The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.

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Exploring the possibilities of infrared spectroscopy for urine sediment examination and detection of pathogenic bacteria in urinary tract infections

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2020-0524 ◽

2020 ◽

Vol 58 (10) ◽

pp. 1759-1767

Author(s):

Mieke Steenbeke ◽

Sander De Bruyne ◽

Jerina Boelens ◽

Matthijs Oyaert ◽

Griet Glorieux ◽

...

Keyword(s):

Infrared Spectroscopy ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Pathogenic Bacteria ◽

Agar Plate ◽

Principal Component ◽

Blood Agar Plate ◽

Urine Sediment ◽

Flight Mass Spectrometry ◽

Tract Infections

AbstractObjectivesIn this study, the possibilities of Fourier-transformed infrared spectroscopy (FTIR) for analysis of urine sediments and for detection of bacteria causing urinary tract infections (UTIs) were investigated.MethodsDried urine specimens of control subjects and patients presenting with various nephrological and urological conditions were analysed using mid-infrared spectroscopy (4,000–400 cm−1). Urine samples from patients with a UTI were inoculated on a blood agar plate. After drying of the pure bacterial colonies, FTIR was applied and compared with the results obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chemometric data analysis was used to classify the different species.ResultsDue to the typical molecular assignments of lipids, proteins, nucleic acids and carbohydrates, FTIR was able to identify bacteria and showed promising results in the detection of proteins, lipids, white and red blood cells, as well as in the identification of crystals. Principal component analysis (PCA) allowed to differentiate between Gram-negative and Gram-positive species and soft independent modelling of class analogy (SIMCA) revealed promising classification ratios between the different pathogens.ConclusionsFTIR can be considered as a supplementary method for urine sediment examination and for detection of pathogenic bacteria in UTI.

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Portable vibration-assisted filtration device for on-site isolation of blood cells or pathogenic bacteria from whole human blood

Talanta ◽

10.1016/j.talanta.2017.09.075 ◽

2018 ◽

Vol 179 ◽

pp. 207-212 ◽

Cited By ~ 3

Author(s):

Yong Tae Kim ◽

Kyun Joo Park ◽

Seyl Kim ◽

Soon Ae Kim ◽

Seok Jae Lee ◽

...

Keyword(s):

Human Blood ◽

Blood Cells ◽

Pathogenic Bacteria ◽

Site Isolation ◽

Filtration Device

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The Effect of Breadfruit Leaf Flour (Artocarpus altilis) on Number of Blood Cells And Correlation Between Cholesterol Blood and Meat of Tegal Ducks 10 Weeks Age

ANIMAL PRODUCTION ◽

10.20884/1.jap.2017.19.3.635 ◽

2018 ◽

Vol 19 (3) ◽

pp. 179

Author(s):

Elly Tugiyanti ◽

Emmy Susanti

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Regression Coefficient ◽

White Blood Cells ◽

Blood Cholesterol ◽

Blood Profile ◽

Artocarpus Altilis ◽

Randomized Design ◽

Leaf Powder ◽

Completely Randomized Design

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old. The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022).

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Merocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum

Blood ◽

10.1182/blood.v80.1.21.bloodjournal80121 ◽

1992 ◽

Vol 80 (1) ◽

pp. 21-24 ◽

Cited By ~ 1

Author(s):

OM Smith ◽

SA Dolan ◽

JA Dvorak ◽

TE Wellems ◽

F Sieber

Keyword(s):

Stem Cells ◽

Plasmodium Falciparum ◽

Hematopoietic Stem Cells ◽

Red Blood Cells ◽

Human Blood ◽

Blood Cells ◽

Potential Usefulness ◽

Hematopoietic Stem ◽

Infected Blood ◽

Merocyanine 540

The purpose of this study was to evaluate the photosensitizing dye merocyanine 540 (MC540) as a means for extracorporeal purging of Plasmodium falciparum-infected erythrocytes from human blood. Parasitized red blood cells bound more dye than nonparasitized cells, and exposure to MC540 and light under conditions that are relatively well tolerated by normal erythrocytes and normal pluripotent hematopoietic stem cells reduced the concentration of parasitized cells by as much as 1,000-fold. Cells parasitized by the chloroquine- sensitive HB3 clone and the chloroquine-resistant Dd2 clone of P falciparum were equally susceptible to MC540-sensitized photolysis. These data suggest the potential usefulness of MC540 in the purging of P falciparum-infected blood.

