The Effectiveness of Red Spinach Extract Ointment (Amaranthus tricolor L.) against Healing Second Degree Burns in Wistar Rat

2021 ◽  
Author(s):  
Tri Oktarina Safitri ◽  
Ali Napiah Nasution ◽  
Chrismis Novalinda Ginting ◽  
Ermi Girsang
Keyword(s):  
Wistar Rat ◽  
Amaranthus Tricolor ◽  
Spinach Extract ◽  
Second Degree

scholarly journals Effects of Lactobacillus rhamnosus (ATCC 7469) ointment on second-degree burn wound in Wistar rat

2018 ◽  
Vol 5 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Amir Abbas Barzegari ◽  
Masood Hashemzaei ◽  
Ali-Reza Alihemmati ◽  
Soroush Soltani ◽  
Behrouz Naseri ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Wistar Rat ◽  
Burn Wound ◽  
Degree Burn ◽  
Second Degree

scholarly journals Therapeutic Effect of Red Spinach (Amaranthus tricolor L.) Extract on Pancreatic MDA Levels Rats (Rattus norvegicus) Exposed to MLD-STZ

2021 ◽  
Vol 7 (3) ◽  
pp. 129-133
Author(s):  
Kartika Rahma ◽  
Ois Nurcahyanti
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Rattus Norvegicus ◽  
Amaranthus Tricolor ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Spinach Extract ◽  
Sugar Levels

Background: Several studies have reported that an increase in blood glucose in patients with diabetes mellitus (DM) can cause complications to death that occur due to oxidative stress conditions due to excessive ROS production. Red spinach extract was found to be rich in antioxidant compounds. However, there has been no further research on the ability of red spinach to lower blood glucose levels and prevent oxidative stress, which can be seen from the levels of malondialdehyde (MDA; a marker of oxidative stress) in the DM body.Objective: This study aims to determine whether there is a change in blood glucose levels and MDA levels in DM animal models between the groups that were given red spinach extract and not.Methods: This study used white rats (Rattus norvegicus) which were divided into 5 groups: K(-) group in which the rats were not induced by diabetogenic agent and were not treated; K(+) group, the rats were made DM induced by multiple low dose streptozotocin (MLD-STZ); and T1, T2, and T3 groups were exposed to MLD-STZ and treated with red spinach extract (Amaranthus tricolor L.) at a dose of 200 mg/kgBW, 300 mg/kgBW and 400 mg/kgBW, respectively. Blood sugar levels were checked before and after treatment with extract using glucometer digital. Meanwhile, MDA levels were measured by TBA test using protein isolates from the pancreas of each rat.Results: The results showed that the average MDA levels in the K-, K+, T1, T2, and T3 groups were 1.759±0.08, 2.280±0.15, 2.303±0.11, 1.927±0.06, and 1.801±0.04. While the average blood sugar levels in the K-, K+, T1, T2, and T3 groups were 114.4±8.82, 464±72.78, 421.2±37.60, 140.6±20.19, and 176±13.06.Conclusion: It can be concluded that the administration of red spinach extract therapy in DM model rats was able to reduce both glucose levels and MDA levels. By lowering the levels of MDA compounds, it is also believed to be able to prevent oxidative stress in cells which causes tissue damage.

scholarly journals EFFECT OF CONSUMING RED SPINACH (AMARANTHUS TRICOLOR L) EXTRACT ON HEMOGLOBIN LEVEL IN POSTPARTUM MOTHERS

2017 ◽  
Vol 3 (4) ◽  
pp. 432-437
Author(s):  
Resty Himma Muliani ◽  
Ariawan Soejoenoes ◽  
Titi Suherni ◽  
Soeharyo Hadisaputro ◽  
Imam Djamaluddin Mashoedi
Keyword(s):  
Community Health Center ◽  
Heme Iron ◽  
Hemoglobin Level ◽  
Control Group ◽  
P Value ◽  
Amaranthus Tricolor ◽  
Control Group Design ◽  
Spinach Extract ◽  
Agriculture And Food ◽  
Postpartum Mothers

