scholarly journals Natural Astaxanthin From Haematococcus Pluvialis Enhanced Antioxidant Capacity And Improved Semen Quality In Aging Layer Breeder Roosters Through The Mitogen-Activated Protein Kinase/Nuclear Factor-Erythroid 2-Related Factor 2 (MAPK/Nrf2) Pathway

2021 ◽  
Author(s):  
Shan Gao ◽  
Nuo Heng ◽  
Fang Liu ◽  
Yong Guo ◽  
Yu Chen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Antioxidant Capacity ◽  
Hydroxyl Radicals ◽  
Semen Quality ◽  
Mitogen Activated Protein Kinase ◽  
Related Factor ◽  
Mrna Levels ◽  
Nuclear Factor ◽  
Nrf2 Pathway ◽  
Mitogen Activated Protein

Abstract Background: Natural astaxanthin (ASTA) has strong antioxidant properties and has been widely used as a health product to improve human health. However, the effects of ASTA on the reproductive performance of aging roosters have been poorly studied. We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via anti-oxidation defense system.Methods: In the present study, 96 53-week-old Jinghong No. 1 layer breeder roosters were fed a corn-soybean meal basal diet containing ASTA at 0, 25, 50, or 100 mg/kg for 6 week. Results: Semen quality in the ASTA groups remarkably improved as compared to those in the control group and antioxidant activities, the abilities to scavenge hydroxyl radicals and superoxide anions increased gradually with ASTA addition (P < 0.05). In addition, the mRNA levels of antioxidant enzymes, the mRNA and protein levels of the mitogen-activated protein kinase (MAPK), nuclear factor-erythroid 2-related factor 2 (Nrf2) were markedly increased in the 50-100 mg/kg ASTA group (P < 0.05). Conclusions: Collectively, these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities, and the ability to scavenge hydroxyl radicals, which may be related to up-regulation of the MAPK/Nrf2 pathway.

scholarly journals Natural astaxanthin enhanced antioxidant capacity and improved semen quality through the MAPK/Nrf2 pathway in aging layer breeder roosters

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shan Gao ◽  
Nuo Heng ◽  
Fang Liu ◽  
Yong Guo ◽  
Yu Chen ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Hydroxyl Radicals ◽  
Semen Quality ◽  
Antioxidant Properties ◽  
Mitogen Activated Protein Kinase ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Related Factor ◽  
Mrna Levels ◽  
Nrf2 Pathway

Abstract Background Natural astaxanthin (ASTA) has strong antioxidant properties and has been widely used as a health product to improve human health. However, the effects of ASTA on the reproductive performance of aging roosters have been poorly studied. We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via anti-oxidation defense system. Methods In the present study, 96 53-week-old Jinghong No. 1 layer breeder roosters were fed a corn-soybean meal basal diet containing 0, 25, 50, or 100 mg/kg ASTA for 6 weeks. Results Semen quality in the ASTA groups remarkably improved than that in the control group, and antioxidant activities, the abilities to scavenge hydroxyl radicals and superoxide anions, increased gradually with ASTA addition (P < 0.05). In addition, the mRNA levels of antioxidant enzymes as well as the mRNA and protein levels of the mitogen-activated protein kinase (MAPK) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were markedly increased in the 50–100 mg/kg ASTA group (P < 0.05). Conclusions Collectively, these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities and the ability to scavenge hydroxyl radicals, which may be related to upregulation of the MAPK/Nrf2 pathway.

scholarly journals Dietary Lysine Levels Improved Antioxidant Capacity and Immunity via the TOR and p38 MAPK Signaling Pathways in Grass Carp, Ctenopharyngodon idellus Fry

2021 ◽  
Vol 12 ◽  
Author(s):  
Dongyu Huang ◽  
Sahya Maulu ◽  
Mingchun Ren ◽  
Hualiang Liang ◽  
Xianping Ge ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Grass Carp ◽  
Control Diet ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Target Of Rapamycin ◽  
Interleukin 4 ◽  
Mrna Levels ◽  
Nuclear Factor ◽  
Mitogen Activated Protein

An 8-week rearing trial was designed to appraise the dietary lysine levels on intestinal antioxidant capacity and immunity of grass carp fry. Six practical diets were prepared with graded levels of lysine (1.44, 1.79, 1.97, 2.44, 2.56 and 2.87% dry matter), and these diets were fed to grass carp fry. The results showed that the activities of intestinal antioxidant factors including catalase and glutathione peroxidase were markedly improved by the 2.44% dietary lysine compared with the control diet (1.44% dietary lysine) (P &lt; 0.05). In terms of antioxidants, compared with the control diet, the 2.44% diet markedly upregulated the mRNA expression levels of target of rapamycin, S6 kinase1 and nuclear factor erythroid 2-related factor 2 pathway-related antioxidant genes, containing catalase and glutathione peroxidase 1α (P &lt; 0.05) and downregulated the mRNA levels of Kelch-like ECH-associated protein 1 (P &gt; 0.05). The mRNA levels of 4E-binding protein 2 showed the opposite trend compared with those of target of rapamycin, and the minimum value was observed in the group of 1.97% dietary lysine (P &lt; 0.05). In terms of immunity, compared with the 1.44% diet, the 2.44% diet markedly suppressed the intestinal p38 mitogen-activated protein kinase and interferon γ2 mRNA levels (P &lt; 0.05). Moreover, nuclear factor-kappa B p65, tumor necrosis factor α, interleukin 6, interleukin 8, and interleukin 15 mRNA levels all exhibited the same trend as p38 mitogen-activated protein kinase and interferon γ2; however, the difference among all the lysine treatments groups was not significant (P &gt; 0.05). The anti-inflammatory cytokines transforming growth factor β2 and interleukin 4/13B mRNA levels in the intestine were remarkably upregulated by high dietary lysine levels (2.56 and 2.87%) (P &lt; 0.05), and when the dietary lysine level reached 2.44%, the interleukin 4/13A mRNA levels were strikingly increased (P &lt; 0.05). Overall, the data suggested that 2.44% dietary lysine could strengthen the immune and antioxidant capacities of grass carp fry via activating the target of rapamycin (TOR) signaling pathway, and suppressing the p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway, which then improve the survival rate.

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