chemistry of albumin and investigate the amount of indirubin loaded CHSA nanoparticle. In this study, the generated mechanical force from a high-pressure homogenizer (HPH) was used to make nanoparticles with a certain size with narrow polydispersity. The results indicated that the size of indirubin loaded CHSA nanoparticles were 130 nm and their zeta potential were +9. Besides, the encapsulation efficiency and drug loading capacity were found to be 85% and 5.8 %, respectively. To the best to our knowledge, this is the first time that indirubin has been used in albumin nanoparticles. In this study, indirubin loaded CHSA nanoparticles was shown can be a potential candidate for drug delivery in the treatment of glioblastoma. Moreover, the cationized form allows the chemical agent to be transmitted to the brain.

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Enhanced hydroxyapatite nanorods formation on graphene oxide nanocomposite as a potential candidate for protein adsorption, pH controlled release and an effective drug delivery platform for cancer therapy

Analytical Methods ◽

10.1039/c6ay02348g ◽

2017 ◽

Vol 9 (2) ◽

pp. 240-252 ◽

Cited By ~ 22

Author(s):

G. Bharath ◽

B. Swarna Latha ◽

Edreese H. Alsharaeh ◽

P. Prakash ◽

N. Ponpandian

Keyword(s):

Drug Delivery ◽

Graphene Oxide ◽

Drug Loading ◽

Creatine Phosphate ◽

Cancer Drug ◽

Potential Candidate ◽

Anti Cancer ◽

Delivery Platform ◽

Phosphorus Source ◽

Ph Dependent

Creatine phosphate used as a phosphorus source for synthesis of a HAp/GO nanocomposite toward protein/anti-cancer drug loading and selective pH dependent drug delivery platforms.

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Stereocomplexation Assisted Assembly of Poly(γ-glutamic Acid)-graft-polylactide Nano-micelles and Their Efficacy as Anticancer Drug Carrier

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520617666170911170104 ◽

2018 ◽

Vol 18 (2) ◽

pp. 302-311

Author(s):

Shulin Dai ◽

Yucheng Feng ◽

Shuyi Li ◽

Yuxiao Chen ◽

Meiqing Liu ◽

...

Keyword(s):

Glutamic Acid ◽

Drug Carrier ◽

Drug Loading ◽

Drug Carriers ◽

Cancer Drug ◽

Drug Release Profile ◽

Sustained Drug Release ◽

Anti Cancer ◽

Physical Interactions

Background: Micelles as drug carriers are characterized by their inherent instability due to the weak physical interactions that facilitate the self-assembly of amphiphilic block copolymers. As one of the strong physical interactions, the stereocomplexation between the equal molar of enantiomeric polylactides, i.e., the poly(L-lactide) (PLLA) and poly(D-lactide) (PDLA), may be harnessed to obtain micelles with enhanced stability and drug loading capacity and consequent sustained release. </P><P> Aims/Methods: In this paper, stereocomplexed micelles gama-PGA-g-PLA micelles) were fabricated from the stereocomplexation between poly(gama-glutamic acid)-graft-PLLA gama-PGA-g-PLA) and poly(gamaglutamic acid)-graft-PDLA gama-PGA-g-PLA). These stereocomplexed micelles exhibited a lower CMC than the corresponding enantiomeric micelles. Result: Furthermore, they showed higher drug loading content and drug loading efficiency in addition to more sustained drug release profile in vitro. In vivo imaging confirmed that the DiR-encapsulated stereocomplexed gama-PGA-g-PLA micelles can deliver anti-cancer drug to tumors with enhanced tissue penetration. Overall, gama-PGA-g-PLA micelles exhibited greater anti-cancer effects as compared with the free drug and the stereocomplexation may be a promising strategy for fabrication of anti-cancer drug carriers with significantly enhanced efficacy.

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Nanoparticles based on retinoic acid caped with ferrocenium: a novel synthesized targetable nanoparticle both with anti-cancer effect and drug loading capacity

RSC Advances ◽

10.1039/c9ra02472g ◽

2019 ◽

Vol 9 (28) ◽

pp. 16208-16214 ◽

Cited By ~ 1

Author(s):

Yibo Wang ◽

Bin Zhao ◽

Lu Wang ◽

Wenhuan Bu ◽

Shuwei Liu ◽

...

Keyword(s):

Retinoic Acid ◽

Drug Loading ◽

Cancer Drug ◽

Loading Capacity ◽

Anti Cancer

A retinoic acid nanoparticle with the ability of carrying a second anti-cancer drug, taxol, was developed. The anti-cancer nanoparticles were shown to have a better application prospect than that of RA or taxol alone.

