scholarly journals ELICE16INDURES ®: a plant immune-priming activator targeting jasmonate metabolism via TIFY and RHOMBOID proteins in Hordeum vulgare

2021 ◽  
Author(s):  
Geza Hegedus ◽  
Marta Kiniczky ◽  
Agnes Nagy ◽  
Peter Pekker ◽  
Balazs Lang ◽  
...  

Priming activity of plant-based allelochemicals is advanced research nowadays meaning a high potential in sustainable agriculture. The ELICE16INDURES® (RIMPH LTD, Hungary) plant conditioner of CO2 botanical extracts is rich in plant-active ingredients such as phenolic compounds, alkaloids, and flavonoids formulated in small multilamellar liposomes. This product was investigated in autumn barley (Hordeum vulgare). Field experiments of ELICE16INDURES showed augmented NDVI values interconnected with higher photosynthetic activity and yield increase. Background of the better vitality of plants was investigated by whole genomic gene expression profiling and showed an enhanced response to wounding, jasmonic acid, oxidative detoxification, and chloroplast activity. Among top 50 differentially expressed genes the TIFY domain protein TIFY11B and RHOMBOID-like protein 2 related to JA signaling were up-regulated in field-collected samples. Phytotron experiments of barley were set up to validate and evaluate the transcriptomic effect of ELICE16INDURES. Well-studied priming active agents such as salicylic acid and beta-aminobutyric acid were compared with ELICE16INDURES and confirmed as priming inducer material with positive regulation of TIFY11B, TIFY3B, TIFY9, TIF10A, and RHOMBOID like protein 2 by using NGS GEx and RT-qPCR methods.

2014 ◽  
Vol 10 (1) ◽  
pp. 1-15
Author(s):  
Z. Láng

The possible effect of shaker harvest on root damage of 10-year-old cherry trees was studied on a simple tree structure model. The model was composed of elastic trunk and rigid main roots, the ends of which were connected to the surrounding soil via springs and dumping elements. Equations were set up to be able to calculate the relation between shaking height on the trunk and strain in the roots. To get the data for root break and their elongation at different shaking heights on the trunk, laboratory and field experiments were carried out on cherry trees and on their roots. Having evaluated the measured and calculated data it could be concluded that root damage is to be expected even at 3.6% strain and the risk of it increases with increased trunk amplitudes, i.e.with the decrease of shaking heightat smaller stem diameters (i.e. in younger plantation), andif the unbalanced mass of the shaker machine is too large for the given tree size.


2011 ◽  
Vol 48 (No. 1) ◽  
pp. 20-26
Author(s):  
M. Birkás ◽  
T. Szalai ◽  
C. Gyuricza ◽  
M. Gecse ◽  
K. Bordás

This research was instigated by the fact that during the last decade annually repeated shallow disk tillage on the same field became frequent practice in Hungary. In order to study the changes of soil condition associated with disk tillage and to assess it is consequences, long-term tillage field experiments with different levels of nutrients were set up in 1991 (A) and in 1994 (B) on Chromic Luvisol at Gödöllö. The effects of disk tillage (D) and disk tillage combined with loosening (LD) on soil condition, on yield of maize and winter wheat, and on weed infestation were examined. The evaluation of soil condition measured by cone index and bulk density indicated that use of disking annually resulted in a dense soil layer below the disking depth (diskpan-compaction). It was found, that soil condition deteriorated by diskpan-compaction decreased the yield of maize significantly by 20 and 42% (w/w), and that of wheat by 13 and 15% (w/w) when compared to soils with no diskpan-compaction. Averaged over seven years, and three fertilizer levels, the cover % of the total, grass and perennial weeds on loosened soils were 73, 69 and 65% of soils contained diskpan-compaction.


2019 ◽  
Vol 22 (8) ◽  
pp. 939-950 ◽  
Author(s):  
P. N. Mal’chikov ◽  
M. A. Rozova ◽  
A. I. Morgunov ◽  
M. G. Myasnikova ◽  
Yu. I. Zelensky

Identifcation of adaptive responses of breeding material, developed in different breeding centers, helps to purposefully correct these traits where it is necessary. Thus, 42 modern breeding lines from eight institutions of Russia and Kazakhstan were studied in comparison with the historical standard Bezenchukskaya 139 in trails of 16-17 and 18 KASIB-SDW (Kazakhstan-Siberian net for wheat improvement, spring durum wheat) in 2015–2017. Field experiments and yield measurements in each ecological cite were similar. To solve these tasks of the experiment, two-factor ANOVA, methods for adaptability assessment cluster analysis and principle component method were applied. As a result, it was established that 1) genotype and genotype – environment interaction (overall 15.8–23.5 % of total dispersion) had signifcant effect on yield variability; 2) genotype – environment interactions were of linear nature and had no destabilizing effect; 3) all the genotypes tested can be distributed in three clusters, the frst one for locally adapted varieties, the third for varieties of a wide areal, the second included genotypes with intermediate characteristics; 4) breeding centers of the Federal Altai Scientifc Centre of Agro-Biotechnologies and of the Research Institute of Agriculture of South-East produced predominantly varieties of local importance, the Samara Research Institute of Agriculture – varieties of wide area; 5) a stable trend of increased mean yield compared to historical standard Bezenchukskaya 139 over ecological sites was observed only for Samara varieties; 6) varieties of all the breeding centers had no stable difference from Bezenchukskaya 139 concerning stability and responsiveness, which can be explained by an incomplete breeding process for these parameters and confrms the importance of the KASIB program; 7) a trend of yield increase compared to Bezenchukskaya 139 under testing in defnite local environments had more stable parameters with the largest progress observed in the Altai Research Institute of Agriculture (135.4 and 163.2 % to Bezenchukskaya 139), which can be explained by a high efciency of breeding of locally adapted varieties in the breeding center.


