scholarly journals Draft genome sequences of five Proteobacteria isolated from Lechuguilla Cave, New Mexico, USA: Insights into taxonomy and quorum-sensing

2019 ◽  
Author(s):  
Han Ming Gan ◽  
Peter C. Wengert ◽  
Hazel A. Barton ◽  
André O. Hudson ◽  
Michael A. Savka
Keyword(s):  
New Mexico ◽  
Quorum Sensing ◽  
Draft Genome ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Genome Sequences ◽  
Genomic Resources ◽  
Distinct Genus ◽  
Genus Two

AbstractGenomic resources remain scarce for bacteria isolated from oligotrophic caves. We sequenced the genomes of five Proteobacteria isolated from Lechuguilla Cave in New Mexico, USA. Genome-based phylogeny indicates that each strain belongs to a distinct genus. Two Rhizobiaceae isolates possess the genomic potential for the biosynthesis of acyl-homoserine lactone.

scholarly journals Draft Genome Sequences of Five Proteobacteria Isolated from Lechuguilla Cave, New Mexico, USA, and Insights into Taxonomy and Quorum Sensing

2019 ◽  
Vol 8 (40) ◽  
Author(s):  
Han Ming Gan ◽  
Peter C. Wengert ◽  
Hazel A. Barton ◽  
André O. Hudson ◽  
Michael A. Savka
Keyword(s):  
New Mexico ◽  
Quorum Sensing ◽  
Draft Genome ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Genome Sequences ◽  
Genomic Resources ◽  
Distinct Genus ◽  
Genus Two

Genomic resources remain scarce for bacteria isolated from oligotrophic caves. We sequenced the genomes of five Proteobacteria isolated from Lechuguilla Cave in New Mexico. Genome-based phylogeny indicates that each strain belongs to a distinct genus. Two Rhizobiaceae isolates possess genomic potential for the biosynthesis of acyl-homoserine lactone.

scholarly journals Draft Genome Sequence of Nitrobacter vulgaris Strain Ab1, a Nitrite-Oxidizing Bacterium

2017 ◽  
Vol 5 (18) ◽  
Author(s):  
Brett L. Mellbye ◽  
Edward W. Davis ◽  
Eva Spieck ◽  
Jeff H. Chang ◽  
Peter J. Bottomley ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Genome Sequence ◽  
Draft Genome ◽  
Homoserine Lactone ◽  
Draft Genome Sequence ◽  
Nitrifying Bacteria ◽  
Acyl Homoserine Lactone ◽  
Content Type ◽  
Sewage System ◽  
Nitrite Oxidizing Bacteria

ABSTRACT Here, we present the 3.9-Mb draft genome sequence of Nitrobacter vulgaris strain Ab1, which was isolated from a sewage system in Hamburg, Germany. The analysis of its genome sequence will contribute to our knowledge of nitrite-oxidizing bacteria and acyl-homoserine lactone quorum sensing in nitrifying bacteria.

scholarly journals Genome sequencing-assisted identification and the first functional validation ofN-acyl-homoserine-lactone synthases from the Sphingomonadaceae family

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2332 ◽  
Author(s):  
Han Ming Gan ◽  
Lucas K. Dailey ◽  
Nigel Halliday ◽  
Paul Williams ◽  
André O. Hudson ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Thin Layer ◽  
Heterologous Expression ◽  
Thin Layer Chromatography ◽  
Draft Genome ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Two Dimensional ◽  
Esi Ms ◽  
Layer Chromatography

BackgroundMembers of the genusNovosphingobiumhave been isolated from a variety of environmental niches. Although genomics analyses have suggested the presence of genes associated with quorum sensing signal production e.g., theN-acyl-homoserine lactone (AHL) synthase (luxI) homologs in variousNovosphingobiumspecies, to date, noluxIhomologs have been experimentally validated.MethodsIn this study, we report the draft genome of theN-(AHL)-producing bacteriumNovosphingobium subterraneumDSM 12447 and validate the functions of predictedluxIhomologs from the bacterium through inducible heterologous expression inAgrobacterium tumefaciensstrain NTL4. We developed a two-dimensional thin layer chromatography bioassay and used LC-ESI MS/MS analyses to separate, detect and identify the AHL signals produced by theN. subterraneumDSM 12447 strain.ResultsThree predicted luxI homologs were annotated to the locus tags NJ75_2841 (NovINsub1), NJ75_2498 (NovINsub2), and NJ75_4146 (NovINsub3). Inducible heterologous expression of eachluxIhomologs followed by LC-ESI MS/MS and two-dimensional reverse phase thin layer chromatography bioassays followed by bioluminescent ccd camera imaging indicate that the three LuxI homologs are able to produce a variety of medium-length AHL compounds. New insights into the LuxI phylogeny was also gleemed as inferred by Bayesian inference.DiscussionThis study significantly adds to our current understanding of quorum sensing in the genusNovosphingobiumand provide the framework for future characterization of the phylogenetically interesting LuxI homologs from members of the genusNovosphingobiumand more generally the family Sphingomonadaceae.

