scholarly journals Metabolic cross-feeding allows a gut microbial community to overcome detrimental diets and alter host behaviour

2019 ◽  
Author(s):  
Sílvia F. Henriques ◽  
Lúcia Serra ◽  
Ana Patrícia Francisco ◽  
Zita Carvalho-Santos ◽  
Célia Baltazar ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Interdisciplinary Approach ◽  
Commensal Bacteria ◽  
Constant Flow ◽  
Host Interactions ◽  
Metabolic Interactions ◽  
Necessary And Sufficient ◽  
Host Behaviour ◽  
Gut Microbial Community ◽  
The Impact

The impact of commensal bacteria on the host arises from complex microbial-diet-host interactions. Mapping metabolic interactions in gut microbial communities is therefore key to understand how the microbiome influences the host. Here we use an interdisciplinary approach including isotope-resolved metabolomics to show that in Drosophila melanogaster, Aceto-bacter pomorum (Ap) and Lactobacillus plantarum (Lp) establish a syntrophic relationship to overcome detrimental host diets and identify Ap as the bacterium altering the host’s feeding decisions. Specifically, we show that Lp generates lactate which is used by Ap to produce and provide amino acids that are essential to Lp allowing it to grow in imbalanced diets. Lactate is also necessary and sufficient for Ap to alter the fly’s protein appetite. Our data show that gut bacterial communities use metabolic interactions to become resilient to detrimental host diets and to ensure the constant flow of metabolites used by effector bacteria to alter host behaviour.

scholarly journals The Impact of Metabolic Scion–Rootstock Interactions in Different Grapevine Tissues and Phloem Exudates

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 349
Author(s):  
Sara Tedesco ◽  
Alexander Erban ◽  
Saurabh Gupta ◽  
Joachim Kopka ◽  
Pedro Fevereiro ◽  
...  
Keyword(s):  
Agronomic Traits ◽  
Phloem Exudate ◽  
Graft Union ◽  
Reciprocal Effect ◽  
Metabolic Markers ◽  
Interaction Studies ◽  
Metabolic Interactions ◽  
A Minor ◽  
The Impact ◽  
Related Compounds

In viticulture, grafting is used to propagate Phylloxera-susceptible European grapevines, thereby using resistant American rootstocks. Although scion–rootstock reciprocal signaling is essential for the formation of a proper vascular union and for coordinated growth, our knowledge of graft partner interactions is very limited. In order to elucidate the scale and the content of scion–rootstock metabolic interactions, we profiled the metabolome of eleven graft combination in leaves, stems, and phloem exudate from both above and below the graft union 5–6 months after grafting. We compared the metabolome of scions vs. rootstocks of homografts vs. heterografts and investigated the reciprocal effect of the rootstock on the scion metabolome. This approach revealed that (1) grafting has a minor impact on the metabolome of grafted grapevines when tissues and genotypes were compared, (2) heterografting affects rootstocks more than scions, (3) the presence of a heterologous grafting partner increases defense-related compounds in both scion and rootstocks in shorter and longer distances from the graft, and (4) leaves were revealed as the best tissue to search for grafting-related metabolic markers. These results will provide a valuable metabolomics resource for scion–rootstock interaction studies and will facilitate future efforts on the identification of metabolic markers for important agronomic traits in grafted grapevines.

Population dynamics driven by truncated stable processes with Markovian switching

2021 ◽  
Vol 58 (2) ◽  
pp. 505-522
Author(s):  
Zhenzhong Zhang ◽  
Jinying Tong ◽  
Qingting Meng ◽  
You Liang
Keyword(s):  
Population Dynamics ◽  
Sufficient Conditions ◽  
Necessary And Sufficient Conditions ◽  
Markovian Switching ◽  
Stable Processes ◽  
Necessary And Sufficient ◽  
The Impact