2020 ◽  
Author(s):  
D Steinke ◽  
TWA Braukmann ◽  
L Manerus ◽  
A Woodhouse ◽  
V Elbrecht

AbstractThe Malaise trap has gained popularity for assessing diverse terrestrial arthropod communities because it collects large samples with modest effort. A number of factors that influence collection efficiency, placement being one of them. For instance, when designing larger biotic surveys using arrays of Malaise traps we need to know the optimal distance between individual traps that maximises observable species richness and community composition. We examined the influence of spacing between Malaise traps by metabarcoding samples from two field experiments at a site in Waterloo, Ontario, Canada. For one experiment, we used two trap pairs deployed at weekly increasing distance (3m increments from 3 to 27 m). The second experiment involved a total of 10 traps set up in a row at 3m distance intervals for three consecutive weeks.Results show that community similarity of samples decreases over distance between traps. The amount of species shared between trap pairs shows drops considerably at about 15m trap-to-trap distance. This change can be observed across all major taxonomic groups and for two different habitat types (grassland and forest). Large numbers of OTUs found only once within samples cause rather large dissimilarity between distance pairs even at close proximity. This could be caused by a large number of transient species from adjacent habitat which arrive at the trap through passive transport, as well as capture of rare taxa, which end up in different traps by chance.


2015 ◽  
Vol 95 (8) ◽  
pp. 1607-1612 ◽  
Author(s):  
E.S. Mekhova ◽  
P.Y. Dgebuadze ◽  
V.N. Mikheev ◽  
T.A. Britayev

Previous experiments with the comatulid Himerometra robustipinna (Carpenter, 1881) demonstrated intensive host-to-host migration processes for almost all symbiotic species both within host aggregations and among hosts separated by several metres. The aim of this study was to check the ability of symbionts to complete long-distance migrations, by means of two in situ experiments which depopulated the crinoid host. Two different sets of field experiments were set up: exposure of depopulated crinoids (set 1) on stony ‘islands’ isolated from native crinoid assemblages by sandy substrate, and (set 2) in cages suspended in the water column. Hosts from set 1 were exposed for 1, 2, 3 and 4 weeks to assess whether substrate has an influence on the symbionts' long-distance migrations. In set 2 cages were exposed for 10–11 days, aiming to check whether symbionts were able to disperse through the water column with currents. These experiments allow the conclusion that post-settled symbionts can actively migrate among their hosts. Symbionts are able to reach their hosts by employing two different ‘transport corridors’, by drifting or swimming in water column, and by moving on the bottom. Comparison of experimental results allows the division of symbionts into two conventional groups according to the dispersal ability of their post-settled stages: (1) species able to complete long-distance migrations, (2) species unable to migrate or having limited dispersal ability. The finding of the free-living shrimp Periclimenes diversipes Kemp, 1922 in set 2 raises the question about the factors that affect such a high degree of specialization of crinoid assemblages.


2013 ◽  
Vol 153 (1) ◽  
pp. 42-55 ◽  
Author(s):  
C. A. WHITE ◽  
S. E. ROQUES ◽  
P. M. BERRY

SUMMARYThe aim of the present study was to evaluate the effects on yield, oil concentration and nitrogen (N) uptake efficiency of N fertilizer applied to the foliage of oilseed rape during and soon after flowering. Four field experiments were conducted in the UK during the 2008/09 and 2009/10 seasons which investigated six rates of soil-applied N (ammonium nitrate) ranging from 0 to 280 or 320 kg N/ha with each treatment followed by 0 or 40 kg/ha of foliar N applied as a solution of urea at the end of flowering. Each experiment also investigated five rates of foliar N ranging from 0 to 120 kg N/ha applied at the end of flowering and five timings of foliar N (40 kg N/ha) from mid-flowering to 2 weeks after the end of flowering.Foliar N at 40 kg N/ha applied at the end of flowering significantly increased the seed yield in three of the four experiments. The seed yield increase across all four experiments was 0·25 t/ha (range of 0–0·41 t/ha). In two experiments, the increase in seed yield in response to foliar N occurred irrespective of whether it followed sub-optimal or super-optimal rates of soil-applied N; in one experiment there was a greater response at sub-optimal soil-applied N rates. The foliar N treatment reduced the seed oil concentration by 11 g/kg and increased seed protein concentration by 11 g/kg. Similar yield responses were observed for foliar N applications between mid-flowering and 2 weeks after the end of flowering. The efficiency with which foliar N was taken up into the plant varied between 0 and 100% with an average uptake efficiency across the four experiments of 61%.


