Purification of Labeled Oligonucleotides by Precipitation with Ethanol

2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.prot100701
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Complete Removal ◽  
Differential Precipitation

If labeled oligonucleotides are to be used only as probes in hybridization experiments, complete removal of unincorporated label is generally not necessary. However, to reduce background to a minimum, the bulk of the unincorporated label should be separated from the labeled oligonucleotide. Most of the residual unincorporated precursors can be removed from the preparation by differential precipitation with ethanol, as described in this protocol, if the oligonucleotide is >18 nt in length.

Gum arabic helps prepare better lacey polymer films for specimen support in electron microscopy

1993 ◽  
Vol 51 ◽  
pp. 240-241
Author(s):  
Shailesh R. Sheth ◽  
Jayesh R. Bellare
Keyword(s):  
Electron Microscopy ◽  
Polymer Films ◽  
Gum Arabic ◽  
Complete Removal ◽  
Ammonium Bromide ◽  
Release Agent ◽  
Astigmatism Correction ◽  
Long Time ◽  
Micro Holes ◽  
Cetyl Trimethyl Ammonium

Specimen support and astigmatism correction in Electron Microscopy are at least two areas in which lacey polymer films find extensive applications. Although their preparation has been studied for a very long time, present techniques still suffer from incomplete release of the film from its substrate and presence of a large number of pseudo holes in the film. Our method ensures complete removal of the entire lacey film from the substrate and fewer pseudo holes by pre-treating the substrate with Gum Arabic, which acts as a film release agent.The method is based on the classical condensation technique for preparing lacey films which is essentially deposition of minute water or ice droplets on the substrate and laying the polymer film over it, so that micro holes are formed corresponding to the droplets. A microscope glass slide (the substrate) is immersed in 2.0% (w/v) aq. CTAB (cetyl trimethyl ammonium bromide)-0.22% (w/v) aq.

Suitability of forms of preparing seeds of Dalbergia miscolobium Benth for exposure to the tetrazolium test / Adequação das formas de preparação de sementes de Dalbergia miscolobium Benth para exposição ao teste de tetrazolium

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Juliana Martins de Mesquita Matos ◽  
Rosana De Carvalho Cristo Martins ◽  
Valéria Regina Bellotto ◽  
Lilian Gomes da Silva Rocha ◽  
Eloiza Aparecida Barbosa ◽  
...  
Keyword(s):  
Endangered Species ◽  
Filter Paper ◽  
Federal District ◽  
Complete Removal ◽  
Sensu Stricto ◽  
The Other ◽  
Distrito Federal ◽  
Viable Seeds ◽  
Range Test ◽  
Tetrazolium Test

Dalbergia miscolobium or Jacarandá do Cerrado is a species of legume in the Fabaceae family. It occurs in the sensu stricto Cerrado and in the dystrophic cerradão. It shows potential for landscaping and for recovering damaged areas. It is an endangered species and therefore is protected by the law that prevents cut in areas of the Federal District (Decree No. 14.783/93). The purpose of this study was to determine the best procedure to prepare seeds of Dalbergia miscolobium to assess viability in the tetrazolium test. We carried out the following treatments: i) hydration on filter paper at 25 ° C, ii) hydration on filter paper at 25 ° C followed by a cut in the tegument and iii) hydration on filter paper at 25 ° C followed bya complete removal of the tegument. The results were analyzed using analysis of variance and the Tukey range test. The analyzes showed that the best procedure to prepare seeds of Dalbergia miscolobium is the treatment in which there is a hydration followed by the complete removal of the integument. Where 78% of the seeds showed uniform staining, indicating that the seeds analyzed are of good quality. The other treatments, hydration and hydration followed by cutting, showed respectively 35% and 41% of viable seeds. RESUMO A Dalbergia miscolobium ou Jacarandá do Cerrado é uma espécie de leguminosa da família Fabaceae. Ocorre no sentido stricto Cerrado e no cerradão distrófico. Possui potencial para paisagismo e para recuperar áreas degradadas. É uma espécie ameaçada de extinção e, portanto, está protegida pela lei que previne o corte em áreas do Distrito Federal (Decreto 14.783 / 93). O objetivo deste estudo foi determinar o melhor procedimento de prepararação das sementes de Dalbergia miscolobium para serem submetidas à análise de viabilidade pelo teste de tetrazólio. Foram realizados os seguintes tratamentos: i) hidratação em papel de filtro a 25 ° C, ii) hidratação em papel de filtro a 25 ° C seguida de um corte no tegumento e iii) hidratação em papel de filtro a 25 ° C seguido de remoção completa do tegumento. Os resultados foram analisados utilizando-se a análise de variância e o teste de médias de Tukey. As análises mostraram que o melhor procedimento para preparar sementes de Dalbergia miscolobium é o tratamento em que há uma hidratação seguida pela remoção completa do tegumento, onde 78% das sementes apresentaram coloração uniforme, indicando que as sementes analisadas são de boa qualidade. Os demais tratamentos, hidratação e hidratação seguida de corte, mostraram respectivamente 35% e 41% de sementes viáveis.