AbstractWe focus on the population dynamics driven by two classes of truncated $\alpha$-stable processes with Markovian switching. Almost necessary and sufficient conditions for the ergodicity of the proposed models are provided. Also, these results illustrate the impact on ergodicity and extinct conditions as the parameter $\alpha$ tends to 2.

scholarly journals Silencing and Oblivion of Psychological Trauma, Its Unconscious Aspects, and Their Impact on the Inflation of Vajrayāna. An Analysis of Cross-Group Dynamics and Recent Developments in Buddhist Groups Based on Qualitative Data

Religions ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 622 ◽  
Author(s):  
Anders
Keyword(s):  
Group Dynamics ◽  
Interdisciplinary Approach ◽  
Psychological Trauma ◽  
Ongoing Research ◽  
Psychological Mechanisms ◽  
Teacher Student ◽  
New Concepts ◽  
Recent Developments ◽  
Student Relationship ◽  
The Impact

The commercialization of Buddhist philosophy has led to decontextualization and indoctrinating issues across groups, as well as abuse and trauma in that context. Methodologically, from an interdisciplinary approach, based on the current situation in international Buddhist groups and citations of victims from the ongoing research, the psychological mechanisms of rationalizing and silencing trauma were analyzed. The results show how supposedly Buddhist terminology and concepts are used to rationalize and justify economic, psychological and physical abuse. This is discussed against the background of psychological mechanisms of silencing trauma and the impact of ignoring the unconscious in that particular context. Inadequate consideration regarding the teacher–student relationship, combined with an unreflective use of Tibetan honorary titles and distorted conceptualizations of methods, such as the constant merging prescribed in so-called 'guru yoga', resulted in giving up self-responsibility and enhanced dependency. These new concepts, commercialized as 'karma purification' and 'pure view', have served to rationalize and conceal abuse, as well as to isolate the victims. Therefore, we are facing societal challenges, in terms of providing health and economic care to the victims and implementing preventive measures. This use of language also impacts on scientific discourse and Vajrayāna itself, and will affect many future generations.

scholarly journals Impact of Nosema Disease and American Foulbrood on Gut Bacterial Communities of Honeybees Apis mellifera

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 525
Author(s):  
Poonnawat Panjad ◽  
Rujipas Yongsawas ◽  
Chainarong Sinpoo ◽  
Chonthicha Pakwan ◽  
Phakamas Subta ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Bacterial Communities ◽  
454 Pyrosequencing ◽  
Gut Bacteria ◽  
Nosema Ceranae ◽  
Paenibacillus Larvae ◽  
American Foulbrood ◽  
Nosema Disease ◽  
The Impact ◽  
Pathogenic Infection

Honeybees, Apis mellifera, are important pollinators of many economically important crops. However, one of the reasons for their decline is pathogenic infection. Nosema disease and American foulbrood (AFB) disease are the most common bee pathogens that propagate in the gut of honeybees. This study investigated the impact of gut-propagating pathogens, including Nosema ceranae and Paenibacillus larvae, on bacterial communities in the gut of A. mellifera using 454-pyrosequencing. Pyrosequencing results showed that N. ceranae was implicated in the elimination of Serratia and the dramatic increase in Snodgrassella and Bartonella in adult bees’ guts, while bacterial communities of P. larvae-infected larvae were not affected by the infection. The results indicated that only N. ceranae had an impact on some core bacteria in the gut of A. mellifera through increasing core gut bacteria, therefore leading to the induction of dysbiosis in the bees’ gut.

scholarly journals Impact of Intensive Land-Based Fish Culture in Qingdao, China, on the Bacterial Communities in Surrounding Marine Waters and Sediments

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Qiufen Li ◽  
Yan Zhang ◽  
David Juck ◽  
Nathalie Fortin ◽  
Charles W. Greer
Keyword(s):  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Fish Culture ◽  
Fish Ponds ◽  
Marine Area ◽  
Gradient Gel Electrophoresis ◽  
Reducing Bacteria ◽  
The Impact ◽  
Nitrate Reducing Bacteria

