scholarly journals Estimation of the Hemoglobin Glycation Rate Constant

2019 ◽  
Author(s):  
Masashi Kameyama ◽  
Toshika Okumiya ◽  
Shinji Tokuhiro ◽  
Yoshihisa Matsumura ◽  
Hirotaka Matsui ◽  
...  
Keyword(s):  
Case Studies ◽  
Hemolytic Anemia ◽  
Rate Constant ◽  
Plasma Glucose ◽  
Accurate Estimate ◽  
True Value ◽  
A Value ◽  
Erythrocyte Age ◽  
Hemoglobin Glycation

AbstractAimIn a previous study, a method of obtaining mean erythrocyte age (MRBC) from HbA1c and average plasma glucose (AG) was proposed. However, the true value of the hemoglobin glycation constant (kgdL/mg/day), required for this model has yet to be well characterized. Another study also proposed a method of derivingMRBCfrom erythrocyte creatine (EC). Utilizing these formulae, this study aimed to determine a more accurate estimate ofkg.Methods107 subjects including 31 patients with hemolytic anemia and 76 subjects without anemia were included in this study. EC and HbA1c data were analyzed, andMRBCusing HbA1c, AG and the newly-derived constant,kgwere compared toMRBCusing traditional51Cr in three patients whose data were taken from previous case studies.ResultA value of 7.0 × 10−6dL/mg/day was determined forkg.MRBCusing HbA1c, AG andkgwere found to no be significantly different (pairedt-test,p= 0.45) toMRBCusing traditional51Cr.Conclusionskgenables the estimation ofMRBCfrom HbA1c and AG.

scholarly journals Estimation of the hemoglobin glycation rate constant

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masashi Kameyama ◽  
Toshika Okumiya ◽  
Shinji Tokuhiro ◽  
Yoshihisa Matsumura ◽  
Hirotaka Matsui ◽  
...  
Keyword(s):  
Case Studies ◽  
Hemolytic Anemia ◽  
Rate Constant ◽  
Plasma Glucose ◽  
Accurate Estimate ◽  
Formula One ◽  
True Value ◽  
A Value ◽  
Erythrocyte Age ◽  
Hemoglobin Glycation

AbstractIn a previous study, a method of obtaining mean erythrocyte age ($$M_{RBC}$$ M RBC ) from HbA1c and average plasma glucose (AG) was proposed. However, the true value of the hemoglobin glycation constant ($$k_g$$ k g dL/mg/day), required for this model has yet to be well characterized. Another study also proposed a method of deriving $$M_{RBC}$$ M RBC from erythrocyte creatine (EC). Utilizing these formulae, this study aimed to determine a more accurate estimate of $$k_g$$ k g . One hundred and seven subjects including 31 patients with hemolytic anemia and 76 subjects without anemia were included in this study. EC and HbA1c data were analyzed, and $$M_{RBC}$$ M RBC using HbA1c, AG and the newly-derived constant, $$k_g$$ k g were compared to $$M_{RBC}$$ M RBC using traditional $$^{51}\hbox {Cr}$$ 51 Cr in three patients whose data were taken from previous case studies. A value of $$7.0\times 10^{-6}$$ 7.0 × 10 - 6 dL/mg/day was determined for $$k_g$$ k g . $$M_{RBC}$$ M RBC using HbA1c, AG and $$k_g$$ k g were found to no be significantly different (paired t-test, $$p=0.45$$ p = 0.45 ) to $$M_{RBC}$$ M RBC using traditional $$^{51}\hbox {Cr}$$ 51 Cr . $$k_g$$ k g enables the estimation of $$M_{RBC}$$ M RBC from HbA1c and AG.

Hemoglobin Glycation Rate Constant in Non-diabetic Individuals

2011 ◽  
Vol 39 (11) ◽  
pp. 2721-2734 ◽  
Author(s):  
Piotr Ładyżyński ◽  
Jan M. Wójcicki ◽  
Marianna I. Bąk ◽  
Stanisława Sabalińska ◽  
Jerzy Kawiak ◽  
...  
Keyword(s):  
Rate Constant ◽  
Hemoglobin Glycation

scholarly journals Determination of the Value of Intangible Assets in the Companies of Lithuania

2017 ◽  
Vol 14 (2) ◽  
pp. 55-68 ◽  
Author(s):  
Rita Bužinskienė
Keyword(s):  
Financial Management ◽  
Intangible Assets ◽  
Fair Value ◽  
Financial Information ◽  
Financial Accounting ◽  
True Value ◽  
A Value ◽  
The Impact

