A Hand-Held Indentation System for the Assessment of Mechanical Properties of Soft Tissues In Vivo

2009 ◽  
Vol 58 (9) ◽  
pp. 3079-3085 ◽  
Author(s):  
Min-Hua Lu ◽  
W. Yu ◽  
Qing-Hua Huang ◽  
Yan-Ping Huang ◽  
Yong-Ping Zheng
Keyword(s):  
Mechanical Properties ◽  
Soft Tissues

Reliability Investigation of Tissue Resonator Indenter Device

2010 ◽  
Author(s):  
Ming Jia ◽  
Jean W. Zu ◽  
Alireza Hariri
Keyword(s):  
Mechanical Properties ◽  
Soft Tissue ◽  
Soft Tissues ◽  
Tissue Properties ◽  
University Of Toronto ◽  
In Vivo Measurements ◽  
Disease Diagnostics ◽  
Working Principle ◽  
The University

Knowledge of tissue mechanical properties is widely required by medical applications, such as disease diagnostics, surgery operation, simulation, planning, and training. A new portable device, called Tissue Resonator Indenter Device (TRID), has been developed for measurement of regional viscoelastic properties of soft tissues at the Bio-instrument and Biomechanics Lab of the University of Toronto. As a device for soft tissue properties in-vivo measurements, the reliability of TRID is crucial. This paper presents TRID’s working principle and the experimental study of TRID’s reliability with respect to inter-reliability, intra-reliability, and the indenter misalignment effect as well. The experimental results show that TRID is a reliable device for in-vivo measurements of soft tissue mechanical properties.

Non-Invasive Measurement of In-Vivo Elasticity of Skeletal Muscles with MR-Elastography

2007 ◽  
Vol 342-343 ◽  
pp. 901-904
Author(s):  
Yu Bong Kang ◽  
T. Oida ◽  
Duk Young Jung ◽  
A. Fukuma ◽  
T. Azuma ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Shear Modulus ◽  
Skeletal Muscles ◽  
Soft Tissues ◽  
Viscosity Coefficient ◽  
Non Invasive ◽  
Novel Method ◽  
Human Calf ◽  
Invasive Measurement

In order to evaluate the mechanical properties of the human skeletal muscles, the elasticity and viscosity of the human calf muscles were measured with Magnetic Resonance Elastography (MRE). MRE is a novel method to measure the mechanical properties of living soft tissues in vivo quantitatively by observing the strain waves propagated in the object. In this study, the shear modulus and viscosity coefficient were measured with MRE. The shear modulus was 3.7 kPa in relaxed state, and increased with increasing the muscle forces. Interestingly, the viscosity was changed with the vibration frequency applied to the muscles, that was 4.5 Pa·s at 100Hz vibration and 2.4 Pa·s at 200Hz vibration. This shows clearly the visco-elastic property.

scholarly journals DISPOSABLE SYSTEM FOR IN-VIVO MECHANICAL CHARACTERIZATION OF SOFT TISSUES BASED ON VOLUME MEASUREMENT

2018 ◽  
Vol 18 (04) ◽  
pp. 1850037 ◽  
Author(s):  
SEYED ALI ELAHI ◽  
NATHANAEL CONNESSON ◽  
YOHAN PAYAN
Keyword(s):  
Mechanical Properties ◽  
Soft Tissues ◽  
Signal To Noise Ratio ◽  
Volume Measurement ◽  
Optical Measurements ◽  
Signal To Noise ◽  
Noise Ratio ◽  
Apex Height

In-vivo characterization of soft tissues is a key step toward biomechanical simulation and planning of intra-operative assisted surgery. To achieve this, aspiration method is a standard technique: tissue is aspirated through a hole while measuring the pressure and associated apex height. An inverse problem is then solved to identify the material mechanical properties. In the literature, the apex height is usually measured using a camera, which induces design difficulties, in particular in regards on the required sterilization process for in-vivo measurements. In this paper, the idea is to replace the apex height optical measurement by the measurement of the aspirated tissue volume. The proposed method enables to reduce the system head to a simple tube: sterilizations becomes easy and the system is disposable after use. The proposed system is thus the simplest, lightest and cheapest one could achieve. It is also to the authors knowledge the first time ever in aspiration method that the aspired volume is the extracted data. As the data signal-to-noise ratio is the main factor impacting any applied inverse method when extracting the mechanical properties, the aim of this work is to assess and compare the experimental signal-to-noise ratio in the raw volume measurements obtained either optically or with the method proposed. Explicit results of inverse methods using volumes as input data are not presented in this paper for concision purpose. The effects on accuracy of various experimental parameters has been investigated and quantified: the volume measurement has proved to present a same order or even better signal-to-noise ratio compared to optical measurements.

Mechanical Properties of the Epidermis and Stratum Corneum Determined by Submicron Indentation In Vitro

2009 ◽  
Author(s):  
Marion Geerligs ◽  
Lambert C. A. v. Breemen ◽  
Gerrit W. M. Peters ◽  
Paul A. J. Ackermans ◽  
Cees W. J. Oomens ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Stratum Corneum ◽  
Mechanical Behavior ◽  
Soft Tissues ◽  
Sensory Nerve ◽  
Complex Nature ◽  
Outer Skin ◽  
Viable Epidermis