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Reducing cytotoxicity while improving anti-cancer drug loading capacity of polypropylenimine dendrimers by surface acetylation

Acta Biomaterialia ◽

10.1016/j.actbio.2012.07.031 ◽

2012 ◽

Vol 8 (12) ◽

pp. 4304-4313 ◽

Cited By ~ 28

Author(s):

Fei Wang ◽

Xiaopan Cai ◽

Yunzhang Su ◽

Jingjing Hu ◽

Q. Wu ◽

...

Keyword(s):

Drug Loading ◽

Cancer Drug ◽

Loading Capacity ◽

Anti Cancer ◽

Surface Acetylation

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Isolation and Anti-Leukemic Characterization of Extracellular L-asparaginase From Endophytic Bacterium, Brevibacterium sp. M-R21 Isolated Glycyrrhiza glabra Root

Biointerface Research in Applied Chemistry ◽

10.33263/briac112.91139125 ◽

2020 ◽

Vol 11 (2) ◽

pp. 9113-9125

Keyword(s):

Cell Line ◽

Leukemia Cell ◽

Glycyrrhiza Glabra ◽

Leukemia Cell Line ◽

Leukemia Cells ◽

Normal Lung ◽

Cancer Drug ◽

Lung Cells ◽

Potential Candidate ◽

Anti Cancer

L-Asparaginase (L-ASPase) is known as a potent anti-cancer drug against L-Asparagine-auxotroph tumor cells. In this study, an endophytic L-ASPase producing bacterium of the genus Bervibacillus from the root of Glycyrrhiza glabra was screened and characterized. After purification of the enzyme by ammonium sulfate precipitation, dialysis, and silica gel column chromatography, anti-cancer studies were performed against MRC-5 (normal lung cells) and U937 cell (leukemia cell line). Additionally, optimization fermentation was performed in terms of significant variables screened from a one-factor-at-the-time (OFAT) approach. The interactions of different experimental parameters were investigated using the response surface methodology (RSM) with the central composite design (CCD) algorithm. Cytotoxicity study showed that the dose-dependent effect of the L-ASPase at 100 IU/ml had a lethality of about 80% against leukemia cells. Therefore, the IC50 of the enzyme for leukemia cells was calculated to be approximately 33.54 IU/ml. Interestingly, the cytotoxicity of L-ASPase against normal lung cells was only about 20% at L-ASPase activity of 60-100 IU/ml. Based on the quadratic model, the optimal fermentation conditions were predicted to be 2% glucose, 2% NaCl, pH7, and incubation temperature 30 °C. Under these conditions, the highest enzyme activity was 90 IU/ml, which had an efficiency of about 30% compared to non-optimized conditions. The results showed that L-ASPase isolated from Brevibacterium sp. M-R21 with selective cytotoxicity against the leukemia cell line may be a potential candidate as an anti-cancer drug after further study.

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Lensoside Aβ as an Adjuvant to the Anti-Glioma Potential of Sorafenib

Cancers ◽

10.3390/cancers13112637 ◽

2021 ◽

Vol 13 (11) ◽

pp. 2637

Author(s):

Aleksandra Maciejczyk ◽

Justyna Kapral-Piotrowska ◽

Joanna Sumorek-Wiadro ◽

Adrian Zając ◽

Ewa Grela ◽

...

Keyword(s):

Cell Death ◽

Caspase 3 ◽

Kinase Inhibitor ◽

Morphological Changes ◽

Potential Candidate ◽

Membrane Structures ◽

Raf Kinase ◽

Programmed Death ◽

Anti Cancer ◽

First Time

Aim: The anti-glioma effect of lensoside Aβ alone and in combination with sorafenib (pro-survival Raf kinase inhibitor) was evaluated for the first time in terms of programmed cell death induction in anaplastic astrocytoma and glioblastoma multiforme cell lines as an experimental model. Apoptosis, autophagy, and necrosis were identified microscopically (fluorescence and scanning microscopes) and confirmed by flow cytometry (mitochondrial membrane potential MMP and cell death). The expression of apoptotic (caspase 3) and autophagic markers (beclin 1) as well as Raf kinase were estimated by immunoblotting. The FTIR method was used to determine the interaction of the studied drugs with lipid and protein groups within cells, while the modes of drug action within the cells were assessed with the FLIM technique. Results: Lensoside Aβ itself does not exhibit anti-glioma activity but significantly enhances the anti-cancer potential of sorafenib, initiating mainly apoptosis of up to 90% of cells. It was correlated with an increased level of active caspase 3, a reduced MMP value, and a lower level of Raf kinase. The interaction with membrane structures led to morphological changes typical of programmed death. Conclusions: Our results indicate that lensoside Aβ plays an important role as an adjuvant in chemotherapy with sorafenib and may be a potential candidate in anti-glioma combination therapy.

