A low-cost jitter separation and ADC spectral testing method without requiring coherent sampling

2018 ◽  
Author(s):  
Shravan K Chaganti ◽  
Li Xu ◽  
Degang Chen
Keyword(s):  
Low Cost ◽  
Testing Method ◽  
Spectral Testing

Study on Fruits Preservation in View of Surface Glossiness

2011 ◽  
Vol 338 ◽  
pp. 521-524
Author(s):  
Geng Jia Wang ◽  
Xin Guang Lv ◽  
Shu Ting Kuang ◽  
Hui Long Chen ◽  
Jing Peng
Keyword(s):  
Mathematical Model ◽  
Regression Analysis ◽  
Nondestructive Testing ◽  
Moisture Content ◽  
Linear Relation ◽  
Low Cost ◽  
Testing Method ◽  
Moisture Meter ◽  
R Value ◽  
Nondestructive Testing Method

Abstract: Nowadays, there is no low-cost fruit nondestructive testing method in China. The study aims to put forward a method about acquiring the moisture content of fruits indirectly by general photographic equipments. Firstly, general photographic equipment is used to obtain pictures of fruits or vegetables. Secondly, with the help of Matlab and Photoshop, the surface glossiness of the fruit or vegetable is gained. Thirdly, moisture content of a fruit or vegetable is measured through infrared moisture meter. Meanwhile, a Mathematical Model in Regression Analysis between the R value and the moisture content is established. Lastly, the non-linear relation between surface glossiness and moisture content of the fruit is found. Therefore, a new method about measuring the moisture content of fruits or vegetables through general photographic equipments is sought out.

Development CRISPR-Cas12-based rapid EGFR mutation detection kit.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e13669-e13669
Author(s):  
Kei Kunimasa ◽  
Yuya Bando ◽  
Miyako Shiraishi ◽  
Emi Aizawa ◽  
Kazumi Nishino ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Egfr Mutation ◽  
Low Cost ◽  
Lateral Flow ◽  
Detection Methods ◽  
Egfr Mutations ◽  
Driver Mutations ◽  
Guide Rna ◽  
Testing Method ◽  
Nsclc Patients

e13669 Background: Recently, several cancer gene panel tests have been developed to determine many driver mutations including lung cancer simultaneously. However, the current next-sequencing based platform is not ideal testing method in the clinical practice due to the high examination cost and the time required for the result to reach the field. By refining recently reported CRISPR-Cas-based nucleic acid detection methods, we developed a novel CRISPR-Cas12 family based rapid-detection kit for targetable EGFR mutations in non-small lung cancer (NSCLC) patients. Methods: To develop a powerful CRISPR-based testing method, we focused on unique Cas12 family proteins which contain a single RuvC nuclease domain that cleave target double-stranded DNA adjacent to protospacer adjacent motif (PAM) sequences as well as non-target single-stranded DNA (ssDNA) collaterally. We purified 6 Cas12 family proteins (Cas12a, Cas12c1, Cas12c2, Cas12g, Cas12i1, and Cas12i2) as well as two types of target-defined guide RNA for EGFR Ex.19 del(E746-A750) and L858R mutations. The target sequences including EGFR Ex.19(E746-A750) and L858R mutations were amplified from EGFR mutated cell lines, FFPE lung cancer tissues or cell free DNA (cfDNA) of NSCLC patients using PCR with PrimeSTAR GXL DNA polymerase (Takara, Shiga, Japan) or isothermal recombinase polymerase amplification (RPA) with TwistAmp Basic (TwistDx, Cambridge, UK). CRISPR-Cas12-based cleavage assay was conducted in a reaction buffer consisting of 10 nM Cas12 proteins, 10 nM purified guide RNA, 0.4nM target DNA, and 50 nM collateral ssDNA (quenched fluorescent DNA reporter) in a 20 μl volume at 37 °C for 1 h. The excited fluorescence, which is an indicator of the presence of target cancer mutation, was measured by Synergy H1 hybrid Multi-Mode Reader (BioTek, Vermont, USA). For simpler instrument-free and portable detection lateral flow readouts were also developed and tested using Milenia HybriDetect1 kit (TwistDx). Results: Cas12a, Cas12i1, Cas12i2-based assays successfully detected the EGFR Ex.19 del(E746-A750) and L858R mutations from PCR and RPA products. Lateral flow readout kit could also detect EGFR Ex.19 del (E746-A750) mutation from cfDNA derived from NSCLC patients. It took about 3 hours from plasma collection to DNA extraction, RPA, and measurement with the kit. Conclusions: CRISPR-Cas based lateral flow readout kit can detect EGFR mutation at an unprecedented pace with low cost. Our developed system will be an innovative testing tool for lung cancer patients with low cost, rapid, multiplexable, and noninvasive procedure.

