scholarly journals Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation

2012 ◽  
Vol 20 (6) ◽  
pp. 643-648 ◽  
Author(s):  
Juliê Marra ◽  
André Gustavo Paleari ◽  
Larissa Santana Rodriguez ◽  
Andressa Rosa Perin Leite ◽  
Ana Carolina Pero ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Acrylic Resin

scholarly journals Antimicrobial and mechanical acrylic resin properties with silver particles obtained from Fusarium oxysporum

2018 ◽  
Vol 21 (1) ◽  
pp. 96 ◽  
Author(s):  
Tabata Prado Sato ◽  
Celio Ilidio Conjo ◽  
Rodnei Dennis Rossoni ◽  
Juliana Campos Junqueira ◽  
Renata Marques de Melo ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Fusarium Oxysporum ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Acrylic Resin ◽  
Control Group ◽  
Significant Difference ◽  
Color Alteration ◽  
Cie Lab

<p><strong>Objective: </strong>This study evaluated the effects of the incorporation of silver nanoparticles (AgNPs) obtained from <em>Fusarium oxysporum</em> on heat-activated acrylic resin (HAAR) and their influence on resin’s surface roughness, hardness, color alteration and antimicrobial capacity against <em>Candida albicans</em>. <strong>Material and Methods:</strong> For this, 50 discs of HAAR (2x5 mm) were produced and divided into three groups, Control: HAAR; Ag1: HAAR plus 0.539 mg of AgNPs; and Ag2: HAAR plus 1.1 mg of AgNPs. Knopp hardness (HK), surface roughness (Ra and Rz) and color alteration according to the CIE Lab were measured. Specimens were then evaluated <em>in vitro</em> with regard to <em>C. albicans</em> biofilm formation through formed colony count (CFU/mL). Scanning Electron Microscopy (SEM) and Atomic force microscopy (AFM) analyses were performed. <strong>Results:<em> </em></strong>The addition of AgNPs of both concentrations changed Ra, Rz and HK significantly. There was statistically significant difference for L (p=0.00); a*(p=0.00) and b*(p=0.00) parameters. There were no differences between Ag1 and Ag2 biofilm formation, but the comparison of both with the control group presented a significant reduction (p=0.0091) on biofilm formation. SEM and AFM images showed no signs of NPs clustering. <strong>Conclusion:</strong> It can be concluded tha AgNPs incorporation in HAAR was effective in reducing <em>C. albicans</em> activity, with a slight change in color and hardness of the material, being effective therefore, in regions such as the dental prostheses palate, which have lesser aesthetic appeal.</p><p><strong>Keywords</strong></p><p>Acrylic resins; Silver; Antimicrobial agents.</p>

scholarly journals Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles

2012 ◽  
Vol 112 (6) ◽  
pp. 1163-1172 ◽  
Author(s):  
A.F. Wady ◽  
A.L. Machado ◽  
V. Zucolotto ◽  
C.A. Zamperini ◽  
E. Berni ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Acrylic Resin ◽  
Denture Base

scholarly journals Eradication of a Mature Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilm From Acrylic Surfaces

2013 ◽  
Vol 24 (5) ◽  
pp. 487-491 ◽  
Author(s):  
Karen Tereza Altieri ◽  
Paula Volpato Sanitá ◽  
Ana Lucia Machado ◽  
Eunice Teresinha Giampaolo ◽  
Ana Cláudia Pavarina ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Microwave Irradiation ◽  
Sodium Hypochlorite ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Acrylic Resin ◽  
Positive Control ◽  
Chlorhexidine Gluconate ◽  
Control Group ◽  
Methicillin Resistant

