scholarly journals Fast 3D Clear: A Fast, Aqueous-Based, Reversible Three-Day Tissue Clearing Method for Adult and Embryonic Mouse Brain and Whole Body

2021 ◽  
Author(s):  
Stylianos Kosmidis ◽  
Adrian Negrean ◽  
Alex Dranovsky ◽  
Attila Losonczy ◽  
Eric Kandel
Keyword(s):  
Light Sheet ◽  
Whole Body ◽  
Two Dimensional ◽  
Embryonic Mouse ◽  
Optical Clearing ◽  
Vast Array ◽  
High Transparency ◽  
Mouse Tissues ◽  
Wide Range ◽  
Clearing Method

To date, a variety of optical clearing methods have emerged that serve as powerful tools to study intact whole organ anatomy and neuronal circuits. Here we describe a newly developed, aqueous-based clearing protocol named Fast 3D Clear, that relies on THF for tissue dehydration/rehydration and utilizes Histodenz as clearing solution. Fast 3D Clear requires three days to achieve high transparency of adult and embryonic mouse tissues, while maintaining their anatomical integrity, and preserving a vast array of transgenic and viral/dye fluorophores, such as GCaMP3/6, tdTomato, Fast Blue, and IRF670. A unique advantage of Fast 3D Clear is its complete reversibility, and thus compatibility with tissue sectioning and immunohistochemistry. Fast 3D Clear can be easily and quickly applied to a wide range of biomedical studies, thereby greatly facilitating the acquisition of high-resolution, three- and two-dimensional images with light sheet and confocal microscopy.

scholarly journals Quantitative flow analysis around aquatic animals using laser sheet particle image velocimetry

1995 ◽  
Vol 198 (2) ◽  
pp. 283-294 ◽  
Author(s):  
E Stamhuis ◽  
J Videler
Keyword(s):  
Particle Image Velocimetry ◽  
Flow Field ◽  
Particle Image ◽  
Light Sheet ◽  
Flow Fields ◽  
Image Correlation ◽  
Dissipated Energy ◽  
Two Dimensional ◽  
Image Velocimetry ◽  
Wide Range

Two alternative particle image velocimetry (PIV) methods have been developed, applying laser light sheet illumination of particle-seeded flows around marine organisms. Successive video images, recorded perpendicular to a light sheet parallel to the main stream, were digitized and processed to map the flow velocity in two-dimensional planes. In particle tracking velocimetry (PTV), displacements of single particles in two subsequent images were determined semi-automatically, resulting in flow diagrams consisting of non-uniformly distributed velocity vectors. Application of grid-cell averaging resulted in flow field diagrams with uniform vector distribution. In sub-image correlation PIV (SCPIV), repetitive convolution filtering of small sub-areas of two subsequent images resulted in automatic determination of cross-correlation peaks, yielding flow field diagrams with regularly spaced velocity vectors. In both PTV and SCPIV, missing values, caused by incomplete particle displacement information in some areas of the images or due to rejection of some erroneous vectors by the vector validation procedure, were interpolated using a two-dimensional spline interpolation technique. The resultant vector flow fields were used to study the spatial distribution of velocity, spatial acceleration, vorticity, strain and shear. These flow fields could also be used to test for flow in the third dimension by studying the divergence, and to detect the presence and location of vortices. The results offer detailed quantitative descriptions of the flow morphology and can be used to assess dissipated energy. The versatile character of the technique makes it applicable to a wide range of fluid mechanical subjects within biological research. So far it has been successfully applied to map the flow around swimming copepods, fish larvae and juvenile fish and the ventilation current of a tube-living shrimp.

scholarly journals A Fast, Aqueous, Reversible Three-Day Tissue Clearing Method for Adult and Embryonic Mouse Brain and Whole Body

2021 ◽  
pp. 100090
Author(s):  
Stylianos Kosmidis ◽  
Adrian Negrean ◽  
Alex Dranovsky ◽  
Attila Losonczy ◽  
Eric R. Kandel
Keyword(s):  
Mouse Brain ◽  
Whole Body ◽  
Embryonic Mouse ◽  
Tissue Clearing ◽  
Clearing Method

Age- and sex-related bone uptake of Tc-99m-HDP measured by whole-body bone scanning

2000 ◽  
Vol 39 (05) ◽  
pp. 127-132 ◽  
Author(s):  
Nicole Sieweke ◽  
K. H. Bohuslavizki ◽  
W. U. Kampen ◽  
M. Zuhayra ◽  
M. Clausen ◽  
...  
Keyword(s):  
Whole Body ◽  
Bone Lesions ◽  
Men And Women ◽  
Bone Uptake ◽  
Normal Bone ◽  
Age Related ◽  
Number Of Patients ◽  
Wide Range ◽  
Bone Scans ◽  
Body Bone

