Effect of a denture base acrylic resin containing silver nanoparticles onCandida albicansadhesion and biofilm formation

Gerodontology ◽  
2014 ◽  
Vol 33 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Zhe Li ◽  
Jing Sun ◽  
Jing Lan ◽  
Qingguo Qi
2012 ◽  
Vol 112 (6) ◽  
pp. 1163-1172 ◽  
Author(s):  
A.F. Wady ◽  
A.L. Machado ◽  
V. Zucolotto ◽  
C.A. Zamperini ◽  
E. Berni ◽  
...  

2014 ◽  
Vol 905 ◽  
pp. 51-55 ◽  
Author(s):  
Sahana Bajracharya ◽  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon ◽  
Amornrat Wonglamsam ◽  
Theerathavaj Srithavaj

The aim of this study is to investigate the candidal biofilm formation on the silver nanoparticles (AgNPs) incorporated denture base heat-polymerized (poly methyl methacrylate), PMMA, resinand its flexural strength.Materials and methods:A total of 36PMMA resin specimens (15×15×2 mm3) were fabricated and divided into 4 groups based on their AgNPs contents (0%,0.5%, 1% and 1.5% w/w of polymer). The biofilm of clinical and reference strainsof C.albicanswere grown on PMMA resin specimens in the presence of yeast nitrogen base broth supplemented with 100 mM glucose at 37oC for 48 h and evaluated by tetrazolium reduction assay. The flexural strength of PMMA resin specimens (65×10×3.3 mm3) were tested by using three-point bending test. Data were analyzed by Kruskal-Wallis and Mann-Whitney U test at p<0.05. result:all="" 3="" groups="" with="" agnps="" showed="" significant="" reduction="" in="" biofilm="" formation="" of="" both="" strains="" i="">C.albicans compared to control (0% AgNPs) (p<0.05). Decreased flexural strength was observed with AgNPs groups compared with the control but within acceptable limit of ISO 20795-1. Conclusion:The reduction of the C. albicans biofilm was observed on the heat-polymerized PMMA resin incorporated with AgNPs. Regarding the flexural strength, the values were within ISO limit. These modified PMMA resin can be developed for the prevention or treatment of the candidal infection associated with the denture base material.


Author(s):  
Ali Mohammad Ali Aljafery ◽  
Aayad sayhood ◽  
Wasna'a M. Abdulridha ◽  
Ahmed Muhsin Yousif

2015 ◽  
Vol 09 (02) ◽  
pp. 207-212 ◽  
Author(s):  
Hamada Zaki Mahross ◽  
Kusai Baroudi

ABSTRACT Objective: The objective was to investigate the effect of silver nanoparticles (AgNPs) incorporation on viscoelastic properties of acrylic resin denture base material. Materials and Methods: A total of 20 specimens (60 × 10 × 2 mm) of heat cured acrylic resin were constructed and divided into four groups (five for each), according to the concentration of AgNPs (1%, 2%, and 5% vol.) which incorporated into the liquid of acrylic resin material and one group without additives (control group). The dynamic viscoelastic test for the test specimens was performed using the computerized material testing system. The resulting deflection curves were analyzed by material testing software NEXYGEN MT. Results: The 5% nanoparticles of silver (NAg) had significantly highest mean storage modulus E’ and loss tangent Tan δ values followed by 2% NAg (P < 0.05). For 1% nanosilver incorporation (group B), there were no statistically significant differences in storage modulus E’, lost modulus E” or loss tangent Tan δ with other groups (P > 0.05). Conclusion: The AgNPs incorporation within the acrylic denture base material can improve its viscoelastic properties.


2020 ◽  
Vol 14 (02) ◽  
pp. 268-273
Author(s):  
Sareh Habibzadeh ◽  
Arman Omidvaran ◽  
Solmauz Eskandarion ◽  
Ahmad Reza Shamshiri

