scholarly journals Energetics of mesoscale cell turbulence in two-dimensional monolayers

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Shao-Zhen Lin ◽  
Wu-Yang Zhang ◽  
Dapeng Bi ◽  
Bo Li ◽  
Xi-Qiao Feng
Keyword(s):  
Kinetic Energy ◽  
Tumor Metastasis ◽  
Cell Types ◽  
Boltzmann Distribution ◽  
Scaling Exponent ◽  
Biological Processes ◽  
Two Dimensional ◽  
Cell Monolayers ◽  
Wide Range ◽  
Epithelial Cell Monolayers

AbstractInvestigation of energy mechanisms at the collective cell scale is a challenge for understanding various biological processes, such as embryonic development and tumor metastasis. Here we investigate the energetics of self-sustained mesoscale turbulence in confluent two-dimensional (2D) cell monolayers. We find that the kinetic energy and enstrophy of collective cell flows in both epithelial and non-epithelial cell monolayers collapse to a family of probability density functions, which follow the q-Gaussian distribution rather than the Maxwell–Boltzmann distribution. The enstrophy scales linearly with the kinetic energy as the monolayer matures. The energy spectra exhibit a power-decaying law at large wavenumbers, with a scaling exponent markedly different from that in the classical 2D Kolmogorov–Kraichnan turbulence. These energetic features are demonstrated to be common for all cell types on various substrates with a wide range of stiffness. This study provides unique clues to understand active natures of cell population and tissues.

Charney isotropy and equipartition in quasi-geostrophic turbulence

2010 ◽  
Vol 656 ◽  
pp. 448-457 ◽  
Author(s):  
ANDREAS VALLGREN ◽  
ERIK LINDBORG
Keyword(s):  
High Resolution ◽  
Kinetic Energy ◽  
Horizontal Velocity ◽  
Energy Cascade ◽  
Two Dimensional ◽  
Inverse Energy Cascade ◽  
Wide Range ◽  
Geostrophic Turbulence ◽  
Decaying Turbulence ◽  
Enstrophy Cascade

High-resolution simulations of forced quasi-geostrophic (QG) turbulence reveal that Charney isotropy develops under a wide range of conditions, and constitutes a preferred state also in β-plane and freely decaying turbulence. There is a clear analogy between two-dimensional and QG turbulence, with a direct enstrophy cascade that is governed by the prediction of Kraichnan (J. Fluid Mech., vol. 47, 1971, p. 525) and an inverse energy cascade following the classic k−5/3 scaling. Furthermore, we find that Charney's prediction of equipartition between the potential and kinetic energy in each of the two horizontal velocity components is approximately fulfilled in the inertial ranges.

scholarly journals Culture and differentiation of rabbit intestinal organoids and organoid-derived cell monolayers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Egi Kardia ◽  
Michael Frese ◽  
Elena Smertina ◽  
Tanja Strive ◽  
Xi-Lei Zeng ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Cell Types ◽  
Productive Infection ◽  
Biological Processes ◽  
Cell Monolayers ◽  
Host Interactions ◽  
Monolayer Cultures ◽  
Intestinal Organoids ◽  
Small Intestinal ◽  
Conventional Cell

AbstractOrganoids emulate many aspects of their parental tissue and are therefore used to study pathogen-host interactions and other complex biological processes. Here, we report a robust protocol for the isolation, maintenance and differentiation of rabbit small intestinal organoids and organoid-derived cell monolayers. Our rabbit intestinal spheroid and monolayer cultures grew most efficiently in L-WRN-conditioned medium that contained Wnt, R-spondin and Noggin, and that had been supplemented with ROCK and TGF-β inhibitors. Organoid and monolayer differentiation was initiated by reducing the concentration of the L-WRN-conditioned medium and by adding ROCK and Notch signalling inhibitors. Immunofluorescence staining and RT-qPCR demonstrated that our organoids contained enterocytes, enteroendocrine cells, goblet cells and Paneth cells. Finally, we infected rabbit organoids with Rabbit calicivirus Australia-1, an enterotropic lagovirus that—like many other caliciviruses—does not grow in conventional cell culture. Despite testing various conditions for inoculation, we did not detect any evidence of virus replication, suggesting either that our organoids do not contain suitable host cell types or that additional co-factors are required for a productive infection of rabbit organoids with Rabbit calicivirus Australia-1.