Creep Prediction of a Printed Wiring Board For Separable Land Grid Array Connector

2004 ◽  
Vol 127 (2) ◽  
pp. 185-188
Author(s):  
J. Cepeda-Rizo ◽  
Hsien-Yang Yeh
Keyword(s):  
Creep Behavior ◽  
Low Cost ◽  
Polymeric Materials ◽  
Superposition Method ◽  
Printed Wiring Board ◽  
Three Point Bending ◽  
Testing Method ◽  
Temperature Superposition ◽  
Mechanical Fastening ◽  
Wiring Board

The paper presents a study of creep behavior of a printed wiring board caused by the mechanical fastening of separable connector, known as a land grid array. Time-temperature superposition method was employed to predict the lifetime creep behavior. A low-cost testing method based on the ASTM D790 three-point bending procedure was developed to predict and characterize creep of polymeric materials under low temperature, low stress, and large elapsed times.

A market testing method based on crowd funding

2017 ◽  
Vol 45 (4) ◽  
pp. 19-23 ◽  
Author(s):  
Robert Charles Sheldon ◽  
Martin Kupp
Keyword(s):  
Design Methodology ◽  
Low Cost ◽  
New Products ◽  
Content Type ◽  
Scarce Resources ◽  
Commercial Potential ◽  
Testing Method ◽  
Commercial Viability ◽  
Novel Method ◽  
Intuitive Method

Purpose This paper provides a tool for managers to overcome a common paradox in situations of innovation. The paradox occurs when the commercial viability of ideas for new products and services is unknown, making managers loath to invest scarce resources in developing them for fear that they will lose them if the idea is a non-starter. The result is that ideas sit on the shelf, idle and untested. This paper provides a low-cost, intuitive method for determining if a new idea has sufficient commercial potential to warrant investing in its development, thereby resolving the paradox. Design/methodology/approach The method is based on six marketing mechanisms derived from crowd funding: choosing a target prospect and positioning, creating a prototype, setting a price based on value, locating prospects; getting an address, communicating with prospects; delivering an effective pitch, and measuring results through advance commitments. The method is illustrated using two entrepreneurial cases. Findings Managers who use the method can validate ideas in the marketplace quickly and at low cost, obtaining the information they need to justify an investment in the research, planning and analysis required for commercialization. Originality/value The paper identifies a common but little discussed obstacle to innovation, the development paradox, and proposes a novel method of overcoming it. Market testing is not new, but most known methods do not solve the development paradox because they require a significant outlay of resources to undertake.

Probability models for detecting transgenic plants

2008 ◽  
Vol 18 (2) ◽  
pp. 77-89 ◽  
Author(s):  
Carlos M. Hernández-Suárez ◽  
Osval A. Montesinos-López ◽  
Graham McLaren ◽  
José Crossa
Keyword(s):  
Transgenic Plants ◽  
Sample Size ◽  
High Probability ◽  
Probabilistic Models ◽  
Negative Binomial ◽  
Low Cost ◽  
Group Testing ◽  
Probability Models ◽  
False Negatives ◽  
Testing Method