Background: Postpartum mothers are susceptible to anemia, due to loss of blood count during labor. Postpartum anemia causes uterine subinvolution which causes postpartum hemorrhage, facilitates puerperium infection, decreases breastfeeding and easy mamae infection. One alternative to prevent anemia in postpartum is to consume vegetables with high iron one red spinach. Red spinach is one of the non-heme iron sources needed to synthesize hemoglobin.Objective: To determine the effect of red spinach in increasing the levels of hemoglobin in postpastum mothers.Methods: This was a quasy experiment with pretest posttest with control group design in October-December 2017 at the Community Health Center of PONED Tarub, Tegal regency. Thirty postpartum mothers were included using purposive sampling, which 15 assigned in each group. Blood sampling and examination were performed in each respondent. Paired and Independent t-test were done for data analysis.Results: The results of the 14-day intervention of given red spinach extract showed that there was a significant increase of hemoglobin level (1.25 g / dL) with p-value 0.047.Conclusion: There is a significant effect of consuming red spinach extract (amaranthus tricolor L.) on the increase of  hemoglobin level in postpartum mother. It is recommended that midwives could apply this intervention and cooperate with agriculture and food processing in order to produce red spinach extract that increases hemoglobin, hematocrit and erythrocytes levels.

Surface epithelial ultrastructure of the polycystic ovary in the BB wistar rat

1986 ◽  
Vol 44 ◽  
pp. 252-253
Author(s):  
R. C. Kaufmann ◽  
F. K. Khosho ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Wistar Rat ◽  
Polycystic Ovaries ◽  
Chemical Diabetes ◽  
Scanning Electron

Diabetes decreases the fertility of females, but the mechanisms are not completely understood. In our investigations, we have found that 13% of the female BB Wistar rats that spontaneously developed chemical diabetes had persistent estrous. In this study the ovaries of these rats were examined by scanning electron microscopy(SEM) and compared to normal-cycling controls as well as to rats that had developed polycystic ovaries(PCO) by exposure to constant 1ight.

Ultrastructural and immunocytochemical studies of the rat hypothalamo-neurohypophysial system processed by rapid freezing and freeze-substitution fixation

1990 ◽  
Vol 48 (3) ◽  
pp. 884-885
Author(s):  
Seiji Shioda ◽  
Yasumitsu Nakai ◽  
Atsushi Ichikawa ◽  
Hidehiko Ochiai ◽  
Nobuko Naito
Keyword(s):  
Osmium Tetroxide ◽  
Freeze Substitution ◽  
Ultrastructural Localization ◽  
Wistar Rat ◽  
Neurosecretory Cells ◽  
Rapid Freezing ◽  
Sodium Metaperiodate ◽  
Tissue Sections ◽  
Ultrathin Sections ◽  
Electron Microscopes

The ultrastructure of neurosecretory cells and glia cells in the supraoptic nucleus (SON) of the hypothalamus and the neurohypophysis (PN) was studied after rapid freezing followed by substituion fixation. Also, the ultrastructural localization of vasopressin (VP) or its carrier protein neurophys in II (NPII) in the SON and PN was demonstrated by using a post-embedding immunoco1loidal gold staining method on the tissue sections processed by rapid freezing and freeze-substitution fixation.Adult male Wistar rat hypothalamus and pituitary gland were quenched by smashing against a copper block surface precooled with liquid helium and freeze-substituted in 3% osmium tetroxide-acetone solutions kept at -80°C for 36-48h. After substituion fixation, the tissue blocks were warmed up to room temperature, washed in acetone and then embedded in an Epon-Araldite mixture. Ultrathin sections mounted on 200 mesh nickel grids were immersed in saturated sodium metaperiodate and then incubated in each of the following solutions: 1 % egg albumin in phosphate buffer, VP or NPII (1/1000-1/5000) antiserum 24h at 4°C, 3) colloidal gold solution (1/20) 1h at 20°C. The sections were washed with distilled waterand dried, then stained with uranylacetate and lead citrate and examined with Hitachi HU-12A and H-800 electron microscopes.

Evaluation of the biochemical and anti-snake venom effects of Calliandra portoricensis extract fractions in wistar rat models

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
PE Ebong ◽  
HP Onyeama ◽  
MU Eteng ◽  
GO Igile ◽  
GE Egbung
Keyword(s):  
Snake Venom ◽  
Wistar Rat ◽  
Rat Models
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