scholarly journals Measurements of transepithelial electrical resistance (TEER) are affected by junctional length in immature epithelial monolayers

2021 ◽  
Author(s):  
Kannapin Felix ◽  
Schmitz Tobias ◽  
Hansmann Jan ◽  
Schlegel Nicolas ◽  
Meir Michael
Keyword(s):  
Epithelial Cell ◽  
Electrical Resistance ◽  
Fluorescein Isothiocyanate ◽  
Cell Types ◽  
Transepithelial Electrical Resistance ◽  
Cell Junctions ◽  
Junctional Complex ◽  
Additional Increase ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers

AbstractThe measurement of transepithelial electrical resistance (TEER) is a common technique to determine the barrier integrity of epithelial cell monolayers. However, it is remarkable that absolute TEER values of similar cell types cultured under comparable conditions show an immense heterogeneity. Based on previous observations, we hypothesized that the heterogeneity of absolute TEER measurements can not only be explained by maturation of junctional proteins but rather by dynamics in the absolute length of cell junctions within monolayers. Therefore, we analyzed TEER in epithelial cell monolayers of Caco2 cells during their differentiation, with special emphasis on both changes in the junctional complex and overall cell morphology within monolayers. We found that in epithelial Caco2 monolayers TEER increased until confluency, then decreased for some time, which was then followed by an additional increase during junctional differentiation. In contrast, permeability of macromolecules measured at different time points as 4 kDA fluorescein isothiocyanate (FITC)-dextran flux across monolayers steadily decreased during this time. Detailed analysis suggested that this observation could be explained by alterations of junctional length along the cell borders within monolayers during differentiation. In conclusion, these observations confirmed that changes in cell numbers and consecutive increase of junctional length have a critical impact on TEER values, especially at stages of early confluency when junctions are immature.

scholarly journals Neighbor-enhanced diffusivity in dense, cohesive cell populations

2021 ◽  
Vol 17 (9) ◽  
pp. e1009447
Author(s):  
Hyun Gyu Lee ◽  
Kyoung J. Lee
Keyword(s):  
Tumor Metastasis ◽  
Cellular Tissue ◽  
Biological Processes ◽  
Two Dimensional ◽  
Cellular Potts Model ◽  
Collective Dynamic ◽  
Tuning Parameters ◽  
Periodic Rotation ◽  
Two Factors ◽  
Cell Cell

The dispersal or mixing of cells within cellular tissue is a crucial property for diverse biological processes, ranging from morphogenesis, immune action, to tumor metastasis. With the phenomenon of ‘contact inhibition of locomotion,’ it is puzzling how cells achieve such processes within a densely packed cohesive population. Here we demonstrate that a proper degree of cell-cell adhesiveness can, intriguingly, enhance the super-diffusive nature of individual cells. We systematically characterize the migration trajectories of crawling MDA-MB-231 cell lines, while they are in several different clustering modes, including freely crawling singles, cohesive doublets of two cells, quadruplets, and confluent population on two-dimensional substrate. Following data analysis and computer simulation of a simple cellular Potts model, which faithfully recapitulated all key experimental observations such as enhanced diffusivity as well as periodic rotation of cell-doublets and cell-quadruplets with mixing events, we found that proper combination of active self-propelling force and cell-cell adhesion is sufficient for generating the observed phenomena. Additionally, we found that tuning parameters for these two factors covers a variety of different collective dynamic states.

The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide

1995 ◽  
Vol 268 (5) ◽  
pp. L699-L722 ◽  
Author(s):  
W. A. Pryor ◽  
G. L. Squadrito
Keyword(s):  
Nitric Oxide ◽  
Hydroxyl Radical ◽  
Biological Effects ◽  
Cell Types ◽  
Boltzmann Distribution ◽  
Acidity Constant ◽  
Peroxynitrous Acid ◽  
Oxidation Reactions ◽  
Solvent Cage ◽  
Wide Range

