Evaluation of pre‐slaughter low‐current/high‐frequency electrical stunning on lipid oxidative stability, antioxidant enzyme activity and gene expression of mitogen‐activated protein kinase/nuclear factor erythroid 2‐related factor 2 (MAPK/Nrf2) signalling pathway in thigh muscle of broilers

2019 ◽  
Vol 55 (3) ◽  
pp. 953-960 ◽  
Author(s):  
Lei Xu ◽  
Jing Wang ◽  
Hong‐yuan Yue ◽  
Morgan B. Farnell ◽  
Hai‐ming Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Protein Kinase ◽  
High Frequency ◽  
Antioxidant Enzyme Activity ◽  
Mitogen Activated Protein Kinase ◽  
Thigh Muscle ◽  
Related Factor ◽  
Nuclear Factor ◽  
Mitogen Activated Protein

scholarly journals Natural Astaxanthin From Haematococcus Pluvialis Enhanced Antioxidant Capacity And Improved Semen Quality In Aging Layer Breeder Roosters Through The Mitogen-Activated Protein Kinase/Nuclear Factor-Erythroid 2-Related Factor 2 (MAPK/Nrf2) Pathway

2021 ◽  
Author(s):  
Shan Gao ◽  
Nuo Heng ◽  
Fang Liu ◽  
Yong Guo ◽  
Yu Chen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Antioxidant Capacity ◽  
Hydroxyl Radicals ◽  
Semen Quality ◽  
Mitogen Activated Protein Kinase ◽  
Related Factor ◽  
Mrna Levels ◽  
Nuclear Factor ◽  
Nrf2 Pathway ◽  
Mitogen Activated Protein

Abstract Background: Natural astaxanthin (ASTA) has strong antioxidant properties and has been widely used as a health product to improve human health. However, the effects of ASTA on the reproductive performance of aging roosters have been poorly studied. We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via anti-oxidation defense system.Methods: In the present study, 96 53-week-old Jinghong No. 1 layer breeder roosters were fed a corn-soybean meal basal diet containing ASTA at 0, 25, 50, or 100 mg/kg for 6 week. Results: Semen quality in the ASTA groups remarkably improved as compared to those in the control group and antioxidant activities, the abilities to scavenge hydroxyl radicals and superoxide anions increased gradually with ASTA addition (P < 0.05). In addition, the mRNA levels of antioxidant enzymes, the mRNA and protein levels of the mitogen-activated protein kinase (MAPK), nuclear factor-erythroid 2-related factor 2 (Nrf2) were markedly increased in the 50-100 mg/kg ASTA group (P < 0.05). Conclusions: Collectively, these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities, and the ability to scavenge hydroxyl radicals, which may be related to up-regulation of the MAPK/Nrf2 pathway.

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