Abstract Objectives This study aimed at assessing the effect of the addition of silver nanoparticles (SNPs) to a silicone soft liner on its tensile bond strength to denture base resin. Materials and Methods SNPs were added to Mucopren cold cure soft liner in 0 (control), 0.5, 1, 2, and 3 wt% concentrations and bonded in 120 stainless steel molds with processed heat cure acrylic resin blocks. Liner/resin combination samples were divided into two groups. The first half was stored for 2 days in distilled water at 37°C and then subjected to tensile bond strength, while the other half were thermocycled 3000 times before testing. Mean bond strength, expressed in mega pascals (MPa), was determined in the tensile test with the use of a universal testing machine at a crosshead speed of 5 mm/min. Statistical Analysis Data were analyzed using SPSS via one-way analysis of variance test, t-test, and Tukey’s posthoc, at a 95% confidence level (p < 0.05). Results Addition of SNPs and thermocycling both caused a significant reduction in the tensile bond strength of Mucopren to acrylic resin; however, in the thermocycled group, the bond strength increased with the increase in the concentration of SNPs (p < 0.001). Conclusion Addition of SNPs to Mucopren soft silicone liner reduces its tensile bond strength to denture acrylic resin.


2018 ◽  
Vol 21 (1) ◽  
pp. 87-91
Author(s):  
Wesam E Badr ◽  
Hassan M Sakr ◽  
Muhammad A Masoud ◽  
Mohamed A Quassem

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sirapat Pipattanachat ◽  
Jiaqian Qin ◽  
Dinesh Rokaya ◽  
Panida Thanyasrisung ◽  
Viritpon Srimaneepong

AbstractBiofilm formation on medical devices can induce complications. Graphene oxide/silver nanoparticles (GO/AgNPs) coated nickel-titanium (NiTi) alloy has been successfully produced. Therefore, the aim of this study was to determine the anti-bacterial and anti-biofilm effects of a GO/AgNPs coated NiTi alloy prepared by Electrophoretic deposition (EPD). GO/AgNPs were coated on NiTi alloy using various coating times. The surface characteristics of the coated NiTi alloy substrates were investigated and its anti-biofilm and anti-bacterial effect on Streptococcus mutans biofilm were determined by measuring the biofilm mass and the number of viable cells using a crystal violet assay and colony counting assay, respectively. The results showed that although the surface roughness increased in a coating time-dependent manner, there was no positive correlation between the surface roughness and the total biofilm mass. However, increased GO/AgNPs deposition produced by the increased coating time significantly reduced the number of viable bacteria in the biofilm (p < 0.05). Therefore, the GO/AgNPs on NiTi alloy have an antibacterial effect on the S. mutans biofilm. However, the increased surface roughness does not influence total biofilm mass formation (p = 0.993). Modifying the NiTi alloy surface using GO/AgNPs can be a promising coating to reduce the consequences of biofilm formation.


2012 ◽  
Vol 20 (6) ◽  
pp. 643-648 ◽  
Author(s):  
Juliê Marra ◽  
André Gustavo Paleari ◽  
Larissa Santana Rodriguez ◽  
Andressa Rosa Perin Leite ◽  
Ana Carolina Pero ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Helal F. Hetta ◽  
Israa M. S. Al-Kadmy ◽  
Saba Saadoon Khazaal ◽  
Suhad Abbas ◽  
Ahmed Suhail ◽  
...  

AbstractWe aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.


2021 ◽  
Vol 17 (1) ◽  
pp. 22-26
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
I Made Hendri Dwi Saputra ◽  

Introduction: The denture base is the part of the removable denture that is supported by good adaptation to the underlying oral tissue. Most of the denture bases are made of acrylic or polymethyl methacrylate resin, better known as PMMA. However, the acrylic resin also has disadvantages such as easily broken and absorbs liquids both water and chemicals. The chemical absorption like alcohol, ethanol, and some drinks that contain acidic materials will chemically be induced with acrylic resin and settle in the pores of the acrylic resin. The chemical damage or defect creates roughness on the surface of the acrylic resin which can cause cracking or crazing and a decrease in surface strength and hardness. Materials and Methods: The method used in this research was a laboratory experimental design with a post-test-only control group using 12 samples consisting of 2 different types of samples by measuring the surface roughness of the acrylic resin after immersing the sample with a predetermined time. Results and Discussions: One-way ANOVA test results showed a significant difference in surface roughness after the samples immersion with a value of p = 0.006 (p <0.05). Conclusions: Based on this research, can be concluded that there is an increase in the surface roughness of the heated polymerized acrylic resin for 3 hours and 4 hours of immersion. The longer the heated polymerized acrylic resin is soaked in arak hence the level of surface roughness increases.


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