scholarly journals Cost-effective, high-throughput phenotyping system for 3D reconstruction of fruit form

2021 ◽  
Author(s):  
Mitchell J Feldmann ◽  
Amy Tabb
Keyword(s):  
3D Imaging ◽  
Low Cost ◽  
Ground Truth ◽  
Cost Effective ◽  
Fruit Shape ◽  
3D Analysis ◽  
Image Capture ◽  
High Throughput Phenotyping ◽  
3D Printed ◽  
Specialized Hardware

Reliable phenotyping methods that are simple to operate and inexpensive to deploy are critical for studying quantitative traits in plants. Traditional fruit shape phenotyping relies on human raters or 2D analyses to assess form, e.g., size and shape. Systems for 3D imaging using multi-view stereo have been implemented, but frequently rely on commercial software and/or specialized hardware, which can lead to limitations in accessibility and scalability. We present a complete system constructed of consumer-grade components for capturing, calibrating, and reconstructing the 3D form of small-to-moderate sized fruits and tubers. Data acquisition and image capture sessions are 9 seconds to capture 60 images. The initial prototype cost was $1600 USD. We measured accuracy by comparing reconstructed models of 3D printed ground truth objects to the original digital files of those same ground truth objects. The R2 between length of the primary, secondary, and tertiary axes, volume, and surface area of the ground-truth object and the reconstructed models was > 0.97 and root-mean square error (RMSE) was <3mm for objects without locally concave regions. Measurements from 1mm and 2mm resolution reconstructions were consistent (R2 > 0.99). Qualitative assessments were performed on 48 fruit and tubers, including 18 strawberries, 12 potatoes, 5 grapes, 7 peppers, and 4 Bosch and 2 red Anjou pears. Our proposed phenotyping system is fast, relatively low cost, and has demonstrated accuracy for certain shape classes, and could be used for the 3D analysis of fruit form.

Droplet generation in cross-flow for cost-effective 3D-printed “plug-and-play” microfluidic devices

RSC Advances ◽  
2016 ◽  
Vol 6 (84) ◽  
pp. 81120-81129 ◽  
Author(s):  
Jia Ming Zhang ◽  
Andres A. Aguirre-Pablo ◽  
Er Qiang Li ◽  
Ulrich Buttner ◽  
Sigurdur T. Thoroddsen
Keyword(s):  
Low Cost ◽  
Cross Flow ◽  
Microfluidic Devices ◽  
Cost Effective ◽  
Droplet Generation ◽  
Channel Design ◽  
Plug And Play ◽  
3D Printed

Novel low-cost 3D-printed plug-and-play microfluidic devices have been developed for droplet generation and applications. By combining a commercial tubing with the printed channel design we can generate well-controlled droplets down to 50 μm.

scholarly journals Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork

2020 ◽  
Author(s):  
Matthew Wincott ◽  
Andrew Jefferson ◽  
Ian M. Dobbie ◽  
Martin J. Booth ◽  
Ilan Davis ◽  
...  
Keyword(s):  
Open Source ◽  
Fluorescence Imaging ◽  
Imaging System ◽  
Low Cost ◽  
Dark Field ◽  
List Type ◽  
Image Capture ◽  
Fluorescence Imaging System ◽  
3D Printed ◽  
Open Source Hardware

ABSTRACTCommercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens.HighlightsPortable, low cost, self-build from 3D printed and commodity componentsMultimodal imaging: bright field, dark field, pseudo-phase and fluorescenceAutomated XYZt imaging from a tablet or smartphone via a simple GUIWide ranging applications in teaching, outreach and fieldworkOpen source hardware and software design, allowing user modification

scholarly journals 3D Printing of Rapid, Low-Cost and Patient-Specific Models of Brain Vasculature for Use in Preoperative Planning in Clipping of Intracranial Aneurysms

2021 ◽  
Vol 10 (6) ◽  
pp. 1201
Author(s):  
Maciej Błaszczyk ◽  
Redwan Jabbar ◽  
Bartosz Szmyd ◽  
Maciej Radek
Keyword(s):  
3D Visualization ◽  
Low Cost ◽  
Cost Benefit ◽  
Image Data ◽  
Cost Effective ◽  
3D Models ◽  
Urgent Care ◽  
Patient Specific ◽  
Stl File ◽  
3D Printed

