scholarly journals Cryopreservation and transplantation of common carp spermatogonia

2018 ◽  
Author(s):  
Roman Franěk ◽  
Zoran Marinović ◽  
Jelena Lujić ◽  
Béla Urbányi ◽  
Michaela Fučíková ◽  
...  
Keyword(s):  
Common Carp ◽  
Sperm Cryopreservation ◽  
Post Transplantation ◽  
Wide Range ◽  
Cultured Fish ◽  
The World ◽  
Exogenous Origin ◽  
Long Term Preservation ◽  
Surrogate Production

AbstractCommon carp (Cyprinus carpio) is one of the most cultured fish species over the world with many different breeds and plenty of published protocols for sperm cryopreservation, however, data regarding preservation of gonadal tissue and surrogate production is still missing. A protocol for freezing common carp spermatogonia was developed through varying different factors along a set of serial subsequent experiments. Among the six cryoprotectants tested, the best survival was achieved with dimethyl sulfoxide (Me2SO). In the next experiment, a wide range of cooling rates (0.5–10 °C/min) and different concentrations of Me2SO were tested resulting in the highest survival using 2 M Me2SO and cooling rate of –1 Q59 A When testing different tissue sizes and incubation times in the cryomedium, the highest viability was observed when incubating 100 mg tissue fragments for 30 min. Finally, sugar supplementation did not yield significant differences. When testing different equilibration (ES) and vitrification solutions (VS) used for needle-immersed vitrification, no significant differences were observed between the tested groups. Additionally, varied exposure time to VS did not improve the vitrification outcome where the viability was 4-fold lower than that of freezing. The functionality of cryopreserved cells was tested by interspecific transplantation into sterilized goldfish recipients. The exogenous origin of the gonads in goldfish recipients was confirmed by molecular markers and incorporation rate was over 40% in both groups at 3 months post transplantation. Results of this study can serve as an alternative way for long-term preservation of germplasm in carp which can be recovered in a surrogate recipient.

scholarly journals Clinical case of acute hepatitis caused by parvovirus B 19

2021 ◽  
Vol 13 (2) ◽  
pp. 154-158
Author(s):  
K. V. Sobolevskiy ◽  
I. A. Otmakhova ◽  
B. Ya. Gadirova ◽  
Zh. E. Zueva
Keyword(s):  
Acute Hepatitis ◽  
Parvovirus B19 ◽  
Clinical Manifestations ◽  
Viral Persistence ◽  
Fetal Infection ◽  
Wide Range ◽  
The World ◽  
Parvovirus B19 Infection ◽  
Parvovirus B 19

Parvovirus infection is one of the most widespread infectious diseases in the world. Parvovirus В19 can cause a wide range of clinical manifestations with possible long-term viral persistence. Infectious erythema, arthropathy, fetal infection, and blood cell aplasia are the most common manifestations of parvovirus infection. A case of diagnosis and treatment of a rare variant of parvovirus B19 infection that occurred as acute hepatitis is presented. 

Preservation and Access to Digital Materials

2018 ◽  
pp. 150-174
Author(s):  
Trywell Kalusopa
Keyword(s):  
Literature Review ◽  
Learning Curve ◽  
Policy Options ◽  
African Countries ◽  
Digital World ◽  
Collaborative Strategies ◽  
The World ◽  
Developed World ◽  
Long Term Preservation

As the digital world unfolds, Africa continues to grapple with the issues of preservation and access of digital materials. This chapter demonstrates through a systematic literature review how Africa could learn from other efforts in the world to develop and guide their own strategic and policy options to deal with issues of preservation and access of digital materials. The chapter reviews literature on global or universal collaborative strategies and efforts on digital preservation initiated in the developed world as a learning curve for Africa. The current challenges of national and institutional capacities regarding the preservation of digital materials in selected African countries that have made some visible efforts and impact are also discussed. The chapter then makes several recommendations on the strategic and policy options for improving the state of material preservation, human and material requirements in order to improve the long-term preservation, and standards for the longer-term usability and interoperability of digital materials in Africa.

scholarly journals Beyond the vigilant state: globalisation and intelligence

2009 ◽  
Vol 35 (4) ◽  
pp. 889-902 ◽  
Author(s):  
RICHARD J. ALDRICH
Keyword(s):  
Civil Society ◽  
Military Power ◽  
Structural Factors ◽  
Ethical Practice ◽  
Intelligence Services ◽  
Wide Range ◽  
The World ◽  
Globalising World ◽  
New Terrorism

