The role of chromatin-remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination

2007 ◽  
Vol 52 (5) ◽  
pp. 927-936 ◽  
Author(s):  
Elian Perruc ◽  
Natsuko Kinoshita ◽  
Luis Lopez-Molina
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Chromatin Remodeling ◽  
Stress Responses ◽  
Arabidopsis Seed

scholarly journals A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shuangtao Li ◽  
Zhirong Wang ◽  
Fei Wang ◽  
Hongmei Lv ◽  
Meng Cao ◽  
...  
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Stomatal Closure ◽  
Negative Regulator ◽  
Biotic And Abiotic Stress ◽  
Growth Activity ◽  
A Subunit ◽  
Antioxidant Enzymes Activities

Abstract Background TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. Results In cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure. Conclusion Under osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.

An LRR-only protein regulates abscisic acid-mediated abiotic stress responses during Arabidopsis seed germination

2020 ◽  
Vol 39 (7) ◽  
pp. 909-920
Author(s):  
Pratibha Ravindran ◽  
Shi Yin Yong ◽  
Bijayalakshmi Mohanty ◽  
Prakash P. Kumar
Keyword(s):  
Abscisic Acid ◽  
Abiotic Stress ◽  
Seed Germination ◽  
Stress Responses ◽  
Arabidopsis Seed

scholarly journals Role of Silicon in Mediating Salt Tolerance in Plants: A Review

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 147 ◽  
Author(s):  
Yong-Xing Zhu ◽  
Hai-Jun Gong ◽  
Jun-Liang Yin
Keyword(s):  
Oxidative Stress ◽  
Salt Stress ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Polyamine Metabolism ◽  
Research Areas ◽  
Aquaporin Gene ◽  
Ion Imbalance ◽  
The Impact

Salt stress is a major threat for plant growth worldwide. The regulatory mechanisms of silicon in alleviating salt stress have been widely studied using physiological, molecular genetics, and genomic approaches. Recently, progresses have been made in elucidating the alleviative effects of silicon in salt-induced osmotic stress, Na toxicity, and oxidative stress. In this review, we highlight recent development on the impact of silicon application on salt stress responses. Emphasis will be given to the following aspects. (1) Silicon transporters have been experimentally identified in different plant species and their structure feature could be an important molecular basis for silicon permeability. (2) Silicon could mediate salt-induced ion imbalance by (i) regulating Na+ uptake, transport, and distribution and (ii) regulating polyamine levels. (3) Si-mediated upregulation of aquaporin gene expression and osmotic adjustment play important roles in alleviating salinity-induced osmotic stress. (4) Silicon application direct/indirectly mitigates oxidative stress via regulating the antioxidant defense and polyamine metabolism. (5) Omics studies reveal that silicon could regulate plants’ response to salt stress by modulating the expression of various genes including transcription factors and hormone-related genes. Finally, research areas that require further investigation to provide a deeper understanding of the role of silicon in plants are highlighted.

scholarly journals Role of a Heterotrimeric G Protein in Regulation of Arabidopsis Seed Germination

2002 ◽  
Vol 129 (2) ◽  
pp. 897-907 ◽  
Author(s):  
Hemayet Ullah ◽  
Jin-Gui Chen ◽  
Shucai Wang ◽  
Alan M. Jones
Keyword(s):  
Seed Germination ◽  
G Protein ◽  
Heterotrimeric G Protein ◽  
Arabidopsis Seed

scholarly journals The Arabidopsis SENESCENCE-ASSOCIATED GENE 13 Regulates Dark-Induced Senescence and Plays Contrasting Roles in Defense Against Bacterial and Fungal Pathogens

2020 ◽  
Vol 33 (5) ◽  
pp. 754-766 ◽  
Author(s):  
Nikhilesh Dhar ◽  
Julie Caruana ◽  
Irmak Erdem ◽  
Krishna V. Subbarao ◽  
Steven J. Klosterman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Seed Germination ◽  
Stress Responses ◽  
Fungal Pathogens ◽  
Crop Improvement ◽  
Anthocyanin Accumulation ◽  
Normal Seed ◽  
Conserved Gene