Methicillin-resistant Staphylococcus aureus (MRSA) can grow as structured biofilm in different surfaces, including oral mucosa and denture surfaces. Such biofilms can be released into the oral fluids and aspirated, causing systemic infections such as aspiration pneumonia. This study evaluated the efficacy of two disinfectant solutions and microwave irradiation in disinfecting acrylic specimens contaminated with MRSA biofilm. Thirty-six acrylic specimens were made, sterilized and contaminated with MRSA (107 cfu/mL). After incubation (37 °C/48 h), the specimens were divided into 4 groups: not disinfected (positive control); soaking in 1% sodium hypochlorite for 10 min; soaking in 2% chlorhexidine gluconate for 10 min; and irradiating by microwave for 3 min at 650 W. The viability of cells was evaluated by XTT reduction method. All specimens from the positive control group showed biofilm formation after 48 h incubation. The mean absorbance value of the control specimens was 1.58 (OD at 492 nm). No evidence of biofilm formation was observed on specimens after the disinfection methods. Disinfection by soaking in 1% sodium hypochlorite and 2% chlorhexidine gluconate and irradiating by microwaves resulted in 100% reduction of MRSA biofilm metabolism. The use of chemical solutions and microwave irradiation was shown to be effective for eradicating mature MRSA biofilms on acrylic resin specimens.

scholarly journals Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

2012 ◽  
Vol 23 (5) ◽  
pp. 547-554 ◽  
Author(s):  
Delise Pellizzaro ◽  
Gregory Polyzois ◽  
Ana Lucia Machado ◽  
Eunice Teresinha Giampaolo ◽  
Paula Volpato Sanitá ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Biofilm Formation ◽  
Reduction Method ◽  
Acrylic Resin ◽  
Chlorhexidine Gluconate ◽  
Common Disease ◽  
Combined Method ◽  
Denture Stomatitis ◽  
Positive Controls

The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.

Effect of Diamond-Like Carbon Thin Film Coated Acrylic Resin on Candida albicans Biofilm Formation

2013 ◽  
Vol 22 (6) ◽  
pp. 451-455 ◽  
Author(s):  
José Renato Cavalcanti Queiroz ◽  
Sara Fernanda Fissmer ◽  
Cristiane Yumi Koga-Ito ◽  
Ana C. R. D. Salvia ◽  
Marcos Massi ◽  
...  
Keyword(s):  
Thin Film ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Acrylic Resin ◽  
Diamond Like Carbon ◽  
Carbon Thin Film

scholarly journals Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 425
Author(s):  
Ericles Otávio Santos ◽  
Pedro Lima Emmerich Oliveira ◽  
Thaís Pereira de Mello ◽  
André Luis Souza dos Santos ◽  
Carlos Nelson Elias ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Biofilm Formation ◽  
Acrylic Resin ◽  
Surface Characteristics ◽  
Microbiological Analysis ◽  
Mean Square ◽  
Dental Resin ◽  
Irregular Surfaces ◽  
Dental Resins ◽  
Matrix Production

The wide application of additive manufacturing in dentistry implies the further investigation into oral micro-organism adhesion and biofilm formation on vat-photopolymerization (VP) dental resins. The surface characteristics and microbiological analysis of a VP dental resin, printed at resolutions of 50 μm (EG-50) and 100 μm (EG-100), were evaluated against an auto-polymerizing acrylic resin (CG). Samples were evaluated using a scanning electron microscope, a scanning white-light interferometer, and analyzed for Candida albicans (CA) and Streptococcus mutans (SM) biofilm, as well as antifungal and antimicrobial activity. EG-50 and EG-100 exhibited more irregular surfaces and statistically higher mean (Ra) and root-mean-square (rms) roughness (EG-50-Ra: 2.96 ± 0.32 µm; rms: 4.05 ± 0.43 µm / EG-100-Ra: 3.76 ± 0.58 µm; rms: 4.79 ± 0.74 µm) compared to the CG (Ra: 0.52 ± 0.36 µm; rms: 0.84 ± 0.54 µm) (p < 0.05). The biomass and extracellular matrix production by CA and SM and the metabolic activity of SM were significantly decreased in EG-50 and EG-100 compared to CG (p < 0.05). CA and SM growth was inhibited by the pure unpolymerized VP resin (48 h). EG-50 and EG-100 recorded a greater irregularity, higher surface roughness, and decreased CA and SM biofilm formation over the CG.