The impact of intensive land-based fish culture in Qingdao, China, on the bacterial communities in surrounding marine environment was analyzed. Culture-based studies showed that the highest counts of heterotrophic, ammonium-oxidizing, nitrifying, and nitrate-reducing bacteria were found in fish ponds and the effluent channel, with lower counts in the adjacent marine area and the lowest counts in the samples taken from 500 m off the effluent channel. Denaturing gradient gel electrophoresis (DGGE) analysis was used to assess total bacterial diversity. Fewer bands were observed from the samples taken from near the effluent channel compared with more distant sediment samples, suggesting that excess nutrients from the aquaculture facility may be reducing the diversity of bacterial communities in nearby sediments. Phylogenetic analysis of the sequenced DGGE bands indicated that the bacteria community of fish-culture-associated environments was mainly composed of Flavobacteriaceae, gamma- and deltaproteobacteria, including generaGelidibacter, Psychroserpen, Lacinutrix,andCroceimarina.

scholarly journals Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing

2020 ◽  
Vol 8 (12) ◽  
pp. 1977
Author(s):  
Søren Iversen ◽  
Thor Bech Johannesen ◽  
Anna Cäcilia Ingham ◽  
Sofie Marie Edslev ◽  
Staffan Tevell ◽  
...  
Keyword(s):  
16S Rrna ◽  
Bacterial Communities ◽  
Gene Sequencing ◽  
Alpha Diversity ◽  
Clinical Samples ◽  
Staphylococcal Species ◽  
Tuf Gene ◽  
Staphylococcus Hominis ◽  
The Impact ◽  
Arthroplasty Surgery

The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty to assess the impact of chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection of samples obtained from elective arthroplasty patients (n = 83) by swabbing anterior nares, skin sites in the groin and the site of planned surgery, before and after arthroplasty surgery, was analysed by 16S rRNA (V3-V4) gene sequencing and a complementary targeted tuf gene sequencing approach to comprehensively characterise alterations in staphylococcal communities. Significant reductions in alpha diversity was detected for both bacterial (p = 0.04) and staphylococcal (p = 0.03) groin communities after arthroplasty surgery with significant reductions in relative Corynebacterium (p = 0.001) abundance and Staphylococcus hominis (p = 0.01) relative staphylococcal abundance. In nares, significant reductions occurred for Staphylococcus hominis (p = 0.02), Staphylococcus haemolyticus (p = 0.02), and Staphylococcus pasteuri (p = 0.003) relative to other staphylococci. Staphylococcus aureus colonised 35% of anterior nares before and 26% after arthroplasty surgery. Staphylococcus epidermidis was the most abundant staphylococcal species at all sampling sites. No bacterial genus or staphylococcal species increased significantly after arthroplasty surgery. Application of a targeted tuf gene sequencing approach provided auxiliary staphylococcal community profiles and allowed species-level characterisation directly from low biomass clinical samples.

scholarly journals Threat and fear: Pragmatic purposes of emotionalisation in media discourse

2021 ◽  
Vol 25 (3) ◽  
pp. 746-766
Author(s):  
Vladimir I. Ozymenko ◽  
Tatiana V. Larina
Keyword(s):  
Critical Discourse Analysis ◽  
Interdisciplinary Approach ◽  
First Century ◽  
Media Discourse ◽  
Basic Emotions ◽  
Political Processes ◽  
Political Studies ◽  
American Media ◽  
Anglo American ◽  
The Impact

