The relationship between the S system of blood groups and potassium levels in red blood cells of cattle

2009 ◽  
Vol 5 (2) ◽  
pp. 95-104 ◽  
Author(s):  
B. A. Rasmusen ◽  
Elizabeth M. Tucker ◽  
J. C. Ellory ◽  
R. L. Spooner
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Groups ◽  
The Relationship

scholarly journals The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

2019 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Dora Dayu Rahma Turista ◽  
Eka Puspitasari
Keyword(s):  
Health Education ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Pathogenic Bacteria ◽  
Agar Plate ◽  
Blood Groups ◽  
Blood Agar Plate ◽  
Sheep Blood ◽  
Randomized Design

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.

Volume-dependent K+ transport in rabbit red blood cells comparison with oxygenated human SS cells

1989 ◽  
Vol 257 (1) ◽  
pp. C114-C121 ◽  
Author(s):  
N. al-Rohil ◽  
M. L. Jennings
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cell Volume Regulation ◽  
Sulfhydryl Group ◽  
Cell Swelling ◽  
Inhibitory Potency ◽  
Ph Optimum ◽  
The Relationship ◽  
Low K ◽  
K Transport

In this study the volume-dependent or N-ethylmaleimide (NEM)-stimulated, ouabain-insensitive K+ influx and efflux were measured with the tracer 86Rb+ in rabbit red blood cells. The purpose of the work was to examine the rabbit as a potential model for cell volume regulation in human SS red blood cells and also to investigate the relationship between the NEM-reactive sulfhydryl group(s) and the signal by which cell swelling activates the transport. Ouabain-resistant K+ efflux and influx increase nearly threefold in cells swollen hypotonically by 15%. Pretreatment with 2 mM NEM stimulates efflux 5-fold and influx 10-fold (each measured in an isotonic medium). The ouabain-resistant K+ efflux was dependent on the major anion in the medium. The anion dependence of K+ efflux in swollen or NEM-stimulated cells was as follows: Br- greater than Cl- much greater than NO3- = acetate. The magnitudes of both the swelling- and the NEM-stimulated fluxes are much higher in young cells (density separated but excluding reticulocytes) than in older cells. Swelling- or NEM-stimulated K+ efflux in rabbit red blood cells was inhibited 50% by 1 mM furosemide, and the inhibitory potency of furosemide was enhanced by extracellular K+, as is known to be true for human AA and low-K+ sheep red blood cells. The swelling-stimulated flux in both rabbit and human SS cells has a pH optimum at approximately 7.4. We conclude that rabbit red blood cells are a good model for swelling-stimulated K+ transport in human SS cells.(ABSTRACT TRUNCATED AT 250 WORDS)

The rates of ABO, Rh, and Kell antigens in children with cancer.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e22007-e22007
Author(s):  
Andrey A. Maslov ◽  
Nailya Guskova ◽  
Ekaterina Guskova ◽  
Kristina Avanesova ◽  
Svetlana V. Belgova ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Subsequent Development ◽  
European Population ◽  
Blood Groups ◽  
Abo Blood Groups ◽  
Phenotypic Characteristics ◽  
Children With Cancer ◽  
K Antigen

e22007 Background: The purpose of the study was to analyze phenotypic characteristics of red blood cells by the AVBO, Rh and Kell systems in children with cancer. Methods: ABO and Rh blood groups were determined and erythrocyte antigens (D, С, с, Сw, Е, е, К, k) were typed (AutoVue Innova, USA) in blood samples of 114 children with solid tumors. Results: ABO blood groups distribution was as follows: A(II) > O(I) > B(III) > AB(IV) with A(II) prevalence. Rh(D)-positive phenotype was observed in 82 (71.9%) patients of 114: 47 (57.3%) boys and 35 (42.6%) girls. 32 (28.1%) patients of 114 were Rh(D)-negative: 15 (46.8%) boys and 17 (53.1%) girls. Only 8 (7%) children were Kell-positive, which was similar to the antigen prevalence in European population. 4 erythrocyte phenotypes were the most frequent in Rh(D)-positive patients: СсDееK− (34.1%), ССDееK− (22.0%), ccDEeK− (13.4%) and СсDЕеK− (11.0%). I.e., more than a half of children with oncopathologies (56.1%) had Kell-negative phenotypes, СсDееK− and ССDееK−. 86.4% of Rh(D)-positive patients had homozygous combinations of Rhesus antigens causing transfusion reactions - СС, сc, ЕЕ and ее. 18 (22.0%) of Rh(D)-positive patients were homozygous for the C antigen and 64 (78.0%), i.e. every third patient, had the c antigen. Children with the C antigen may be sensitized to the c antigen through blood transfusion with subsequent development of hemolytic complications. The K (Cellano) antigen was found in all children, and 93% of them had kk phenotype and 7% - Kk. The Сw (Willis) antigen was revealed only in 5 (6.0%) Rh(D)-positive patients with rare phenotypes - CwCceeK-, CwccEeK-, CwCcEEK-, CwCcEeK-. Matching a donor for patients with one of these phenotypes could pose a problem. Conclusions: Studying phenotypic characteristics of red blood cells is necessary for providing a successful blood transfusion, especially in children Kell-positive for the K antigen, in children homozygous for the C antigen with ССDееК- phenotype and in children with the Сw antigen and СwСсееК-, СwссЕеК-, СwСсЕЕК- and СwСсЕеК- phenotypes.

scholarly journals Distribution of Blood Groups in Medical Students: A Comparative Study

2020 ◽  
Vol 8 (1) ◽  
pp. 42-46
Author(s):  
Bishal Joshi ◽  
Sanjit Kumar Kar ◽  
Shankar Yadav ◽  
Prem Kumar Yadav ◽  
Narayan Bahadur Mahotra ◽  
...  
Keyword(s):  
Medical Students ◽  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Blood Groups ◽  
Test Method ◽  
Fetal Life ◽  
Human Red Blood Cells ◽  
Slide Test ◽  
Group B

INTRODUCTION: Blood groups depend on antigens present on the surface of red blood cells. Scientists have discovered at least 30 common antigens and hundreds of rare antigens causing antigen-antibody reaction in human red blood cells. These antigens are genetically determined and are developed in fetal life and remain unchanged till death. Many blood group systems are identified but ABO and Rh blood groups are more antigenic. MATERIAL AND METHODS: In the present study, we observed ABO and Rh blood groups of 3057 students who studied in Universal College of Medical Sciences, Bhairahawa, Nepal from 1998 AD to 2019 AD, using open slide test method. The data was analyzed using SPSS version 20. RESULTS: Results  of the present study indicated that the most common blood group was O (36.8%)  followed by blood group B (31.1%) and blood group A (24.9%) and least common blood group was AB (7.2%) i.e. O>B>A>AB. The same sequence of ABO blood grouping was seen in both male and female.  Rh positive blood group was found in 95.4% and Rh negative blood group was found in 4.6% of population. CONCLUSION: Knowledge of distribution of blood group is very important for medical students as they can serve as immediate blood donor in emergency conditions. This study can provide insight to advanced studies in future which can relate blood groups with medical conditions.

The Relationship Between KCl Infusion and Changes of ECG, Electrolytes of Plasma and K Content of Donkey's Red Blood Cells

2008 ◽  
Vol 11 (3) ◽  
pp. 433-437 ◽  
Author(s):  
M. Pourjafar ◽  
G.H.A. Kojouri ◽  
A. Jafari Dehkordi ◽  
S. Sharifi ◽  
T. Esmaeili
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
The Relationship
Sign in / Sign up

Export Citation Format

Share Document