Nitric oxide and superoxide, which are produced by several cell types, rapidly combine to form peroxynitrite. This reaction can result in nitric oxide scavenging, and thus mitigation of the biological effects of superoxide. Also, superoxide can trap and hence modulate the effects of nitric oxide; superoxide dismutase, by controlling superoxide levels, therefore can influence the reaction pathways open to nitric oxide. The production of peroxynitrite, however, causes its own sequelae of events: Although neither .NO nor superoxide is a strong oxidant, peroxynitrite is a potent and versatile oxidant that can attack a wide range of biological targets. The peroxynitrite anion is relatively stable, but its acid, peroxynitrous acid (HOONO), rearranges to form nitrate with a half-life of approximately 1 s at pH 7, 37 degrees C. HOONO exists as a Boltzmann distribution of rotamers; at 5-37 degrees C HOONO has an apparent acidity constant, pKa,app, of 6.8. Oxidation reactions of HOONO can involve two-electron processes (such as an SN2 displacement) or a one-electron transfer (ET) reaction in which the substrate is oxidized by one electron and peroxynitrite is reduced. These oxidation reactions could involve one of two mechanisms. The first mechanism is homolysis of HOONO to give HO. and .NO2, which initially are held together in a solvent cage. This caged pair of radicals (the "geminate" pair) can either diffuse apart, giving free radicals that can perform oxidations, or react together either to form nitrate or to reform HOONO (a process called cage return). A large amount of cage return can explain the small entropy of activation (Arrhenius A-factor) observed for the decomposition of HOONO. A cage mechanism also can explain the residual yield of nitrate that appears to be formed even in the presence of high concentrations of all of the scavengers studied to date, since scavengers capture only free HO. and .NO2 and not caged radicals. If the cage mechanism is correct, the rate of disappearance of peroxynitrite be slower in solvents of higher viscosity, and we do not find this to be the case. The second mechanism is that an activated isomer of peroxynitrous acid, HOONO*, can be formed in a steady state. The HOONO* mechanism can explain the inability of hydroxyl radical scavengers to completely block either nitrate formation or the oxidation of substrates such as methionine, since HOONO* would be less reactive, and therefore more selective, than the hydroxyl radical itself.(ABSTRACT TRUNCATED AT 400 WORDS)

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Aromatic Volatile Composition of Celery and Celeriac Cultivars

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 556-559 ◽  
Author(s):  
Fredy Van Wassenhove ◽  
Patrick Dirinck ◽  
Georges Vulsteke ◽  
Niceas Schamp
Keyword(s):  
Volatile Compounds ◽  
Chromatographic Method ◽  
Apium Graveolens ◽  
Volatile Components ◽  
Two Dimensional ◽  
Qualitative Composition ◽  
Volatile Composition ◽  
Wide Range ◽  
Gas Chromatographic Method ◽  
Identification And Quantification

A two-dimensional capillary gas chromatographic method was developed to separate and quantify aromatic volatiles of celery in one analysis. The isolation, identification, and quantification of the volatile compounds of four cultivars of blanching celery (Apium graveolens L. var. dulce) and six cultivars of celeriac (Apium graveolens L. var. rapaceum) are described. The qualitative composition of Likens-Nickerson extracts of both cultivars is similar. The concentration of terpenes and phthalides, the key volatile components, found in various cultivars of both celery and celeriac varied over a wide range.

The Departure from Equilibrium of Turbulent Boundary Layers

1968 ◽  
Vol 19 (1) ◽  
pp. 1-19 ◽  
Author(s):  
H. McDonald
Keyword(s):  
Boundary Layer ◽  
Shear Stress ◽  
Kinetic Energy ◽  
Stress Distribution ◽  
Boundary Layers ◽  
Turbulent Boundary Layers ◽  
Two Dimensional ◽  
Pressure Distributions ◽  
Momentum Integral ◽  
State Examination

SummaryRecently two authors, Nash and Goldberg, have suggested, intuitively, that the rate at which the shear stress distribution in an incompressible, two-dimensional, turbulent boundary layer would return to its equilibrium value is directly proportional to the extent of the departure from the equilibrium state. Examination of the behaviour of the integral properties of the boundary layer supports this hypothesis. In the present paper a relationship similar to the suggestion of Nash and Goldberg is derived from the local balance of the kinetic energy of the turbulence. Coupling this simple derived relationship to the boundary layer momentum and moment-of-momentum integral equations results in quite accurate predictions of the behaviour of non-equilibrium turbulent boundary layers in arbitrary adverse (given) pressure distributions.

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