1986 ◽  
Vol 66 (3) ◽  
pp. 795-799 ◽  
Author(s):  
LUC COUTURE ◽  
DANIEL ISFAN

The effect of nitrogen fertilizers applied as urea or ammonium nitrate at doses of 0, 40, 80, 120, and 160 kg ha−1 on scald in spring barley (Hordeum vulgare) grown on two different soils was examined during 2 yr. A strong significant positive correlation was found between nitrogen dose and severity of scald during the 2 yr. Nitrogen influenced disease severity the same way in both soils although the absolute level of disease was higher in plots set up in sandy loam than in clay soil. Disease severity was approximately the same in barley fertilized with urea or ammonium nitrate.Key words: Nitrogen fertilizer, nitrogen form, barley, Hordeum vulgare, scald, Rhynchosporium secalis


2018 ◽  
Vol 98 (6) ◽  
pp. 1304-1320
Author(s):  
L.A. Thompson ◽  
S.M. Strydhorst ◽  
L.M. Hall ◽  
R.C. Yang ◽  
D. Pauly ◽  
...  

The area sown to barley (Hordeum vulgare L.) in Alberta, Canada, and the rate of yield increase relative to other major crops have declined in recent decades. Advanced agronomic management of feed barley may increase the seeded area and differentially influence cultivar performance. Field experiments were conducted from 2014 to 2016 at 11 rainfed and three irrigated environments in Alberta to evaluate the performance of 10 feed barley cultivars under standard and advanced agronomic management. Advanced management included supplemental postemergence N, the plant growth regulator chlormequat chloride, and two foliar fungicide applications. Cultivars responded similarly to management in the low disease pressure environments encountered in the study. The two-row cultivars CDC Austenson, Xena, and CDC Coalition were the highest yielding overall, while Champion had the greatest yields in moisture-limited environments. The feed grain quality of two-row cultivars was superior to six-row cultivars. Recently released six-row cultivars were among the lowest yielding. Negative or static yield increases were observed for all newer cultivars (2006–2013 registrations) compared with the older cultivar, Xena (2000 registration). In comparison, the overall 9.3% yield increase from advanced management was notable. Advanced management yield increases were greater (8%–18%) in environments with 251–502 mm of precipitation and smaller (1%–3%) in moisture-limited environments. Management had negligible effects on lodging and grain quality. Optimal yield and quality were achieved with the two-row cultivars, CDC Austenson, Xena, or CDC Coalition, and advanced management in high precipitation environments. In environments with less precipitation, optimal yields were achieved with Champion and standard management.


1975 ◽  
Vol 15 (73) ◽  
pp. 276 ◽  
Author(s):  
RG Rees ◽  
GJ Platz

The control of wheat leaf rust (Puccinia recondita tritici) by seed or foliar applications of 4-n-butyl-l,2,4-triazole (RH-124) has been examined in field experiments over three years at Gatton, Queensland. Single foliar sprays of RH-124 at rates as low as 100 g a.i, ha-1 have given excellent control of leaf rust. One such spray resulted in a yield increase of 30 per cent. A spray at 400 g a.i. ha-1 increased yield by 46 per cent compared with an increase of 61 per cent in the rust-free reference. Applications around the early boot stage of crop development were the most effective. Treatment of seed with RH-124 resulted in some control of leaf rust in one season with an associated yield increase of 22 per cent. Although RH-124 appears promising, additional studies are required before use of the fungicide on commercial wheat crops would be accepted.


1992 ◽  
Vol 32 (4) ◽  
pp. 465 ◽  
Author(s):  
AD Doyle ◽  
RW Kingston

The effect of sowing rate (10-110 kg/ha) on the grain yield of barley (Hordeum vulgare L.) was determined from a total of 20 field experiments conducted in northern New South Wales from 1983 to 1986. Effects of sowing rate on kernel weight and grain protein percentage were also determined from 12 experiments conducted in 1985 and 1986. Two barley varieties were tested each year. In all years fallow plus winter rainfall was equal to or greater than average. Grain yield increased with higher sowing rates in most experiments, with the response curve reaching a plateau above 60-70 kg/ha. For 13 of the 40 variety x year combinations, grain yield fell at the highest sowing rates. Only in an experiment where lodging increased substantially with higher sowing rates was there a reduction in yield at a sowing rate of 60 kg/ha. The average sowing rate for which 5 kg grain was produced per kg of seed sown was 63 kg/ha. Grain protein percentage usually fell, and kernel weight invariably fell, with increasing sowing rate. Increasing sowing rates from the normal commercial rate of 35 kg/ha to a rate of 60 kg/ha typically increased grain yields by 100-400 kg/ha, decreased kernel weight by 0.4-2.0 mg, and decreased grain protein by up to 0.5 percentage points. In no case was the grain weight reduced to below malting specifications. It was concluded that sowing rates for barley in northern New South Wales should be increased to about 60 kg/ha.


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