Summary Aim of this study was to validate a recently introduced new and easy-to-perform method for quantifying bone uptake of Tc-99m-labelled diphosphonate in a routine clinical setting and to establish a normal data base for bone uptake depending on age and gender. Methods: In 49 women (14-79 years) and 47 men (6-89 years) with normal bone scans as well as in 49 women (33-81 years) and 37 men (27-88 years) with metastatic bone disease whole-body bone scans were acquired at 3 min and 3-4 hours p.i. to calculate bone uptake after correction for both urinary excretion and soft tissue retention. Results: Bone uptake values of various age-related subgroups showed no significant differences between men and women (p >0.05 ). Furthermore, no differences could be proven between age-matched subgroups of normals and patients with less than 10 metastatic bone lesions, while patients with wide-spread bone metastases revealed significantly increased uptake values. In both men and women highest bone uptake was obtained (p <0.05 ) in subjects younger than 20 years with active epiphyseal growth plates. In men, bone uptake slowly decreased with age up to 60 years and then showed a tendency towards increasing uptake values. In women, the mean uptake reached a minimun in the decade 20-29 years and then slowly increased with a positive linear correlation of age and uptake in subjects older than 55 years (r = 0.57). Conclusion: Since the results proposed in this study are in good agreement with data from literature, the new method used for quantification could be validated in a large number of patients. Furthermore, age- and sexrelated normal bone uptake values of Tc-99m-HDP covering a wide range of age could be presented for this method as a basis for further studies on bone uptake.

Aromatic Volatile Composition of Celery and Celeriac Cultivars

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 556-559 ◽  
Author(s):  
Fredy Van Wassenhove ◽  
Patrick Dirinck ◽  
Georges Vulsteke ◽  
Niceas Schamp
Keyword(s):  
Volatile Compounds ◽  
Chromatographic Method ◽  
Apium Graveolens ◽  
Volatile Components ◽  
Two Dimensional ◽  
Qualitative Composition ◽  
Volatile Composition ◽  
Wide Range ◽  
Gas Chromatographic Method ◽  
Identification And Quantification

A two-dimensional capillary gas chromatographic method was developed to separate and quantify aromatic volatiles of celery in one analysis. The isolation, identification, and quantification of the volatile compounds of four cultivars of blanching celery (Apium graveolens L. var. dulce) and six cultivars of celeriac (Apium graveolens L. var. rapaceum) are described. The qualitative composition of Likens-Nickerson extracts of both cultivars is similar. The concentration of terpenes and phthalides, the key volatile components, found in various cultivars of both celery and celeriac varied over a wide range.

scholarly journals Prediction of Wide Range Two-Dimensional Refractivity Using an IDW Interpolation Method from High-Altitude Refractivity Data of Multiple Meteorological Observatories

2021 ◽  
Vol 11 (4) ◽  
pp. 1431
Author(s):  
Sungsik Wang ◽  
Tae Heung Lim ◽  
Kyoungsoo Oh ◽  
Chulhun Seo ◽  
Hosung Choo
Keyword(s):  
High Altitude ◽  
Korean Peninsula ◽  
Interpolation Method ◽  
Atmospheric Condition ◽  
Propagation Path ◽  
Two Dimensional ◽  
Data Set ◽  
Wide Range ◽  
Atmospheric Data ◽  
Terrain Surface

This article proposes a method for the prediction of wide range two-dimensional refractivity for synthetic aperture radar (SAR) applications, using an inverse distance weighted (IDW) interpolation of high-altitude radio refractivity data from multiple meteorological observatories. The radio refractivity is extracted from an atmospheric data set of twenty meteorological observatories around the Korean Peninsula along a given altitude. Then, from the sparse refractive data, the two-dimensional regional radio refractivity of the entire Korean Peninsula is derived using the IDW interpolation, in consideration of the curvature of the Earth. The refractivities of the four seasons in 2019 are derived at the locations of seven meteorological observatories within the Korean Peninsula, using the refractivity data from the other nineteen observatories. The atmospheric refractivities on 15 February 2019 are then evaluated across the entire Korean Peninsula, using the atmospheric data collected from the twenty meteorological observatories. We found that the proposed IDW interpolation has the lowest average, the lowest average root-mean-square error (RMSE) of ∇M (gradient of M), and more continuous results than other methods. To compare the resulting IDW refractivity interpolation for airborne SAR applications, all the propagation path losses across Pohang and Heuksando are obtained using the standard atmospheric condition of ∇M = 118 and the observation-based interpolated atmospheric conditions on 15 February 2019. On the terrain surface ranging from 90 km to 190 km, the average path losses in the standard and derived conditions are 179.7 dB and 182.1 dB, respectively. Finally, based on the air-to-ground scenario in the SAR application, two-dimensional illuminated field intensities on the terrain surface are illustrated.