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Floxuridine-loaded silk fibroin nanospheres

RSC Advances ◽

10.1039/c4ra02113d ◽

2014 ◽

Vol 4 (35) ◽

pp. 18171-18177 ◽

Cited By ~ 21

Author(s):

Shuying Yu ◽

Wenhua Yang ◽

Sheng Chen ◽

Mengjie Chen ◽

Yezhuo Liu ◽

...

Keyword(s):

Controlled Release ◽

Silk Fibroin ◽

Drug Loading ◽

Cancer Drug ◽

Loading Capacity ◽

Anti Cancer ◽

Release Property ◽

Controllable Size

A clinical used anti-cancer drug floxuridine was successfully encapsulated in silk fibroin nanospheres. Such drug-loaded nanospheres have controllable size, fair drug-loading capacity and controlled release property, which maybe a good candidate for lymphatic chemotherapy.

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Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

Marine Drugs ◽

10.3390/md18010015 ◽

2019 ◽

Vol 18 (1) ◽

pp. 15 ◽

Cited By ~ 9

Author(s):

Van Bon Nguyen ◽

Shan-Ping Chen ◽

Thi Hanh Nguyen ◽

Minh Trung Nguyen ◽

Thi Thanh Thao Tran ◽

...

Keyword(s):

Serratia Marcescens ◽

Bioactive Compound ◽

Potential Candidate ◽

Hep G2 ◽

Anticancer Activities ◽

Anti Cancer ◽

Initial Ph ◽

Ic50 Values ◽

First Time ◽

Mcf 7

Marine chitins (MC) have been utilized for the production of vast array of bioactive products, including chitooligomers, chitinase, chitosanase, antioxidants, anti-NO, and antidiabetic compounds. The aim of this study is the bioprocessing of MC into a potent anticancer compound, prodigiosin (PG), via microbial fermentation. This bioactive compound was produced by Serratia marcescens TKU011 with the highest yield of 4.62 mg/mL at the optimal conditions of liquid medium with initial pH of 5.65–6.15 containing 1% α-chitin, 0.6% casein, 0.05% K2HPO4, and 0.1% CaSO4. Fermentation was kept at 25 °C for 2 d. Notably, α-chitin was newly investigated as the major potential material for PG production via fermentation; the salt CaSO4 was also found to play the key role in the enhancement of PG yield of Serratia marcescens fermentation for the first time. PG was qualified and identified based on specific UV, MALDI-TOF MS analysis. In the biological activity tests, purified PG demonstrated potent anticancer activities against A549, Hep G2, MCF-7, and WiDr with the IC50 values of 0.06, 0.04, 0.04, and 0.2 µg/mL, respectively. Mytomycin C, a commercial anti-cancer compound was also tested for comparison purpose, showing weaker activity with the IC50 values of 0.11, 0.1, 0.14, and 0.15 µg/mL, respectively. As such, purified PG displayed higher 2.75-fold, 1.67-fold, and 3.25-fold efficacy than Mytomycin C against MCF-7, A549, and Hep G2, respectively. The results suggest that marine chitins are valuable sources for production of prodigiosin, a potential candidate for cancer drugs.

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Gadolinium-containing polymer microspheres: a dual-functional theranostic agent for magnetic resonance imaging and cancer therapy

New Journal of Chemistry ◽

10.1039/c9nj00263d ◽

2019 ◽

Vol 43 (15) ◽

pp. 5987-5995 ◽

Cited By ~ 2

Author(s):

Xu Dong ◽

Muhammad Ali Tahir ◽

Liwu Zhang ◽

Christian G. Schäfer

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Drug Loading ◽

Cancer Drug ◽

Resonance Imaging ◽

Mri Contrast ◽

Theranostic Agent ◽

Dual Functional ◽

Acid Copolymer ◽

Anti Cancer

Preparation of poly(gadolinium methacrylate-co-methacrylic acid) copolymer microspheres with high MRI contrast efficiency and controlled anti-cancer drug loading and release capability.

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