We developed a practical and cost-effective method of production of a 3D-printed model of the arterial Circle of Willis of patients treated because of an intracranial aneurysm. We present and explain the steps necessary to produce a 3D model from medical image data, and express the significant value such models have in patient-specific pre-operative planning as well as education. A Digital Imaging and Communications in Medicine (DICOM) viewer is used to create 3D visualization from a patient’s Computed Tomography Angiography (CTA) images. After generating the reconstruction, we manually remove the anatomical components that we wish to exclude from the print by utilizing tools provided with the imaging software. We then export this 3D reconstructions file into a Standard Triangulation Language (STL) file which is then run through a “Slicer” software to generate a G-code file for the printer. After the print is complete, the supports created during the printing process are removed manually. The 3D-printed models we created were of good accuracy and scale. The median production time used for the models described in this manuscript was 4.4 h (range: 3.9–4.5 h). Models were evaluated by neurosurgical teams at local hospital for quality and practicality for use in urgent and non-urgent care. We hope we have provided readers adequate insight into the equipment and software they would require to quickly produce their own accurate and cost-effective 3D models from CT angiography images. It has become quite clear to us that the cost-benefit ratio in the production of such a simplified model is worthwhile.

scholarly journals Cost-Effective Active Learning from Diverse Labelers

2017 ◽  
Author(s):  
Sheng-Jun Huang ◽  
Jia-Lve Chen ◽  
Xin Mu ◽  
Zhi-Hua Zhou
Keyword(s):  
Cost Effectiveness ◽  
Active Learning ◽  
Low Cost ◽  
Selection Criterion ◽  
Ground Truth ◽  
Cost Effective ◽  
Classification Model ◽  
Data Sets ◽  
Active Selection ◽  
The Cost

In traditional active learning, there is only one labeler that always returns the ground truth of queried labels. However, in many applications, multiple labelers are available to offer diverse qualities of labeling with different costs. In this paper, we perform active selection on both instances and labelers, aiming to improve the classification model most with the lowest cost. While the cost of a labeler is proportional to its overall labeling quality, we also observe that different labelers usually have diverse expertise, and thus it is likely that labelers with a low overall quality can provide accurate labels on some specific instances. Based on this fact, we propose a novel active selection criterion to evaluate the cost-effectiveness of instance-labeler pairs, which ensures that the selected instance is helpful for improving the classification model, and meanwhile the selected labeler can provide an accurate label for the instance with a relative low cost. Experiments on both UCI and real crowdsourcing data sets demonstrate the superiority of our proposed approach on selecting cost-effective queries.

scholarly journals Custom built scanner and simple image processing pipeline enables low-cost, high-throughput phenotyping of maize ears

2019 ◽  
Author(s):  
Cedar Warman ◽  
John E Fowler
Keyword(s):  
Image Processing ◽  
High Throughput ◽  
Low Cost ◽  
Digital Camera ◽  
Cost Effective ◽  
Source Image ◽  
Processing Pipeline ◽  
Effective Combination ◽  
Transmission Data ◽  
High Throughput Phenotyping

AbstractHigh-throughput phenotyping systems are becoming increasingly powerful, dramatically changing our ability to document, measure, and detect phenomena. Unfortunately, taking advantage of these trends can be difficult for scientists with few resources, particularly when studying nonstandard biological systems. Here, we describe a powerful, cost-effective combination of a custom-built imaging platform and open-source image processing pipeline. Our maize ear scanner was built with off-the-shelf parts for <$80. When combined with a cellphone or digital camera, videos of rotating maize ears were captured and digitally flattened into projections covering the entire surface of the ear. Segregating GFP and anthocyanin seed markers were clearly distinguishable in ear projections, allowing manual annotation using ImageJ. Using this method, statistically powerful transmission data can be collected for hundreds of maize ears, accelerating the phenotyping process.

scholarly journals PSIX-37 Considerations for using game cameras for on-farm behavior of beef cattle

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 322-323
Author(s):  
Benjamin Crites ◽  
Nicholas Roy ◽  
Clay Stamm ◽  
Brandon Sears ◽  
Matthew Campbell ◽  
...  
Keyword(s):  
Low Cost ◽  
Cost Effective ◽  
Battery Life ◽  
Image Capture ◽  
County Extension Agents ◽  
Trail Camera ◽  
Short Video ◽  
Time Utilization ◽  
Trail Cameras ◽  
On Farm