AbstractWhen detecting the adventitious presence of transgenic plants (AP), it is important to use an appropriate testing method in the laboratory. Dorfman's group testing method is effective for reducing the number of laboratory analyses, but does not consider the case where AP is diluted below the sensitivity of the analyses, which causes the rate of false negatives to increase. The objective of this study is to propose binomial and negative binomial probabilistic models for determining the required sample size (n), number of pools (g), and size of the pool (k) for detecting individuals possessing AP with a probability ≥ (1 − α) (for a small α) given: (1) pool size (k); (2) estimated proportion of individuals with AP in the population (p); (3) concentration of the trait of interest (AP) in individual seeds (w); and (4) detection limit of the test (c) (AP concentration in a pool below which it cannot be detected). The proposed models consider the different rates of false positives (δ) and false negatives (λ), and the assessment of consumer and producer risks. Results have shown that when using the negative binomial, a required sample size n can be determined that guarantees a high probability that m individuals or g pools containing AP will be found. The pools formed have an optimum size, such that one element with AP will be detected at a low cost. The negative binomial distribution should be used when it is known that the proportion of individuals with AP in the population is p < 0.1; thus, it is guaranteed that m individuals or g pools of individuals with AP will be detected with high probability.

scholarly journals Compressive strength values dispersion of side-mixed and ready-mixed concretes

2017 ◽  
Vol 10 (5) ◽  
pp. 972-984
Author(s):  
A. ALVES ◽  
F. SANTOS ◽  
A. DEOGENES ◽  
K. MOREIRA ◽  
E. MESQUITA
Keyword(s):  
Compressive Strength ◽  
Low Cost ◽  
Influence Factors ◽  
Structure Design ◽  
Design Parameters ◽  
Reinforced Concrete Structure ◽  
Average Strength ◽  
Testing Method ◽  
Concrete Mixtures ◽  
Concrete Quality

Abstract Compressive strength testing method has been widely used as indirect way for evaluate the concrete quality, due to it simplest execution, reliability of the results achieved and low cost of execution. Independently, if the concrete was side or ready-mixed, it is necessary to achieve the compressive strength requirements stated during structure design, essentially because this parameter allows the obtainment of the average strength of the reinforced concrete structure analyzed. Following, as for ready-mixed, as for side-mixed concretes, it is need to ensure the correspondence between the concrete design parameters and the concrete mixed, namely these parameters are summarized in terms of the compressive strength and homogeneity in the different phases of the construction. This way, the present work aims to do the analysis of the dispersions of the compressive strength of concretes mixtures (corresponding to compressive strength classes of 20, 25, 30, 35 and 40 MPa), and identify some influence factors, in this case, the influence of the workers team variation. Two mixtures (35 MPa and 40 MPa) were ready-mixed, while five of the concrete mixtures were side-mixed, by five different worker’s group with same time of experience on concrete’s manufacture. The results demonstrate that the variation of the workers team presents a high influence for the homogeneity of the compressive strength of the concrete.

A Travel Blog as a Space for Creation and Communication of Travel Models

2016 ◽  
Vol 40 (0) ◽  
pp. 119-0 ◽  
Author(s):  
Irina Morozova
Keyword(s):  
Content Analysis ◽  
Low Cost ◽  
Future Research ◽  
Testing Method ◽  
Wide Range ◽  
Definition Of ◽  
Travel Blog ◽  
Practical Implications ◽  
Selection Of

Purpose. The purpose of this article is to present the variety of travel models which are conveyed and promoted by amateur travel blogs. Methods. The research sample was constituted on the bases of selected Polish travel blogs which promote travel models. The basic criteria for the selection of these particular blogs was the representativeness and popularity among readers. The testing method was content analysis of selected blogs. Findings. The present study suggests a classification of travel blogs. The research hypothesis claiming that the authors of travel blogs publicize travel models was confirmed. Research and conclusions limitations. The study is focused only on amateur travel blogs which are written in Polish. During the process of research, the author focused on a range of topics of the posts as well as on the publication genres. The present study includes blogs about world travels, travelling with children as well asdogs and low-cost travels. Practical implications. The results of this study indicate a wide range of possible future research studies regarding travel blogs from different perspectives. Originality. This article attempts to establish the definition of a travel model and the main characteristics of a travel blogger which aspire to become a travelebrity. A classification of travel blogs using the 'travel model' key is also provided. Type of paper. The article presents the results of empirical research conducted by the author.

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