Medulla oblongata cavernoma removal through a lazy far lateral approach: operative video and technical nuances

2019 ◽  
Vol 1 (2) ◽  
pp. V21
Author(s):  
Carlos Candanedo ◽  
Samuel Moscovici ◽  
Sergey Spektor
Keyword(s):  
Medulla Oblongata ◽  
Cavernous Malformation ◽  
Complete Removal ◽  
Lateral Approach ◽  
Neurological Deficits ◽  
Total Resection ◽  
Postoperative Mri ◽  
Far Lateral Approach ◽  
Far Lateral ◽  
Brainstem Cavernous Malformation

Removal of brainstem cavernous malformation remains a surgical challenge. We present a case of a 63-year-old female who was diagnosed with a large cavernoma located in the medulla oblongata. The patient suffered three episodes of brainstem bleeding resulting in significant neurological deficits (hemiparesis, dysphagia, and dysarthria). It was decided to remove the cavernoma through a left-sided modified far lateral approach.3The operative video demonstrates the surgical steps and nuances of a complete removal of this complex medulla oblongata cavernous malformation. Total resection was achieved without complications. Postoperative MRI revealed no signs of residual cavernoma with clinical improvement.The video can be found here: https://youtu.be/BTtMvvLMOFM.

Application of iron-coated sand on the treatment of toxic metals

2004 ◽  
Vol 4 (5-6) ◽  
pp. 335-341 ◽  
Author(s):  
Jae-Kyu Yang ◽  
Yoon-Young Chang ◽  
Sung-Il Lee ◽  
Hyung-Jin Choi ◽  
Seung-Mok Lee
Keyword(s):  
Heavy Metals ◽  
Selective Adsorption ◽  
Complete Removal ◽  
Batch Adsorption ◽  
Column Experiments ◽  
Adsorption Behaviour ◽  
Optimum Amount ◽  
Initial Ph ◽  
New Treatment ◽  
Coated Sand

Iron-coated sand (ICS) prepared by using FeCl3 and Joomoonjin sand widely used in Korea was used in this study. In batch adsorption kinetics, As(V) adsorption onto ICS was completed within 20 minutes, while adsorption of Pb(II), Cd(II), and Cu(II) onto ICS was slower than that of As(V) and strongly depended on initial pH. At pH 3.5, ICS showed a selective adsorption of Pb(II) compared to Cd( II) and Cu(II) . However, above pH 4.5, near complete removal of Pb(II), Cd(II), and Cu(II) was observed through adsorption or precipitation depending on pH. As(V) adsorption onto ICS occurred through an anionic-type and followed a Langmuir-type adsorption behaviour. In column experiments, pH was identified as an important parameter in the breakthrough of As(V). As(V) breakthrough at pH 4.5 was much slower than at pH 9 due to a strong chemical bonding between As(V) and ICS as similar with batch adsorption behaviour. With variation of ICS amounts, the optimum amount of ICS at pH 4.5 was identified as 5.0 grams in this research. At this condition, ICS could be used to treat 200 mg of As(V) with 1 kg of ICS until 50 ppb of As(V) appeared in the effluent. In this research, as a new treatment system, ICS can be potentially used to treat As(V) and cationic heavy metals.