scholarly journals Candida albicans Denture Biofilm and its Clinical Significance

2012 ◽  
Vol 61 (3) ◽  
pp. 161-167 ◽  
Author(s):  
ANNA D. SEREFKO ◽  
EWA J. POLESZAK ◽  
ANNA MALM
Keyword(s):  
Oral Cavity ◽  
Biofilm Formation ◽  
Three Dimensional ◽  
Acrylic Resin ◽  
Matrix Composition ◽  
Candida Spp ◽  
Specific Complex ◽  
Antibody Titres ◽  
Artificial Teeth ◽  
Germ Tubes

Fungi belonging to Candida genus, especially C. albicans play an important role in microflora of oral cavity. Microbial colonisation process taking place within oral cavity is inseparably related to formation of multispecies biofilm, i.e. dental and denture plaque. A mature fungal biofilm is a heterogeneous three-dimensional dense conglomeration of mixture of different morphological forms: blastospores, germ tubes, pseudohyphae and hyphae surrounded by the extracellular polymeric matrix. Composition and specific properties of substratum, saliva and yeasts as well as multiple intricate interactions between all of them influence the ability of Candida spp. isolates to adhere and colonise both natural and artificial surfaces, followed by biofilm formation. Obviously, specific complex host-pathogen interactions also should not be neglected. A lot of additional factors like poor oral and denture hygiene, low pH under prosthesis, sufficient concentration of sugar and iron or antibody titres influence Candida adhesion and colonisation of acrylic resin base. C. albicans is capable of inducing a variety of superficial diseases of the oral mucosa. The most common clinical form of oral candidal infection related to biofilm formation affecting a great deal of denture wearers is denture-associated stomatitis, also known as chronic atrophic candidiasis or erythemateous candidasis. Development of C. albicans biofilm on a denture surface constitutes a difficult and hard to resolve problem which may concern every single prosthesis-wearer. Thus, careful oral and denture hygiene is highly recommended for the population of artificial teeth wearers.

scholarly journals Evaluation of the Antifungal Activity of the Licania Rigida Leaf Ethanolic Extract against Biofilms Formed by Candida Sp. Isolates in Acrylic Resin Discs

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 250 ◽  
Author(s):  
Maria Audilene de Freitas ◽  
Adryelle Idalina Silva Alves ◽  
Jacqueline Cosmo Andrade ◽  
Melyna Chaves Leite-Andrade ◽  
Antonia Thassya Lucas dos Santos ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Biofilm Formation ◽  
High Performance ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Acrylic Resin ◽  
Planktonic Cells ◽  
Candida Sp ◽  
Flight Mass Spectrometry ◽  
New Compounds

Candida sp. treatment has become a challenge due to the formation of biofilms which favor resistance to conventional antifungals, making the search for new compounds necessary. The objective of this study was to identify the composition of the Licania rigida Benth. leaf ethanolic extract and to verify its antifungal activity against Candida sp. and its biofilms. The composition identification was performed using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique. The antifungal activity of extract and fluconazole against planktonic cells and biofilms was verified through the minimum inhibitory concentration (MIC) following biofilm induction and quantification in acrylic resin discs by reducing tetrazolic salt, with all isolates forming biofilms within 48 h. Six constituents were identified in the extract, and the compounds identified are derivatives from phenolic compounds such as flavonoids (epi) gallocatechin Dimer, epigallocatechin and gallocatechin, Myricetin-O-hexoside, Myricitrin, and Quercetin-O-rhamnoside. The extract reduced biofilm formation in some of the strains analyzed, namely C. tropicalis URM5732, C. krusei INCQS40042, and C. krusei URM6352. This reduction was also observed in the treatment with fluconazole with some of the analyzed strains. The extract showed significant antifungal and anti-biofilm activities with some of the strains tested.

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