AbstractIn accordance with generally accepted accounting standards, most intangibles are not accounted for and not reflected in the traditional financial accounting. For this reason, most companies account intangible assets (IAs) as expenses. In the research, 57 sub-elements of IAs were applied, which are grouped into eight main elements of IAs. The classification of IAs consists in two parts of assets: accounting and non-accounting. This classification can be successfully applied in different branches of enterprises, to expand and supplement the theoretical and practical concepts of the company's financial management. The article proposes to evaluate not only the value of financial information for IAs (accounted) but also the value of non-financial information for IAs (non-accounted), thus revealing the true value of IAs that is available to the companies of Lithuania. It names a value of general IAs. The results of the research confirmed the IA valuation methodology, which allows companies to calculate the fair value of an IA. The obtained extended IAs valuation information may be valuable to both the owners of the company and investors, as this value plays an important practical role in assessing the impact of IAs on the market value of companies.

scholarly journals Uncertainty Estimation of the Fe Content Measurement in the Clean Water Using UV- Vis Spectrophotometer

2019 ◽  
Vol 1 (1) ◽  
pp. 1-5
Author(s):  
Adi Syahputra
Keyword(s):  
Quality Standards ◽  
Sample Volume ◽  
Uncertainty Estimation ◽  
Clean Water ◽  
True Value ◽  
A Value ◽  
Fe Content ◽  
Standard Solution ◽  
Range Of Values ◽  
Orange Solution

Good laboratories should be able to apply procedures uncertainty for measuring of both conventional and instrument equipment. As well as the testing for Fe content using UV-Vis Spectrophotometer. As for the purpose for calculating the uncertainty estimated to determine the range of values within which estimated the true value of the testing standard solution Fe using UV-Vis Spectrophotometer. The uncertainty estimation are influenced by several factors such as sample used, reading of spectrophotometer, repeatability, sample volume. The uncertainty of the calculation obtained a value of ± 0,02 mg/L. Further testing of Fe content in the clean water using UV-Vis Spectrophotometer  at the 500 nm wavelength which forms an orange solution as indication of Fe content in the water. The result of Fe content has quality standards according to the spesifications issued by ministry of health Republic of Indonesia  in 2010 for drinking water.

Direct spectrophotometry of magnesium in serum after reaction with hexokinase and glucose-6-phosphate dehydrogenase.

1985 ◽  
Vol 31 (5) ◽  
pp. 703-705 ◽  
Author(s):  
M Tabata ◽  
T Kido ◽  
M Totani ◽  
T Murachi
Keyword(s):  
Spectrophotometric Method ◽  
Present Method ◽  
Reaction Rate ◽  
Colorimetric Method ◽  
Simple Method ◽  
True Value ◽  
A Value ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Direct Spectrophotometry

Abstract We describe a simple method for determining magnesium in serum by using hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The method is based on determination of the reaction rate of hexokinase activated by Mg2+, which participates in the hexokinase reaction as the substrate in the form of a Mg X ATP2- complex. The reaction rate is determined from the change in absorbance at 340 nm as NADPH is produced by the action of glucose-6-phosphate dehydrogenase. This simple and rapid spectrophotometric method does not require expensive instrumentation, but results correlate satisfactorily with those obtained by atomic absorption spectroscopy. Thus, the present method gives a "true" value for magnesium in serum, a value appreciably lower than that obtained by an earlier colorimetric method, the Xylidyl Blue II method (Biochem Med 7: 208-217, 1973), which lacks specificity.

scholarly journals Are the Fractionation Corrections Correct: Are the Isotopic Shifts for 14C/12C Ratios in Physical Processes and Chemical Reactions Really Twice Those for 13C/12C?

Radiocarbon ◽  
2011 ◽  
Vol 53 (4) ◽  
pp. 691-704 ◽  
Author(s):  
John Southon
Keyword(s):  
Chemical Reactions ◽  
Isotopic Fractionation ◽  
Theoretical Work ◽  
Chemical Processes ◽  
Physical Processes ◽  
Isotopic Discrimination ◽  
True Value ◽  
A Value ◽  
Isotopic Shifts ◽  
Physical And Chemical

Conventional radiocarbon calculations correct for isotopic fractionation using an assumed value of 2.0 for the fractionation of 14C relative to 13C. In other words, isotopic discrimination in physical and chemical processes is assumed to cause relative shifts in 14C/12C ratios that are exactly double those of 13C/12C. This paper analyzes a 1984 experiment that produced a value for the fractionation ratio in photosynthesis of 2.3, which is used to this day by some researchers in the fields of hydrology and speleothem geochemistry. While the value of 2.3 is almost certainly incorrect, theoretical work suggests that the true value may indeed deviate from 2.0, which would have significant implications for 14C calculations.

scholarly journals Blood flow to bone marrow during development of anemia or polycythemia in the rat

Blood ◽  
1992 ◽  
Vol 79 (3) ◽  
pp. 594-601 ◽  
Author(s):  
PO Iversen ◽  
G Nicolaysen ◽  
HB Benestad
Keyword(s):  
Bone Marrow ◽  
Blood Flow ◽  
Hemolytic Anemia ◽  
Time Course ◽  
Relative Increase ◽  
Detectable Effect ◽  
A Value ◽  
Microsphere Method ◽  
Radioactive Microsphere Method ◽  
Increased Blood Flow