AbstractThe world of intelligence has grown exponentially over the last decade. This article suggests that prevailing explanation of this expansion – the spectre of ‘new terrorism’ – reflects serious misunderstandings. Much of the emergency legislation which has extended the power of the state so remarkably was already sitting in the pending trays of officials in the late 1990s. Instead, the rise of both the ‘new terrorism’ and its supposed nemesis – the secret state – both owe more to long-term structural factors. Globalisation has accelerated a wide range of sub-military transnational threats, of which the ‘new terrorism’ is but one example. Meanwhile the long-promised engines of global governance are nowhere in sight. In their absence, the underside of a globalising world is increasingly policed by ‘vigilant states’ that resort to a mixture of military power and intelligence power in an attempt to address these problems. Yet the intelligence services cannot meet the improbable demands for omniscience made by governments, nor can they square their new enforcer role with vocal demands by global civil society for improved ethical practice.

scholarly journals The Homogocene: a research prospectus for the study of biotic homogenisation

NeoBiota ◽  
2018 ◽  
Vol 37 ◽  
pp. 23-36 ◽  
Author(s):  
Julian D. Olden ◽  
Lise Comte ◽  
Xingli Giam
Keyword(s):  
Global Change ◽  
Biological Diversity ◽  
Significant Progress ◽  
Temporal Scales ◽  
Spatial And Temporal Scales ◽  
Conservation Implications ◽  
The World ◽  
Biotic Homogenisation ◽  
Long Term Preservation

In an era of global change, the process of biotic homogenisation by which regional biotas become more similar through time has attracted considerable attention from ecologists. Here, a retrospective look at the literature is taken and the question asked how comprehensive is the understanding of this global phenomenon? The goal is to identify potential areas for additional and future enquiries to advance this research frontier and best ensure the long-term preservation of biological diversity across the world. Six propositions are presented here to; (1) broaden our geographic and taxonomic understanding, (2) diversify the spatial and temporal scales of inquiry, (3) reconcile past and embrace new approaches to quantification, (4) improve our knowledge of the underlying drivers, (5) reveal the conservation implications and (6) forecast future homogenisation. It is argued that significant progress in the understanding of the causes, consequences and conservation implication of biotic homogenisation will come by integrating concepts and approaches from ecology, evolution and conservation across a hierarchy of spatial and temporal scales.

Preservation and Access to Digital Materials

2018 ◽  
pp. 447-471
Author(s):  
Trywell Kalusopa
Keyword(s):  
Literature Review ◽  
Learning Curve ◽  
Policy Options ◽  
African Countries ◽  
Digital World ◽  
Collaborative Strategies ◽  
The World ◽  
Developed World ◽  
Long Term Preservation

As the digital world unfolds, Africa continues to grapple with the issues of preservation and access of digital materials. This chapter demonstrates through a systematic literature review how Africa could learn from other efforts in the world to develop and guide their own strategic and policy options to deal with issues of preservation and access of digital materials. The chapter reviews literature on global or universal collaborative strategies and efforts on digital preservation initiated in the developed world as a learning curve for Africa. The current challenges of national and institutional capacities regarding the preservation of digital materials in selected African countries that have made some visible efforts and impact are also discussed. The chapter then makes several recommendations on the strategic and policy options for improving the state of material preservation, human and material requirements in order to improve the long-term preservation, and standards for the longer-term usability and interoperability of digital materials in Africa.

scholarly journals A Review on the Historical Development of Environmental Specimen Banks

2018 ◽  
Vol 38 ◽  
pp. 01016
Author(s):  
Yu Xin Luo
Keyword(s):  
Historical Development ◽  
Environmental Studies ◽  
Current Status ◽  
Environmental Specimen ◽  
The World ◽  
Long Term Preservation ◽  
Monitoring Practice ◽  
Environmental Specimen Banks

Environmental Specimen Banks (ESBs) are facilities that archive samples from the environment for future researching and monitoring purposes. In addition, the long-term preservation of representative specimens is an important complement to environmental studies and monitoring practice. This article presented detailed information about ESBs in the world, and reviewed the history and current status of ESBs in China. Besides, crucialshould leave 8 mm of space above the abstract and 10 mm after the abstract.

Paleoecological and stratigraphic controls on eurypterid Lagerstätten: a model for preservation in the mid-Paleozoic

Paleobiology ◽  
2017 ◽  
Vol 43 (3) ◽  
pp. 383-406 ◽  
Author(s):  
Matthew B. Vrazo ◽  
Carlton E. Brett ◽  
Samuel J. Ciurca
Keyword(s):  
Lower Devonian ◽  
Abiotic Conditions ◽  
Sequence Stratigraphic ◽  
Exceptional Preservation ◽  
Microbial Structures ◽  
Subaerial Exposure ◽  
Stratigraphic Framework ◽  
The World ◽  
Long Term Preservation

