scholarly journals Arginine methylation helps SepIVA balance regulation of septation and elongation in Mycobacterium smegmatis

2021 ◽  
Author(s):  
Angela H Freeman ◽  
Karen Tembiwa ◽  
James R Brenner ◽  
Michael R Chase ◽  
Sarah M Fortune ◽  
...  
Keyword(s):  
Cell Wall ◽  
Mycobacterium Smegmatis ◽  
Physiological Role ◽  
Arginine Methylation ◽  
Post Translational Modification ◽  
Polar Localization ◽  
Division Factor ◽  
Protein Arginine Methylation ◽  
First Time

AbstractGrowth of mycobacterial cells requires successful coordination between elongation and division of the cell wall. However, it is not clear which factors directly mediate this coordination. Here, we studied the function and post-translational modification of an essential division factor, SepIVA, in Mycobacterium smegmatis. We find that SepIVA is arginine methylated, and that these modifications alter both division and polar elongation of Msmeg. Furthermore, SepIVA impacts the localization of MurG. Polar localization of MurG correlates with polar elongation in arginine methylation mutants of sepIVA. These results establish SepIVA as a regulator of both elongation and division, and characterize a physiological role for protein arginine methylation for the first time in bacteria.

Biochemical and Computational Approaches for the Large-Scale Analysis of Protein Arginine Methylation by Mass Spectrometry

2020 ◽  
Vol 21 (7) ◽  
pp. 725-739
Author(s):  
Daniele Musiani ◽  
Enrico Massignani ◽  
Alessandro Cuomo ◽  
Avinash Yadav ◽  
Tiziana Bonaldi
Keyword(s):  
Mass Spectrometry ◽  
Large Scale ◽  
High Resolution Mass Spectrometry ◽  
Arginine Methylation ◽  
Research Field ◽  
Scale Analysis ◽  
Post Translational Modification ◽  
Large Scale Analysis ◽  
Protein Arginine Methylation

: The absence of efficient mass spectrometry-based approaches for the large-scale analysis of protein arginine methylation has hindered the understanding of its biological role, beyond the transcriptional regulation occurring through histone modification. In the last decade, however, several technological advances of both the biochemical methods for methylated polypeptide enrichment and the computational pipelines for MS data analysis have considerably boosted this research field, generating novel insights about the extent and role of this post-translational modification. : Here, we offer an overview of state-of-the-art approaches for the high-confidence identification and accurate quantification of protein arginine methylation by high-resolution mass spectrometry methods, which comprise the development of both biochemical and bioinformatics methods. The further optimization and systematic application of these analytical solutions will lead to ground-breaking discoveries on the role of protein methylation in biological processes.

scholarly journals The Role of Protein Arginine Methylation as Post-Translational Modification on Actin Cytoskeletal Components in Neuronal Structure and Function

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1079
Author(s):  
Britta Qualmann ◽  
Michael M. Kessels
Keyword(s):  
Actin Filament ◽  
Regulatory Mechanism ◽  
De Novo ◽  
Arginine Methylation ◽  
Loss Of Function ◽  
Filament Formation ◽  
Post Translational Modification ◽  
Neuronal Structure ◽  
Actin Nucleation ◽  
Protein Arginine Methylation

The brain encompasses a complex network of neurons with exceptionally elaborated morphologies of their axonal (signal-sending) and dendritic (signal-receiving) parts. De novo actin filament formation is one of the major driving and steering forces for the development and plasticity of the neuronal arbor. Actin filament assembly and dynamics thus require tight temporal and spatial control. Such control is particularly effective at the level of regulating actin nucleation-promoting factors, as these are key components for filament formation. Arginine methylation represents an important post-translational regulatory mechanism that had previously been mainly associated with controlling nuclear processes. We will review and discuss emerging evidence from inhibitor studies and loss-of-function models for protein arginine methyltransferases (PRMTs), both in cells and whole organisms, that unveil that protein arginine methylation mediated by PRMTs represents an important regulatory mechanism in neuritic arbor formation, as well as in dendritic spine induction, maturation and plasticity. Recent results furthermore demonstrated that arginine methylation regulates actin cytosolic cytoskeletal components not only as indirect targets through additional signaling cascades, but can also directly control an actin nucleation-promoting factor shaping neuronal cells—a key process for the formation of neuronal networks in vertebrate brains.

scholarly journals Protein arginine methyltransferases: promising targets for cancer therapy

2021 ◽  
Author(s):  
Jee Won Hwang ◽  
Yena Cho ◽  
Gyu-Un Bae ◽  
Su-Nam Kim ◽  
Yong Kee Kim
Keyword(s):  
Cell Cycle ◽  
Cancer Therapy ◽  
Phase 1 ◽  
Cell Types ◽  
Arginine Methylation ◽  
Protein Methylation ◽  
Post Translational Modification ◽  
Nonhistone Proteins ◽  
Protein Arginine Methyltransferases ◽  
Protein Arginine Methylation

