Control actuator vibration testing system is one of the key devices throughout the whole manufacturing line of missiles. To meet the requirements of fast acquirement real-time show, automatic test convenient for user to modify during the control actuator based on virtual instrument developing environment LabVIEW and PCI bus technology is built. Hardware composition, software development, as well as other various functions in some kind of control actuator vibration testing system, such as the analog signals generating, sampling, recurring,auto-detecting and etc., are discussed in this paper. Practices prove this system works well with stable performance, and meet the requirements of design.