AbstractRecent studies of eurypterid paleoecology suggest that formation of eurypterid Lagerstätten in the mid-Paleozoic of Laurentia was controlled by the presence of an ecological–taphonomic window that recurred predictably in nearshore, marginal environments during transgressions. We tested this hypothesis by performing a high-resolution taxonomic, environmental, and stratigraphic survey and quantitative analysis of all Silurian–Lower Devonian eurypterid-bearing intervals in the Appalachian basin, the most prolific region for eurypterid remains in the world. Canonical correspondence analysis of sedimentological and faunal associations revealed a strong lithologic gradient between groupings of eurypterid genera and associated taxa across the basin, and a significant association of eurypterids with microbialites (thrombolites, stromatolites) and evaporitic structures. Field observations confirmed that, stratigraphically, eurypterids in the basin frequently occur above the microbialite structures and beneath evaporites and other indicators of increased salinity or subaerial exposure. Following interpretation of these features within a sequence stratigraphic framework, we present a preservational model in which (1) eurypterids inhabited nearshore settings following freshening conditions concomitant with minor transgressions, (2) their remains were subsequently buried by storms or microbialite sediment baffling, and (3) subsequent long-term preservation of tissues was facilitated by regression and cyclical shallowing-up successions that promoted hypersalinity and anoxia. In the central and southern region of the basin, where microbial structures and evidence for hypersalinity are less common, a similar pattern of cyclical shallowing-upward deposition within eurypterid-bearing units holds. Thus, eurypterid preservation appears to reflect a combination of ecological preferences and abiotic conditions that promoted inhabitation and eventual preservation within the same setting. This study provides the first quantitative support for a sea level–based control on preservation of eurypterids and adds to the growing body of evidence that suggests that analysis of exceptional preservation in the fossil record benefits from interpretation within a sequence stratigraphic framework.

scholarly journals Development of a sperm cryopreservation protocol of Gangetic Mystus (Mystus cavasius)

2017 ◽  
Vol 27 (4) ◽  
pp. 517-529
Author(s):  
MM Islam Islam ◽  
MN Noor ◽  
AA Islam ◽  
MRI Sarder ◽  
MZ Islam ◽  
...  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Sperm Motility ◽  
Genetic Material ◽  
Nacl Solution ◽  
Sperm Cryopreservation ◽  
Endangered Fish ◽  
Long Term Preservation ◽  
Cryopreservation Protocol ◽  
Near Threatened

Cryopreservation is considered as one of the most useful techniques for long-term preservation of genetic material specially sperm of fish. This study focused on the development of a sperm cryopreservation protocol for indigenous near threatened gulsha (Mystus cavasius) and a number of experiments were conducted for the purpose. To collect milt, male gulsha were sacrificed and milt was suspended in extenders. Different concentrations of NaCl were used to evaluate the activation of sperm motility and it decreased as the concentration of the extending media increased, therefore, motility was completely inhibited at 0.8% and 1.2% NaCl solution when sperm suspended in Kurokura-2 and Alsever’s solution, respectively. The toxicity of cryoprotectants to sperm were evaluated using two cryoprotectants, dimethyl sulfoxide (DMSO) and methanol along with the extenders, Alsever’s solution and Kurokura-2 solution. DMSO and methanol with 5% and 10% concentrations produced significantly higher motility during 5 and 10 min incubation and their 15% concentration found toxic to sperm. Alsever’s solution with 10% DMSO produced best equilibration (83.75±2.39%) as well as post-thaw motility (67.5±3.23%) while Kurokura-2 solution with DMSO produced similar equilibration motility (81.25±2.39%) but the post-thaw motility (50.0±6.12%) was significantly much lower than that of Alsever’s solution. Sperm preserved with Alsever’s solution plus DMSO produced highest fertilization, 72.5±7.5% and hatching, 56.8±5.6% while fresh sperm yielded 85.0±5.0% and 74.8±3.6% fertilization and hatching, respectively. The protocols that have been developed can be used for conservation of genetic materials of M. cavasius and other endangered fish species and new generations of them can be propagated using the cryopreserved sperm.Progressive Agriculture 27 (4): 517-529, 2016

Manufacturing Research with Feasibility of Vacuum Freeze Drying Technology for Leisure Meat Products Processing

2014 ◽  
Vol 1056 ◽  
pp. 84-87
Author(s):  
Yan Ma ◽  
Wei Wei Liu ◽  
Guo Hui Huang
Keyword(s):  
Freeze Drying ◽  
Meat Products ◽  
Nutrient Loss ◽  
Light Weight ◽  
Technology Research ◽  
Drying Method ◽  
Wide Range ◽  
Drying Technology ◽  
Long Term Preservation

vacuum freeze drying technology is a high technology content ,involving a wide range of knowledge of technology in the field of drying technology, it is also a method of the most complex drying equipment , the largest energy consumption, the highest cost of drying method, but due to the particularity of its dry goods: the freeze-drying food has the advantages of complex water performance is good, cooler and luster of freezing and drying food to maintain good products, less nutrient loss, light weight, easy to carry transportation, easy to long-term preservation, and on the quality is far superior to the obvious advantages of other dried food, making it become the forefront of drying technology research and development.

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