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Intrinsically magnetic susceptibility in human blood and its impact on cell separation: Non-classical and intermediate monocytes have the strongest magnetic behavior in fresh human blood

10.22541/au.161108032.20496584/v1 ◽

2021 ◽

Author(s):

Jenifer Gómez-Pastora ◽

James Kim ◽

Victor Multanen ◽

Mitchell Weigand ◽

Nicole Walters ◽

...

Keyword(s):

Magnetic Properties ◽

Red Blood Cells ◽

Human Blood ◽

Blood Cells ◽

Cell Tracking ◽

Magnetic Behavior ◽

Immunomagnetic Separation ◽

Iron Storage ◽

Cell Tracking Velocimetry ◽

Classical Monocytes

The presence of iron in circulating monocytes is well known as they play an essential role in iron recycling. It has been demonstrated that the iron content of blood cells can be measured through their magnetic behavior; however, the magnetic properties of different monocyte subtypes remain unknown. In this study, we report for the first time, the magnetic behavior of classical, intermediate and non-classical monocytes, which is related to their iron storage capacity. The magnetic properties of monocytes were compared to other blood cells, such as lymphocytes and red blood cells in the oxyhemoglobin and methemoglobin states, and a cancer cell type. For this analysis, we used an instrument referred to as Cell Tracking Velocimetry (CTV), which quantitatively characterizes the magnetic behavior of biological entities. Our results demonstrate that significant fractions of the intermediate and non-classical monocytes have high magnetophoretic mobilities, equivalent to methemoglobin red blood cells and higher than the classical subset, suggesting their higher iron storage capacities. Moreover, our findings have implications for the immunomagnetic separation industry; we demonstrate that negative magnetic isolation techniques for recovering monocytes from blood should be used with caution, as it is possible to lose magnetic monocytes when using this technique.

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Plasma skimming efficiency of human blood in the spiral groove bearing of a centrifugal blood pump

Journal of Artificial Organs ◽

10.1007/s10047-020-01221-9 ◽

2020 ◽

Author(s):

Daisuke Sakota ◽

Kazuki Kondo ◽

Ryo Kosaka ◽

Masahiro Nishida ◽

Osamu Maruyama

Keyword(s):

Red Blood Cells ◽

Human Blood ◽

Blood Cells ◽

High Speed ◽

Blood Pump ◽

Spiral Groove ◽

Centrifugal Blood Pump ◽

Plasma Skimming ◽

Ridge Area ◽

Spiral Groove Bearing

Abstract This work investigates the plasma skimming effect in a spiral groove bearing within a hydrodynamically levitated centrifugal blood pump when working with human blood having a hematocrit value from 0 to 40%. The present study assessed the evaluation based on a method that clarified the limitations associated with such assessments. Human blood was circulated in a closed-loop circuit via a pump operating at 4000 rpm at a flow rate of 5 L/min. Red blood cells flowing through a ridge area of the bearing were directly observed using a high-speed microscope. The hematocrit value in the ridge area was calculated using the mean corpuscular volume, the bearing gap, the cross-sectional area of a red blood cell, and the occupancy of red blood cells. The latter value was obtained from photographic images by dividing the number of pixels showing red blood cells in the evaluation area by the total number of pixels in this area. The plasma skimming efficiency was calculated as the extent to which the hematocrit of the working blood was reduced in the ridge area. For the hematocrit in the circuit from 0 to 40%, the plasma skimming efficiency was approximately 90%, meaning that the hematocrit in the ridge area became 10% as compared to that in the circuit. For a hematocrit of 20% and over, red blood cells almost completely occupied the ridge. Thus, a valid assessment of plasma skimming was only possible when the hematocrit was less than 20%.