The impact of mass media on individuals and society is to a great extent based on emotions. We concentrate on fear as it is one of the basic emotions triggered by risk and threat, which is claimed to play a key role in the twenty-first century consciousness (Furedi 20018). The study focuses on the emotionalisation of fear in contemporary media discourse about Russia, more specifically, on constructions of Russian threat and fear of Russia in Anglo-American media texts to highlight pragmatic effects and to speculate on possible purposes of such discourses. The study aims to explore the functioning of the lexemes threat and fear , in textual contexts with the focus on their pragma-discursive characteristics. It identifies the mechanisms as well as linguistic tools involved in media strategies of scare-mongering. The dataset was derived from quality British and American newspapers in the period 2018-2020, and was analysed drawing on an interdisciplinary approach combining critical discourse analysis, pragmatics, medialinguistics, psycholinguistics and the theory of proximisation. The paper argues that appealing to emotions as well as constructing emotions is aimed at enhancing the persuasive function of media and fulfilling their own agenda. The persistent use of the words threat and fear in relation to Russia as well as the obsessive discussion of this topic in media aim to shape a certain negative public opinion of Russia among readerships. The findings show that to achieve this goal different strategies and linguistic tools are used including: exaggeration, repetition, proximisation, interrogative headlines, presupposition, among others. The results go beyond linguistics, and may find implementation in political studies, since they provide researchers with tools for understanding contemporary social and political processes.

scholarly journals New institutional challenges to the educational space: security as a criterion for development in the conditions of digitalization

2020 ◽  
pp. 81-93
Author(s):  
Sergei Vladimirovich Rabkin
Keyword(s):  
Social Development ◽  
National Security ◽  
Social Relations ◽  
Digital Technologies ◽  
Interdisciplinary Approach ◽  
The State ◽  
Internal Factors ◽  
Sociocultural Processes ◽  
Institutional Challenges ◽  
The Impact

The subject of this research is the institutional transformations of educational space associated with the global processes of digitalization of social relations, as well as internal factors in cooperation between society and the state with regards to determination of the criteria of security for their development. Using the general methodology of institutional analysis, methods of analogy and summary, as well as interdisciplinary approach towards studying the educational space, the author considers the questions of implementation of digital education technologies under the conditions of current sociocultural transformations. Special attention is turned to the balance between rational and immaterial factors that affect the formation of modern educational space, and thereby, specification of the new institutional challenges to the security of social development. The conducted analysis of the problems of implementation of such technologies in the system of higher education leads to the conclusion on the need for assessing the effectiveness of implementation of digital technologies in accordance with the institutional criteria that imply not only the impact of both, rational and immaterial factors of social development. The institutional nature of these factors is insufficiently studied, however determines the possibility of application of cognitive models in solution of the tasks aimed at ensuring national security in the conditions of digitalization of sociocultural processes. Therefore, the crucial institutional criterion that defines the effectiveness of implementation of digital technologies in the educational space consists in its security in all regards. The proposed institutional-criteria approach allows the state and society to focus on ensuring security of the educational space in the context of solution of relevant tasks of ensuring national security of the Russian Federation.

scholarly journals Detection of A-to-I RNA Editing in SARS-COV-2

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 41
Author(s):  
Ernesto Picardi ◽  
Luigi Mansi ◽  
Graziano Pesole
Keyword(s):  
Rna Editing ◽  
Time Course ◽  
Vero Cells ◽  
Innate Immune ◽  
Therapeutic Interventions ◽  
Clinical Samples ◽  
Transcriptome Data ◽  
Host Interactions ◽  
Rnaseq Data ◽  
The Impact

ADAR1-mediated deamination of adenosines in long double-stranded RNAs plays an important role in modulating the innate immune response. However, recent investigations based on metatranscriptomic samples of COVID-19 patients and SARS-COV-2-infected Vero cells have recovered contrasting findings. Using RNAseq data from time course experiments of infected human cell lines and transcriptome data from Vero cells and clinical samples, we prove that A-to-G changes observed in SARS-COV-2 genomes represent genuine RNA editing events, likely mediated by ADAR1. While the A-to-I editing rate is generally low, changes are distributed along the entire viral genome, are overrepresented in exonic regions, and are (in the majority of cases) nonsynonymous. The impact of RNA editing on virus–host interactions could be relevant to identify potential targets for therapeutic interventions.

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