scholarly journals A preliminary study of the calcification reason of pit mud in Chinese Strong-flavour liquor

2018 ◽  
Author(s):  
Bin Chen ◽  
Deliang Wang ◽  
Yinzhuo Yan
Keyword(s):  
Lactic Acid ◽  
Metal Ions ◽  
High Throughput ◽  
Inductively Coupled Plasma ◽  
High Throughput Sequencing ◽  
Chemical Properties ◽  
Illumina Miseq ◽  
Inductively Coupled ◽  
Pit Mud ◽  
Brewing Process

AbstractPit mud play a key role in the brewing process of Chinese Strong-flavour liquor. In this study, the organic acids and metal ions was determinated by Ion Chromatograph and Inductively Coupled Plasma Mass Spectrometry, respectively. The diversities and structures in pit mud were analyzed by using high-throughput sequencing based on Illumina Miseq platform. The results showed that the lactic acid in calcified pit mud was about 10 times higher than quality pit mud, and the calcium ions was about 20 times higher than quality pit mud. It could found a strange phenomenon, the calcified pit mud had more pyruvic acid and quality pit mud basically does not had this kind of material. The data analysis shown that the pit mud were detected prokaryotes 908 strains and the eukaryotes 226 strains. It was found that the Lactobacillus and Prevotella in calcified pit mud (the total of both increase 11 times) had a higher percentage. It can be guess that the lactic acid and metal ions would form lactic acid salt compounds and those compounds could affect the growth and reproduction of microbes in pit mud. This study provided a good comparison of physical and chemical properties between the calcified pit mud and the quality pit mud, which was significant for the maintenance of pit mud and the quality of liquor. Because of the first study for pit mud by using high-throughput sequencing, it could provide theoretical basis for improving microbes in pit mud.

scholarly journals Ion Release from Dental Implants, Prosthetic Abutments and Crowns under Physiological and Acidic Conditions

Coatings ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
María Arregui ◽  
Florian Latour ◽  
Francisco Javier Gil ◽  
Román A. Pérez ◽  
Luis Giner-Tarrida ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Dental Implants ◽  
Inductively Coupled Plasma ◽  
Ion Concentration ◽  
Ion Release ◽  
Inductively Coupled ◽  
Immersion Medium ◽  
The Media ◽  
Acidic Conditions ◽  
Clinical Conditions

Ion release from dental implants and prosthetic restoration can affect osteointegration and implant viability over a long period of time. Therefore, the aim of this study was to study the ion release from implants and crowns, with and without intermediate anodized abutments, in two different media, simulating clinical conditions. The implants, intermediate prosthetic abutments and Cr–Co crowns were divided into two groups depending on the media: Hanks’ solution and 1% lactic acid, simulating body fluids and microbiologically conditioned fluids, respectively. The study followed the ISO 10271:2011 and 10993-15:2000 standards modified to simulate the replacement of fluids in the oral environment. The ions’ release was measured by inductively coupled plasma mass spectroscopy (ICP-EOS), and only aluminum, chromium, cobalt, titanium and vanadium were identified. Ion concentration was higher in lactic acid than in Hanks’ solution at all time points (p < 0.05). Only vanadium showed a very low ion release in lactic acid, with no statistically significant differences from the ion release in Hanks’ solution (p = 0.524). Both anodized abutments and the immersion medium influenced the release of ions and affected the corrosion of these structures. The presence of an intermediate anodized abutment also affected ion release, as the level of ions was lower in groups with this component.

Analysis of Microbial Community Structure and Volatile Compounds in the Pit Mud Used for Manufacturing Taorong-type Baijiu Based on High-Throughput Sequencing

2021 ◽  
Author(s):  
Yanbo Liu ◽  
Mengxiao Sun ◽  
Pei Hou ◽  
Wenya Wang ◽  
Xiangkun Shen ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Qualitative Analysis ◽  
Volatile Compounds ◽  
High Throughput ◽  
Microbial Community Structure ◽  
High Throughput Sequencing ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Pit Mud

Abstract In this study, the pit mud used in manufacturing Taorong-type Baijiu was collected from the upper, middle, lower and bottom layers of pits in Henan Yangshao Liquor Co., LTD. Besides, high-throughput sequencing (HTS) technology was adopted to analyze the microbial community structure of the pit mud. In addition, the volatile compounds in the pit mud were subjected to preliminarily qualitative analysis through headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The results of HTS demonstrated that there were 5, 3, 5 and 5 dominant bacterial phyla (including 11, 11, 9 and 8 dominant bacterial genera) and 3, 3, 3 and 3 dominant fungal phyla (including 4, 7, 7 and 5 dominant fungal genera) in the pit mud from F-S (upper), G-Z (middle), H-X (lower) and I-D (bottom), respectively. The qualitative analysis results of volatile compounds demonstrated that a total of 78 kinds of volatile compounds were detected in the pit mud, including 46, 45, 39 and 49 kinds in the pit mud from F-S, G-Z, H-X and I-D, respectively. Ester and acid were the two main components in the pit mud. Meanwhile, the correlation between microorganisms and main volatile compounds in the pit mud was analyzed. Moreover, Lentimicrobium, Syner-01 and Blvii28_wastewater-sludge group were found for the first time in the pit mud used for manufacturing Taorong-type Baijiu. The findings of this study could provide a theoretical foundation for improving the quality of pit mud and the flavor of Taorong-type Baijiu.