scholarly journals Insights into the Genome Sequences of an N -Acyl Homoserine Lactone Molecule Producing Two Pseudomonas spp. Isolated from the Arctic

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Akhilandeswarre Dharmaprakash ◽  
Dinesh Reghunathan ◽  
Krishnakutty C. Sivakumar ◽  
Manoj Prasannakumar ◽  
Sabu Thomas
Keyword(s):  
Quorum Sensing ◽  
Draft Genome ◽  
Homoserine Lactone ◽  
The Arctic ◽  
Acyl Homoserine Lactone ◽  
Cold Environments ◽  
Sensing System ◽  
Quorum Sensing System ◽  
First Time

We report for the first time the draft genome sequence of two psychrotrophic Pseudomonas species, Pseudomonas simiae RGCB 73 and Pseudomonas brenneri RGCB 108, from the Arctic that produce more than one acyl homoserine lactone molecule of varied N -acyl length. The study confirms the presence of a LuxR-LuxI (type) mediated quorum-sensing system in both the Pseudomonas species and enables us to understand the role of quorum sensing in their survival in extremely cold environments.

scholarly journals Draft Genome Sequence ofChromobacterium violaceumStrain CV017

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Xiaofei Wang ◽  
Kara C. Hinshaw ◽  
Stuart J. Macdonald ◽  
Josephine R. Chandler
Keyword(s):  
Quorum Sensing ◽  
Genome Sequence ◽  
Draft Genome ◽  
Homoserine Lactone ◽  
Chromobacterium Violaceum ◽  
Draft Genome Sequence ◽  
Acyl Homoserine Lactone

We announce the draft genome sequence forChromobacterium violaceumstrain CV017, used as a model and tool to understand acyl-homoserine lactone-dependent quorum sensing. The assembly consists of 4,774,638-bp contained in 211 scaffolds.

Biofilm Formation, Communication and Interactions of Mesophilic Leaching Bacteria during Pyrite Oxidation

2013 ◽  
Vol 825 ◽  
pp. 107-110
Author(s):  
Sören Bellenberg ◽  
Robert Barthen ◽  
Mario Vera ◽  
Nicolas Guiliani ◽  
Wolfgang Sand
Keyword(s):  
Quorum Sensing ◽  
Acidithiobacillus Ferrooxidans ◽  
Biofilm Formation ◽  
Pyrite Oxidation ◽  
Cell Communication ◽  
Homoserine Lactone ◽  
Mixed Cultures ◽  
Acyl Homoserine Lactone ◽  
Leaching Bacteria ◽  
Leaching Efficiency

A functional luxIR-type Quorum Sensing (QS) system is present in Acidithiobacillus ferrooxidans. However, cell-cell communication among various acidophilic chemolithoautotrophs growing on pyrite has not been studied in detail. These aspects are the scope of this study with emphasis on the effects exerted by the N-acyl-homoserine lactone (AHL) type signaling molecules which are produced by Acidithiobacillus ferrooxidans. Their effects on attachment and leaching efficiency by other leaching bacteria, such as Acidithiobacillus ferrivorans, Acidiferrobacter spp. SPIII/3 and Leptospirillum ferrooxidans in pure and mixed cultures growing on pyrite is shown.

Influence of Glucose Concentrations on Biofilm Formation, Motility, Exoprotease Production, and Quorum Sensing in Aeromonas hydrophila

2013 ◽  
Vol 76 (2) ◽  
pp. 239-247 ◽  
Author(s):  
IQBAL KABIR JAHID ◽  
NA-YOUNG LEE ◽  
ANNA KIM ◽  
SANG-DO HA
Keyword(s):  
Quorum Sensing ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Homoserine Lactone ◽  
Protease Production ◽  
Acyl Homoserine Lactone ◽  
Human Pathogen ◽  
Initial Biofilm ◽  
Motility Inhibition