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Assessment of prion reduction filters in decreasing infectivity of ultracentrifuged 263K scrapie-infected brain homogenates in “spiked” human blood and red blood cells

Transfusion ◽

10.1111/trf.12369 ◽

2013 ◽

Vol 54 (4) ◽

pp. 990-995 ◽

Cited By ~ 5

Author(s):

Franco Cardone ◽

Samuel Sowemimo-Coker ◽

Hanin Abdel-Haq ◽

Marco Sbriccoli ◽

Silvia Graziano ◽

...

Keyword(s):

Red Blood Cells ◽

Human Blood ◽

Blood Cells

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Polyphenols enhance total oxidant-scavenging capacities of human blood by binding to red blood cells

Experimental Biology and Medicine ◽

10.1258/ebm.2010.009370 ◽

2010 ◽

Vol 235 (6) ◽

pp. 689-699 ◽

Cited By ~ 74

Author(s):

Erez Koren ◽

Ron Kohen ◽

Isaac Ginsburg

Keyword(s):

Red Blood Cells ◽

Human Blood ◽

Blood Cells

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The rates of ABO, Rh, and Kell antigens in children with cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e22007 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e22007-e22007

Author(s):

Andrey A. Maslov ◽

Nailya Guskova ◽

Ekaterina Guskova ◽

Kristina Avanesova ◽

Svetlana V. Belgova ◽

...

Keyword(s):

Blood Transfusion ◽

Red Blood Cells ◽

Blood Cells ◽

Subsequent Development ◽

European Population ◽

Blood Groups ◽

Abo Blood Groups ◽

Phenotypic Characteristics ◽

Children With Cancer ◽

K Antigen

e22007 Background: The purpose of the study was to analyze phenotypic characteristics of red blood cells by the AVBO, Rh and Kell systems in children with cancer. Methods: ABO and Rh blood groups were determined and erythrocyte antigens (D, С, с, Сw, Е, е, К, k) were typed (AutoVue Innova, USA) in blood samples of 114 children with solid tumors. Results: ABO blood groups distribution was as follows: A(II) > O(I) > B(III) > AB(IV) with A(II) prevalence. Rh(D)-positive phenotype was observed in 82 (71.9%) patients of 114: 47 (57.3%) boys and 35 (42.6%) girls. 32 (28.1%) patients of 114 were Rh(D)-negative: 15 (46.8%) boys and 17 (53.1%) girls. Only 8 (7%) children were Kell-positive, which was similar to the antigen prevalence in European population. 4 erythrocyte phenotypes were the most frequent in Rh(D)-positive patients: СсDееK− (34.1%), ССDееK− (22.0%), ccDEeK− (13.4%) and СсDЕеK− (11.0%). I.e., more than a half of children with oncopathologies (56.1%) had Kell-negative phenotypes, СсDееK− and ССDееK−. 86.4% of Rh(D)-positive patients had homozygous combinations of Rhesus antigens causing transfusion reactions - СС, сc, ЕЕ and ее. 18 (22.0%) of Rh(D)-positive patients were homozygous for the C antigen and 64 (78.0%), i.e. every third patient, had the c antigen. Children with the C antigen may be sensitized to the c antigen through blood transfusion with subsequent development of hemolytic complications. The K (Cellano) antigen was found in all children, and 93% of them had kk phenotype and 7% - Kk. The Сw (Willis) antigen was revealed only in 5 (6.0%) Rh(D)-positive patients with rare phenotypes - CwCceeK-, CwccEeK-, CwCcEEK-, CwCcEeK-. Matching a donor for patients with one of these phenotypes could pose a problem. Conclusions: Studying phenotypic characteristics of red blood cells is necessary for providing a successful blood transfusion, especially in children Kell-positive for the K antigen, in children homozygous for the C antigen with ССDееК- phenotype and in children with the Сw antigen and СwСсееК-, СwссЕеК-, СwСсЕЕК- and СwСсЕеК- phenotypes.

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