Gene expression in Lucilia sericata (Diptera: Calliphoridae) larvae exposed to Pseudomonas aeruginosa and Acinetobacter baumannii identifies shared and microbe‐specific induction of immune genes

Antibiotic resistance is a continuing challenge in medicine. There are various strategies for expanding antibiotic therapeutic repertoires, including the use of blow flies. Their larvae exhibit strong antibiotic and antibiofilm properties that alter microbiome communities. One species, Lucilia sericata, is used to treat problematic wounds due to its debridement capabilities and its excretions and secretions that kill some pathogenic bacteria. There is much to be learned about how L. sericata interacts with microbiomes at the molecular level. To address this deficiency, gene expression was assessed after feeding exposure (1 hour or 4 hours) to two clinically problematic pathogens: Pseudomonas aeruginosa and Acinetobacter baumanii. The results identified immunity related genes that were differentially expressed when exposed to these pathogens, as well as non-immune genes possibly involved in gut responses to bacterial infection. There was a greater response to P. aeruginosa that increased over time, while few genes responded to A. baumanii exposure and expression was not time-dependent. The response to feeding on pathogens indicates a few common responses and features distinct to each pathogen, which is useful in improving wound debridement therapy and helps develop biomimetic alternatives.

Download Full-text

Decreased carO gene expression and OXA-type carbapenemases among extensively drug-resistant Acinetobacter baumannii strains isolated from burn patients in Tehran, Iran

Acta Microbiologica et Immunologica Hungarica ◽

10.1556/030.2020.01138 ◽

2020 ◽

Cited By ~ 1

Author(s):

Elham Abbasi ◽

Hossein Goudarzi ◽

Ali Hashemi ◽

Alireza Salimi Chirani ◽

Abdollah Ardebili ◽

...

Keyword(s):

Gene Expression ◽

Acinetobacter Baumannii ◽

High Rate ◽

Burn Patients ◽

Drug Resistant ◽

Disc Diffusion ◽

Carbapenem Resistant ◽

Extensively Drug Resistant ◽

Sds Page ◽

Extensively Drug Resistance

AbstractA major challenge in the treatment of infections has been the rise of extensively drug resistance (XDR) and multidrug resistance (MDR) in Acinetobacter baumannii. The goals of this study were to determine the pattern of antimicrobial susceptibility, blaOXA and carO genes among burn-isolated A. baumannii strains. In this study, 100 A. baumannii strains were isolated from burn patients and their susceptibilities to different antibiotics were determined using disc diffusion testing and broth microdilution. Presence of carO gene and OXA-type carbapenemase genes was tested by PCR and sequencing. SDS-PAGE was done to survey CarO porin and the expression level of carO gene was evaluated by Real-Time PCR. A high rate of resistance to meropenem (98%), imipenem (98%) and doripenem (98%) was detected. All tested A. baumannii strains were susceptible to colistin. The results indicated that 84.9% were XDR and 97.9% of strains were MDR. In addition, all strains bore blaOXA-51 like and blaOXA-23 like and carO genes. Nonetheless, blaOXA-58 like and blaOXA-24 like genes were harbored by 0 percent and 76 percent of strains, respectively. The relative expression levels of the carO gene ranged from 0.06 to 35.01 fold lower than that of carbapenem-susceptible A. baumannii ATCC19606 and SDS – PAGE analysis of the outer membrane protein showed that all 100 isolates produced CarO. The results of current study revealed prevalence of blaOXA genes and changes in carO gene expression in carbapenem resistant A.baumannii.

Download Full-text

Faculty Opinions recommendation of Modulation of Pseudomonas aeruginosa gene expression by host microflora through interspecies communication.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1002877.201723 ◽

2004 ◽

Author(s):

Beth Lazazzera

Keyword(s):

Gene Expression ◽

Pseudomonas Aeruginosa ◽

Interspecies Communication

Download Full-text

Exploiting Quorum Sensing Inhibition for the Control of Pseudomonas aeruginosa and Acinetobacter baumannii Biofilms

Current Topics in Medicinal Chemistry ◽

10.2174/1568026617666170105144104 ◽

2017 ◽

Vol 17 (17) ◽

pp. 1915-1927 ◽

Cited By ~ 14

Author(s):

Israel Castillo-Juarez ◽

Luis Esau Lopez-Jacome ◽

Gloria Soberon-Chavez ◽

Maria Tomas ◽

Jintae Lee ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Acinetobacter Baumannii ◽

Quorum Sensing Inhibition

Download Full-text

New Insights into the Effect of Origanum Extracts on the Gene Expression Profiles of Multidrug-resistant Isolates of Pseudomonas aeruginosa Retrieved from Oreochromis niloticus

Turkish Journal of Fisheries and Aquatic Sciences ◽

10.4194/1303-2712-v20_7_01 ◽

2020 ◽

Vol 20 (7) ◽

Cited By ~ 2

Author(s):

Ali Wahdan ◽

Amr Fadel ◽

Mahmoud Mabrok

Keyword(s):

Gene Expression ◽

Pseudomonas Aeruginosa ◽

Oreochromis Niloticus ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Multidrug Resistant

Download Full-text

Microbiological and molecular genetic aspects of the antibiotic resistance of Pseudomonas aeruginosa and Acinetobacter baumannii

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2020.09.182 ◽

2020 ◽

Vol 101 ◽

pp. 57-58

Author(s):

Y. Skurikhina ◽

V. Turkutjukov

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Acinetobacter Baumannii ◽

Molecular Genetic

Download Full-text

Human Pleural Fluid and Human Serum Albumin Modulate the Behavior of a Hypervirulent and Multidrug-Resistant (MDR) Acinetobacter baumannii Representative Strain

Pathogens ◽

10.3390/pathogens10040471 ◽

2021 ◽

Vol 10 (4) ◽

pp. 471

Author(s):

Camila Pimentel ◽

Casin Le ◽

Marisel R. Tuttobene ◽

Tomas Subils ◽

Jasmine Martinez ◽

...

Keyword(s):

Gene Expression ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Acinetobacter Baumannii ◽

Pleural Fluid ◽

Gene Expression Profile ◽

Expression Profile ◽

The United States ◽

World Health

Acinetobacter baumannii is a nosocomial pathogen capable of causing serious infections associated with high rates of morbidity and mortality. Due to its antimicrobial drug resistance profile, A. baumannii is categorized as an urgent priority pathogen by the Centers for Disease Control and Prevention in the United States and a priority group 1 critical microorganism by the World Health Organization. Understanding how A. baumannii adapts to different host environments may provide critical insights into strategically targeting this pathogen with novel antimicrobial and biological therapeutics. Exposure to human fluids was previously shown to alter the gene expression profile of a highly drug-susceptible A. baumannii strain A118 leading to persistence and survival of this pathogen. Herein, we explore the impact of human pleural fluid (HPF) and human serum albumin (HSA) on the gene expression profile of a highly multi-drug-resistant strain of A. baumannii AB5075. Differential expression was observed for ~30 genes, whose products are involved in quorum sensing, quorum quenching, iron acquisition, fatty acid metabolism, biofilm formation, secretion systems, and type IV pilus formation. Phenotypic and further transcriptomic analysis using quantitative RT-PCR confirmed RNA-seq data and demonstrated a distinctive role of HSA as the molecule involved in A. baumannii’s response.

Download Full-text