scholarly journals Therapy-Resistant Atypical Downbeat Nystagmus with Vertigo Confined to Specific Head-Hanging Positions: Mapping to the Gravity Vector on a Multi-Axis Turntable

2021 ◽  
pp. 464-469
Author(s):  
Dominik Péus ◽  
Dominik Straumann ◽  
Alexander Huber ◽  
Christopher J. Bockisch ◽  
Vincent Wettstein
Keyword(s):  
Hair Cells ◽  
Whole Body ◽  
Downbeat Nystagmus ◽  
Two Dimensional ◽  
Body Rotation ◽  
Therapeutic Approaches ◽  
Atypical Case ◽  
Anterior Semicircular Canal ◽  
Head Positions ◽  
Peripheral Origin

Downbeat nystagmus (DBN) observed in head-hanging positions, may be of central or peripheral origin. Central DBN in head-hanging positions is mostly due to a disorder of the vestibulo-cerebellum, whereas peripheral DBN is usually attributed to canalolithiasis of an anterior semicircular canal. Here, we describe an atypical case of a patient who, after head trauma, experienced severe and stereotypic vertigo attacks after being placed in various head-hanging positions. Vertigo lasted 10–15 s and was always associated with a robust DBN. The provocation of transient vertigo and DBN, which both showed no decrease upon repetition of maneuvers, depended on the yaw orientation relative to the trunk and the angle of backward pitch. On a motorized, multi-axis turntable, we identified the two-dimensional Helmholtz coordinates of head positions at which vertigo and DBN occurred (<i>y</i>-axis: horizontal, space-fixed; <i>z</i>-axis: vertical, and head-fixed; <i>x</i>-axis: torsional, head-fixed, and unchanged). This two-dimensional area of DBN-associated head positions did not change when whole-body rotations took different paths (e.g., by forwarding pitch) or were executed with different velocities. Moreover, the intensity of DBN was also independent of whole-body rotation paths and velocities. So far, therapeutic approaches with repeated liberation maneuvers and cranial vibrations were not successful. We speculate that vertigo and DBN in this patient are due to macular damage, possibly an unstable otolithic membrane that, in specific orientations relative to gravity, slips into a position causing paroxysmal stimulation or inhibition of macular hair cells.

scholarly journals Is contact angle a cause or an effect? – A cautionary tale

2020 ◽  
Vol 146 ◽  
pp. 03004
Author(s):  
Douglas Ruth
Keyword(s):  
Contact Angle ◽  
Solid Surface ◽  
Contact Angles ◽  
Two Dimensions ◽  
Experimental Results ◽  
Flow In Porous Media ◽  
Two Dimensional ◽  
Wide Range ◽  
Fluid Drop ◽  
Surrounding Fluid

The most influential parameter on the behavior of two-component flow in porous media is “wettability”. When wettability is being characterized, the most frequently used parameter is the “contact angle”. When a fluid-drop is placed on a solid surface, in the presence of a second, surrounding fluid, the fluid-fluid surface contacts the solid-surface at an angle that is typically measured through the fluid-drop. If this angle is less than 90°, the fluid in the drop is said to “wet” the surface. If this angle is greater than 90°, the surrounding fluid is said to “wet” the surface. This definition is universally accepted and appears to be scientifically justifiable, at least for a static situation where the solid surface is horizontal. Recently, this concept has been extended to characterize wettability in non-static situations using high-resolution, two-dimensional digital images of multi-component systems. Using simple thought experiments and published experimental results, many of them decades old, it will be demonstrated that contact angles are not primary parameters – their values depend on many other parameters. Using these arguments, it will be demonstrated that contact angles are not the cause of wettability behavior but the effect of wettability behavior and other parameters. The result of this is that the contact angle cannot be used as a primary indicator of wettability except in very restricted situations. Furthermore, it will be demonstrated that even for the simple case of a capillary interface in a vertical tube, attempting to use simply a two-dimensional image to determine the contact angle can result in a wide range of measured values. This observation is consistent with some published experimental results. It follows that contact angles measured in two-dimensions cannot be trusted to provide accurate values and these values should not be used to characterize the wettability of the system.

