Abstract
Abstract 1113
Granulocyte/macrophage colony stimulating factor (GM-CSF) regulates the development and activity of several myeloid cell types, including dendritic cells (DCs), thereby influencing the initiation and maintenance of adaptive immune responses. Mice lacking GM-CSF or its receptor show decreased antigen specific T cell priming against the encephalitogenic peptide of myelin oligodendrocyte glycoprotein (MOG35–55), and are resistant to experimental autoimmune encephalitis (EAE), a mouse model of multiple sclerosis. Beyond this role in priming adaptive immune responses, GM-CSF acts to sustain ongoing T cell effector responses, both in EAE initiated by MOG35–55 peptide and in other models of autoimmunity.
A recent report has argued that part of the role for GM-CSF in EAE is based on its requirement for the development of a subset of dermal DCs, termed CD11blow/−Langerin+CD103+ DCs (J Exp Med. 207:953–61, 2010). These dermal DCs represent one anatomic subtype of peripheral tissue CD11blow/−CD103+ DCs, and share the properties of efficient antigen cross-presentation and IL-12 production with lymphoid tissue-resident CD8α+ DCs. Peripheral tissue CD11blow/−CD103+ DCs and lymphoid tissue-resident CD8α+ DCs are developmentally related, each being dependent on FMS-like tyrosine kinase 3 ligand (Flt3L), and requiring the transcription factors Batf3, Irf8, and Id2 for development. Mice deficient in any one of these transcription factors selectively lack these DC subsets, with Batf3−/− mice representing the best model for studying the role of these DCs in vivo, as Irf8−/− and Id2−/− mice display more widespread immune defects.
Here, we directly compared the development of peripheral tissue DCs and susceptibility to EAE in GM-CSF receptor deficient (Csf2rb−/−) and Batf3−/− mice. We confirmed that Csf2rb−/− mice displayed poor priming of MOG35-55 peptide-specific T cell responses and resisted induction of EAE. Importantly, however, we found that Batf3-dependent dermal CD11blow/−Langerin+ DCs did, in fact, develop in Csf2rb−/− mice, but that these DCs expressed reduced, but not absent, levels of the surface marker CD103. In contrast, Batf3−/− mice, lacking all peripheral CD11blow/− DCs and lymphoid tissue-resident CD8α+ DCs, showed robust Th cell priming after subcutaneous immunization, and were fully susceptible to EAE. These results exclude the hypothesis that defective T effector cell priming and resistance to EAE exhibited by Csf2rb−/− mice result from the absence of dermal CD11blow/−Langerin+CD103+ DCs, and instead suggest that GM-CSF is acting via different mechanisms to promote autoimmunity.
Disclosures:
No relevant conflicts of interest to declare.
CD8+lineage dendritic cells determine adaptive immune responses to inflammasome activation upon sterile skin injury