Abstract On-farm studies provide an opportunity to build relationships with clientele while answering applied science questions. Further, game trail cameras are a low-cost technology that may be an alternative to visually observing livestock for behavior data. An on-farm study involving eight county Extension agents and producers investigated game cameras to capture cattle visitation to mineral feeders. Three different models of Browning game trail cameras (Prometheus Group LLC, Birmingham, AL) each with timelapse feature were utilized. A three-page document provided background and methodology for the study. Details for placement of the trail camera were provided to provide consistency across farms. Cameras were pre-programmed to settings for image capture. A video for camera setup was also provided in the event that cameras reset to default settings due to battery failure. Timelapse feature was utilized providing image capture from sunrise to sunset at 2 min intervals. Several observations were noted to improve data capture in the future. Differences existed in battery utilization between camera models with one model only capturing images for approximately 2 of the 7 d periods while others had more than 80% battery life remaining after 7 d. Cameras were to be oriented facing south to avoid sun glare. Sun glare occurred at sunrise on most cameras and was particularly bad when dew was present on the camera lens. Mineral feeders need to be secured in place as livestock may move feeders out of the frame of the images captured. Still photos are insufficient in assessing behavior and short video clips are expected to improve interpretation especially when multiple animals visit the feeder at the same time. Utilization of human observation was not useful unless observation was performed at sunrise. Game trail cameras have the potential to be a cost-effective tool in capturing on-farm data of cattle behavior.

scholarly journals COST EFFECTIVE SPHERICAL PHOTOGRAMMETRY: A NOVEL FRAMEWORK FOR THE SMART MANAGEMENT OF COMPLEX URBAN ENVIRONMENTS

2020 ◽  
Vol XLIII-B4-2020 ◽  
pp. 441-448
Author(s):  
S. Chiappini ◽  
A. Fini ◽  
E. S. Malinverni ◽  
E. Frontoni ◽  
G. Racioppi ◽  
...  
Keyword(s):  
Point Cloud ◽  
Low Cost ◽  
Laser Scanner ◽  
Ground Truth ◽  
Cost Effective ◽  
Data Retrieval ◽  
Acquisition Time ◽  
Cloud Data ◽  
Tax Assessment ◽  
Final Goal

Abstract. The development and urban planning of a modern city, nowadays, should be entrusted on the implementation of methods and techniques which require a management of complex information. The final goal is to support local authorities for the decision making. Finding data that are often heterogeneous but nevertheless connected to each other is useful to create a virtuous management model based on an empirical and objective study system. It will therefore be important to develop a system of data retrieval, analysis and management as accurate as possible, usable by all the actors involved in the governance of the territories. The article focuses on the implementation of an effective workflow for the management of complex urban data, the final goal of such framework, is the creation of a Smart City 3D Platform capable of providing innovative services for tax assessment and collection. In particular, it investigates over the potential of using spherical photogrammetry, to guarantee fast, low-cost and reliable acquisition time. The resulting 3D model has been then georeferenced with GNSS coordinates to ensure the desired precision, while the assessment of the model has been done using laser scanner data as a ground truth. The point cloud obtained from the processing can be managed and edited in a WEBGIS, which merges 2D (cadastral register) and 3D (point cloud) data. The project is the result of the collaboration between the Università Politecnica delle Marche and the Company Andreani Tributi srl, with the aim of collecting information about the advertising structures present in the city of Brescia (Italy) for tax assessment.

scholarly journals Encapsulation of Fiber Optic Sensors in 3D Printed Packages for Use in Civil Engineering Applications: A Preliminary Study

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1689 ◽  
Author(s):  
Richard Scott ◽  
Miodrag Vidakovic ◽  
Sanjay Chikermane ◽  
Brett McKinley ◽  
Tong Sun ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Civil Engineering ◽  
Low Cost ◽  
Cost Effective ◽  
Fiber Optic Sensors ◽  
Engineering Applications ◽  
Considerable Potential ◽  
Challenging Environment ◽  
3D Printed

Fiber optic sensors have considerable potential for measuring strains in the challenging environment posed by today’s civil engineering applications. Their long-term reliability and stability are particularly important attributes for assessing, with confidence, effects such as cracking and response to normal (and abnormal) loads. However, given the fragile nature of the bare fiber, the sensors must be packaged to achieve adequate robustness but the resulting increased cost of installation can frequently limit the number of sensors which can be installed or their use may have to be ruled out altogether due to these financial constraints. There is thus potential for the development of a more affordable type of packaging and this paper describes work undertaken to produce a cost-effective and easy-to-use technique for encapsulating fiber optic sensors in resin, taking advantage of 3D printing techniques which are widely available and at low cost. This approach can be used to produce a robust, inexpensive packaged sensor system which is seen as being suitable to be extended to a wider range of uses including installation in concrete structures prior to casting. To evaluate this approach, several such 3D printed package types and geometries are described and their behavior is assessed from a programme of laboratory trials, the results of which are presented in this paper. This proof-of-concept testing has demonstrated the considerable potential which 3D printed packages have and the scope for further development and consequent use in civil engineering applications. Areas showing promise and potential, which have been identified from the work undertaken, are discussed.