Microbiological Performance of Multi-Stage Stabilization Ponds for Effluent Use in Agriculture

1991 ◽  
Vol 23 (7-9) ◽  
pp. 1517-1524 ◽  
Author(s):  
M. M. Saqqar ◽  
M. B. Pescod
Keyword(s):  
Environmental Parameters ◽  
Complete Removal ◽  
Faecal Coliform ◽  
Winter Period ◽  
Intestinal Nematode ◽  
Stabilization Ponds ◽  
Multi Stage ◽  
Guideline Value ◽  
Temperature Dependent Model ◽  
Egg Removal

The paper presents data on total and faecal coliform removal and on intestinal nematode egg removal achieved in the Al-Samra stabilization ponds in Jordan during the winter period December 1986 to March 1987. A total retention of 34 days in the series of 10 ponds was able to achieve complete removal of nematode eggs at the pond temperature 12-15°C but was not able to meet the WHO guideline value for faecal conforms of ≤ 1000/100 ml. In addition to retention time having a positive effect on faecal conform die-off, it was shown that surface organic loading, pond pH, BOD5 concentration, and depth, influenced the rate of faecal coliform die-off. It became apparent that interactions existed among the operational and environmental parameters but that the simple time-and-temperature-dependent model for faecal conform die-off was no longer appropriate.

New Process for Physico-Chemical Pretreatment of Dairy Effluents with Agricultural Use of Sludge Produced

1990 ◽  
Vol 22 (9) ◽  
pp. 93-100 ◽  
Author(s):  
Á Kárpáti ◽  
L. Bencze ◽  
J. Borszéki
Keyword(s):  
Field Experiments ◽  
Complete Removal ◽  
Grain Production ◽  
Molecular Weights ◽  
Physico Chemical ◽  
New Process ◽  
Agricultural Use ◽  
Removal Of Fat ◽  
Biological Methods ◽  
Dairy Effluents

A combined physico-chemical process is suitable for the effective pretreatment of dairy effluents, achieving almost complete removal of fat, and considerable reduction of protein content. The specific cost of COD removal using the process is only half or a third of that using aerobic biological methods. Depending on the chemicals used, further treatment or, in certain cases, agricultural utilization, of the sludge is possible. In addition, the components remaining in the effluent have low molecular weights, are easily degradable, and are very favourable as nutrients in municipal aerobic post-treatment. Investigation of the sludge showed that it did not contain any pathogen organisms and therefore its application on land is recommended. Laboratory and field experiments have shown that, with agricultural use of the sludge, increased plant growth and grain production were achieved.

The Use of Ultrasound in Bio-Treatment of Jute Yarn with Laccase Enzyme

2019 ◽  
Vol 12 (4) ◽  
pp. 275-286
Author(s):  
Ali A. Zolriasatein
Keyword(s):  
Tensile Strength ◽  
Reaction Times ◽  
Complete Removal ◽  
Jute Fiber ◽  
Light Fastness ◽  
Color Strength ◽  
Yellowness Index ◽  
Laccase Enzyme ◽  
Enzymatic Delignification ◽  
Jute Yarn

Background and Objective: Jute fiber is highly sensitive to the action of light. Significant features of the photochemical changes lose its tensile strength and develop a yellow color. It has been proved that the phenolic structure of lignin is responsible for the yellowing of jute fiber. In order to remove lignin, jute yarns were treated with laccase enzyme in different treatment times and ultrasonic powers. Lower whiteness index and higher yellowness index values were obtained by the laccase-ultrasound system in contrast to conventional laccase treatment. Methods: The laccase enzyme which entered the fibers by applying ultrasound, decreased the tensile strength while the loss in tensile strength was lower at high ultrasound intensities. FT-IR spectrum showed that the band at 1634 cm-1 assigned to lignin completely disappeared after laccase treatment in the presence of ultrasound. The absence of this peak in the laccase-ultrasound treated jute yarn suggests complete removal of lignin. Change in the morphology of fibers was observed by SEM before and after enzymatic delignification. The laccase-ultrasound treated yarns showed a rougher surface and more porosity. On the other hand, it was more effective in fibrillation of the jute fibers than the conventional method. Finally, bio-treated jute yarns were dyed with basic and reactive dyes. Results: The results indicated that at low intensities of ultrasound and relatively long reaction times, lignin can be more effectively removed and dye strength (K/S) increased to a higher extent. Laccase-ultrasound treatment increased the color strength by 33.65% and 23.40% for reactive and basic dyes respectively. Conclusion: In the case of light fastness, the conventional laccase treated yarns provided better protection than laccase-ultrasound treated yarns.

Appendectomy in retrograde order for complete removal of endometriosis

2009 ◽  
pp. 1-4
Author(s):  
Myong Cheol Lim ◽  
Yong Jung Song ◽  
Dong Ock Lee ◽  
Dae Chul Jung ◽  
Chong-Woo Yoo ◽  
...  
Keyword(s):  
Complete Removal
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