The outer skin layers are important drug and vaccine delivery targets in the treatment of diseases. These skin layers possess some important characteristics making them favorable sites for pain-free delivery with minimal damage: a rich population of immunologically sensitive cells as well as the lack of blood vessels and sensory nerve endings [1]. Until today, however, the development of effective cell targeting methods is acquainted with many challenges. A collective shortcoming is a poor understanding of the key mechanical properties of the outer skin layers, e.g. the stratum corneum and epidermis. The anisotropic, dynamic and very complex nature of skin makes it difficult to perform proper mechanical testing as well as to obtain reliable, reproducible data. The stratum corneum is an effective physical barrier of dead cells with a “brick-and-mortar” structure, while the viable epidermis mainly consists of actively migrating keratinocytes constantly undergoing massive morphological and compositional changes. As a consequence, the structure differences among the skin layers lead to significant variations in mechanical properties. Since there is no method available to determine the mechanical behavior of isolated viable epidermis in vivo or in vitro, the mechanical behavior of epidermis and stratum corneum only are investigated here. A commercially available indentation system has been adapted to enable the measurement of these thin soft tissues in an in vitro set up. Combining the outcomes of the two skin layer types leads to an assessment of the contribution of the viable epidermis to the mechanical behavior of skin. To our knowledge, no data have been published yet regarding mechanical bulk properties of (viable) epidermis, while no consistency exists with respect to those of the stratum corneum.

Relating Network Topology to Network Mechanics

2012 ◽  
Author(s):  
Eric M. Christiansen ◽  
Mohammad F. Hadi ◽  
Victor H. Barocas
Keyword(s):  
Mechanical Properties ◽  
Network Topology ◽  
Soft Tissues ◽  
Topological Properties ◽  
Fiber Network ◽  
Fiber Networks ◽  
Protein Fiber ◽  
Network Topologies

Soft tissues are comprised of underlying fiber networks of collagen and other fibrous proteins and biopolymers. Thus, the ability to model the deformation of fiber networks is critical to understanding the mechanics of tissues in vivo and in vitro [1]. Complicating the issue, protein fiber networks are comprised of a range of different topologies that behave differently under load. There is a clear need for a method to derive network parameters that characterize the network and allow for the prediction of their behavior. In this study, we characterized several different random fiber network types based on their intrinsic mechanical and topological properties. Such characterization would improve our ability to select microscale network topologies that match the mechanical properties we observe in healthy and diseased native tissues [2]. It would also improve our ability to discern the outcome of microstructural changes in tissues (such as from remodeling or injury) on their overall mechanics.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

A Comparative Study of the in vivo Distribution of 32P-Polyphosphates and 32P-Orthophosphate

1972 ◽  
Vol 11 (01) ◽  
pp. 70-78
Author(s):  
Esther Miller ◽  
Leopoldo Anghileri
Keyword(s):  
Radiation Dose ◽  
Comparative Study ◽  
Soft Tissues ◽  
Tumor Bearing ◽  
In Vivo Distribution ◽  
The Cross ◽  
Total Body

SummaryThe distribution of 32P-polyphosphates (lineal and cross-linked) and 32Porthophosphate in normal and tumor bearing animals has been studied. Differences between the cross-linked and the lineal form are related to a different degree of susceptibility to the hydrolysis by the phosphatases. In contrast to orthophosphate, the polyphosphates showed a lower accumulation in soft tissues which gives an advantageous reduction of the total body radiation dose.

scholarly journals Ultra-strong bio-glue from genetically engineered polypeptides

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chao Ma ◽  
Jing Sun ◽  
Bo Li ◽  
Yang Feng ◽  
Yao Sun ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Covalent Bond ◽  
Genetically Engineered ◽  
Supramolecular Interactions ◽  
Molar Ratio ◽  
High Adhesion ◽  
Strong Adhesion ◽  
Order Of Magnitude

AbstractThe development of biomedical glues is an important, yet challenging task as seemingly mutually exclusive properties need to be combined in one material, i.e. strong adhesion and adaption to remodeling processes in healing tissue. Here, we report a biocompatible and biodegradable protein-based adhesive with high adhesion strengths. The maximum strength reaches 16.5 ± 2.2 MPa on hard substrates, which is comparable to that of commercial cyanoacrylate superglue and higher than other protein-based adhesives by at least one order of magnitude. Moreover, the strong adhesion on soft tissues qualifies the adhesive as biomedical glue outperforming some commercial products. Robust mechanical properties are realized without covalent bond formation during the adhesion process. A complex consisting of cationic supercharged polypeptides and anionic aromatic surfactants with lysine to surfactant molar ratio of 1:0.9 is driven by multiple supramolecular interactions enabling such strong adhesion. We demonstrate the glue’s robust performance in vitro and in vivo for cosmetic and hemostasis applications and accelerated wound healing by comparison to surgical wound closures.

scholarly journals Concomitant control of mechanical properties and degradation in resorbable elastomer-like materials using stereochemistry and stoichiometry for soft tissue engineering

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mary Beth Wandel ◽  
Craig A. Bell ◽  
Jiayi Yu ◽  
Maria C. Arno ◽  
Nathan Z. Dreger ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Soft Tissue ◽  
Tissue Regeneration ◽  
Biological Tissues ◽  
Soft Tissue Regeneration ◽  
Soft Tissue Engineering ◽  
Degradation Properties ◽  
Enhance Cell Adhesion

AbstractComplex biological tissues are highly viscoelastic and dynamic. Efforts to repair or replace cartilage, tendon, muscle, and vasculature using materials that facilitate repair and regeneration have been ongoing for decades. However, materials that possess the mechanical, chemical, and resorption characteristics necessary to recapitulate these tissues have been difficult to mimic using synthetic resorbable biomaterials. Herein, we report a series of resorbable elastomer-like materials that are compositionally identical and possess varying ratios of cis:trans double bonds in the backbone. These features afford concomitant control over the mechanical and surface eroding degradation properties of these materials. We show the materials can be functionalized post-polymerization with bioactive species and enhance cell adhesion. Furthermore, an in vivo rat model demonstrates that degradation and resorption are dependent on succinate stoichiometry in the elastomers and the results show limited inflammation highlighting their potential for use in soft tissue regeneration and drug delivery.

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