Isolation, molecular characterization and antimicrobial susceptibility of Aeromonas spp. obtained from Tiger Grouper ( Epinephelus fuscoguttatus ) and Marble Goby ( Oxyeleotris marmoratus ) fish in Sabah, Malaysia

2020 ◽  
Vol 51 (10) ◽  
pp. 3972-3982
Author(s):  
Tien‐Tien Vicky Lau ◽  
Suat‐Moi Puah ◽  
Ching‐Kee Karen Hon ◽  
Fui‐Fui Ching ◽  
Jin‐Ai Mary Anne Tan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Antimicrobial Susceptibility ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Oxyeleotris Marmoratus

scholarly journals Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon

2021 ◽  
Vol 9 (6) ◽  
pp. 1148
Author(s):  
Zahie Abboud ◽  
Lucia Galuppo ◽  
Marco Tolone ◽  
Maria Vitale ◽  
Roberto Puleio ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility ◽  
Meca Gene ◽  
Toxin Gene ◽  
Pcr Screening ◽  
E Coli ◽  
Presenting Symptoms ◽  
Flight Mass Spectrometry ◽  
Streptococcus Uberis

Mastitis is an infectious disease encountered in dairy animals worldwide that is currently a growing concern in Lebanon. This study aimed at investigating the etiology of the main mastitis-causing pathogens in Northern Lebanon, determining their antimicrobial susceptibility profiles, and identifying their antimicrobial resistance (AMR) genes. A total of 101 quarter milk samples were collected from 77 cows and 11 goats presenting symptoms of mastitis on 45 dairy farms. Bacterial identification was carried out through matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Antimicrobial susceptibility was tested by disc diffusion and broth microdilution methods. Molecular characterization included polymerase chain reaction (PCR) screening for genes encoding extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC among Enterobacterales isolates, and virulence factors among Staphylococcus isolates. Escherichia coli isolates were subjected to phylogenetic typing by a quadruplex PCR method. The most frequently identified species were Streptococcus uberis (19.2%), Streptococcus agalactiae (15.1%), E. coli (12.3%), and Staphylococcus aureus (10.96%). Gram-positive bacteria were resistant to macrolides and tetracycline, whereas gram-negative bacteria displayed resistance to ampicillin and tetracycline. Two ESBL genes, blaTEM (83.3%) and blaOXA (16.7%), and one AmpC beta-lactamase gene, blaCMY-II (16.7%), were detected among six E. coli isolates, which mainly belonged to phylogenetic group B1. Among Staphylococcus spp., the mecA gene was present in three isolates. Furthermore, four isolates contained at least one toxin gene, and all S. aureus isolates carried the ica operon. These findings revealed the alarming risk of AMR in the Lebanese dairy chain and the importance of monitoring antimicrobial usage.

Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans

Food Control ◽  
2016 ◽  
Vol 70 ◽  
pp. 96-102 ◽  
Author(s):  
Xudong Su ◽  
Jianmin Zhang ◽  
Weimin Shi ◽  
Xiaowei Yang ◽  
Yong Li ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility

scholarly journals ANALISIS KESESUAIAN PERAIRAN UNTUK BUDIDAYA IKAN KERAPU MACAN (Epinephelus fuscoguttatu) DI PERAIRAN PULAU TEGAL KECAMATAN TELUK PANDAN KABUPATEN PESAWARAN

2018 ◽  
Vol 6 (2) ◽  
pp. 705 ◽  
Author(s):  
Glenn Valentino ◽  
Abdullah Aman Damai ◽  
Herman Yulianto
Keyword(s):  
Water Quality ◽  
Secondary Data ◽  
Fish Farming ◽  
Primary Data ◽  
Marine Aquaculture ◽  
Quality Of Waters ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Physical And Chemical

Tiger grouper is a marine aquaculture commodities that has good prospects to be developed. One of the areas that could potentially be used as a place of tiger grouper fish farming is the Tegal Island, Pesawaran. The purpose of this research was to analyze the level of water quality for fish farming tiger grouper (Epinephelus fuscoguttatus). This research was carried out in March-May 2017 descriptive analytic nature by doing the observations on the quality of waters which include physical and chemical parameters. The data that used in this research sourced from primary data about the suitability waters of the island while the secondary data is form in research location maps. There were 4 sampling stations which determined with global positioning system (GPS). The results showed that the water quality which is owned by the island's waters on station 1, 2, 3 and 4 were in the quite appropriate category for the cultivation of tiger grouper.

Molecular characterization and antimicrobial susceptibility of Staphylococcus aureus from small-scale dairy systems in the highlands of Central México

2014 ◽  
Vol 95 (2) ◽  
pp. 181-196 ◽  
Author(s):  
T. B. Salgado-Ruiz ◽  
A. Rodríguez ◽  
D. Gutiérrez ◽  
B. Martínez ◽  
P. García ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility ◽  
Small Scale ◽  
Central Mexico ◽  
Dairy Systems ◽  
Small Scale Dairy

Hybridization of tiger grouper (Epinephelus fuscoguttatus ♀) x giant grouper (Epinephelus lanceolatus ♂) using cryopreserved sperm

Cryobiology ◽  
2020 ◽  
Vol 95 ◽  
pp. 84-89
Author(s):  
Bin Fan ◽  
Sen Yang ◽  
Le Wang ◽  
Xinghan Chen ◽  
Xiaochun Liu ◽  
...  
Keyword(s):  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Cryopreserved Sperm

scholarly journals LISTERIA MONOCYTOGENES IN OVINE SAUSAGE: MOLECULAR CHARACTERIZATION AND ANTIMICROBIAL SUSCEPTIBILITY OF ISOLATES

2011 ◽  
Vol 1 (1zero) ◽  
pp. 269
Author(s):  
M.N. Rosa ◽  
P. Mele ◽  
P. Soro ◽  
G. Coppa ◽  
G. Mula ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility

scholarly journals Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 37 ◽  
Author(s):  
Bo Wu ◽  
Qi Wang ◽  
Jie Cao ◽  
Jun Mei ◽  
Jing Xie
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Flesh Quality ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper

Transport in water is the most common method for achieving high survival rates when transporting cultured fish in China; yet, transport success relies on proper water quality and conditions. This research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (Epinephelus fuscoguttatus) during simulated transport. The transport water temperature for live tiger grouper was 15 °C, which had the highest survival rate, the lowest stress response, and metabolic rate, and this will reduce the susceptibility to diseases. It is stated that β-1,3-glucan influences the changes of cortisol content, heat shock protein 70, IL-1β, and IgM transcription levels during simulated transport. Rather than using ascorbic acid alone (the A-group), β-1,3-glucan (3.2 mg/L) in the presence of ascorbic acid (25 mg/L) can effectively reduce the increase of transport-induced serum cortisol content, heat shock protein 70, and IL-1β, but stimulated IgM. 25 mg/L ascorbic acid and 3.2 mg/L β-1,3-glucan had no obvious effect on the nutritional indexes and flavor of live tiger grouper; however, these can effectively reduce the stress response, improve the innate immune activity, and ensure a higher survival rate.

scholarly journals Campylobacter infections among Bulgarian children: molecular characterization and antimicrobial susceptibility

2020 ◽  
Vol 34 (1) ◽  
pp. 1038-1042
Author(s):  
Maria Pavlova ◽  
Ekaterina Alexandrova ◽  
George Donkov ◽  
Yordanka Mitova-Mineva ◽  
Todor Kantardjiev ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Antimicrobial Susceptibility ◽  
Campylobacter Infections
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