AbstractProtein methylation, a post-translational modification (PTM), is observed in a wide variety of cell types from prokaryotes to eukaryotes. With recent and rapid advancements in epigenetic research, the importance of protein methylation has been highlighted. The methylation of histone proteins that contributes to the epigenetic histone code is not only dynamic but is also finely controlled by histone methyltransferases and demethylases, which are essential for the transcriptional regulation of genes. In addition, many nonhistone proteins are methylated, and these modifications govern a variety of cellular functions, including RNA processing, translation, signal transduction, DNA damage response, and the cell cycle. Recently, the importance of protein arginine methylation, especially in cell cycle regulation and DNA repair processes, has been noted. Since the dysregulation of protein arginine methylation is closely associated with cancer development, protein arginine methyltransferases (PRMTs) have garnered significant interest as novel targets for anticancer drug development. Indeed, several PRMT inhibitors are in phase 1/2 clinical trials. In this review, we discuss the biological functions of PRMTs in cancer and the current development status of PRMT inhibitors in cancer therapy.

scholarly journals Arginine Methylation in Brain Tumors: Tumor Biology and Therapeutic Strategies

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 124
Author(s):  
Jean-Paul Bryant ◽  
John Heiss ◽  
Yeshavanth Kumar Banasavadi-Siddegowda
Keyword(s):  
Brain Tumors ◽  
Cancer Biology ◽  
Metabolic Diseases ◽  
Tumor Biology ◽  
Arginine Methylation ◽  
Post Translational Modification ◽  
Cellular Regulation ◽  
Aberrant Expression ◽  
Arginine Residues ◽  
Protein Arginine Methylation

Protein arginine methylation is a common post-translational modification that plays a pivotal role in cellular regulation. Protein arginine methyltransferases (PRMTs) catalyze the modification of target proteins by adding methyl groups to the guanidino nitrogen atoms of arginine residues. Protein arginine methylation takes part in epigenetic and cellular regulation and has been linked to neurodegenerative diseases, metabolic diseases, and tumor progression. Aberrant expression of PRMTs is associated with the development of brain tumors such as glioblastoma and medulloblastoma. Identifying PRMTs as plausible contributors to tumorigenesis has led to preclinical and clinical investigations of PRMT inhibitors for glioblastoma and medulloblastoma therapy. In this review, we discuss the role of arginine methylation in cancer biology and provide an update on the use of small molecule inhibitors of PRMTs to treat glioblastoma, medulloblastoma, and other cancers.

Alkaliphilic species Euastrum lacustre (Charophyta, Zygnematophyceae), first record from Russia

2020 ◽  
Vol 54 (2) ◽  
pp. 313-320
Author(s):  
O. V. Anissimova
Keyword(s):  
Cell Wall ◽  
New Species ◽  
First Record ◽  
European Russia ◽  
Similar Species ◽  
Morphological Differences ◽  
First Time ◽  
Kursk Region

Euastrum lacustre is reported for Russia for the first time. This alcaliphilic species was found in the periphyton and plankton of three lakes in the Kursk Region (European Russia). A description of morphology, including the relief of cell wall, and habitats where this taxon is found are represented. LM and SEM microphotographs are provided. Morphological differences of E. lacustre from similar species are discussed. New species for region, namely Closterium aciculare, Cosmarium formosulum, C. granatum, C. pseudoinsigne, C. reniforme and Staurastrum pingue, are found in the samples together with E. lacustre.

scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

scholarly journals The Insect Type 1 Tyramine Receptors: From Structure to Behavior

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 315
Author(s):  
Luca Finetti ◽  
Thomas Roeder ◽  
Girolamo Calò ◽  
Giovanni Bernacchia
Keyword(s):  
Intracellular Signaling ◽  
Physiological Role ◽  
Basic Structure ◽  
Special Focus ◽  
Behavioral Traits ◽  
Physiological Relevance ◽  
Transduction Mechanisms ◽  
Octopamine Receptor ◽  
Available Information ◽  
First Time

Tyramine is a neuroactive compound that acts as neurotransmitter, neuromodulator, and neurohormone in insects. Three G protein-coupled receptors, TAR1-3, are responsible for mediating the intracellular pathway in the complex tyraminergic network. TAR1, the prominent player in this system, was initially classified as an octopamine receptor which can also be activated by tyramine, while it later appeared to be a true tyramine receptor. Even though TAR1 is currently considered as a well-defined tyramine receptor and several insect TAR1s have been characterized, a defined nomenclature is still inconsistent. In the last years, our knowledge on the structural, biochemical, and functional properties of TAR1 has substantially increased. This review summarizes the available information on TAR1 from different insect species in terms of basic structure, its regulation and signal transduction mechanisms, and its distribution and functions in the brain and the periphery. A special focus is given to the TAR1-mediated intracellular signaling pathways as well as to their physiological role in regulating behavioral traits. Therefore, this work aims to correlate, for the first time, the physiological relevance of TAR1 functions with the tyraminergic system in insects. In addition, pharmacological studies have shed light on compounds with insecticidal properties having TAR1 as a target and on the emerging trend in the development of novel strategies for pest control.

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