Abstract We applied the radioactive microsphere method to follow the magnitude and time course (0 to 96 hours) of blood flow changes during development and recovery from anemia in awake rats. Blood flow was also monitored during a 96-hour period after polycythemia was induced (erythropoietin administered subcutaneously [SC]). The possible influence of innervation was also examined. After a blood loss of approximately 50% (hypovolemia), blood flow to the femoral marrow tripled within 12 hours and remained elevated for the entire 96-hour period. The relative increase in blood flow to the femoral bone was even greater. Similar findings were obtained in rats with phenylhydrazine (PHZ) hemolytic anemia (normovolemia). Denervation had no detectable effect on the increased blood flow to either marrow or bone. The augmented blood flow during hemolytic anemia was accompanied by a doubling of the oxygen consumption rate by the marrow, while the glucose uptake was not detectably altered. Erythropoietin supplements (3 x 1,000 IU/kg, SC, 6-hour intervals) increased blood flow to the marrow by approximately 25% after 48 hours, and at 72 hours the blood flow had reached a value twice that obtained under control conditions. These results indicate that blood flow to bone marrow is highly variable and hormonally and/or locally regulated. This may have practical consequences for marrow transplantation technology and for administration of drug therapy to patients with insufficient bone marrow hematopoiesis.

Kinetic characterization of a cis- and trans-acting M2+-independent DNAzyme that depends on synthetic RNaseA-like functionality — Burst-phase kinetics from the coalescence of two active DNAzyme folds

2007 ◽  
Vol 85 (4) ◽  
pp. 313-329 ◽  
Author(s):  
Richard Ting ◽  
Jason M Thomas ◽  
David M Perrin
Keyword(s):  
Steady State ◽  
Rate Constant ◽  
Acid Catalyst ◽  
Initial Population ◽  
Substrate Complex ◽  
Single Turnover ◽  
A Value ◽  
Burst Phase ◽  
Cis And Trans

This work describes the kinetics of the DNAzyme 925-11, a combinatorially selected, M2+-independent ribophosphodiesterase that is covalently modified with both cationic amines and imidazoles. At 13 °C, cis- and trans-cleaving constructs of 925-11 demonstrate the highest rate constants reported to date for any M2+-independent nucleic acid catalyst, investigated at physiological ionic strength and pH 7.5 (0.3 min–1 for self cleavage and 0.2 min–1 for intermolecular cleavage). In contrast to the cis-cleaving species, single-turnover experiments with the trans-cleaving species exhibit biphasic cleavage data, suggesting the presence of two conformations of the catalyst–substrate complex. Pulse–chase experiments demonstrate that both complexes lead to substrate cleavage. Under multiple-turnover conditions, the higher rate constant appears in a burst phase that decays to a slower steady state exhibiting a rate constant of 0.0077 min–1, a value approximating that of the slow-cleaving phase seen in single-turnover experiments. Slow product release is excluded as the source of the burst phase. An integrated rate equation is derived to describe burst-phase kinetics based on the funneling of the initial population of fast-cleaving conformation into a steady-state population composed largely of the slow-cleaving conformation.Key words: RNase mimics, DNAzymes, ribozymes, kinetics, RNA cleavage.

Human RhAG ammonia channel is impaired by the Phe65Ser mutation in overhydrated stomatocytic red cells

2012 ◽  
Vol 302 (2) ◽  
pp. C419-C428 ◽  
Author(s):  
Sandrine Genetet ◽  
Pierre Ripoche ◽  
Julien Picot ◽  
Sylvain Bigot ◽  
Jean Delaunay ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Hemolytic Anemia ◽  
Rate Constant ◽  
Rate Constants ◽  
Flow Analysis ◽  
Red Cells ◽  
Stopped Flow ◽  
Loss Of Function ◽  
Dramatic Decrease ◽  
Time Courses

In red cells, Rh-associated glycoprotein (RhAG) acts as an ammonia channel, as demonstrated by stopped-flow analysis of ghost intracellular pH (pHi) changes. Recently, overhydrated hereditary stomatocytosis (OHSt), a rare dominantly inherited hemolytic anemia, was found to be associated with a mutation (Phe65Ser or Ile61Arg) in RHAG. Ghosts from the erythrocytes of four of the OHSt patients with a Phe65Ser mutation were resealed with a pH-sensitive probe and submitted to ammonium gradients. Alkalinization rate constants, reflecting NH3transport through the channel and NH3diffusion unmediated by RhAG, were deduced from time courses of fluorescence changes. After subtraction of the constant value found for Rhnulllacking RhAG, we observed that alkalinization rate constant values decreased ∼50% in OHSt compared with those of controls. Similar RhAG expression levels were found in control and OHSt. Since half of the expressed RhAG in OHSt most probably corresponds to the mutated form of RhAG, as expected from the OHSt heterozygous status, this dramatic decrease can be therefore related to the loss of function of the Phe65Ser-mutated RhAG monomer.

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