The development of intestinal lactic acid bacteria in piglets as determined by high-throughput sequencing

2021 ◽  
pp. 1-10
Author(s):  
Lufen Feng ◽  
Chongmiao Zhu ◽  
Pengke Xia ◽  
Hongyu Wang ◽  
Yong Su ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
High Throughput ◽  
High Throughput Sequencing

Analysis of the effects of nanosilver on bacterial community in the intestinal fluid of silkworms using high-throughput sequencing

2019 ◽  
Vol 110 (3) ◽  
pp. 309-320
Author(s):  
Chen Lin ◽  
Zhou Wei ◽  
Zhou Yi ◽  
Tan Tingting ◽  
Du Huamao ◽  
...  
Keyword(s):  
Bacterial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
Illumina Miseq ◽  
The Body ◽  
Dependent Manner ◽  
Negative Effects ◽  
Silkworm Larvae ◽  
Intestinal Bacterial Community

AbstractNanosilver is an environment-friendly, harmless alternative of traditional disinfectants which can be potentially applied in the sericulture industry. However, the effects of nanosilver on the intestinal bacterial community of the silkworms (Bombyx mori L.) are unclear. In this study, Illumina MiSeq high-throughput sequencing technology was used to assess the intestinal bacterial community in both male and female silkworms while treated with different concentrations of nanosilver. We found that nanosilver significantly influenced the composition of silkworm intestinal bacterial community on the different taxonomic levels. Most conspicuously, the abundance of Firmicutes was increased by the treatment of 20 mg L−1 nanosilver but decreased by that of 100 mg L−1 nanosilver at the phylum level. The same trend was observed in Bacilli at the class level and in Enterococcus at the genus level. In some extreme cases, application of nanosilver eliminated the bacterium, e.g., Brevibacillus, but increased the population of several other bacteria in the host intestine, such as Blautia, Terrisporobacter, Faecalibacterium, and some bacteria could only be found in nanosilver treatment groups, e.g., Dialister. In addition, although nanosilver generally showed negative effects on the cocooning rate in a dose-dependent manner, we found that 20 mg L−1 nanosilver treatment significantly increased the body weight of silkworms and did not show negative effects on the survival rate. These results indicated that the intestinal bacteria community of silkworm larvae was significantly changed after nanosilver treatment which might consequently influence host growth and development.

scholarly journals DNA metabarcoding of insects and allies: an evaluation of primers and pipelines

2015 ◽  
Vol 105 (6) ◽  
pp. 717-727 ◽  
Author(s):  
G.-J. Brandon-Mong ◽  
H.-M. Gan ◽  
K.-W. Sing ◽  
P.-S. Lee ◽  
P.-E. Lim ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Illumina Miseq ◽  
Ease Of Use ◽  
Malaise Trap ◽  
Detection Rates ◽  
Operational Taxonomic Units ◽  
Arthropod Species ◽  
Primer Sets ◽  
Arthropod Biodiversity

AbstractMetabarcoding, the coupling of DNA-based species identification and high-throughput sequencing, offers enormous promise for arthropod biodiversity studies but factors such as cost, speed and ease-of-use of bioinformatic pipelines, crucial for making the leapt from demonstration studies to a real-world application, have not yet been adequately addressed. Here, four published and one newly designed primer sets were tested across a diverse set of 80 arthropod species, representing 11 orders, to establish optimal protocols for Illumina-based metabarcoding of tropical Malaise trap samples. Two primer sets which showed the highest amplification success with individual specimen polymerase chain reaction (PCR, 98%) were used for bulk PCR and Illumina MiSeq sequencing. The sequencing outputs were subjected to both manual and simple metagenomics quality control and filtering pipelines. We obtained acceptable detection rates after bulk PCR and high-throughput sequencing (80–90% of input species) but analyses were complicated by putative heteroplasmic sequences and contamination. The manual pipeline produced similar or better outputs to the simple metagenomics pipeline (1.4 compared with 0.5 expected:unexpected Operational Taxonomic Units). Our study suggests that metabarcoding is slowly becoming as cheap, fast and easy as conventional DNA barcoding, and that Malaise trap metabarcoding may soon fulfill its potential, providing a thermometer for biodiversity.

Preparation of Silica-Doped Poly(Lactic Acid) Composite Hollow Spheres Containing Calcium Carbonates

2006 ◽  
Vol 309-311 ◽  
pp. 457-460 ◽  
Author(s):  
Noriko Miura ◽  
Hirotaka Maeda ◽  
Toshihiro Kasuga
Keyword(s):  
Lactic Acid ◽  
Inductively Coupled Plasma ◽  
Hollow Spheres ◽  
Spherical Shape ◽  
Atomic Emission ◽  
Poly Lactic Acid ◽  
External Diameter ◽  
Calcium Carbonates ◽  
Inductively Coupled ◽  
A Cell

Silica-doped poly(lactic acid) (PLA) composite hollow spheres containing calcium carbonates (Si-CCPC spheres) were prepared using aminopropyltriethoxysilane (APTES) for injectable bone fillers combined with a cell-delivery system. Si-CCPC spheres have a hollow spherical shape of ~1 mm in the external diameter and an open channel in the shell, which is selfformed. The channel size is about 500 *m in diameter. X-ray energy dispersive spectroscopy (EDS) analysis showed incorporation of silicon in Si-CCPC spheres. After soaking Si-CCPC spheres in simulated body fluid (SBF), hydroxycarbonate apatite formed on the Si-CCPC spheres. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) showed that the Si4+ ion is released from Si-CCPC spheres in SBF.

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