scholarly journals On local and integrated stress-tensor commutators

2021 ◽  
Vol 2021 (7) ◽  
Author(s):  
Mert Besken ◽  
Jan de Boer ◽  
Grégoire Mathys
Keyword(s):  
Stress Tensor ◽  
Free Field ◽  
Virasoro Algebra ◽  
Light Sheet ◽  
Operator Product ◽  
Local Form ◽  
Two Dimensional ◽  
Conformal Embedding ◽  
General Aspects ◽  
The Right

Abstract We discuss some general aspects of commutators of local operators in Lorentzian CFTs, which can be obtained from a suitable analytic continuation of the Euclidean operator product expansion (OPE). Commutators only make sense as distributions, and care has to be taken to extract the right distribution from the OPE. We provide explicit computations in two and four-dimensional CFTs, focusing mainly on commutators of components of the stress-tensor. We rederive several familiar results, such as the canonical commutation relations of free field theory, the local form of the Poincaré algebra, and the Virasoro algebra of two-dimensional CFT. We then consider commutators of light-ray operators built from the stress-tensor. Using simplifying features of the light sheet limit in four-dimensional CFT we provide a direct computation of the BMS algebra formed by a specific set of light-ray operators in theories with no light scalar conformal primaries. In four-dimensional CFT we define a new infinite set of light-ray operators constructed from the stress-tensor, which all have well-defined matrix elements. These are a direct generalization of the two-dimensional Virasoro light-ray operators that are obtained from a conformal embedding of Minkowski space in the Lorentzian cylinder. They obey Hermiticity conditions similar to their two-dimensional analogues, and also share the property that a semi-infinite subset annihilates the vacuum.

scholarly journals Robust anomalous metallic states and vestiges of self-duality in two-dimensional granular In-InOx composites

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Xinyang Zhang ◽  
Bar Hen ◽  
Alexander Palevski ◽  
Aharon Kapitulnik
Keyword(s):  
Magnetic Field ◽  
Metallic Phase ◽  
Broadband Noise ◽  
Two Dimensional ◽  
Conventional Theory ◽  
Logarithmic Divergence ◽  
Self Duality ◽  
Low Field ◽  
Wide Range ◽  
Low Temperature Resistance

AbstractMany experiments investigating magnetic-field tuned superconductor-insulator transition (H-SIT) often exhibit low-temperature resistance saturation, which is interpreted as an anomalous metallic phase emerging from a ‘failed superconductor’, thus challenging conventional theory. Here we study a random granular array of indium islands grown on a gateable layer of indium-oxide. By tuning the intergrain couplings, we reveal a wide range of magnetic fields where resistance saturation is observed, under conditions of careful electromagnetic filtering and within a wide range of linear response. Exposure to external broadband noise or microwave radiation is shown to strengthen the tendency of superconductivity, where at low field a global superconducting phase is restored. Increasing magnetic field unveils an ‘avoided H-SIT’ that exhibits granularity-induced logarithmic divergence of the resistance/conductance above/below that transition, pointing to possible vestiges of the original emergent duality observed in a true H-SIT. We conclude that anomalous metallic phase is intimately associated with inherent inhomogeneities, exhibiting robust behavior at attainable temperatures for strongly granular two-dimensional systems.

scholarly journals Energetics of mesoscale cell turbulence in two-dimensional monolayers

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Shao-Zhen Lin ◽  
Wu-Yang Zhang ◽  
Dapeng Bi ◽  
Bo Li ◽  
Xi-Qiao Feng
Keyword(s):  
Kinetic Energy ◽  
Tumor Metastasis ◽  
Cell Types ◽  
Boltzmann Distribution ◽  
Scaling Exponent ◽  
Biological Processes ◽  
Two Dimensional ◽  
Cell Monolayers ◽  
Wide Range ◽  
Epithelial Cell Monolayers

AbstractInvestigation of energy mechanisms at the collective cell scale is a challenge for understanding various biological processes, such as embryonic development and tumor metastasis. Here we investigate the energetics of self-sustained mesoscale turbulence in confluent two-dimensional (2D) cell monolayers. We find that the kinetic energy and enstrophy of collective cell flows in both epithelial and non-epithelial cell monolayers collapse to a family of probability density functions, which follow the q-Gaussian distribution rather than the Maxwell–Boltzmann distribution. The enstrophy scales linearly with the kinetic energy as the monolayer matures. The energy spectra exhibit a power-decaying law at large wavenumbers, with a scaling exponent markedly different from that in the classical 2D Kolmogorov–Kraichnan turbulence. These energetic features are demonstrated to be common for all cell types on various substrates with a wide range of stiffness. This study provides unique clues to understand active natures of cell population and tissues.

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