Aeromonas hydrophila recently has received increased attention because it is opportunistic and a primary human pathogen. A. hydrophila biofilm formation and its control are a major concern for food safety because biofilms are related to virulence. Therefore, we investigated biofilm formation, motility inhibition, quorum sensing, and exoprotease production of this opportunistic pathogen in response to various glucose concentrations from 0.05 to 2.5% (wt/vol). More than 0.05% glucose significantly impaired (P < 0.05) quorum sensing, biofilm formation, protease production, and swarming and swimming motility, whereas bacteria treated with 0.05% glucose had activity similar to that of the control (0% glucose). A stage shift biofilm assay revealed that the addition of glucose (2.5%) inhibited initial biofilm formation but not later stages. However, addition of quorum sensing molecules N-3-butanoyl-DL-homoserine lactone and N-3-hexanoyl homoserine lactone partially restored protease production, indicating that quorum sensing is controlled by glucose concentrations. Thus, glucose present in food or added as a preservative could regulate acyl-homoserine lactone quorum sensing molecules, which mediate biofilm formation and virulence in A. hydrophila.

scholarly journals Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

mBio ◽  
2017 ◽  
Vol 8 (3) ◽  
Author(s):  
Rita S. Valente ◽  
Pol Nadal-Jimenez ◽  
André F. P. Carvalho ◽  
Filipe J. D. Vieira ◽  
Karina B. Xavier
Keyword(s):  
Signal Transduction ◽  
Quorum Sensing ◽  
Plant Pathogen ◽  
Bacterial Species ◽  
Homoserine Lactone ◽  
Signal Molecules ◽  
Acyl Homoserine Lactone ◽  
Interspecies Interactions ◽  
Major Factors

ABSTRACT Bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. The diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. In the plant pathogen Pectobacterium wasabiae (formerly Erwinia carotovora), two signaling networks—the N-acyl homoserine lactone (AHL) quorum-sensing system and the Gac/Rsm signal transduction pathway—control the expression of secreted plant cell wall-degrading enzymes, its major virulence determinants. We show that the AHL system controls the Gac/Rsm system by affecting the expression of the regulatory RNA RsmB. This regulation is mediated by ExpR2, the quorum-sensing receptor that responds to the P. wasabiae cognate AHL but also to AHLs produced by other bacterial species. As a consequence, this level of regulation allows P. wasabiae to bypass the Gac-dependent regulation of RsmB in the presence of exogenous AHLs or AHL-producing bacteria. We provide in vivo evidence that this pivotal role of RsmB in signal transduction is important for the ability of P. wasabiae to induce virulence in response to other AHL-producing bacteria in multispecies plant lesions. Our results suggest that the signaling architecture in P. wasabiae was coopted to prime the bacteria to eavesdrop on other bacteria and quickly join the efforts of other species, which are already exploiting host resources. IMPORTANCE Quorum-sensing mechanisms enable bacteria to communicate through small signal molecules and coordinate group behaviors. Often, bacteria have various quorum-sensing receptors and integrate information with other signal transduction pathways, presumably allowing them to respond to different ecological contexts. The plant pathogen Pectobacterium wasabiae has two N-acyl homoserine lactone receptors with apparently the same regulatory functions. Our work revealed that the receptor with the broadest signal specificity is also responsible for establishing the link between the main signaling pathways regulating virulence in P. wasabiae. This link is essential to provide P. wasabiae with the ability to induce virulence earlier in response to higher densities of other bacterial species. We further present in vivo evidence that this novel regulatory link enables P. wasabiae to join related bacteria in the effort to degrade host tissue in multispecies plant lesions. Our work provides support for the hypothesis that interspecies interactions are among the major factors influencing the network architectures observed in bacterial quorum-sensing pathways. IMPORTANCE Quorum-sensing mechanisms enable bacteria to communicate through small signal molecules and coordinate group behaviors. Often, bacteria have various quorum-sensing receptors and integrate information with other signal transduction pathways, presumably allowing them to respond to different ecological contexts. The plant pathogen Pectobacterium wasabiae has two N-acyl homoserine lactone receptors with apparently the same regulatory functions. Our work revealed that the receptor with the broadest signal specificity is also responsible for establishing the link between the main signaling pathways regulating virulence in P. wasabiae. This link is essential to provide P. wasabiae with the ability to induce virulence earlier in response to higher densities of other bacterial species. We further present in vivo evidence that this novel regulatory link enables P. wasabiae to join related bacteria in the effort to degrade host tissue in multispecies plant lesions. Our work provides support for the hypothesis that interspecies interactions are among the major factors influencing the network architectures observed in bacterial quorum-sensing pathways.

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