SENESCENCE-ASSOCIATED GENE 13 (SAG13) of Arabidopsis is a widely conserved gene of unknown function that has been extensively used as a marker of plant senescence. SAG13 induction occurs during plant cell death processes, including senescence and hypersensitive response, a type of programmed cell death that occurs in response to pathogens. This implies that SAG13 expression is regulated through at least two different signaling pathways affecting these two different processes. Our work highlights a contrasting role for SAG13 in regulating resistance against disease-causing biotrophic bacterial and necrotrophic fungal pathogens with contrasting infection strategies. We provide further evidence that SAG13 is not only induced during oxidative stress but also plays a role in protecting the plant against other stresses. SAG13 is also required for normal seed germination, seedling growth, and anthocyanin accumulation. The work presented here provides evidence for the role of SAG13 in regulating multiple plant processes including senescence, defense, seed germination, and abiotic stress responses. SAG13 is a valuable molecular marker for these processes and is conserved in multiple plant species, and this knowledge has important implications for crop improvement.

scholarly journals Role of proP and proU in Betaine Uptake by Yersinia enterocolitica under Cold and Osmotic Stress Conditions

2008 ◽  
Vol 75 (6) ◽  
pp. 1471-1477 ◽  
Author(s):  
Thirunavukkarasu Annamalai ◽  
Kumar Venkitanarayanan
Keyword(s):  
Osmotic Stress ◽  
Yersinia Enterocolitica ◽  
Stress Responses ◽  
Wild Type ◽  
Intracellular Accumulation ◽  
Cold Temperatures ◽  
High Osmolarity ◽  
Food Borne ◽  
In The Wild

ABSTRACT Yersinia enterocolitica is a food-borne pathogen with the ability to grow at cold temperatures and tolerate high osmolarity. The bacterium tolerates osmotic stress by intracellular accumulation of osmolytes, such as betaine. The proP gene and proU operon of Y. enterocolitica were sequenced, and single (ProP− ProU+ and ProP+ ProU−) and double (ProP− ProU−) mutants were generated. Upon exposure to osmotic or chill stress, the single and double mutants demonstrated a reduction in betaine uptake compared to that in the wild type, suggesting that proP and proU play a role in betaine uptake during osmotic and chill stress responses of Y. enterocolitica.

scholarly journals An ABA-GA bistable switch can account for natural variation in the variability of Arabidopsis seed germination time

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Katie Abley ◽  
Pau Formosa-Jordan ◽  
Hugo Tavares ◽  
Emily YT Chan ◽  
Mana Afsharinafar ◽  
...  
Keyword(s):  
Seed Germination ◽  
Phenotypic Variability ◽  
Time Variability ◽  
Bistable Switch ◽  
Germination Time ◽  
Arabidopsis Seed ◽  
Aba Sensitivity ◽  
Recombinant Inbred Population ◽  
Underlying Mechanisms

Genetically identical plants growing in the same conditions can display heterogeneous phenotypes. Here we use Arabidopsis seed germination time as a model system to examine phenotypic variability and its underlying mechanisms. We show extensive variation in seed germination time variability between Arabidopsis accessions and use a multiparent recombinant inbred population to identify two genetic loci involved in this trait. Both loci include genes implicated in modulating abscisic acid (ABA) sensitivity. Mutually antagonistic regulation between ABA, which represses germination, and gibberellic acid (GA), which promotes germination, underlies the decision to germinate and can act as a bistable switch. A simple stochastic model of the ABA-GA network shows that modulating ABA sensitivity can generate the range of germination time distributions we observe experimentally. We validate the model by testing its predictions on the effects of exogenous hormone addition. Our work provides a foundation for understanding the mechanism and functional role of phenotypic variability in germination time.

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