scholarly journals Multifunction control and evaluation of a 3D printed hand prosthesis with the Myo armband by hand amputees

2018 ◽  
Author(s):  
M. Cognolato ◽  
M. Atzori ◽  
C. Marchesin ◽  
S. Marangon ◽  
D. Faccio ◽  
...  
Keyword(s):  
Pediatric Population ◽  
Low Cost ◽  
Cost Effective ◽  
3D Models ◽  
Simple Extension ◽  
Hand Prosthesis ◽  
Prosthetic Hands ◽  
Custom Made ◽  
Myo Armband ◽  
3D Printed

AbstractUpper limb amputations are highly impairing injuries that can substantially limit the quality of life of a person. The most advanced dexterous prosthetic hands have remarkable mechanical features. However, in most cases, the control systems are a simple extension of basic control protocols, making the use of the prosthesis not intuitive and sometimes complex. Furthermore, the cost of dexterous prosthetic hands is often prohibitive, especially for the pediatric population and developing countries. 3D printed hand prostheses can represent an opportunity for the future. Open 3D models are increasingly being released, even for dexterous prostheses that are capable of moving each finger individually and actively rotating the thumb. However, the usage and test of such devices by hand amputees (using electromyography and classification methods) is not well explored. The aim of this article is to investigate the usage of a cost-effective system composed of a 3D printed hand prosthesis and a low-cost myoelectric armband. Two subjects with transradial amputation were asked to wear a custom-made socket supporting the HANDi Hand and the Thalmic Labs Myo armband. Afterwards, the subjects were asked to control and use the prosthetic hand to grasp several objects by attempting to perform a set of different hand gestures. Both the HANDi Hand and the Myo armband performed well during the test, which is encouraging considering that the HANDi Hand was developed as a research platform. The results are promising and show the feasibility of the multifunction control of dexterous 3D printed hand prostheses based on low-cost setups. Factors as the level of the amputation, neuromuscular fatigue and mechanical limitations of the 3D printed hand prosthesis can influence the performance of the setup. Practical aspects such as usability and robustness will need to be addressed for successful application in daily life. A video of the tests can be found at the following link:https://youtu.be/iPSCAbd17Qw

ASSESSMENT OF THE PLATELET COUNT IN THE PREGNANT WOMEN IN IGIMS, PATNA, BIHAR

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Tanwi Singh ◽  
Anshuman Sinha
Keyword(s):  
Platelet Count ◽  
Pregnant Women ◽  
Screening Test ◽  
Low Cost ◽  
Medical Science ◽  
Cost Effective ◽  
Platelet Indices ◽  
Increased Risk ◽  
Low Platelet Count ◽  
Severity Of The Disease

The major risk associated with low platelet count in pregnancy is the increased risk of bleeding during the childbirth or post that. There is an increased blood supply to the uterus during pregnancy and the surgical procedure requires cutting of major blood vessels. Women with thrombocytopenia are at increased risk of losing excessive blood. The risk is more in case of caesarean delivery as compared to vaginal delivery. Hence based on above findings the present study was planned for Assessment of the Platelet Count in the Pregnant Women in IGIMS, Patna, Bihar. The present study was planned in Department of Pathology, Indira Gandhi Institute of Medical Science, Patna, Bihar, India. The present study was planned from duration of January 2019 to June 2019. In the present study 200 pregnant females samples received for the platelet estimation were enrolled in the present study. Clinically platelet indices can be a useful screening test for early identification of preeclampsia and eclampsia. Also platelet indices can assess the prognosis of this disease in pregnant women and can be used as an effective prognostic marker because it correlates with severity of the disease. Platelet count is a simple, low cost, and rapid routine screening test. Hence the data generated from the present study concludes that platelet count can be used as a simple and cost effective tool to monitor the progression of preeclampsia, thereby preventing complications to develop during the gestational period. Keywords: Platelet Count, Pregnant Women, IGIMS, Patna, Bihar, etc.

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